Experiment: Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS21080 and DZA65_RS21085 are separated by 86 nucleotides DZA65_RS21085 and DZA65_RS21090 overlap by 1 nucleotides DZA65_RS21090 and DZA65_RS21095 overlap by 4 nucleotides DZA65_RS21095 and DZA65_RS21100 overlap by 4 nucleotides
DZA65_RS21080: DZA65_RS21080 - DNA helicase II, at 4,556,984 to 4,559,146
_RS21080
DZA65_RS21085: DZA65_RS21085 - 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YigB, at 4,559,233 to 4,559,949
_RS21085
DZA65_RS21090: DZA65_RS21090 - tyrosine recombinase XerC, at 4,559,949 to 4,560,857
_RS21090
DZA65_RS21095: DZA65_RS21095 - DUF484 domain-containing protein, at 4,560,854 to 4,561,555
_RS21095
DZA65_RS21100: DZA65_RS21100 - diaminopimelate epimerase, at 4,561,552 to 4,562,376
_RS21100
Position (kb) 4559
4560
4561 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4559.066 kb on + strand at 4559.110 kb on + strand at 4559.138 kb on + strand at 4559.138 kb on + strand at 4559.138 kb on + strand at 4559.138 kb on + strand at 4559.139 kb on - strand at 4559.139 kb on - strand at 4559.139 kb on - strand at 4559.146 kb on + strand at 4559.147 kb on - strand at 4559.234 kb on + strand at 4559.234 kb on + strand at 4559.234 kb on + strand at 4559.234 kb on + strand at 4559.234 kb on + strand at 4559.235 kb on - strand at 4559.235 kb on - strand at 4559.236 kb on + strand at 4559.236 kb on + strand at 4559.237 kb on - strand at 4559.328 kb on + strand, within DZA65_RS21085 at 4559.329 kb on - strand, within DZA65_RS21085 at 4559.344 kb on + strand, within DZA65_RS21085 at 4559.345 kb on - strand at 4559.345 kb on - strand, within DZA65_RS21085 at 4559.345 kb on - strand, within DZA65_RS21085 at 4559.358 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.444 kb on + strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.445 kb on - strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.448 kb on + strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.449 kb on - strand, within DZA65_RS21085 at 4559.493 kb on + strand, within DZA65_RS21085 at 4559.494 kb on - strand, within DZA65_RS21085 at 4559.504 kb on + strand, within DZA65_RS21085 at 4559.505 kb on - strand, within DZA65_RS21085 at 4559.710 kb on + strand, within DZA65_RS21085 at 4559.710 kb on + strand, within DZA65_RS21085 at 4559.710 kb on + strand, within DZA65_RS21085 at 4559.710 kb on + strand, within DZA65_RS21085 at 4559.711 kb on - strand, within DZA65_RS21085 at 4559.711 kb on - strand, within DZA65_RS21085 at 4559.711 kb on - strand, within DZA65_RS21085 at 4559.711 kb on - strand, within DZA65_RS21085 at 4559.711 kb on - strand, within