Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS00835 and DZA65_RS00840 are separated by 226 nucleotides DZA65_RS00840 and DZA65_RS00845 are separated by 99 nucleotides DZA65_RS00845 and DZA65_RS00850 overlap by 1 nucleotides
DZA65_RS00835: DZA65_RS00835 - murein hydrolase activator EnvC, at 187,300 to 188,574
_RS00835
DZA65_RS00840: DZA65_RS00840 - rhodanese-like domain-containing protein, at 188,801 to 189,232
_RS00840
DZA65_RS00845: DZA65_RS00845 - protein-export chaperone SecB, at 189,332 to 189,802
_RS00845
DZA65_RS00850: DZA65_RS00850 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 189,802 to 190,821
_RS00850
Position (kb) 189
190 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 188.488 kb on + strand at 188.521 kb on + strand at 188.522 kb on - strand at 188.522 kb on - strand at 188.585 kb on + strand at 188.585 kb on + strand at 188.585 kb on + strand at 188.586 kb on - strand at 188.586 kb on - strand at 188.586 kb on - strand at 188.632 kb on + strand at 188.632 kb on + strand at 188.633 kb on - strand at 188.673 kb on + strand at 188.693 kb on + strand at 188.693 kb on + strand at 188.716 kb on - strand at 188.724 kb on + strand at 188.724 kb on + strand at 188.724 kb on + strand at 188.725 kb on - strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.741 kb on - strand at 188.741 kb on - strand at 188.741 kb on - strand at 188.741 kb on - strand at 188.741 kb on - strand at 188.741 kb on - strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.745 kb on - strand at 188.746 kb on + strand at 188.746 kb on + strand at 188.746 kb on + strand at 188.761 kb on + strand at 188.761 kb on + strand at 188.761 kb on + strand at 188.761 kb on + strand at 188.774 kb on + strand at 188.774 kb on + strand at 188.774 kb on + strand at 188.774 kb on + strand at 188.774 kb on + strand at 188.793 kb on + strand at 188.812 kb on + strand at 188.812 kb on + strand at 188.812 kb on + strand at 188.812 kb on + strand at 188.812 kb on + strand at 188.813 kb on - strand at 188.884 kb on + strand, within DZA65_RS00840 at 188.896 kb on + strand, within DZA65_RS00840 at 188.896 kb on + strand, within DZA65_RS00840 at 188.897 kb on - strand, within DZA65_RS00840 at 188.947 kb on + strand, within DZA65_RS00840 at 188.948 kb on - strand, within DZA65_RS00840 at 188.950 kb on + strand, within DZA65_RS00840 at 188.970 kb on + strand, within DZA65_RS00840 at 189.007 kb on + strand, within DZA65_RS00840 at 189.007 kb on + strand, within DZA65_RS00840 at 189.007 kb on + strand, within DZA65_RS00840 at 189.008 kb on - strand, within DZA65_RS00840 at 189.267 kb on + strand at 189.267 kb on + strand at 189.376 kb on + strand at 189.377 kb on - strand at 189.380 kb on + strand, within DZA65_RS00845 at 189.749 kb on + strand, within DZA65_RS00845 at 189.749 kb on + strand, within DZA65_RS00845 at 189.749 kb on + strand, within DZA65_RS00845 at 189.749 kb on + strand, within DZA65_RS00845 at 189.822 kb on + strand at 189.822 kb on + strand at 189.823 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 188,488 + -0.9 188,521 + +0.6 188,522 - +1.1 188,522 - -0.9 188,585 + -0.3 188,585 + +0.5 188,585 + +0.2 188,586 - +1.0 188,586 - -2.6 188,586 - +0.3 188,632 + -0.5 188,632 + -0.2 188,633 - +0.8 188,673 + +0.2 188,693 + -0.1 188,693 + -0.2 188,716 - -0.5 188,724 + -1.0 188,724 + -0.6 188,724 + +1.1 188,725 - +0.6 188,740 + -0.5 188,740 + -1.2 188,740 + -0.4 188,740 + -1.8 188,740 + -0.2 188,740 + +1.0 188,740 + +0.1 188,741 - +0.2 188,741 - -0.4 188,741 - +1.0 188,741 - +0.6 188,741 - -0.6 188,741 - -0.5 188,744 + +1.7 188,744 + -0.2 188,744 + -2.6 188,744 + +0.1 188,744 + +0.1 188,744 + -2.6 188,744 + +1.4 188,744 + +1.0 188,745 - -0.8 188,746 + -1.2 188,746 + +0.8 188,746 + -1.8 188,761 + +0.5 188,761 + -3.3 188,761 + -1.2 188,761 + +0.2 188,774 + +0.8 188,774 + -0.6 188,774 + +1.0 188,774 + -0.2 188,774 + -1.0 188,793 + -1.8 188,812 + -0.8 188,812 + -0.1 188,812 + -0.1 188,812 + -0.1 188,812 + -0.2 188,813 - -0.3 188,884 + DZA65_RS00840 0.19 -1.2 188,896 + DZA65_RS00840 0.22 +1.7 188,896 + DZA65_RS00840 0.22 -0.5 188,897 - DZA65_RS00840 0.22 +0.1 188,947 + DZA65_RS00840 0.34 +0.4 188,948 - DZA65_RS00840 0.34 -0.2 188,950 + DZA65_RS00840 0.34 +0.5 188,970 + DZA65_RS00840 0.39 +0.8 189,007 + DZA65_RS00840 0.48 -1.6 189,007 + DZA65_RS00840 0.48 +0.6 189,007 + DZA65_RS00840 0.48 -2.6 189,008 - DZA65_RS00840 0.48 -0.5 189,267 + +0.8 189,267 + -0.8 189,376 + -0.2 189,377 - +0.5 189,380 + DZA65_RS00845 0.10 -1.2 189,749 + DZA65_RS00845 0.89 -0.4 189,749 + DZA65_RS00845 0.89 -1.2 189,749 + DZA65_RS00845 0.89 -1.1 189,749 + DZA65_RS00845 0.89 +0.1 189,822 + +0.8 189,822 + -1.6 189,823 - +0.1
Or see this region's nucleotide sequence