Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10020 and DZA65_RS10025 are separated by 280 nucleotides DZA65_RS10025 and DZA65_RS10030 overlap by 7 nucleotides DZA65_RS10030 and DZA65_RS10035 overlap by 19 nucleotides DZA65_RS10035 and DZA65_RS10040 are separated by 6 nucleotides
DZA65_RS10020: DZA65_RS10020 - hypothetical protein, at 2,122,354 to 2,122,956
_RS10020
DZA65_RS10025: DZA65_RS10025 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 2,123,237 to 2,123,851
_RS10025
DZA65_RS10030: DZA65_RS10030 - imidazole glycerol phosphate synthase subunit HisF, at 2,123,845 to 2,124,621
_RS10030
DZA65_RS10035: DZA65_RS10035 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,124,603 to 2,125,340
_RS10035
DZA65_RS10040: DZA65_RS10040 - imidazole glycerol phosphate synthase subunit HisH, at 2,125,347 to 2,125,937
_RS10040
Position (kb)
2123
2124
2125 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2122.845 kb on + strand, within DZA65_RS10020 at 2122.845 kb on + strand, within DZA65_RS10020 at 2122.845 kb on + strand, within DZA65_RS10020 at 2122.845 kb on + strand, within DZA65_RS10020 at 2122.845 kb on + strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.860 kb on + strand, within DZA65_RS10020 at 2122.951 kb on + strand at 2122.951 kb on + strand at 2122.951 kb on + strand at 2122.952 kb on - strand at 2122.952 kb on - strand at 2122.952 kb on - strand at 2122.967 kb on + strand at 2122.989 kb on + strand at 2123.019 kb on + strand at 2123.101 kb on - strand at 2123.101 kb on - strand at 2123.156 kb on + strand at 2123.157 kb on - strand at 2123.275 kb on + strand at 2123.319 kb on + strand, within DZA65_RS10025 at 2123.319 kb on + strand, within DZA65_RS10025 at 2123.320 kb on - strand, within DZA65_RS10025 at 2123.436 kb on + strand, within DZA65_RS10025 at 2123.437 kb on - strand, within DZA65_RS10025 at 2123.451 kb on + strand, within DZA65_RS10025 at 2123.451 kb on + strand at 2123.477 kb on - strand, within DZA65_RS10025 at 2123.620 kb on + strand at 2123.691 kb on + strand, within DZA65_RS10025 at 2123.727 kb on - strand, within DZA65_RS10025 at 2123.745 kb on + strand, within DZA65_RS10025 at 2123.745 kb on + strand, within DZA65_RS10025 at 2123.783 kb on + strand, within DZA65_RS10025 at 2123.783 kb on + strand, within DZA65_RS10025 at 2123.784 kb on - strand, within DZA65_RS10025 at 2123.784 kb on - strand at 2123.784 kb on - strand, within DZA65_RS10025 at 2123.878 kb on - strand at 2124.159 kb on + strand, within DZA65_RS10030 at 2124.188 kb on + strand, within DZA65_RS10030 at 2124.188 kb on + strand, within DZA65_RS10030 at 2124.256 kb on + strand, within DZA65_RS10030 at 2124.468 kb on + strand, within DZA65_RS10030 at 2124.473 kb on + strand, within DZA65_RS10030 at 2124.571 kb on + strand at 2124.571 kb on + strand at 2124.699 kb on + strand, within DZA65_RS10035 at 2124.794 kb on + strand, within DZA65_RS10035 at 2124.794 kb on + strand, within DZA65_RS10035 at 2124.870 kb on + strand, within DZA65_RS10035 at 2125.293 kb on + strand at 2125.348 kb on + strand at 2125.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 2,122,845 + DZA65_RS10020 0.81 +0.2 2,122,845 + DZA65_RS10020 0.81 +1.9 2,122,845 + DZA65_RS10020 0.81 +0.9 2,122,845 + DZA65_RS10020 0.81 +0.1 2,122,845 + DZA65_RS10020 0.81 +0.3 2,122,846 - DZA65_RS10020 0.82 -0.1 2,122,846 - DZA65_RS10020 0.82 -0.3 2,122,846 - DZA65_RS10020 0.82 -0.9 2,122,846 - DZA65_RS10020 0.82 +1.0 2,122,846 - DZA65_RS10020 0.82 -1.2 2,122,846 - DZA65_RS10020 0.82 +0.3 2,122,846 - DZA65_RS10020 0.82 -2.5 2,122,846 - DZA65_RS10020 0.82 +0.6 2,122,846 - DZA65_RS10020 0.82 +1.6 2,122,846 - DZA65_RS10020 0.82 +0.1 2,122,860 + DZA65_RS10020 0.84 -0.1 2,122,951 + +1.9 2,122,951 + +1.7 2,122,951 + +0.9 2,122,952 - +1.0 2,122,952 - +0.3 2,122,952 - +1.3 2,122,967 + +0.5 2,122,989 + -0.6 2,123,019 + +0.7 2,123,101 - -0.1 2,123,101 - +0.9 2,123,156 + -0.0 2,123,157 - +1.1 2,123,275 + -0.4 2,123,319 + DZA65_RS10025 0.13 +0.1 2,123,319 + DZA65_RS10025 0.13 -1.7 2,123,320 - DZA65_RS10025 0.13 +1.0 2,123,436 + DZA65_RS10025 0.32 -2.2 2,123,437 - DZA65_RS10025 0.33 -1.0 2,123,451 + DZA65_RS10025 0.35 -0.3 2,123,451 + -2.2 2,123,477 - DZA65_RS10025 0.39 -0.4 2,123,620 + -1.9 2,123,691 + DZA65_RS10025 0.74 +0.1 2,123,727 - DZA65_RS10025 0.80 -0.6 2,123,745 + DZA65_RS10025 0.83 -1.1 2,123,745 + DZA65_RS10025 0.83 -0.5 2,123,783 + DZA65_RS10025 0.89 -0.6 2,123,783 + DZA65_RS10025 0.89 +0.6 2,123,784 - DZA65_RS10025 0.89 -0.1 2,123,784 - +1.1 2,123,784 - DZA65_RS10025 0.89 -0.1 2,123,878 - -1.3 2,124,159 + DZA65_RS10030 0.40 -0.0 2,124,188 + DZA65_RS10030 0.44 -1.3 2,124,188 + DZA65_RS10030 0.44 -0.3 2,124,256 + DZA65_RS10030 0.53 -1.9 2,124,468 + DZA65_RS10030 0.80 -1.3 2,124,473 + DZA65_RS10030 0.81 -1.6 2,124,571 + -0.6 2,124,571 + -0.3 2,124,699 + DZA65_RS10035 0.13 -0.9 2,124,794 + DZA65_RS10035 0.26 -0.9 2,124,794 + DZA65_RS10035 0.26 +0.1 2,124,870 + DZA65_RS10035 0.36 -1.2 2,125,293 + -0.3 2,125,348 + -1.5 2,125,371 + -0.1
Or see this region's nucleotide sequence