Experiment: Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS12480 and DZA65_RS12485 are separated by 420 nucleotides DZA65_RS12485 and DZA65_RS12490 overlap by 8 nucleotides DZA65_RS12490 and DZA65_RS12495 are separated by 279 nucleotides
DZA65_RS12480: DZA65_RS12480 - NarK family nitrate/nitrite MFS transporter, at 2,670,605 to 2,671,996
_RS12480
DZA65_RS12485: DZA65_RS12485 - nitrate/nitrite two-component system sensor histidine kinase NarX, at 2,672,417 to 2,674,228
_RS12485
DZA65_RS12490: DZA65_RS12490 - two-component system response regulator NarL, at 2,674,221 to 2,674,868
_RS12490
DZA65_RS12495: DZA65_RS12495 - acyltransferase, at 2,675,148 to 2,676,416
_RS12495
Position (kb)
2672
2673
2674
2675 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2671.445 kb on + strand, within DZA65_RS12480 at 2671.446 kb on - strand, within DZA65_RS12480 at 2671.588 kb on + strand, within DZA65_RS12480 at 2671.588 kb on + strand, within DZA65_RS12480 at 2671.720 kb on - strand, within DZA65_RS12480 at 2671.768 kb on + strand, within DZA65_RS12480 at 2671.768 kb on + strand, within DZA65_RS12480 at 2671.768 kb on + strand, within DZA65_RS12480 at 2671.769 kb on - strand, within DZA65_RS12480 at 2671.769 kb on - strand, within DZA65_RS12480 at 2671.769 kb on - strand, within DZA65_RS12480 at 2671.804 kb on + strand, within DZA65_RS12480 at 2671.805 kb on - strand, within DZA65_RS12480 at 2671.952 kb on - strand at 2671.996 kb on + strand at 2671.996 kb on + strand at 2671.997 kb on - strand at 2671.997 kb on - strand at 2671.997 kb on - strand at 2672.019 kb on + strand at 2672.020 kb on - strand at 2672.043 kb on + strand at 2672.043 kb on + strand at 2672.043 kb on + strand at 2672.043 kb on + strand at 2672.043 kb on + strand at 2672.043 kb on + strand at 2672.043 kb on + strand at 2672.044 kb on - strand at 2672.044 kb on - strand at 2672.044 kb on - strand at 2672.044 kb on - strand at 2672.072 kb on + strand at 2672.072 kb on + strand at 2672.072 kb on + strand at 2672.073 kb on - strand at 2672.127 kb on + strand at 2672.127 kb on + strand at 2672.127 kb on + strand at 2672.132 kb on - strand at 2672.132 kb on - strand at 2672.171 kb on + strand at 2672.172 kb on - strand at 2672.198 kb on + strand at 2672.198 kb on + strand at 2672.325 kb on + strand at 2672.325 kb on + strand at 2672.326 kb on - strand at 2672.347 kb on + strand at 2672.347 kb on + strand at 2672.350 kb on + strand at 2672.350 kb on + strand at 2672.350 kb on + strand at 2672.350 kb on + strand at 2672.350 kb on + strand at 2672.351 kb on - strand at 2672.351 kb on - strand at 2672.351 kb on - strand at 2672.351 kb on - strand at 2672.351 kb on - strand at 2672.351 kb on - strand at 2672.364 kb on + strand at 2672.365 kb on - strand at 2672.366 kb on + strand at 2672.366 kb on + strand at 2672.366 