Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01170 and DZA65_RS01175 are separated by 275 nucleotides DZA65_RS01175 and DZA65_RS01180 are separated by 259 nucleotides DZA65_RS01180 and DZA65_RS22770 are separated by 24 nucleotides DZA65_RS22770 and DZA65_RS01185 overlap by 4 nucleotides
DZA65_RS01170: DZA65_RS01170 - DUF1176 domain-containing protein, at 263,862 to 264,959
_RS01170
DZA65_RS01175: DZA65_RS01175 - DUF1127 domain-containing protein, at 265,235 to 265,414
_RS01175
DZA65_RS01180: DZA65_RS01180 - DUF1127 domain-containing protein, at 265,674 to 265,865
_RS01180
DZA65_RS22770: DZA65_RS22770 - hypothetical protein, at 265,890 to 266,033
_RS22770
DZA65_RS01185: DZA65_RS01185 - PLP-dependent aminotransferase family protein, at 266,030 to 267,460
_RS01185
Position (kb) 265
266
267 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 264.896 kb on + strand at 264.896 kb on + strand at 264.896 kb on + strand at 264.897 kb on - strand at 264.897 kb on - strand at 265.039 kb on - strand at 265.039 kb on - strand at 265.057 kb on + strand at 265.074 kb on + strand at 265.074 kb on + strand at 265.075 kb on - strand at 265.075 kb on - strand at 265.075 kb on - strand at 265.075 kb on - strand at 265.143 kb on - strand at 265.143 kb on - strand at 265.143 kb on - strand at 265.151 kb on + strand at 265.151 kb on + strand at 265.152 kb on - strand at 265.152 kb on - strand at 265.154 kb on + strand at 265.208 kb on + strand at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.298 kb on - strand, within DZA65_RS01175 at 265.298 kb on - strand, within DZA65_RS01175 at 265.301 kb on + strand, within DZA65_RS01175 at 265.301 kb on + strand, within DZA65_RS01175 at 265.301 kb on + strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.344 kb on + strand, within DZA65_RS01175 at 265.344 kb on + strand, within DZA65_RS01175 at 265.401 kb on + strand at 265.401 kb on + strand at 265.421 kb on - strand at 265.439 kb on + strand at 265.440 kb on - strand at 265.440 kb on - strand at 265.442 kb on + strand at 265.442 kb on + strand at 265.442 kb on + strand at 265.442 kb on + strand at 265.443 kb on - strand at 265.443 kb on - strand at 265.443 kb on - strand at 265.443 kb on - strand at 265.472 kb on + strand at 265.472 kb on + strand at 265.502 kb on + strand at 265.503 kb on - strand at 265.518 kb on + strand at 265.519 kb on - strand at 265.632 kb on - strand at 265.650 kb on + strand at 265.650 kb on + strand at 265.651 kb on - strand at 265.651 kb on - strand at 265.718 kb on + strand, within DZA65_RS01180 at 265.745 kb on + strand, within DZA65_RS01180 at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.857 kb on + strand at 265.857 kb on + strand at 265.857 kb on + strand at 265.857 kb on + strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.916 kb on + strand, within DZA65_RS22770 at 265.963 kb on + strand, within DZA65_RS22770 at 265.963 kb on + strand, within DZA65_RS22770 at 265.963 kb on + strand, within DZA65_RS22770 at 265.964 kb on - strand, within DZA65_RS22770 at 265.964 kb on - strand, within DZA65_RS22770 at 265.964 kb on - strand, within DZA65_RS22770 at 266.001 kb on + strand, within DZA65_RS22770 at 266.023 kb on + strand at 266.023 kb on + strand at 266.023 kb on + strand at 266.024 kb on - strand at 266.024 kb on - strand at 266.029 kb on + strand at 266.029 kb on + strand at 266.030 kb on - strand at 266.030 kb on - strand at 266.030 kb on - strand at 266.058 kb on + strand at 266.087 kb on + strand at 266.087 kb on + strand at 266.087 kb on + strand at 266.088 kb on - strand at 266.115 kb on + strand at 266.116 kb on - strand at 266.124 kb on + strand at 266.124 kb on + strand at 266.124 kb on + strand at 266.146 kb on + strand at 266.146 kb on + strand at 266.168 kb on + strand at 266.169 kb on - strand at 266.169 kb on - strand at 266.169 kb on - strand at 266.169 kb on - strand at 266.169 kb on - strand at 266.227 kb on + strand, within DZA65_RS01185 at 266.400 kb on + strand, within DZA65_RS01185 at 266.410 kb on + strand, within DZA65_RS01185 at 266.411 kb on - strand, within DZA65_RS01185 at 266.472 kb on + strand, within DZA65_RS01185 at 266.472 kb on + strand, within DZA65_RS01185 at 266.560 kb on + strand, within DZA65_RS01185 at 266.560 kb on + strand, within DZA65_RS01185 at 266.596 kb on + strand, within DZA65_RS01185 at 266.596 kb on + strand, within DZA65_RS01185 at 266.596 kb on + strand, within DZA65_RS01185 at 266.597 kb on - strand, within DZA65_RS01185 at 266.597 kb on - strand, within DZA65_RS01185 at 266.597 kb on - strand, within DZA65_RS01185 at 266.653 kb on - strand, within DZA65_RS01185 at 266.653 kb on - strand, within DZA65_RS01185 at 266.653 kb on - strand, within DZA65_RS01185 at 266.876 kb on + strand, within DZA65_RS01185 at 266.876 kb on + strand, within DZA65_RS01185 at 266.876 kb on + strand, within DZA65_RS01185 at 266.877 kb on - strand, within DZA65_RS01185 at 266.877 kb on - strand, within DZA65_RS01185 at 266.877 kb on - strand, within DZA65_RS01185 at 266.877 kb on - strand, within DZA65_RS01185 at 266.891 kb on + strand at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.891 kb on + strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.892 kb on - strand, within DZA65_RS01185 at 266.897 kb on + strand, within DZA65_RS01185 at 266.897 kb on + strand, within DZA65_RS01185 at 266.898 kb on - strand, within DZA65_RS01185 at 266.898 kb on - strand, within DZA65_RS01185 at 266.898 kb on - strand, within DZA65_RS01185 at 266.911 kb on + strand, within DZA65_RS01185 at 266.912 kb on - strand, within DZA65_RS01185 at 266.912 kb on - strand, within DZA65_RS01185
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 264,896 + +0.1 264,896 + -0.8 264,896 + -1.7 264,897 - -1.5 264,897 - +0.3 265,039 - -2.5 265,039 - -1.3 265,057 + +0.1 265,074 + +1.1 265,074 + -0.3 265,075 - +1.0 265,075 - +1.9 265,075 - +1.1 265,075 - -0.5 265,143 - -0.5 265,143 - -1.5 265,143 - -0.5 265,151 + -0.7 265,151 + -0.8 265,152 - -1.0 265,152 - -1.7 265,154 + -1.7 265,208 + -0.3 265,297 + DZA65_RS01175 0.34 +1.3 265,297 + DZA65_RS01175 0.34 -1.5 265,297 + DZA65_RS01175 0.34 +1.9 265,297 + DZA65_RS01175 0.34 +0.1 265,297 + DZA65_RS01175 0.34 -0.0 265,298 - DZA65_RS01175 0.35 -0.4 265,298 - DZA65_RS01175 0.35 -1.8 265,301 + DZA65_RS01175 0.37 -0.6 265,301 + DZA65_RS01175 0.37 -0.6 265,301 + DZA65_RS01175 0.37 +0.1 265,302 - DZA65_RS01175 0.37 +0.0 265,302 - DZA65_RS01175 0.37 -0.7 265,302 - DZA65_RS01175 0.37 +0.8 265,302 - DZA65_RS01175 0.37 +0.3 265,344 + DZA65_RS01175 0.61 -0.2 265,344 + DZA65_RS01175 0.61 +1.3 265,401 + -1.0 265,401 + -0.4 265,421 - +0.7 265,439 + +0.6 265,440 - -0.2 265,440 - -1.3 265,442 + -0.4 265,442 + -0.4 265,442 + -2.0 265,442 + -0.1 265,443 - +0.6 265,443 - -0.7 265,443 - -1.5 265,443 - +0.8 265,472 + -0.3 265,472 + +0.3 265,502 + -0.3 265,503 - -0.3 265,518 + -0.6 265,519 - -0.7 265,632 - -1.6 265,650 + +1.1 265,650 + +0.0 265,651 - -0.3 265,651 - +0.2 265,718 + DZA65_RS01180 0.23 +0.2 265,745 + DZA65_RS01180 0.37 +0.1 265,854 + +0.3 265,854 + +1.3 265,854 + -0.4 265,854 + -0.6 265,854 + -0.1 265,854 + +0.7 265,854 + +0.3 265,854 + +0.2 265,854 + -0.4 265,854 + -1.5 265,854 + +0.9 265,854 + +0.1 265,854 + +0.0 265,855 - -0.8 265,855 - +0.1 265,855 - -0.1 265,855 - +0.3 265,855 - -0.4 265,857 + -0.3 265,857 + +0.3 265,857 + +1.0 265,857 + -1.5 265,858 - -0.9 265,858 - -0.3 265,858 - +0.1 265,858 - -0.7 265,858 - -3.2 265,858 - +0.2 265,858 - -0.3 265,858 - +0.6 265,893 + -0.1 265,893 + -0.3 265,893 + -0.4 265,893 + +1.9 265,893 + -0.4 265,893 + +0.9 265,893 + +0.4 265,894 - +0.4 265,894 - +0.2 265,894 - -1.6 265,894 - -0.7 265,894 - +0.7 265,894 - -0.8 265,894 - -1.7 265,894 - -0.2 265,894 - -0.1 265,894 - +0.2 265,894 - +0.6 265,916 + DZA65_RS22770 0.18 -2.7 265,963 + DZA65_RS22770 0.51 -0.3 265,963 + DZA65_RS22770 0.51 -1.9 265,963 + DZA65_RS22770 0.51 +0.7 265,964 - DZA65_RS22770 0.51 -1.1 265,964 - DZA65_RS22770 0.51 -0.7 265,964 - DZA65_RS22770 0.51 -0.3 266,001 + DZA65_RS22770 0.77 -0.7 266,023 + +0.1 266,023 + +1.5 266,023 + -0.6 266,024 - -0.1 266,024 - -0.3 266,029 + -0.9 266,029 + -0.7 266,030 - +0.7 266,030 - +0.6 266,030 - -1.8 266,058 + -1.0 266,087 + +1.0 266,087 + +0.4 266,087 + +0.4 266,088 - -0.7 266,115 + +1.7 266,116 - +0.3 266,124 + -0.2 266,124 + -0.3 266,124 + +1.5 266,146 + +0.7 266,146 + -1.5 266,168 + +0.5 266,169 - -1.4 266,169 - -0.3 266,169 - -2.3 266,169 - -0.3 266,169 - -0.7 266,227 + DZA65_RS01185 0.14 -0.1 266,400 + DZA65_RS01185 0.26 +0.3 266,410 + DZA65_RS01185 0.27 +0.5 266,411 - DZA65_RS01185 0.27 -0.1 266,472 + DZA65_RS01185 0.31 -0.2 266,472 + DZA65_RS01185 0.31 -1.0 266,560 + DZA65_RS01185 0.37 +1.3 266,560 + DZA65_RS01185 0.37 +1.1 266,596 + DZA65_RS01185 0.40 -0.0 266,596 + DZA65_RS01185 0.40 +0.0 266,596 + DZA65_RS01185 0.40 +0.7 266,597 - DZA65_RS01185 0.40 -0.8 266,597 - DZA65_RS01185 0.40 -1.3 266,597 - DZA65_RS01185 0.40 +2.1 266,653 - DZA65_RS01185 0.44 +0.2 266,653 - DZA65_RS01185 0.44 -1.0 266,653 - DZA65_RS01185 0.44 +0.2 266,876 + DZA65_RS01185 0.59 +0.4 266,876 + DZA65_RS01185 0.59 +2.7 266,876 + DZA65_RS01185 0.59 +0.5 266,877 - DZA65_RS01185 0.59 +0.9 266,877 - DZA65_RS01185 0.59 -0.3 266,877 - DZA65_RS01185 0.59 +0.3 266,877 - DZA65_RS01185 0.59 +1.6 266,891 + +1.3 266,891 + DZA65_RS01185 0.60 -0.4 266,891 + DZA65_RS01185 0.60 -0.4 266,891 + DZA65_RS01185 0.60 +0.2 266,891 + DZA65_RS01185 0.60 -0.4 266,891 + DZA65_RS01185 0.60 +0.1 266,891 + DZA65_RS01185 0.60 -0.3 266,891 + DZA65_RS01185 0.60 -0.1 266,891 + DZA65_RS01185 0.60 -0.9 266,892 - DZA65_RS01185 0.60 +0.8 266,892 - DZA65_RS01185 0.60 +0.6 266,892 - DZA65_RS01185 0.60 -2.2 266,892 - DZA65_RS01185 0.60 -0.8 266,892 - DZA65_RS01185 0.60 +0.5 266,892 - DZA65_RS01185 0.60 -0.0 266,892 - DZA65_RS01185 0.60 -0.4 266,892 - DZA65_RS01185 0.60 -1.3 266,892 - DZA65_RS01185 0.60 +0.3 266,897 + DZA65_RS01185 0.61 -0.4 266,897 + DZA65_RS01185 0.61 +0.0 266,898 - DZA65_RS01185 0.61 +0.7 266,898 - DZA65_RS01185 0.61 +0.1 266,898 - DZA65_RS01185 0.61 +0.3 266,911 + DZA65_RS01185 0.62 +0.2 266,912 - DZA65_RS01185 0.62 -0.7 266,912 - DZA65_RS01185 0.62 -0.3
Or see this region's nucleotide sequence