Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS20830 and DZA65_RS20835 are separated by 97 nucleotides DZA65_RS20835 and DZA65_RS20840 overlap by 4 nucleotides DZA65_RS20840 and DZA65_RS20845 are separated by 269 nucleotides
DZA65_RS20830: DZA65_RS20830 - phosphoenolpyruvate carboxykinase (ATP), at 4,493,751 to 4,495,370
_RS20830
DZA65_RS20835: DZA65_RS20835 - two-component system sensor histidine kinase EnvZ, at 4,495,468 to 4,496,880
_RS20835
DZA65_RS20840: DZA65_RS20840 - two-component system response regulator OmpR, at 4,496,877 to 4,497,596
_RS20840
DZA65_RS20845: DZA65_RS20845 - transcription elongation factor GreB, at 4,497,866 to 4,498,384
_RS20845
Position (kb)
4495
4496
4497 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4494.603 kb on + strand, within DZA65_RS20830 at 4494.603 kb on + strand, within DZA65_RS20830 at 4494.603 kb on + strand, within DZA65_RS20830 at 4494.644 kb on + strand, within DZA65_RS20830 at 4494.644 kb on + strand, within DZA65_RS20830 at 4494.645 kb on - strand, within DZA65_RS20830 at 4494.648 kb on + strand, within DZA65_RS20830 at 4494.648 kb on + strand at 4494.649 kb on - strand, within DZA65_RS20830 at 4494.649 kb on - strand at 4494.649 kb on - strand, within DZA65_RS20830 at 4494.754 kb on - strand, within DZA65_RS20830 at 4494.762 kb on + strand, within DZA65_RS20830 at 4494.762 kb on + strand, within DZA65_RS20830 at 4494.762 kb on + strand, within DZA65_RS20830 at 4494.762 kb on + strand, within DZA65_RS20830 at 4494.762 kb on + strand, within DZA65_RS20830 at 4494.762 kb on + strand, within DZA65_RS20830 at 4494.762 kb on + strand, within DZA65_RS20830 at 4494.762 kb on + strand, within DZA65_RS20830 at 4494.762 kb on + strand, within DZA65_RS20830 at 4494.763 kb on - strand, within DZA65_RS20830 at 4494.763 kb on - strand, within DZA65_RS20830 at 4494.763 kb on - strand, within DZA65_RS20830 at 4494.763 kb on - strand, within DZA65_RS20830 at 4494.763 kb on - strand, within DZA65_RS20830 at 4494.763 kb on - strand, within DZA65_RS20830 at 4494.763 kb on - strand, within DZA65_RS20830 at 4494.763 kb on - strand, within DZA65_RS20830 at 4494.763 kb on - strand, within DZA65_RS20830 at 4494.763 kb on - strand, within DZA65_RS20830 at 4494.889 kb on - strand, within DZA65_RS20830 at 4495.056 kb on + strand, within DZA65_RS20830 at 4495.056 kb on + strand, within DZA65_RS20830 at 4495.057 kb on - strand, within DZA65_RS20830 at 4495.057 kb on - strand, within DZA65_RS20830 at 4495.057 kb on - strand, within DZA65_RS20830 at 4495.175 kb on + strand, within DZA65_RS20830 at 4495.175 kb on + strand, within DZA65_RS20830 at 4495.175 kb on + strand, within DZA65_RS20830 at 4495.175 kb on + strand, within DZA65_RS20830 at 4495.176 kb