kb on + strand at 2672.366 kb on + strand at 2672.367 kb on - strand at 2672.374 kb on - strand at 2672.589 kb on - strand at 2672.633 kb on + strand, within DZA65_RS12485 at 2672.633 kb on + strand, within DZA65_RS12485 at 2672.633 kb on + strand, within DZA65_RS12485 at 2672.729 kb on + strand, within DZA65_RS12485 at 2672.746 kb on + strand, within DZA65_RS12485 at 2672.746 kb on + strand, within DZA65_RS12485 at 2672.747 kb on - strand, within DZA65_RS12485 at 2672.747 kb on - strand, within DZA65_RS12485 at 2672.862 kb on + strand, within DZA65_RS12485 at 2672.893 kb on + strand, within DZA65_RS12485 at 2672.934 kb on - strand, within DZA65_RS12485 at 2672.965 kb on + strand, within DZA65_RS12485 at 2673.070 kb on + strand, within DZA65_RS12485 at 2673.070 kb on + strand, within DZA65_RS12485 at 2673.070 kb on + strand, within DZA65_RS12485 at 2673.070 kb on + strand at 2673.070 kb on + strand, within DZA65_RS12485 at 2673.070 kb on + strand, within DZA65_RS12485 at 2673.071 kb on - strand, within DZA65_RS12485 at 2673.071 kb on - strand, within DZA65_RS12485 at 2673.071 kb on - strand, within DZA65_RS12485 at 2673.071 kb on - strand, within DZA65_RS12485 at 2673.071 kb on - strand, within DZA65_RS12485 at 2673.071 kb on - strand, within DZA65_RS12485 at 2673.071 kb on - strand, within DZA65_RS12485 at 2673.085 kb on - strand, within DZA65_RS12485 at 2673.085 kb on - strand, within DZA65_RS12485 at 2673.154 kb on + strand, within DZA65_RS12485 at 2673.154 kb on + strand, within DZA65_RS12485 at 2673.231 kb on + strand, within DZA65_RS12485 at 2673.231 kb on + strand, within DZA65_RS12485 at 2673.232 kb on - strand, within DZA65_RS12485 at 2673.232 kb on - strand, within DZA65_RS12485 at 2673.232 kb on - strand, within DZA65_RS12485 at 2673.232 kb on - strand, within DZA65_RS12485 at 2673.232 kb on - strand, within DZA65_RS12485 at 2673.274 kb on - strand, within DZA65_RS12485 at 2673.282 kb on - strand, within DZA65_RS12485 at 2673.282 kb on - strand, within DZA65_RS12485 at 2673.282 kb on - strand, within DZA65_RS12485 at 2673.289 kb on + strand, within DZA65_RS12485 at 2673.290 kb on - strand, within DZA65_RS12485 at 2673.290 kb on - strand, within DZA65_RS12485 at 2673.290 kb on - strand, within DZA65_RS12485 at 2673.417 kb on + strand, within DZA65_RS12485 at 2673.417 kb on + strand, within DZA65_RS12485 at 2673.417 kb on + strand, within DZA65_RS12485 at 2673.418 kb on - strand, within DZA65_RS12485 at 2673.517 kb on + strand, within DZA65_RS12485 at 2673.517 kb on + strand, within DZA65_RS12485 at 2673.518 kb on - strand, within DZA65_RS12485 at 2673.518 kb on - strand, within DZA65_RS12485 at 2673.518 kb on - strand, within DZA65_RS12485 at 2673.518 kb on - strand, within DZA65_RS12485 at 2673.518 kb on - strand, within DZA65_RS12485 at 2673.518 kb on - strand, within DZA65_RS12485 at 2673.604 kb on + strand, within DZA65_RS12485 at 2673.605 kb on - strand, within DZA65_RS12485 at 2673.605 kb on - strand, within DZA65_RS12485 at 2673.605 kb on - strand, within DZA65_RS12485 at 2673.605 kb on - strand, within DZA65_RS12485 at 2673.605 kb on - strand, within DZA65_RS12485 at 2673.605 kb on - strand, within DZA65_RS12485 at 2673.708 kb on + strand, within DZA65_RS12485 at 2673.709 kb on - strand, within DZA65_RS12485 at 2673.709 kb on - strand, within DZA65_RS12485 at 2673.861 kb on - strand, within DZA65_RS12485 at 2673.879 kb on - strand, within DZA65_RS12485 at 2673.879 kb on - strand, within DZA65_RS12485 at 2673.886 kb on - strand, within DZA65_RS12485 at 2673.886 kb on - strand, within DZA65_RS12485 at 2673.886 kb on - strand, within DZA65_RS12485 at 2673.955 kb on + strand, within DZA65_RS12485 at 2673.959 kb on + strand, within DZA65_RS12485 at 2673.959 kb on + strand, within DZA65_RS12485 at 2673.960 kb on - strand, within DZA65_RS12485 at 2673.960 kb on - strand, within DZA65_RS12485 at 2673.960 kb on - strand, within DZA65_RS12485 at 2673.988 kb on + strand at 2673.988 kb on + strand, within DZA65_RS12485 at 2673.988 kb on + strand, within DZA65_RS12485 at 2673.989 kb on - strand, within DZA65_RS12485 at 2673.989 kb on - strand, within DZA65_RS12485 at 2674.015 kb on + strand, within DZA65_RS12485 at 2674.080 kb on - strand at 2674.129 kb on + strand at 2674.129 kb on + strand at 2674.129 kb on + strand at 2674.130 kb on - strand at 2674.130 kb on - strand at 2674.188 kb on - strand at 2674.188 kb on - strand at 2674.295 kb on + strand, within DZA65_RS12490 at 2674.295 kb on + strand, within DZA65_RS12490 at 2674.295 kb on + strand, within DZA65_RS12490 at 2674.295 kb on + strand, within DZA65_RS12490 at 2674.296 kb on - strand, within DZA65_RS12490 at 2674.296 kb on - strand, within DZA65_RS12490 at 2674.296 kb on - strand, within DZA65_RS12490 at 2674.296 kb on - strand, within DZA65_RS12490 at 2674.318 kb on + strand, within DZA65_RS12490 at 2674.514 kb on + strand, within DZA65_RS12490 at 2674.515 kb on - strand, within DZA65_RS12490 at 2674.533 kb on + strand, within DZA65_RS12490 at 2674.533 kb on + strand, within DZA65_RS12490 at 2674.714 kb on + strand, within DZA65_RS12490 at 2674.714 kb on + strand, within DZA65_RS12490 at 2674.714 kb on + strand, within DZA65_RS12490 at 2674.714 kb on + strand, within DZA65_RS12490 at 2674.715 kb on - strand, within DZA65_RS12490 at 2674.715 kb on - strand, within DZA65_RS12490 at 2674.715 kb on - strand, within DZA65_RS12490 at 2674.715 kb on - strand, within DZA65_RS12490 at 2674.715 kb on - strand, within DZA65_RS12490 at 2674.715 kb on - strand, within DZA65_RS12490 at 2674.715 kb on - strand, within DZA65_RS12490 at 2674.715 kb on - strand, within DZA65_RS12490 at 