Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS05895 and DZA65_RS05900 are separated by 22 nucleotides DZA65_RS05900 and DZA65_RS05905 are separated by 60 nucleotides DZA65_RS05905 and DZA65_RS05910 overlap by 20 nucleotides DZA65_RS05910 and DZA65_RS05915 are separated by 86 nucleotides
DZA65_RS05895: DZA65_RS05895 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,216,909 to 1,217,382
_RS05895
DZA65_RS05900: DZA65_RS05900 - transcription antitermination factor NusB, at 1,217,405 to 1,217,824
_RS05900
DZA65_RS05905: DZA65_RS05905 - thiamine-phosphate kinase, at 1,217,885 to 1,218,859
_RS05905
DZA65_RS05910: DZA65_RS05910 - phosphatidylglycerophosphatase A, at 1,218,840 to 1,219,352
_RS05910
DZA65_RS05915: DZA65_RS05915 - 1-deoxy-D-xylulose-5-phosphate synthase, at 1,219,439 to 1,221,301
_RS05915
Position (kb)
1217
1218
1219 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1216.888 kb on + strand at 1216.888 kb on + strand at 1217.543 kb on + strand, within DZA65_RS05900 at 1217.801 kb on + strand at 1217.822 kb on + strand at 1217.865 kb on + strand at 1219.341 kb on + strand at 1219.341 kb on + strand at 1219.341 kb on + strand at 1219.341 kb on + strand at 1219.341 kb on + strand at 1219.368 kb on - strand at 1219.368 kb on - strand at 1219.368 kb on - strand at 1219.369 kb on + strand at 1219.370 kb on - strand at 1219.370 kb on - strand at 1219.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 1,216,888 + +0.0 1,216,888 + +0.9 1,217,543 + DZA65_RS05900 0.33 +1.0 1,217,801 + -0.7 1,217,822 + -0.8 1,217,865 + +0.0 1,219,341 + +0.8 1,219,341 + -1.1 1,219,341 + +0.9 1,219,341 + -2.6 1,219,341 + -0.6 1,219,368 - +0.7 1,219,368 - -1.6 1,219,368 - -0.6 1,219,369 + +1.3 1,219,370 - +2.8 1,219,370 - -1.1 1,219,403 - -1.1
Or see this region's nucleotide sequence