DZA65_RS21085 at 4559.744 kb on + strand, within DZA65_RS21085 at 4559.744 kb on + strand, within DZA65_RS21085 at 4559.745 kb on - strand, within DZA65_RS21085 at 4559.825 kb on + strand, within DZA65_RS21085 at 4559.825 kb on + strand, within DZA65_RS21085 at 4559.826 kb on - strand, within DZA65_RS21085 at 4559.876 kb on + strand, within DZA65_RS21085 at 4559.876 kb on + strand, within DZA65_RS21085 at 4559.876 kb on + strand, within DZA65_RS21085 at 4559.876 kb on + strand, within DZA65_RS21085 at 4559.876 kb on + strand, within DZA65_RS21085 at 4559.876 kb on + strand, within DZA65_RS21085 at 4559.876 kb on + strand, within DZA65_RS21085 at 4559.876 kb on + strand, within DZA65_RS21085 at 4559.876 kb on + strand, within DZA65_RS21085 at 4559.876 kb on + strand, within DZA65_RS21085 at 4559.877 kb on - strand, within DZA65_RS21085 at 4559.877 kb on - strand, within DZA65_RS21085 at 4559.877 kb on - strand, within DZA65_RS21085 at 4559.877 kb on - strand, within DZA65_RS21085 at 4559.877 kb on - strand, within DZA65_RS21085 at 4559.877 kb on - strand, within DZA65_RS21085 at 4559.877 kb on - strand, within DZA65_RS21085 at 4559.877 kb on - strand, within DZA65_RS21085 at 4559.877 kb on - strand, within DZA65_RS21085 at 4559.877 kb on - strand, within DZA65_RS21085 at 4559.881 kb on - strand at 4559.881 kb on - strand at 4559.881 kb on - strand at 4559.881 kb on - strand at 4559.886 kb on - strand at 4559.886 kb on - strand at 4559.886 kb on - strand at 4559.945 kb on + strand at 4559.945 kb on + strand at 4559.945 kb on + strand at 4559.945 kb on + strand at 4559.946 kb on - strand at 4559.946 kb on - strand at 4559.946 kb on - strand at 4559.946 kb on - strand at 4559.946 kb on - strand at 4559.957 kb on + strand at 4559.958 kb on - strand at 4559.990 kb on - strand at 4560.048 kb on - strand at 4560.129 kb on - strand, within DZA65_RS21090 at 4560.190 kb on - strand, within DZA65_RS21090 at 4560.326 kb on + strand, within DZA65_RS21090 at 4560.763 kb on + strand, within DZA65_RS21090 at 4560.857 kb on + strand at 4560.858 kb on - strand at 4561.077 kb on + strand, within DZA65_RS21095 at 4561.077 kb on + strand, within DZA65_RS21095 at 4561.077 kb on + strand, within DZA65_RS21095 at 4561.078 kb on - strand, within DZA65_RS21095 at 4561.078 kb on - strand, within DZA65_RS21095 at 4561.078 kb on - strand, within DZA65_RS21095 at 4561.078 kb on - strand, within DZA65_RS21095 at 4561.078 kb on - strand, within DZA65_RS21095 at 4561.127 kb on - strand, within DZA65_RS21095 at 4561.147 kb on + strand, within DZA65_RS21095 at 4561.148 kb on - strand, within DZA65_RS21095 at 4561.270 