2674.941 kb on - strand at 2674.970 kb on + strand at 2674.988 kb on + strand at 2674.989 kb on - strand at 2674.989 kb on - strand at 2674.990 kb on + strand at 2674.990 kb on + strand at 2674.990 kb on + strand at 2674.991 kb on - strand at 2675.019 kb on + strand at 2675.027 kb on - strand at 2675.039 kb on + strand at 2675.043 kb on - strand at 2675.061 kb on + strand at 2675.065 kb on + strand at 2675.065 kb on + strand at 2675.065 kb on + strand at 2675.065 kb on + strand at 2675.066 kb on - strand at 2675.066 kb on - strand at 2675.168 kb on + strand at 2675.169 kb on - strand at 2675.169 kb on - strand at 2675.205 kb on + strand at 2675.205 kb on + strand at 2675.205 kb on + strand at 2675.205 kb on + strand at 2675.205 kb on + strand at 2675.205 kb on + strand at 2675.205 kb on + strand at 2675.205 kb on + strand at 2675.205 kb on + strand at 2675.206 kb on - strand at 2675.206 kb on - strand at 2675.206 kb on - strand at 2675.206 kb on - strand at 2675.206 kb on - strand at 2675.206 kb on - strand at 2675.206 kb on - strand at 2675.206 kb on - strand at 2675.206 kb on - strand at 2675.206 kb on - strand at 2675.206 kb on - strand at 2675.217 kb on + strand at 2675.217 kb on + strand at 2675.217 kb on + strand at 2675.217 kb on + strand at 2675.218 kb on - strand at 2675.218 kb on - strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.227 kb on + strand at 2675.228 kb on - strand at 2675.228 kb on - strand at 2675.228 kb on - strand at 2675.228 kb on - strand at 2675.228 kb on - strand at 2675.228 kb on - strand at 2675.228 kb on - strand at 2675.228 kb on - strand at 2675.228 kb on - strand at 2675.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Atlantic remove 2,671,445 + DZA65_RS12480 0.60 -0.7 2,671,446 - DZA65_RS12480 0.60 -0.6 2,671,588 + DZA65_RS12480 0.71 -2.1 2,671,588 + DZA65_RS12480 0.71 +1.0 2,671,720 - DZA65_RS12480 0.80 +0.4 2,671,768 + DZA65_RS12480 0.84 -0.5 2,671,768 + DZA65_RS12480 0.84 -0.5 2,671,768 + DZA65_RS12480 0.84 +0.3 2,671,769 - DZA65_RS12480 0.84 +0.3 2,671,769 - DZA65_RS12480 0.84 -0.0 2,671,769 - DZA65_RS12480 0.84 -1.9 2,671,804 + DZA65_RS12480 0.86 -0.7 2,671,805 - DZA65_RS12480 0.86 +1.4 2,671,952 - -0.3 2,671,996 + +0.3 2,671,996 + -2.1 2,671,997 - -0.9 2,671,997 - +0.3 2,671,997 - +0.3 2,672,019 + -1.3 2,672,020 - -0.9 2,672,043 + +1.3 2,672,043 + -0.0 2,672,043 + -0.5 2,672,043 + +1.9 2,672,043 + +0.7 2,672,043 + +0.6 2,672,043 + -0.7 2,672,044 - -0.6 2,672,044 - +0.0 2,672,044 - +0.7 2,672,044 - +0.3 2,672,072 + +0.8 2,672,072 + +0.7 2,672,072 + -0.7 2,672,073 - -0.1 2,672,127 + +0.5 2,672,127 + +0.0 2,672,127 + -0.7 2,672,132 - -0.4 2,672,132 - +1.6 2,672,171 + -0.5 2,672,172 - -0.7 2,672,198 + -0.2 2,672,198 + +0.3 2,672,325 + -0.1 2,672,325 + -0.3 2,672,326 - +0.7 2,672,347 + +1.4 2,672,347 + +1.7 2,672,350 + -1.6 