Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS17025 and DZA65_RS17030 are separated by 43 nucleotides DZA65_RS17030 and DZA65_RS17035 are separated by 226 nucleotides DZA65_RS17035 and DZA65_RS17040 are separated by 15 nucleotides
DZA65_RS17025: DZA65_RS17025 - alpha/beta hydrolase, at 3,673,406 to 3,674,368
_RS17025
DZA65_RS17030: DZA65_RS17030 - hypothetical protein, at 3,674,412 to 3,675,689
_RS17030
DZA65_RS17035: DZA65_RS17035 - helix-turn-helix transcriptional regulator, at 3,675,916 to 3,676,245
_RS17035
DZA65_RS17040: DZA65_RS17040 - IS630 family transposase, at 3,676,261 to 3,677,298
_RS17040
Position (kb)
3674
3675
3676 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3673.445 kb on + strand at 3673.457 kb on - strand at 3673.510 kb on - strand, within DZA65_RS17025 at 3673.510 kb on - strand, within DZA65_RS17025 at 3673.558 kb on + strand, within DZA65_RS17025 at 3673.558 kb on + strand, within DZA65_RS17025 at 3673.575 kb on - strand, within DZA65_RS17025 at 3673.575 kb on - strand, within DZA65_RS17025 at 3673.631 kb on - strand, within DZA65_RS17025 at 3673.632 kb on + strand, within DZA65_RS17025 at 3673.632 kb on + strand, within DZA65_RS17025 at 3673.673 kb on + strand, within DZA65_RS17025 at 3673.709 kb on + strand, within DZA65_RS17025 at 3673.710 kb on - strand, within DZA65_RS17025 at 3673.710 kb on - strand, within DZA65_RS17025 at 3673.710 kb on - strand, within DZA65_RS17025 at 3673.710 kb on - strand, within DZA65_RS17025 at 3673.710 kb on - strand, within DZA65_RS17025 at 3673.787 kb on + strand, within DZA65_RS17025 at 3673.804 kb on - strand, within DZA65_RS17025 at 3673.805 kb on + strand, within DZA65_RS17025 at 3673.805 kb on + strand, within DZA65_RS17025 at 3673.806 kb on - strand, within DZA65_RS17025 at 3673.806 kb on - strand, within DZA65_RS17025 at 3673.856 kb on - strand, within DZA65_RS17025 at 3673.856 kb on - strand, within DZA65_RS17025 at 3673.947 kb on - strand, within DZA65_RS17025 at 3674.062 kb on - strand, within DZA65_RS17025 at 3674.108 kb on - strand, within DZA65_RS17025 at 3674.138 kb on - strand, within DZA65_RS17025 at 3674.181 kb on - strand, within DZA65_RS17025 at 3674.188 kb on + strand, within DZA65_RS17025 at 3674.218 kb on + strand, within DZA65_RS17025 at 3674.221 kb on - strand, within DZA65_RS17025 at 3674.375 kb on - strand at 3674.427 kb on + strand at 3674.494 kb on + strand at 3674.495 kb on - strand at 3674.495 kb on - strand at 3674.495 kb on - strand at 3674.517 kb on - strand at 3674.527 kb on + strand at 3674.527 kb on + strand at 3674.527 kb on + strand at 3674.528 kb on - strand at 3674.528 kb on - strand at 3674.535 kb on + strand at 3674.536 kb on - strand at 3674.536 kb on - strand at 3674.570 kb on + strand, within DZA65_RS17030 at 3674.570 kb on + strand, within DZA65_RS17030 at 3674.571 kb on - strand, within DZA65_RS17030 at 3674.572 kb on + strand, within DZA65_RS17030 at 3674.572 kb on + strand, within DZA65_RS17030 at 3674.607 kb on - strand, within DZA65_RS17030 at 3674.607 kb on - strand, within DZA65_RS17030 at 3674.609 kb on + strand, within DZA65_RS17030 at 3674.609 kb on + strand, within DZA65_RS17030 at 3674.609 kb on + strand, within DZA65_RS17030 at 3674.610 kb on - strand, within DZA65_RS17030 at 3674.636 kb on - strand, within DZA65_RS17030 at 3674.636 kb on - strand, within DZA65_RS17030 at 3674.714 kb on - strand, within DZA65_RS17030 at 3674.834 kb on + strand, within DZA65_RS17030 at 3674.834 kb on + strand, within DZA65_RS17030 at 3674.881 kb on - strand, within DZA65_RS17030 at 3674.883 kb on + strand, within DZA65_RS17030 at 3674.955 kb on - strand, within DZA65_RS17030 at 3674.987 kb on + strand, within DZA65_RS17030 at 3674.987 kb on + strand, within DZA65_RS17030 at 3674.987 kb on + strand, within DZA65_RS17030 at 3674.987 kb on + strand, within DZA65_RS17030 at 3674.988 kb on - strand, within DZA65_RS17030 at 3674.988 kb on - strand, within DZA65_RS17030 at 3674.989 kb on + strand, within DZA65_RS17030 at 3674.990 kb on - strand, within DZA65_RS17030 at 3674.990 kb on - strand, within DZA65_RS17030 at 3674.990 kb on - strand, within DZA65_RS17030 at 3674.993 kb on + strand, within DZA65_RS17030 at 3675.056 kb on - strand, within DZA65_RS17030 at 3675.056 kb on - strand, within DZA65_RS17030 at 3675.062 kb on + strand, within DZA65_RS17030 at 3675.093 kb on + strand, within DZA65_RS17030 at 3675.093 kb on + strand, within DZA65_RS17030 at 3675.093 kb on + strand, within DZA65_RS17030 at 3675.093 kb on + strand, within DZA65_RS17030 at 3675.191 kb on + strand, within DZA65_RS17030 at 3675.191 kb on + strand, within DZA65_RS17030 at 3675.192 kb on - strand, within DZA65_RS17030 at 3675.194 kb on + strand, within DZA65_RS17030 at 3675.194 kb on + strand, within DZA65_RS17030 at 3675.233 kb on + strand, within DZA65_RS17030 at 3675.266 kb on - strand, within DZA65_RS17030 at 3675.377 kb on - strand, within DZA65_RS17030 at 3675.380 kb on + strand, within DZA65_RS17030 at 3675.380 kb on + strand, within DZA65_RS17030 at 3675.381 kb on - strand, within DZA65_RS17030 at 3675.381 kb on - strand, within DZA65_RS17030 at 3675.444 kb on - strand, within DZA65_RS17030 at 3675.449 kb on - strand, within DZA65_RS17030 at 3675.461 kb on - strand, within DZA65_RS17030 at 3675.464 kb on + strand, within DZA65_RS17030 at 3675.521 kb on + strand, within DZA65_RS17030 at 3675.534 kb on - strand, within DZA65_RS17030 at 3675.592 kb on - strand at 3675.797 kb on - strand at 3675.799 kb on + strand at 3676.126 kb on - strand, within DZA65_RS17035 at 3676.126 kb on - strand, within DZA65_RS17035 at 3676.129 kb on + strand, within DZA65_RS17035 at 3676.207 kb on + strand, within DZA65_RS17035 at 3676.211 kb on - strand, within DZA65_RS17035 at 3676.211 kb on - strand, within DZA65_RS17035 at 3676.250 kb on - strand at 3676.