Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS05890 and DZA65_RS05895 are separated by 120 nucleotides DZA65_RS05895 and DZA65_RS05900 are separated by 22 nucleotides DZA65_RS05900 and DZA65_RS05905 are separated by 60 nucleotides
DZA65_RS05890: DZA65_RS05890 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 1,215,676 to 1,216,788
_RS05890
DZA65_RS05895: DZA65_RS05895 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,216,909 to 1,217,382
_RS05895
DZA65_RS05900: DZA65_RS05900 - transcription antitermination factor NusB, at 1,217,405 to 1,217,824
_RS05900
DZA65_RS05905: DZA65_RS05905 - thiamine-phosphate kinase, at 1,217,885 to 1,218,859
_RS05905
Position (kb)
1216
1217
1218 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1216.776 kb on - strand at 1216.786 kb on + strand at 1216.787 kb on - strand at 1216.787 kb on - strand at 1216.858 kb on + strand at 1216.858 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.863 kb on + strand at 1216.888 kb on + strand at 1216.888 kb on + strand at 1217.543 kb on + strand, within DZA65_RS05900 at 1217.801 kb on + strand at 1217.822 kb on + strand at 1217.865 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 1,216,776 - -1.3 1,216,786 + -1.9 1,216,787 - -0.9 1,216,787 - -2.8 1,216,858 + -1.5 1,216,858 + -0.3 1,216,863 + +0.9 1,216,863 + -0.3 1,216,863 + -2.3 1,216,863 + -1.0 1,216,863 + +0.2 1,216,863 + -0.1 1,216,863 + +1.1 1,216,863 + -0.1 1,216,863 + -0.1 1,216,863 + +0.5 1,216,863 + +0.2 1,216,863 + +0.2 1,216,888 + +0.7 1,216,888 + +0.7 1,217,543 + DZA65_RS05900 0.33 -0.6 1,217,801 + -0.1 1,217,822 + +0.2 1,217,865 + -1.3
Or see this region's nucleotide sequence