Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS02660 and DDA3937_RS02665 are separated by 164 nucleotides DDA3937_RS02665 and DDA3937_RS02670 are separated by 2 nucleotides DDA3937_RS02670 and DDA3937_RS02675 are separated by 58 nucleotides DDA3937_RS02675 and DDA3937_RS02680 are separated by 900 nucleotides
DDA3937_RS02660: DDA3937_RS02660 - RNA-directed DNA polymerase, at 590,563 to 591,906
_RS02660
DDA3937_RS02665: DDA3937_RS02665 - type II toxin-antitoxin system toxin CcdB, at 592,071 to 592,376
_RS02665
DDA3937_RS02670: DDA3937_RS02670 - type II toxin-antitoxin system antitoxin CcdA, at 592,379 to 592,597
_RS02670
DDA3937_RS02675: DDA3937_RS02675 - hypothetical protein, at 592,656 to 593,399
_RS02675
DDA3937_RS02680: DDA3937_RS02680 - hypothetical protein, at 594,300 to 594,650
_RS02680
Position (kb) 592
593
594 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 591.751 kb on - strand, within DDA3937_RS02660 at 591.929 kb on + strand at 591.935 kb on - strand at 591.962 kb on - strand at 591.962 kb on - strand at 592.005 kb on + strand at 592.005 kb on + strand at 592.040 kb on + strand at 592.041 kb on - strand at 592.140 kb on + strand, within DDA3937_RS02665 at 592.236 kb on - strand, within DDA3937_RS02665 at 592.557 kb on + strand, within DDA3937_RS02670 at 592.561 kb on + strand, within DDA3937_RS02670 at 592.562 kb on - strand at 592.620 kb on + strand at 592.629 kb on + strand at 592.632 kb on - strand at 592.633 kb on + strand at 592.634 kb on - strand at 592.658 kb on - strand at 592.697 kb on + strand at 592.697 kb on + strand at 592.697 kb on + strand at 592.698 kb on - strand at 592.698 kb on - strand at 592.698 kb on - strand at 592.699 kb on + strand at 592.700 kb on - strand at 592.763 kb on - strand, within DDA3937_RS02675 at 592.763 kb on - strand, within DDA3937_RS02675 at 592.763 kb on - strand, within DDA3937_RS02675 at 592.822 kb on + strand, within DDA3937_RS02675 at 592.822 kb on + strand, within DDA3937_RS02675 at 592.822 kb on + strand, within DDA3937_RS02675 at 592.822 kb on + strand, within DDA3937_RS02675 at 592.823 kb on - strand, within DDA3937_RS02675 at 592.823 kb on - strand, within DDA3937_RS02675 at 592.831 kb on + strand, within DDA3937_RS02675 at 592.831 kb on + strand, within DDA3937_RS02675 at 592.832 kb on - strand, within DDA3937_RS02675 at 592.832 kb on - strand, within DDA3937_RS02675 at 592.875 kb on - strand, within DDA3937_RS02675 at 593.064 kb on - strand, within DDA3937_RS02675 at 593.155 kb on - strand, within DDA3937_RS02675 at 593.250 kb on + strand, within DDA3937_RS02675 at 593.257 kb on + strand, within DDA3937_RS02675 at 593.258 kb on - strand, within DDA3937_RS02675 at 593.276 kb on + strand, within DDA3937_RS02675 at 593.276 kb on + strand, within DDA3937_RS02675 at 593.315 kb on - strand, within DDA3937_RS02675 at 593.315 kb on - strand, within DDA3937_RS02675 at 593.315 kb on - strand, within DDA3937_RS02675 at 593.438 kb on + strand at 593.438 kb on + strand at 593.439 kb on - strand at 593.554 kb on - strand at 593.786 kb on + strand at 593.822 kb on + strand at 593.822 kb on + strand at 593.823 kb on - strand at 593.859 kb on - strand at 594.215 kb on - strand at 594.238 kb on + strand at 594.366 kb on + strand, within DDA3937_RS02680 at 594.366 kb on + strand, within DDA3937_RS02680 at 594.366 kb on + strand, within DDA3937_RS02680 at 594.367 kb on - strand, within DDA3937_RS02680
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 591,751 - DDA3937_RS02660 0.88 -0.7 591,929 + -0.4 591,935 - +0.1 591,962 - -0.4 591,962 - -0.1 592,005 + -0.5 592,005 + +0.2 592,040 + -0.7 592,041 - -1.6 592,140 + DDA3937_RS02665 0.23 -1.3 592,236 - DDA3937_RS02665 0.54 -0.6 592,557 + DDA3937_RS02670 0.81 +1.1 592,561 + DDA3937_RS02670 0.83 +2.2 592,562 - +0.6 592,620 + +0.5 592,629 + +0.2 592,632 - +0.0 592,633 + +0.6 592,634 - -0.8 592,658 - +2.8 592,697 + -0.1 592,697 + -0.2 592,697 + -1.0 592,698 - -0.9 592,698 - -0.0 592,698 - +0.2 592,699 + +0.2 592,700 - +1.5 592,763 - DDA3937_RS02675 0.14 -0.4 592,763 - DDA3937_RS02675 0.14 -0.6 592,763 - DDA3937_RS02675 0.14 -0.4 592,822 + DDA3937_RS02675 0.22 -0.6 592,822 + DDA3937_RS02675 0.22 -1.0 592,822 + DDA3937_RS02675 0.22 -3.7 592,822 + DDA3937_RS02675 0.22 -1.3 592,823 - DDA3937_RS02675 0.22 -0.0 592,823 - DDA3937_RS02675 0.22 +0.3 592,831 + DDA3937_RS02675 0.24 -0.1 592,831 + DDA3937_RS02675 0.24 -0.2 592,832 - DDA3937_RS02675 0.24 -0.8 592,832 - DDA3937_RS02675 0.24 -0.8 592,875 - DDA3937_RS02675 0.29 -0.7 593,064 - DDA3937_RS02675 0.55 +0.2 593,155 - DDA3937_RS02675 0.67 -0.6 593,250 + DDA3937_RS02675 0.80 -1.8 593,257 + DDA3937_RS02675 0.81 -0.7 593,258 - DDA3937_RS02675 0.81 +0.6 593,276 + DDA3937_RS02675 0.83 -1.1 593,276 + DDA3937_RS02675 0.83 -0.6 593,315 - DDA3937_RS02675 0.89 -0.2 593,315 - DDA3937_RS02675 0.89 -0.8 593,315 - DDA3937_RS02675 0.89 -1.9 593,438 + -0.9 593,438 + -0.0 593,439 - +0.3 593,554 - -1.0 593,786 + -0.9 593,822 + -0.5 593,822 + -0.8 593,823 - -2.1 593,859 - -1.0 594,215 - +0.5 594,238 + -0.8 594,366 + DDA3937_RS02680 0.19 -0.8 594,366 + DDA3937_RS02680 0.19 -0.6 594,366 + DDA3937_RS02680 0.19 +1.1 594,367 - DDA3937_RS02680 0.19 +1.4
Or see this region's nucleotide sequence