on - strand, within DZA65_RS20830 at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.245 kb on + strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.246 kb on - strand at 4495.248 kb on - strand at 4495.298 kb on + strand at 4495.298 kb on + strand at 4495.299 kb on - strand at 4495.317 kb on + strand at 4495.317 kb on + strand at 4495.317 kb on + strand at 4495.317 kb on + strand at 4495.318 kb on - strand at 4495.318 kb on - strand at 4495.318 kb on - strand at 4495.318 kb on - strand at 4495.318 kb on - strand at 4495.318 kb on - strand at 4495.318 kb on - strand at 4495.318 kb on - strand at 4495.318 kb on - strand at 4495.318 kb on - strand at 4495.318 kb on - strand at 4495.368 kb on + strand at 4495.378 kb on - strand at 4495.381 kb on + strand at 4495.420 kb on + strand at 4495.461 kb on + strand at 4495.469 kb on + strand at 4495.573 kb on + strand at 4495.573 kb on + strand at 4495.574 kb on - strand at 4495.574 kb on - strand at 4495.574 kb on - strand at 4495.577 kb on + strand at 4495.578 kb on - strand at 4495.578 kb on - strand at 4495.578 kb on - strand at 4495.645 kb on - strand, within DZA65_RS20835 at 4495.645 kb on - strand, within DZA65_RS20835 at 4495.645 kb on - strand at 4495.648 kb on - strand, within DZA65_RS20835 at 4495.895 kb on - strand at 4495.938 kb on - strand, within DZA65_RS20835 at 4496.021 kb on + strand, within DZA65_RS20835 at 4496.022 kb on - strand, within DZA65_RS20835 at 4496.049 kb on + strand, within DZA65_RS20835 at 4496.050 kb on - strand, within DZA65_RS20835 at 4496.068 kb on - strand, within DZA65_RS20835 at 4496.088 kb on - strand, within DZA65_RS20835 at 4496.178 kb on + strand, within DZA65_RS20835 at 4496.258 kb on + strand, within DZA65_RS20835 at 4496.258 kb on + strand, within DZA65_RS20835 at 4496.281 kb on - strand, within DZA65_RS20835 at 4496.281 kb on - strand, within DZA65_RS20835 at 4496.283 kb on + strand, within DZA65_RS20835 at 4496.283 kb on + strand, within DZA65_RS20835 at 4496.284 kb on - strand, within DZA65_RS20835 at 4496.284 kb on - strand at 4496.302 kb on - strand, within DZA65_RS20835 at 4496.374 kb on + strand, within DZA65_RS20835 at 4496.374 kb on + strand, within DZA65_RS20835 at 4496.375 kb on - strand, within DZA65_RS20835 at 4496.375 kb on - strand, within DZA65_RS20835 at 4496.379 kb on + strand, within DZA65_RS20835 at 4496.380 kb on - strand, within DZA65_RS20835 at 4496.391 kb on + strand, within DZA65_RS20835 at 4496.391 kb on + strand, within DZA65_RS20835 at 4496.391 kb on + strand, within DZA65_RS20835 at 4496.392 kb on - strand, within DZA65_RS20835 at 4496.392 kb on - strand, within DZA65_RS20835 at 4496.393 kb on + strand, within DZA65_RS20835 at 4496.567 kb on + strand, within