Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS08135 and DZA65_RS08140 are separated by 74 nucleotides DZA65_RS08140 and DZA65_RS08145 are separated by 3 nucleotides DZA65_RS08145 and DZA65_RS08150 are separated by 89 nucleotides DZA65_RS08150 and DZA65_RS08155 overlap by 4 nucleotides DZA65_RS08155 and DZA65_RS08160 are separated by 73 nucleotides DZA65_RS08160 and DZA65_RS08165 are separated by 280 nucleotides
DZA65_RS08135: DZA65_RS08135 - RraA family protein, at 1,703,415 to 1,704,032
_RS08135
DZA65_RS08140: DZA65_RS08140 - type II toxin-antitoxin system HicB family antitoxin, at 1,704,107 to 1,704,517
_RS08140
DZA65_RS08145: DZA65_RS08145 - type II toxin-antitoxin system HicA family toxin, at 1,704,521 to 1,704,673
_RS08145
DZA65_RS08150: DZA65_RS08150 - hypothetical protein, at 1,704,763 to 1,705,641
_RS08150
DZA65_RS08155: DZA65_RS08155 - helix-turn-helix transcriptional regulator, at 1,705,638 to 1,706,006
_RS08155
DZA65_RS08160: DZA65_RS08160 - type II toxin-antitoxin system RelE/ParE family toxin, at 1,706,080 to 1,706,340
_RS08160
DZA65_RS08165: DZA65_RS08165 - hydrolase, at 1,706,621 to 1,707,676
_RS08165
Position (kb)
1704
1705
1706 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1703.927 kb on + strand, within DZA65_RS08135 at 1704.030 kb on + strand at 1704.030 kb on + strand at 1704.030 kb on + strand at 1704.030 kb on + strand at 1704.030 kb on + strand at 1704.030 kb on + strand at 1704.031 kb on - strand at 1704.031 kb on - strand at 1704.101 kb on + strand at 1704.102 kb on - strand at 1704.167 kb on - strand, within DZA65_RS08140 at 1704.232 kb on + strand, within DZA65_RS08140 at 1704.291 kb on + strand, within DZA65_RS08140 at 1704.300 kb on + strand, within DZA65_RS08140 at 1704.347 kb on - strand, within DZA65_RS08140 at 1704.347 kb on - strand, within DZA65_RS08140 at 1704.471 kb on + strand, within DZA65_RS08140 at 1704.472 kb on - strand, within DZA65_RS08140 at 1704.482 kb on - strand at 1704.511 kb on - strand at 1704.517 kb on + strand at 1704.517 kb on + strand at 1704.518 kb on - strand at 1704.518 kb on - strand at 1704.585 kb on - strand, within DZA65_RS08145 at 1704.591 kb on - strand, within DZA65_RS08145 at 1704.602 kb on - strand, within DZA65_RS08145 at 1704.620 kb on + strand, within DZA65_RS08145 at 1704.644 kb on - strand, within DZA65_RS08145 at 1704.644 kb on - strand, within DZA65_RS08145 at 1704.696 kb on + strand at 1704.697 kb on - strand at 1704.793 kb on - strand at 1704.862 kb on + strand, within DZA65_RS08150 at 1704.901 kb on - strand, within DZA65_RS08150 at 1704.920 kb on + strand, within DZA65_RS08150 at 1704.920 kb on + strand, within DZA65_RS08150 at 1704.921 kb on - strand, within DZA65_RS08150 at 1704.940 kb on + strand, within DZA65_RS08150 at 1704.999 kb on + strand, within DZA65_RS08150 at 1705.004 kb on - strand, within DZA65_RS08150 at 1705.017 kb on + strand, within DZA65_RS08150 at 1705.021 kb on - strand, within DZA65_RS08150 at 1705.021 kb on - strand, within DZA65_RS08150 at 1705.034 kb on - strand, within DZA65_RS08150 at 1705.051 kb on - strand, within DZA65_RS08150 at 1705.098 kb on + strand, within DZA65_RS08150 at 1705.099 kb on - strand, within