kb on + strand, within DZA65_RS21095 at 4561.295 kb on + strand, within DZA65_RS21095 at 4561.295 kb on + strand, within DZA65_RS21095 at 4561.295 kb on + strand, within DZA65_RS21095 at 4561.296 kb on - strand, within DZA65_RS21095 at 4561.296 kb on - strand, within DZA65_RS21095 at 4561.296 kb on - strand, within DZA65_RS21095 at 4561.346 kb on + strand, within DZA65_RS21095 at 4561.347 kb on - strand, within DZA65_RS21095 at 4561.347 kb on - strand, within DZA65_RS21095 at 4561.347 kb on - strand, within DZA65_RS21095 at 4561.363 kb on - strand, within DZA65_RS21095 at 4561.435 kb on + strand, within DZA65_RS21095 at 4561.436 kb on - strand, within DZA65_RS21095 at 4561.436 kb on - strand, within DZA65_RS21095 at 4561.495 kb on - strand at 4561.555 kb on + strand at 4561.555 kb on + strand at 4561.555 kb on + strand at 4561.556 kb on - strand at 4561.556 kb on - strand at 4561.556 kb on - strand at 4561.556 kb on - strand at 4561.556 kb on - strand at 4561.564 kb on - strand at 4561.564 kb on - strand at 4561.564 kb on - strand at 4561.564 kb on - strand at 4561.564 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Atlantic remove 4,559,066 + +0.4 4,559,110 + +1.2 4,559,138 + +0.6 4,559,138 + -0.1 4,559,138 + -0.3 4,559,138 + -0.4 4,559,139 - +0.7 4,559,139 - -1.1 4,559,139 - -0.6 4,559,146 + -0.1 4,559,147 - -1.4 4,559,234 + -0.4 4,559,234 + +0.1 4,559,234 + +1.3 4,559,234 + +0.6 4,559,234 + -0.1 4,559,235 - -0.1 4,559,235 - +0.6 4,559,236 + -0.1 4,559,236 + -0.1 4,559,237 - -1.9 4,559,328 + DZA65_RS21085 0.13 -1.7 4,559,329 - DZA65_RS21085 0.13 -0.7 4,559,344 + DZA65_RS21085 0.15 -0.1 4,559,345 - +2.1 4,559,345 - DZA65_RS21085 0.16 +0.6 4,559,345 - DZA65_RS21085 0.16 +0.6 4,559,358 + DZA65_RS21085 0.17 +0.5 4,559,444 + DZA65_RS21085 0.29 +0.5 4,559,444 + DZA65_RS21085 0.29 +1.6 4,559,444 + DZA65_RS21085 0.29 -0.7 4,559,444 + DZA65_RS21085 0.29 -1.1 4,559,444 + DZA65_RS21085 0.29 +0.2 4,559,444 + DZA65_RS21085 0.29 +1.6 4,559,444 + DZA65_RS21085 0.29 +1.2 4,559,444 + DZA65_RS21085 0.29 +0.1 4,559,444 + DZA65_RS21085 0.29 -0.3 4,559,444 + DZA65_RS21085 0.29 -0.1 4,559,444 + DZA65_RS21085 0.29 -1.0 4,559,444 + DZA65_RS21085 0.29 -1.1 4,559,444 + DZA65_RS21085 0.29 -1.3 4,559,444 + DZA65_RS21085 0.29 +0.2 4,559,444 + DZA65_RS21085 0.29 +1.3 4,559,444 + DZA65_RS21085 0.29 +0.8 4,559,444 + DZA65_RS21085 0.29 +0.8 4,559,444 + DZA65_RS21085 0.29 -0.7 4,559,444 + DZA65_RS21085 0.29 +0.0 4,559,444 + DZA65_RS21085 0.29 +1.2 4,559,445 - DZA65_RS21085 0.30 -0.5 4,559,445 - +2.5 4,559,445 - DZA65_RS21085 0.30 +0.1 4,559,445 - DZA65_RS21085 0.30 +0.6 4,559,445 - DZA65_RS21085 0.30 +1.2 4,559,445 - DZA65_RS21085 0.30 +1.5 4,559,445 - DZA65_RS21085 0.30 +1.7 4,559,445 - DZA65_RS21085 0.30 +0.9 4,559,445 - DZA65_RS21085 0.30 +1.8 4,559,445 - DZA65_RS21085 0.30 -1.0 4,559,445 - DZA65_RS21085 0.30 +0.8 4,559,445 - DZA65_RS21085 0.30 -0.7 4,559,445 - DZA65_RS21085 0.30 +1.7 4,559,445 - DZA65_RS21085 0.30 +0.3 4,559,448 + DZA65_RS21085 0.30 -1.7 4,559,448 + DZA65_RS21085 0.30 +1.8 4,559,448 + DZA65_RS21085 0.30 -0.9 4,559,448 + DZA65_RS21085 0.30 -0.5 4,559,448 + DZA65_RS21085 0.30 +1.3 4,559,448 + DZA65_RS21085 0.30 -0.7 4,559,448 + DZA65_RS21085 0.30 -0.4 4,559,448 + DZA65_RS21085 0.30 -0.2 4,559,448 + DZA65_RS21085 0.30 +0.1 4,559,448 + DZA65_RS21085 0.30 +0.0 4,559,448 + DZA65_RS21085 0.30 +0.5 4,559,448 + DZA65_RS21085 0.30 +0.5 4,559,448 + DZA65_RS21085 0.30 -0.0 4,559,448 + DZA65_RS21085 0.30 +0.3 4,559,448 + DZA65_RS21085 0.30 -0.5 4,559,448 + DZA65_RS21085 0.30 -1.1 4,559,448 + DZA65_RS21085 0.30 -0.4 4,559,449 - DZA65_RS21085 0.30 -0.1 4,559,449 - DZA65_RS21085 0.30 -0.1 4,559,449 - DZA65_RS21085 0.30 -2.4 4,559,449 - DZA65_RS21085 0.30 -0.7 4,559,449 - DZA65_RS21085 0.30 -0.3 4,559,449 - DZA65_RS21085 0.30 -0.5 4,559,449 - DZA65_RS21085 0.30 -0.3 4,559,449 - DZA65_RS21085 0.30 -0.0 4,559,449 - DZA65_RS21085 0.30 -0.1 4,559,449 - DZA65_RS21085 0.30 +1.0 4,559,449 - DZA65_RS21085 0.30 -0.2 4,559,449 - DZA65_RS21085 0.30 -1.9 4,559,449 - DZA65_RS21085 0.30 -0.4 4,559,449 - DZA65_RS21085 0.30 -0.4 4,559,449 - DZA65_RS21085 0.30 -0.3 4,559,449 - DZA65_RS21085 0.30 -0.9 4,559,449 - DZA65_RS21085 0.30 -0.9 4,559,449 - DZA65_RS21085 0.30 -0.5 4,559,449 - DZA65_RS21085 0.30 +0.2 4,559,449 - DZA65_RS21085 0.30 -0.9 4,559,449 - DZA65_RS21085 0.30 -0.1 4,559,449 - DZA65_RS21085 0.30 +0.3 4,559,449 - DZA65_RS21085 0.30 -1.2 4,559,449 - DZA65_RS21085 0.30 -1.4 4,559,449 - DZA65_RS21085 0.30 -0.9 4,559,449 - DZA65_RS21085 0.30 +1.5 4,559,449 - DZA65_RS21085 0.30 +3.6 4,559,449 - DZA65_RS21085 0.30 +0.4 4,559,449 - DZA65_RS21085 0.30 -0.1 4,559,449 - DZA65_RS21085 0.30 +1.1 4,559,449 - DZA65_RS21085 0.30 -0.7 4,559,449 - +3.2 4,559,449 - DZA65_RS21085 0.30 -0.7 4,559,449 - DZA65_RS21085 0.30 +0.2 4,559,493 + DZA65_RS21085 0.36 -0.0 4,559,494 - DZA65_RS21085 0.36 -0.9 4,559,504 + DZA65_RS21085 0.38 -0.3 4,559,505 - DZA65_RS21085 0.38 +1.6 4,559,710 + DZA65_RS21085 0.67 -1.4 4,559,710 + DZA65_RS21085 0.67 +1.9 4,559,710 + DZA65_RS21085 0.67 -0.9 4,559,710 + DZA65_RS21085 0.67 +0.5 4,559,711 - DZA65_RS21085 0.67 +1.6 4,559,711 - DZA65_RS21085 0.67 -2.0 4,559,711 - DZA65_RS21085 0.67 +0.3 4,559,711 - DZA65_RS21085 0.67 -2.1 4,559,711 - DZA65_RS21085 0.67 -0.4 4,559,744 + DZA65_RS21085 0.71 -1.1 4,559,744 + DZA65_RS21085 0.71 +0.4 4,559,745 - DZA65_RS21085 0.71 +0.9 4,559,825 + DZA65_RS21085 0.83 -1.4 4,559,825 + DZA65_RS21085 0.83 -1.1 4,559,826 - DZA65_RS21085 0.83 -0.6 4,559,876 + DZA65_RS21085 0.90 +3.6 4,559,876 + DZA65_RS21085 0.90 -1.4 4,559,876 + DZA65_RS21085 0.90 +0.0 4,559,876 + DZA65_RS21085 0.90 -0.3 4,559,876 + DZA65_RS21085 0.90 +0.6 4,559,876 + DZA65_RS21085 0.90 -0.4 4,559,876 + DZA65_RS21085 0.90 +1.3 4,559,876 + DZA65_RS21085 0.90 -1.3 4,559,876 + DZA65_RS21085 0.90 -0.0 4,559,876 + DZA65_RS21085 0.90 -1.9 4,559,877 - DZA65_RS21085 0.90 +0.9 4,559,877 - DZA65_RS21085 0.90 +0.6 4,559,877 - DZA65_RS21085 0.90 +1.5 4,559,877 - DZA65_RS21085 0.90 -0.9 4,559,877 - DZA65_RS21085 0.90 +0.7 4,559,877 - DZA65_RS21085 0.90 +1.0 4,559,877 - DZA65_RS21085 0.90 -0.5 4,559,877 - DZA65_RS21085 0.90 +0.1 4,559,877 - DZA65_RS21085 0.90 +0.6 4,559,877 - DZA65_RS21085 0.90 -0.1 4,559,881 - +1.9 4,559,881 - -1.9 4,559,881 - -0.1 4,559,881 - -0.1 4,559,886 - +0.1 4,559,886 - -0.7 4,559,886 - -2.3 4,559,945 + -0.4 4,559,945 + -1.3 4,559,945 + +0.3 4,559,945 + -0.3 4,559,946 - -0.7 4,559,946 - +2.2 4,559,946 - -0.1 4,559,946 - -3.2 4,559,946 - +0.3 4,559,957 + +0.0 4,559,958 - +1.0 4,559,990 - -0.1 4,560,048 - +0.9 4,560,129 - DZA65_RS21090 0.20 +0.3 4,560,190 - DZA65_RS21090 0.27 -0.9 4,560,326 + DZA65_RS21090 0.41 -0.1 4,560,763 + DZA65_RS21090 0.90 -2.1 4,560,857 + -0.7 4,560,858 - -0.4 4,561,077 + DZA65_RS21095 0.32 -2.1 4,561,077 + DZA65_RS21095 0.32 -0.6 4,561,077 + DZA65_RS21095 0.32 -1.0 4,561,078 - DZA65_RS21095 0.32 +0.5 4,561,078 - DZA65_RS21095 0.32 -0.1 4,561,078 - DZA65_RS21095 0.32 +0.3 4,561,078 - DZA65_RS21095 0.32 +1.4 4,561,078 - DZA65_RS21095 0.32 +1.9 4,561,127 - DZA65_RS21095 0.39 +1.2 4,561,147 + DZA65_RS21095 0.42 -0.1 4,561,148 - DZA65_RS21095 0.42 +0.3 4,561,270 + DZA65_RS21095 0.59 -1.4 4,561,295 + DZA65_RS21095 0.63 +0.7 4,561,295 + DZA65_RS21095 0.63 +1.6 4,561,295 + DZA65_RS21095 0.63 -1.3 4,561,296 - DZA65_RS21095 0.63 +0.4 4,561,296 - DZA65_RS21095 0.63 +0.5 4,561,296 - DZA65_RS21095 0.63 -1.4 4,561,346 + DZA65_RS21095 0.70 -1.2 4,561,347 - DZA65_RS21095 0.70 +0.6 4,561,347 - DZA65_RS21095 0.70 -1.8 4,561,347 - DZA65_RS21095 0.70 +0.2 4,561,363 - DZA65_RS21095 0.73 -0.3 4,561,435 + DZA65_RS21095 0.83 +0.4 4,561,436 - DZA65_RS21095 0.83 +1.5 4,561,436 - DZA65_RS21095 0.83 +0.3 4,561,495 - +0.8 4,561,555 + -0.2 4,561,555 + +1.3 4,561,555 + -2.3 4,561,556 - -0.1 4,561,556 - -0.4 4,561,556 - -0.4 4,561,556 - -0.2 4,561,556 - -0.1 4,561,564 - -0.7 4,561,564 - -0.8 4,561,564 - +0.9 4,561,564 - -1.4 4,561,564 - -0.5
Or see this region's nucleotide sequence