Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS14315 and DDA3937_RS14320 overlap by 4 nucleotides DDA3937_RS14320 and DDA3937_RS14325 are separated by 2 nucleotides DDA3937_RS14325 and DDA3937_RS14330 are separated by 117 nucleotides DDA3937_RS14330 and DDA3937_RS14335 are separated by 19 nucleotides
DDA3937_RS14315: DDA3937_RS14315 - NADH-quinone oxidoreductase subunit NuoF, at 3,282,113 to 3,283,462
_RS14315
DDA3937_RS14320: DDA3937_RS14320 - NADH-quinone oxidoreductase subunit NuoE, at 3,283,459 to 3,283,989
_RS14320
DDA3937_RS14325: DDA3937_RS14325 - NADH-quinone oxidoreductase subunit C/D, at 3,283,992 to 3,285,791
_RS14325
DDA3937_RS14330: DDA3937_RS14330 - NADH-quinone oxidoreductase subunit B, at 3,285,909 to 3,286,583
_RS14330
DDA3937_RS14335: DDA3937_RS14335 - NADH-quinone oxidoreductase subunit A, at 3,286,603 to 3,287,046
_RS14335
Position (kb)
3283
3284
3285
3286 Strain fitness (log2 ratio)
-1
0
1 at 3283.617 kb on + strand, within DDA3937_RS14320 at 3285.590 kb on + strand, within DDA3937_RS14325 at 3285.810 kb on - strand at 3285.819 kb on - strand at 3285.890 kb on - strand at 3286.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 3,283,617 + DDA3937_RS14320 0.30 -0.5 3,285,590 + DDA3937_RS14325 0.89 -1.0 3,285,810 - -0.1 3,285,819 - -0.5 3,285,890 - -0.4 3,286,605 - -1.4
Or see this region's nucleotide sequence