DZA65_RS20835 at 4496.567 kb on + strand, within DZA65_RS20835 at 4496.567 kb on + strand, within DZA65_RS20835 at 4496.568 kb on - strand, within DZA65_RS20835 at 4496.568 kb on - strand, within DZA65_RS20835 at 4496.568 kb on - strand, within DZA65_RS20835 at 4496.568 kb on - strand, within DZA65_RS20835 at 4496.615 kb on + strand, within DZA65_RS20835 at 4496.616 kb on - strand, within DZA65_RS20835 at 4496.639 kb on + strand, within DZA65_RS20835 at 4496.726 kb on + strand, within DZA65_RS20835 at 4496.727 kb on - strand, within DZA65_RS20835 at 4496.775 kb on + strand at 4496.776 kb on - strand at 4496.776 kb on - strand at 4496.776 kb on - strand at 4496.789 kb on + strand at 4496.789 kb on + strand at 4496.789 kb on + strand at 4496.790 kb on - strand at 4496.820 kb on + strand at 4496.838 kb on + strand at 4496.838 kb on + strand at 4496.886 kb on - strand at 4496.934 kb on - strand at 4496.934 kb on - strand at 4496.934 kb on - strand at 4496.935 kb on + strand at 4496.961 kb on + strand, within DZA65_RS20840 at 4496.961 kb on + strand at 4496.961 kb on + strand, within DZA65_RS20840 at 4497.015 kb on - strand, within DZA65_RS20840 at 4497.015 kb on - strand, within DZA65_RS20840 at 4497.016 kb on + strand, within DZA65_RS20840 at 4497.016 kb on + strand at 4497.017 kb on - strand, within DZA65_RS20840 at 4497.261 kb on + strand, within DZA65_RS20840 at 4497.262 kb on - strand, within DZA65_RS20840 at 4497.292 kb on + strand, within DZA65_RS20840 at 4497.292 kb on + strand, within DZA65_RS20840 at 4497.293 kb on - strand, within DZA65_RS20840 at 4497.293 kb on - strand, within DZA65_RS20840 at 4497.293 kb on - strand, within DZA65_RS20840 at 4497.356 kb on + strand, within DZA65_RS20840 at 4497.356 kb on + strand, within DZA65_RS20840 at 4497.558 kb on - strand at 4497.618 kb on + strand at 4497.618 kb on + strand at 4497.618 kb on + strand at 4497.618 kb on + strand at 4497.618 kb on + strand at 4497.618 kb on + strand at 4497.618 kb on + strand at 4497.619 kb on - strand at 4497.619 kb on - strand at 4497.619 kb on - strand at 4497.619 kb on - strand at 4497.619 kb on - strand at 4497.619 kb on - strand at 4497.638 kb on + strand at 4497.639 kb on - strand at 4497.658 kb on + strand at 4497.659 kb on - strand at 4497.659 kb on - strand at 4497.659 kb on - strand at 4497.699 kb on + strand at 4497.700 kb on - strand at 4497.700 kb on - strand at 4497.701 kb on + strand at 4497.701 kb on + strand at 4497.701 kb on + strand at 4497.701 kb on + strand at 4497.701 kb on + strand at 4497.701 kb on + strand at 4497.701 kb on + strand at 4497.702 kb on - strand at 4497.702 kb on - strand at 4497.702 kb on - strand at 4497.702 kb on - strand at 4497.702 kb on - strand at 4497.702 kb