2,672,350 + +0.6 2,672,350 + +0.7 2,672,350 + +1.1 2,672,350 + +1.8 2,672,351 - -1.6 2,672,351 - +0.7 2,672,351 - +0.5 2,672,351 - -1.5 2,672,351 - +0.3 2,672,351 - -2.7 2,672,364 + +0.6 2,672,365 - +0.6 2,672,366 + -0.3 2,672,366 + +1.4 2,672,366 + +0.8 2,672,366 + -1.3 2,672,367 - -0.7 2,672,374 - +1.3 2,672,589 - -0.1 2,672,633 + DZA65_RS12485 0.12 +1.3 2,672,633 + DZA65_RS12485 0.12 -1.1 2,672,633 + DZA65_RS12485 0.12 +1.3 2,672,729 + DZA65_RS12485 0.17 +0.3 2,672,746 + DZA65_RS12485 0.18 -0.2 2,672,746 + DZA65_RS12485 0.18 +0.3 2,672,747 - DZA65_RS12485 0.18 +0.2 2,672,747 - DZA65_RS12485 0.18 +1.4 2,672,862 + DZA65_RS12485 0.25 -0.0 2,672,893 + DZA65_RS12485 0.26 +1.4 2,672,934 - DZA65_RS12485 0.29 -0.1 2,672,965 + DZA65_RS12485 0.30 -0.0 2,673,070 + DZA65_RS12485 0.36 -0.2 2,673,070 + DZA65_RS12485 0.36 +0.7 2,673,070 + DZA65_RS12485 0.36 +1.0 2,673,070 + -0.3 2,673,070 + DZA65_RS12485 0.36 +1.3 2,673,070 + DZA65_RS12485 0.36 -0.3 2,673,071 - DZA65_RS12485 0.36 +1.0 2,673,071 - DZA65_RS12485 0.36 -1.0 2,673,071 - DZA65_RS12485 0.36 -1.5 2,673,071 - DZA65_RS12485 0.36 -1.6 2,673,071 - DZA65_RS12485 0.36 -1.5 2,673,071 - DZA65_RS12485 0.36 +0.4 2,673,071 - DZA65_RS12485 0.36 +1.2 2,673,085 - DZA65_RS12485 0.37 +0.7 2,673,085 - DZA65_RS12485 0.37 +0.4 2,673,154 + DZA65_RS12485 0.41 +0.5 2,673,154 + DZA65_RS12485 0.41 +0.5 2,673,231 + DZA65_RS12485 0.45 +1.7 2,673,231 + DZA65_RS12485 0.45 -0.4 2,673,232 - DZA65_RS12485 0.45 -1.2 2,673,232 - DZA65_RS12485 0.45 -0.6 2,673,232 - DZA65_RS12485 0.45 -0.1 2,673,232 - DZA65_RS12485 0.45 -2.6 2,673,232 - DZA65_RS12485 0.45 +1.6 2,673,274 - DZA65_RS12485 0.47 -0.2 2,673,282 - DZA65_RS12485 0.48 +0.3 2,673,282 - DZA65_RS12485 0.48 -0.7 2,673,282 - DZA65_RS12485 0.48 +0.7 2,673,289 + DZA65_RS12485 0.48 -1.9 2,673,290 - DZA65_RS12485 0.48 +0.3 2,673,290 - DZA65_RS12485 0.48 +0.6 2,673,290 - DZA65_RS12485 0.48 -1.3 2,673,417 + DZA65_RS12485 0.55 -0.2 2,673,417 + DZA65_RS12485 0.55 +0.8 2,673,417 + DZA65_RS12485 0.55 +0.3 2,673,418 - DZA65_RS12485 0.55 -1.2 2,673,517 + DZA65_RS12485 0.61 -0.7 2,673,517 + DZA65_RS12485 0.61 -1.2 2,673,518 - DZA65_RS12485 0.61 -0.0 2,673,518 - DZA65_RS12485 0.61 -0.4 2,673,518 - DZA65_RS12485 0.61 +0.3 2,673,518 - DZA65_RS12485 0.61 +0.7 2,673,518 - DZA65_RS12485 0.61 -0.2 2,673,518 - DZA65_RS12485 0.61 -0.4 2,673,604 + DZA65_RS12485 0.66 -0.5 2,673,605 - DZA65_RS12485 0.66 -1.5 2,673,605 - DZA65_RS12485 0.66 -3.2 2,673,605 - DZA65_RS12485 0.66 +0.0 2,673,605 - DZA65_RS12485 0.66 +1.5 2,673,605 - DZA65_RS12485 0.66 +0.1 2,673,605 - DZA65_RS12485 0.66 +0.3 2,673,708 + DZA65_RS12485 0.71 +0.4 2,673,709 - DZA65_RS12485 0.71 +0.9 2,673,709 - DZA65_RS12485 0.71 -1.9 2,673,861 - DZA65_RS12485 0.80 -0.7 2,673,879 - DZA65_RS12485 0.81 -0.3 2,673,879 - DZA65_RS12485 0.81 +0.9 2,673,886 - DZA65_RS12485 0.81 +0.6 