DZA65_RS08150 at 1705.099 kb on - strand, within DZA65_RS08150 at 1705.122 kb on + strand, within DZA65_RS08150 at 1705.123 kb on - strand, within DZA65_RS08150 at 1705.129 kb on + strand, within DZA65_RS08150 at 1705.129 kb on + strand, within DZA65_RS08150 at 1705.135 kb on + strand, within DZA65_RS08150 at 1705.135 kb on + strand, within DZA65_RS08150 at 1705.136 kb on - strand, within DZA65_RS08150 at 1705.178 kb on + strand, within DZA65_RS08150 at 1705.207 kb on + strand, within DZA65_RS08150 at 1705.228 kb on + strand, within DZA65_RS08150 at 1705.330 kb on + strand, within DZA65_RS08150 at 1705.343 kb on + strand, within DZA65_RS08150 at 1705.348 kb on + strand, within DZA65_RS08150 at 1705.348 kb on + strand, within DZA65_RS08150 at 1705.423 kb on - strand, within DZA65_RS08150 at 1705.424 kb on + strand, within DZA65_RS08150 at 1705.424 kb on + strand, within DZA65_RS08150 at 1705.425 kb on - strand, within DZA65_RS08150 at 1705.448 kb on - strand, within DZA65_RS08150 at 1705.448 kb on - strand, within DZA65_RS08150 at 1705.450 kb on - strand, within DZA65_RS08150 at 1705.477 kb on - strand, within DZA65_RS08150 at 1705.485 kb on - strand, within DZA65_RS08150 at 1705.488 kb on + strand, within DZA65_RS08150 at 1705.488 kb on + strand, within DZA65_RS08150 at 1705.527 kb on - strand, within DZA65_RS08150 at 1705.554 kb on - strand at 1705.604 kb on - strand at 1705.604 kb on - strand at 1705.635 kb on - strand at 1705.635 kb on - strand at 1705.635 kb on - strand at 1706.026 kb on - strand at 1706.044 kb on - strand at 1706.044 kb on - strand at 1706.085 kb on - strand at 1706.142 kb on + strand, within DZA65_RS08160 at 1706.142 kb on + strand, within DZA65_RS08160 at 1706.166 kb on - strand, within DZA65_RS08160 at 1706.190 kb on + strand, within DZA65_RS08160 at 1706.191 kb on - strand, within DZA65_RS08160 at 1706.191 kb on - strand, within DZA65_RS08160 at 1706.191 kb on - strand, within DZA65_RS08160 at 1706.267 kb on - strand, within DZA65_RS08160 at 1706.279 kb on + strand, within DZA65_RS08160 at 1706.336 kb on - strand at 1706.336 kb on - strand at 1706.336 kb on - strand at 1706.366 kb on + strand at 1706.367 kb on - strand at 1706.378 kb on + strand at 1706.403 kb on - strand at 1706.424 kb on + strand at 1706.424 kb on + strand at 1706.424 kb on + strand at 1706.443 kb on + strand at 1706.443 kb on + strand at 1706.443 kb on + strand at 1706.444 kb on - strand at 1706.444 kb on - strand at 1706.444 kb on - strand at 1706.444 kb on - strand at 1706.444 kb on - strand at 1706.444 kb on - strand at 1706.444 kb on - strand at 1706.444 kb on - strand at 1706.451 kb on + strand at 1706.451 kb on + strand at 1706.452 kb on - strand at 1706.514 kb on + strand at 1706.522 kb on - strand at 1706.548 kb on + strand at 1706.559 kb on + strand at 1706.591 kb on + strand at 1706.591 kb on + strand at 1706.