on - strand at 4497.703 kb on + strand at 4497.703 kb on + strand at 4497.703 kb on + strand at 4497.703 kb on + strand at 4497.703 kb on + strand at 4497.703 kb on + strand at 4497.703 kb on + strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.704 kb on - strand at 4497.708 kb on + strand at 4497.739 kb on - strand at 4497.739 kb on - strand at 4497.739 kb on - strand at 4497.745 kb on + strand at 4497.745 kb on + strand at 4497.745 kb on + strand at 4497.745 kb on + strand at 4497.745 kb on + strand at 4497.745 kb on + strand at 4497.745 kb on + strand at 4497.746 kb on - strand at 4497.746 kb on - strand at 4497.746 kb on - strand at 4497.746 kb on - strand at 4497.746 kb on - strand at 4497.746 kb on - strand at 4497.746 kb on - strand at 4497.746 kb on - strand at 4497.805 kb on + strand at 4497.805 kb on + strand at 4497.806 kb on - strand at 4497.810 kb on + strand at 4497.810 kb on + strand at 4497.810 kb on + strand at 4497.810 kb on + strand at 4497.811 kb on - strand at 4497.811 kb on - strand at 4497.828 kb on + strand at 4497.828 kb on + strand at 4497.828 kb on + strand at 4497.828 kb on + strand at 4497.829 kb on - strand at 4497.829 kb on - strand at 4497.831 kb on - strand at 4497.840 kb on - strand at 4497.848 kb on + strand at 4497.848 kb on + strand at 4497.849 kb on - strand at 4497.849 kb on - strand at 4497.849 kb on - strand at 4497.866 kb on - strand at 4497.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 4,494,603 + DZA65_RS20830 0.53 +0.4 4,494,603 + DZA65_RS20830 0.53 +0.2 4,494,603 + DZA65_RS20830 0.53 +2.0 4,494,644 + DZA65_RS20830 0.55 +1.0 4,494,644 + DZA65_RS20830 0.55 +0.2 4,494,645 - DZA65_RS20830 0.55 -2.5 4,494,648 + DZA65_RS20830 0.55 -0.7 4,494,648 + -1.0 4,494,649 - DZA65_RS20830 0.55 -1.1 4,494,649 - +0.0 4,494,649 - DZA65_RS20830 0.55 +0.8 4,494,754 - DZA65_RS20830 0.62 +0.2 4,494,762 + DZA65_RS20830 0.62 -0.8 4,494,762 + DZA65_RS20830 0.62 -0.5 4,494,762 + DZA65_RS20830 0.62 -1.1 4,494,762 + DZA65_RS20830 0.62 -3.0 4,494,762 + DZA65_RS20830 0.62 -0.0 4,494,762 + DZA65_RS20830 0.62 +0.8 4,494,762 + DZA65_RS20830 0.62 -1.3 4,494,762 + DZA65_RS20830 0.62 -1.0 4,494,762 + DZA65_RS20830 0.62 -1.1 4,494,763 - DZA65_RS20830 0.62 -0.1 4,494,763 - DZA65_RS20830 0.62 -1.4 4,494,763 - DZA65_RS20830 0.62 -1.2 4,494,763 - DZA65_RS20830 0.62 -0.5 4,494,763 - DZA65_RS20830 0.62 +0.6 4,494,763 - DZA65_RS20830 0.62 +0.0 4,494,763 - DZA65_RS20830 0.62 -0.4 4,494,763 - DZA65_RS20830 0.62 -1.5 4,494,763 - DZA65_RS20830 0.62 -0.5 4,494,763 - DZA65_RS20830 0.62 +0.4 4,494,889 - DZA65_RS20830 0.70 +0.6 4,495,056 + DZA65_RS20830 0.81 +0.7 4,495,056 + DZA65_RS20830 0.81 -0.0 4,495,057 - DZA65_RS20830 0.81 +0.3 4,495,057 - DZA65_RS20830 0.81 +0.1 4,495,057 - DZA65_RS20830 0.81 +1.3 4,495,175 + DZA65_RS20830 0.88 -0.8 4,495,175 + DZA65_RS20830 0.88 +0.3 4,495,175 + DZA65_RS20830 0.88 -0.8 4,495,175 + DZA65_RS20830 0.88 +0.7 4,495,176 - DZA65_RS20830 0.88 -0.5 4,495,245 + +0.6 4,495,245 + +0.6 4,495,245 + +2.6 4,495,245 + +1.4 4,495,245 + +0.0 4,495,245 + +0.0 4,495,245 + +0.8 4,495,245 + -0.1 4,495,245 + -0.5 4,495,245 + -1.5 4,495,245 + +0.7 4,495,245 + -2.1 4,495,245 + +1.0 4,495,246 - +0.5 4,495,246 - +0.3 4,495,246 - +0.7 4,495,246 - +0.2 4,495,246 - +0.7 4,495,246 - +2.4 4,495,246 - -1.1 4,495,246 - -1.5 4,495,246 - -0.7 4,495,246 - -0.3 4,495,246 - -0.1 4,495,246 - +0.0 4,495,246 - -0.2 4,495,246 - -0.1 4,495,248 - -0.5 4,495,298 + +2.8 4,495,298 + +0.0 4,495,299 - +0.1 4,495,317 + -0.6 4,495,317 + +1.5 4,495,317 + +1.0 4,495,317 + -1.0 4,495,318 - -0.3 4,495,318 - -2.0 4,495,318 - +1.0 4,495,318 - -1.8 4,495,318 - -0.3 4,495,318 - -1.0 4,495,318 - -0.8 4,495,318 - -1.3 4,495,318 - -0.3 4,495,318 - +1.8 4,495,318 - -0.5 4,495,368 + +1.0 4,495,378 - +0.5 4,495,381 + -1.7 4,495,420 + -0.5 4,495,461 + -0.3 4,495,469 + +3.4 4,495,573 + +2.2 4,495,573 + +1.0 4,495,574 - +1.5 4,495,574 - -1.0 4,495,574 - -1.7 4,495,577 + +0.6 4,495,578 - +0.6 4,495,578 - +0.1 4,495,578 - +1.0 4,495,645 - DZA65_RS20835 0.13 -0.5 4,495,645 - DZA65_RS20835 0.13 -1.0 4,495,645 - -0.3 4,495,648 - DZA65_RS20835 0.13 +0.0 4,495,895 - +1.0 4,495,938 - DZA65_RS20835 0.33 +0.8 4,496,021 + DZA65_RS20835 0.39 -2.3 4,496,022 - DZA65_RS20835 0.39 -0.6 4,496,049 + DZA65_RS20835 0.41 -0.5 4,496,050 - DZA65_RS20835 0.41 -0.2 4,496,068 - DZA65_RS20835 0.42 -1.3 4,496,088 - DZA65_RS20835 0.44 -0.7 4,496,178 + DZA65_RS20835 0.50 -1.0 4,496,258 + DZA65_RS20835 0.56 -0.3 4,496,258 + DZA65_RS20835 0.56 +0.5 4,496,281 - DZA65_RS20835 0.58 -0.1 4,496,281 - DZA65_RS20835 0.58 -2.3 4,496,283 + DZA65_RS20835 0.58 -1.0 4,496,283 + DZA65_RS20835 0.58 +0.2 4,496,284 - DZA65_RS20835 0.58 -0.8 4,496,284 - -0.5 4,496,302 - DZA65_RS20835 0.59 -1.5 4,496,374 + DZA65_RS20835 0.64 -1.0 4,496,374 + DZA65_RS20835 0.64 -2.4 4,496,375 - DZA65_RS20835 0.64 -0.3 4,496,375 - DZA65_RS20835 0.64 -1.9 4,496,379 + DZA65_RS20835 0.64 +1.0 4,496,380 - DZA65_RS20835 0.65 +0.5 4,496,391 + DZA65_RS20835 0.65 -1.9 4,496,391 + DZA65_RS20835 0.65 +1.0 4,496,391 + DZA65_RS20835 0.65 +0.6 4,496,392 - DZA65_RS20835 0.65 +0.8 4,496,392 - DZA65_RS20835 0.65 -0.5 4,496,393 + DZA65_RS20835 0.65 -0.5 4,496,567 + DZA65_RS20835 0.78 +0.0 4,496,567 + DZA65_RS20835 0.78 -2.3 4,496,567 + DZA65_RS20835 0.78 -1.3 4,496,568 - DZA65_RS20835 0.78 -1.2 4,496,568 - DZA65_RS20835 0.78 -0.5 4,496,568 - DZA65_RS20835 0.78 +0.6 4,496,568 - DZA65_RS20835 0.78 +0.7 4,496,615 + DZA65_RS20835 0.81 -1.8 4,496,616 - DZA65_RS20835 0.81 -1.3 4,496,639 + DZA65_RS20835 0.83 +1.0 4,496,726 + DZA65_RS20835 0.89 +0.0 4,496,727 - DZA65_RS20835 0.89 -0.2 4,496,775 + -1.0 4,496,776 - -1.8 4,496,776 - +0.6 4,496,776 - -0.8 4,496,789 + +1.7 4,496,789 + -2.0 4,496,789 + +1.0 4,496,790 - -0.1 4,496,820 + -0.2 4,496,838 + +0.7 4,496,838 + -1.3 4,496,886 - +0.0 4,496,934 - +1.6 4,496,934 - +0.5 4,496,934 - -0.5 4,496,935 + +1.0 4,496,961 + DZA65_RS20840 0.12 +0.7 4,496,961 + +0.5 4,496,961 + DZA65_RS20840 0.12 -1.7 4,497,015 - DZA65_RS20840 0.19 -2.1 4,497,015 - DZA65_RS20840 0.19 -1.8 4,497,016 + DZA65_RS20840 0.19 -0.1 4,497,016 + -1.0 4,497,017 - DZA65_RS20840 0.19 -0.5 4,497,261 + DZA65_RS20840 0.53 -0.5 4,497,262 - DZA65_RS20840 0.53 -0.3 4,497,292 + DZA65_RS20840 0.58 +0.2 4,497,292 + DZA65_RS20840 0.58 -1.7 4,497,293 - DZA65_RS20840 0.58 -1.0 4,497,293 - DZA65_RS20840 0.58 +1.3 4,497,293 - DZA65_RS20840 0.58 +0.3 4,497,356 + DZA65_RS20840 0.67 -1.5 4,497,356 + DZA65_RS20840 0.67 -1.0 4,497,558 - +3.2 4,497,618 + -0.2 4,497,618 + -0.1 4,497,618 + -0.4 4,497,618 + +0.5 4,497,618 + -1.5 4,497,618 + -1.3 4,497,618 + +1.2 4,497,619 - +0.5 4,497,619 - -1.2 4,497,619 - -1.3 4,497,619 - +0.2 4,497,619 - -1.3 4,497,619 - +0.0 4,497,638 + -1.0 4,497,639 - +1.8 4,497,658 + +1.1 4,497,659 - -0.2 4,497,659 - +0.6 4,497,659 - +0.1 4,497,699 + -0.7 4,497,700 - +0.5 4,497,700 - -1.3 4,497,701 + -1.4 4,497,701 + +3.4 4,497,701 + +0.5 4,497,701 + -1.8 4,497,701 + +0.5 4,497,701 + -0.8 4,497,701 + -1.0 4,497,702 - -1.4 4,497,702 - +1.0 4,497,702 - -1.1 4,497,702 - -0.7 4,497,702 - -0.5 4,497,702 - +0.3 4,497,703 + -0.5 4,497,703 + -0.3 4,497,703 + -0.5 4,497,703 + +1.4 4,497,703 + +1.1 4,497,703 + -1.9 4,497,703 + +0.2 4,497,704 - -1.3 4,497,704 - -0.2 4,497,704 - -2.0 4,497,704 - -0.5 4,497,704 - +0.2 4,497,704 - -0.0 4,497,704 - -0.8 4,497,704 - -1.3 4,497,704 - +0.6 4,497,704 - -0.5 4,497,704 - +0.0 4,497,704 - -1.0 4,497,704 - -1.8 4,497,704 - -2.3 4,497,704 - +0.6 4,497,704 - +0.8 4,497,708 + -0.4 4,497,739 - -1.4 4,497,739 - -1.1 4,497,739 - +0.6 4,497,745 + +0.5 4,497,745 + -0.3 4,497,745 + +0.5 4,497,745 + +1.0 4,497,745 + +0.2 4,497,745 + +0.3 4,497,745 + +0.0 4,497,746 - +0.0 4,497,746 - -0.2 4,497,746 - -0.1 4,497,746 - -0.6 4,497,746 - -0.4 4,497,746 - -0.3 4,497,746 - -1.3 4,497,746 - +0.7 4,497,805 + +1.0 4,497,805 + +0.4 4,497,806 - +2.8 4,497,810 + -0.2 4,497,810 + -1.8 4,497,810 + -0.1 4,497,810 + -0.5 4,497,811 - +0.3 4,497,811 - -1.8 4,497,828 + +0.5 4,497,828 + -1.5 4,497,828 + +1.0 4,497,828 + -2.0 4,497,829 - +0.2 4,497,829 - +0.2 4,497,831 - +0.3 4,497,840 - +2.7 4,497,848 + +0.4 4,497,848 + -0.1 4,497,849 - -1.0 4,497,849 - -1.0 4,497,849 - +0.7 4,497,866 - -0.5 4,497,866 - -0.3
Or see this region's nucleotide sequence