2,673,886 - DZA65_RS12485 0.81 -0.3 2,673,886 - DZA65_RS12485 0.81 -1.3 2,673,955 + DZA65_RS12485 0.85 +0.1 2,673,959 + DZA65_RS12485 0.85 -1.7 2,673,959 + DZA65_RS12485 0.85 +0.9 2,673,960 - DZA65_RS12485 0.85 +2.3 2,673,960 - DZA65_RS12485 0.85 -0.7 2,673,960 - DZA65_RS12485 0.85 -0.6 2,673,988 + -1.7 2,673,988 + DZA65_RS12485 0.87 -1.2 2,673,988 + DZA65_RS12485 0.87 +1.0 2,673,989 - DZA65_RS12485 0.87 -1.6 2,673,989 - DZA65_RS12485 0.87 +0.3 2,674,015 + DZA65_RS12485 0.88 +0.9 2,674,080 - +1.7 2,674,129 + -0.9 2,674,129 + +0.8 2,674,129 + +0.6 2,674,130 - +0.6 2,674,130 - +0.1 2,674,188 - +1.7 2,674,188 - +1.1 2,674,295 + DZA65_RS12490 0.11 -1.2 2,674,295 + DZA65_RS12490 0.11 +1.7 2,674,295 + DZA65_RS12490 0.11 -0.1 2,674,295 + DZA65_RS12490 0.11 -1.2 2,674,296 - DZA65_RS12490 0.12 -0.7 2,674,296 - DZA65_RS12490 0.12 -1.7 2,674,296 - DZA65_RS12490 0.12 -0.7 2,674,296 - DZA65_RS12490 0.12 +1.0 2,674,318 + DZA65_RS12490 0.15 +0.6 2,674,514 + DZA65_RS12490 0.45 +0.5 2,674,515 - DZA65_RS12490 0.45 +1.3 2,674,533 + DZA65_RS12490 0.48 +0.3 2,674,533 + DZA65_RS12490 0.48 -0.1 2,674,714 + DZA65_RS12490 0.76 -0.3 2,674,714 + DZA65_RS12490 0.76 +0.3 2,674,714 + DZA65_RS12490 0.76 +1.6 2,674,714 + DZA65_RS12490 0.76 +2.3 2,674,715 - DZA65_RS12490 0.76 -0.2 2,674,715 - DZA65_RS12490 0.76 +1.1 2,674,715 - DZA65_RS12490 0.76 +0.6 2,674,715 - DZA65_RS12490 0.76 +0.7 2,674,715 - DZA65_RS12490 0.76 -1.4 2,674,715 - DZA65_RS12490 0.76 -0.3 2,674,715 - DZA65_RS12490 0.76 +0.1 2,674,715 - DZA65_RS12490 0.76 +0.7 2,674,941 - -0.3 2,674,970 + -0.6 2,674,988 + -0.7 2,674,989 - -1.5 2,674,989 - -1.2 2,674,990 + -1.1 2,674,990 + -0.0 2,674,990 + +0.8 2,674,991 - -0.5 2,675,019 + -0.6 2,675,027 - +0.1 2,675,039 + -0.2 2,675,043 - -0.1 2,675,061 + -0.6 2,675,065 + +0.1 2,675,065 + -0.2 2,675,065 + +0.9 2,675,065 + +0.6 2,675,066 - -0.7 2,675,066 - -1.6 2,675,168 + -1.4 2,675,169 - +0.5 2,675,169 - -1.0 2,675,205 + +0.3 2,675,205 + -1.7 2,675,205 + +1.1 2,675,205 + +0.4 2,675,205 + +0.0 2,675,205 + +0.5 2,675,205 + +1.7 2,675,205 + -0.2 2,675,205 + +2.4 2,675,206 - -3.1 2,675,206 - -0.6 2,675,206 - +0.8 2,675,206 - -0.9 2,675,206 - +1.1 2,675,206 - +1.3 2,675,206 - +1.3 2,675,206 - -0.4 2,675,206 - -0.1 2,675,206 - -0.9 2,675,206 - -1.9 2,675,217 + -0.7 2,675,217 + -1.0 2,675,217 + +0.1 2,675,217 + +0.8 2,675,218 - -2.1 2,675,218 - +1.3 2,675,227 + +1.1 2,675,227 + -0.3 2,675,227 + -0.0 2,675,227 + -0.0 2,675,227 + -1.7 2,675,227 + -1.4 2,675,227 + -0.1 2,675,227 + +2.1 2,675,227 + -0.1 2,675,227 + -0.1 2,675,227 + -0.1 2,675,227 + -2.1 2,675,227 + -0.4 2,675,227 + +1.0 2,675,227 + -1.4 2,675,228 - +0.3 2,675,228 - -0.2 2,675,228 - -1.6 2,675,228 - -0.7 2,675,228 - +0.0 2,675,228 - +1.2 2,675,228 - -0.7 2,675,228 - -1.2 2,675,228 - -2.2 2,675,228 - +1.6
Or see this region's nucleotide sequence