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 1,703,927 + DZA65_RS08135 0.83 -1.8 1,704,030 + -0.6 1,704,030 + -0.4 1,704,030 + +0.8 1,704,030 + -0.5 1,704,030 + -1.6 1,704,030 + +0.2 1,704,031 - +0.6 1,704,031 - +0.5 1,704,101 + -0.8 1,704,102 - +2.2 1,704,167 - DZA65_RS08140 0.15 -1.8 1,704,232 + DZA65_RS08140 0.30 +0.8 1,704,291 + DZA65_RS08140 0.45 -1.3 1,704,300 + DZA65_RS08140 0.47 -2.8 1,704,347 - DZA65_RS08140 0.58 +1.9 1,704,347 - DZA65_RS08140 0.58 -1.8 1,704,471 + DZA65_RS08140 0.89 -0.1 1,704,472 - DZA65_RS08140 0.89 -1.8 1,704,482 - -0.0 1,704,511 - +1.5 1,704,517 + -0.8 1,704,517 + -0.1 1,704,518 - -0.4 1,704,518 - -0.5 1,704,585 - DZA65_RS08145 0.42 -1.9 1,704,591 - DZA65_RS08145 0.46 -0.5 1,704,602 - DZA65_RS08145 0.53 +0.8 1,704,620 + DZA65_RS08145 0.65 +1.5 1,704,644 - DZA65_RS08145 0.80 -1.3 1,704,644 - DZA65_RS08145 0.80 +0.2 1,704,696 + +0.2 1,704,697 - -1.8 1,704,793 - -1.2 1,704,862 + DZA65_RS08150 0.11 +0.1 1,704,901 - DZA65_RS08150 0.16 +0.2 1,704,920 + DZA65_RS08150 0.18 -0.2 1,704,920 + DZA65_RS08150 0.18 -0.2 1,704,921 - DZA65_RS08150 0.18 -2.6 1,704,940 + DZA65_RS08150 0.20 -0.4 1,704,999 + DZA65_RS08150 0.27 -1.1 1,705,004 - DZA65_RS08150 0.27 -2.6 1,705,017 + DZA65_RS08150 0.29 -0.8 1,705,021 - DZA65_RS08150 0.29 +0.1 1,705,021 - DZA65_RS08150 0.29 +0.4 1,705,034 - DZA65_RS08150 0.31 -0.5 1,705,051 - DZA65_RS08150 0.33 -2.3 1,705,098 + DZA65_RS08150 0.38 -0.8 1,705,099 - DZA65_RS08150 0.38 +0.5 1,705,099 - DZA65_RS08150 0.38 -0.9 1,705,122 + DZA65_RS08150 0.41 -0.2 1,705,123 - DZA65_RS08150 0.41 +0.9 1,705,129 + DZA65_RS08150 0.42 +0.5 1,705,129 + DZA65_RS08150 0.42 -0.8 1,705,135 + DZA65_RS08150 0.42 +0.2 1,705,135 + DZA65_RS08150 0.42 +0.1 1,705,136 - DZA65_RS08150 0.42 -0.4 1,705,178 + DZA65_RS08150 0.47 +1.2 1,705,207 + DZA65_RS08150 0.51 -0.3 1,705,228 + DZA65_RS08150 0.53 -0.5 1,705,330 + DZA65_RS08150 0.65 +0.2 1,705,343 + DZA65_RS08150 0.66 +0.2 1,705,348 + DZA65_RS08150 0.67 +0.2 1,705,348 + DZA65_RS08150 0.67 +0.4 1,705,423 - DZA65_RS08150 0.75 -3.1 1,705,424 + DZA65_RS08150 0.75 -1.2 1,705,424 + DZA65_RS08150 0.75 -1.1 1,705,425 - DZA65_RS08150 0.75 -0.1 1,705,448 - DZA65_RS08150 0.78 +0.4 1,705,448 - DZA65_RS08150 0.78 +0.2 1,705,450 - DZA65_RS08150 0.78 -1.3 1,705,477 - DZA65_RS08150 0.81 -0.3 1,705,485 - DZA65_RS08150 0.82 -0.1 1,705,488 + DZA65_RS08150 0.82 -0.6 1,705,488 + DZA65_RS08150 0.82 +0.2 1,705,527 - DZA65_RS08150 0.87 +1.6 1,705,554 - +1.7 1,705,604 - -1.3 1,705,604 - -1.1 1,705,635 - +1.2 1,705,635 - +0.9 1,705,635 - -2.8 1,706,026 - -0.9 1,706,044 - -0.7 1,706,044 - +0.8 1,706,085 - +0.2 1,706,142 + DZA65_RS08160 0.24 -0.5 1,706,142 + DZA65_RS08160 0.24 -0.9 1,706,166 - DZA65_RS08160 0.33 -0.9 1,706,190 + DZA65_RS08160 0.42 -0.6 1,706,191 - DZA65_RS08160 0.43 -2.5 1,706,191 - DZA65_RS08160 0.43 -0.1 1,706,191 - DZA65_RS08160 0.43 -1.0 1,706,267 - DZA65_RS08160 0.72 +0.0 1,706,279 + DZA65_RS08160 0.76 +0.1 1,706,336 - -0.3 1,706,336 - -0.2 1,706,336 - +0.7 1,706,366 + -2.8 1,706,367 - -2.1 1,706,378 + +0.2 1,706,403 - -0.8 1,706,424 + +0.2 1,706,424 + -0.8 1,706,424 + +0.3 1,706,443 + -0.6 1,706,443 + -0.9 1,706,443 + -0.4 1,706,444 - -1.0 1,706,444 - +0.2 1,706,444 - +0.5 1,706,444 - -1.1 1,706,444 - +0.7 1,706,444 - +0.2 1,706,444 - +2.0 1,706,444 - -1.8 1,706,451 + +0.9 1,706,451 + +0.4 1,706,452 - -1.0 1,706,514 + -1.5 1,706,522 - +0.1 1,706,548 + -0.4 1,706,559 + +0.1 1,706,591 + +0.1 1,706,591 + -0.2 1,706,592 - -1.8
Or see this region's nucleotide sequence