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,673,445 + -1.9 3,673,457 - +0.9 3,673,510 - DZA65_RS17025 0.11 -0.1 3,673,510 - DZA65_RS17025 0.11 -2.0 3,673,558 + DZA65_RS17025 0.16 -0.1 3,673,558 + DZA65_RS17025 0.16 -1.1 3,673,575 - DZA65_RS17025 0.18 -1.1 3,673,575 - DZA65_RS17025 0.18 +0.9 3,673,631 - DZA65_RS17025 0.23 +0.4 3,673,632 + DZA65_RS17025 0.23 -1.2 3,673,632 + DZA65_RS17025 0.23 +0.4 3,673,673 + DZA65_RS17025 0.28 -1.3 3,673,709 + DZA65_RS17025 0.31 -1.1 3,673,710 - DZA65_RS17025 0.32 -2.1 3,673,710 - DZA65_RS17025 0.32 -0.3 3,673,710 - DZA65_RS17025 0.32 -0.8 3,673,710 - DZA65_RS17025 0.32 -0.8 3,673,710 - DZA65_RS17025 0.32 +0.0 3,673,787 + DZA65_RS17025 0.40 -0.7 3,673,804 - DZA65_RS17025 0.41 -0.8 3,673,805 + DZA65_RS17025 0.41 +0.6 3,673,805 + DZA65_RS17025 0.41 +1.0 3,673,806 - DZA65_RS17025 0.42 +0.0 3,673,806 - DZA65_RS17025 0.42 -0.5 3,673,856 - DZA65_RS17025 0.47 +0.5 3,673,856 - DZA65_RS17025 0.47 +1.0 3,673,947 - DZA65_RS17025 0.56 -0.4 3,674,062 - DZA65_RS17025 0.68 +0.2 3,674,108 - DZA65_RS17025 0.73 -1.0 3,674,138 - DZA65_RS17025 0.76 -1.1 3,674,181 - DZA65_RS17025 0.80 -1.6 3,674,188 + DZA65_RS17025 0.81 +0.4 3,674,218 + DZA65_RS17025 0.84 +0.6 3,674,221 - DZA65_RS17025 0.85 -1.0 3,674,375 - -0.6 3,674,427 + -0.7 3,674,494 + +1.3 3,674,495 - -0.2 3,674,495 - -0.6 3,674,495 - +0.2 3,674,517 - +0.0 3,674,527 + +0.4 3,674,527 + +0.6 3,674,527 + +0.8 3,674,528 - -0.6 3,674,528 - -2.6 3,674,535 + +0.2 3,674,536 - -1.3 3,674,536 - +0.7 3,674,570 + DZA65_RS17030 0.12 -1.1 3,674,570 + DZA65_RS17030 0.12 +1.4 3,674,571 - DZA65_RS17030 0.12 -0.4 3,674,572 + DZA65_RS17030 0.13 -0.0 3,674,572 + DZA65_RS17030 0.13 -2.0 3,674,607 - DZA65_RS17030 0.15 -0.9 3,674,607 - DZA65_RS17030 0.15 -0.2 3,674,609 + DZA65_RS17030 0.15 +1.3 3,674,609 + DZA65_RS17030 0.15 +0.4 3,674,609 + DZA65_RS17030 0.15 -1.2 3,674,610 - DZA65_RS17030 0.15 -0.8 3,674,636 - DZA65_RS17030 0.18 -0.6 3,674,636 - DZA65_RS17030 0.18 +0.2 3,674,714 - DZA65_RS17030 0.24 -2.4 3,674,834 + DZA65_RS17030 0.33 -0.6 3,674,834 + DZA65_RS17030 0.33 -0.3 3,674,881 - DZA65_RS17030 0.37 -1.8 3,674,883 + DZA65_RS17030 0.37 -0.8 3,674,955 - DZA65_RS17030 0.42 -3.0 3,674,987 + DZA65_RS17030 0.45 -0.7 3,674,987 + DZA65_RS17030 0.45 -0.1 3,674,987 + DZA65_RS17030 0.45 +0.7 3,674,987 + DZA65_RS17030 0.45 -2.1 3,674,988 - DZA65_RS17030 0.45 -1.6 3,674,988 - DZA65_RS17030 0.45 -1.1 3,674,989 + DZA65_RS17030 0.45 -0.3 3,674,990 - DZA65_RS17030 0.45 -1.0 3,674,990 - DZA65_RS17030 0.45 -0.0 3,674,990 - DZA65_RS17030 0.45 -0.6 3,674,993 + DZA65_RS17030 0.45 -0.7 3,675,056 - DZA65_RS17030 0.50 -1.1 3,675,056 - DZA65_RS17030 0.50 +0.1 3,675,062 + DZA65_RS17030 0.51 -0.4 3,675,093 + DZA65_RS17030 0.53 -0.6 3,675,093 + DZA65_RS17030 0.53 +0.4 3,675,093 + DZA65_RS17030 0.53 +0.4 3,675,093 + DZA65_RS17030 0.53 +1.0 3,675,191 + DZA65_RS17030 0.61 -0.4 3,675,191 + DZA65_RS17030 0.61 +0.2 3,675,192 - DZA65_RS17030 0.61 +0.2 3,675,194 + DZA65_RS17030 0.61 +0.2 3,675,194 + DZA65_RS17030 0.61 +0.7 3,675,233 + DZA65_RS17030 0.64 +0.4 3,675,266 - DZA65_RS17030 0.67 -0.0 3,675,377 - DZA65_RS17030 0.76 -2.1 3,675,380 + DZA65_RS17030 0.76 +1.4 3,675,380 + DZA65_RS17030 0.76 +0.6 3,675,381 - DZA65_RS17030 0.76 -0.3 3,675,381 - DZA65_RS17030 0.76 -1.2 3,675,444 - DZA65_RS17030 0.81 -1.9 3,675,449 - DZA65_RS17030 0.81 -1.4 3,675,461 - DZA65_RS17030 0.82 -0.8 3,675,464 + DZA65_RS17030 0.82 +1.3 3,675,521 + DZA65_RS17030 0.87 -2.2 3,675,534 - DZA65_RS17030 0.88 +0.0 3,675,592 - +0.3 3,675,797 - +0.0 3,675,799 + -0.2 3,676,126 - DZA65_RS17035 0.64 -1.8 3,676,126 - DZA65_RS17035 0.64 +0.2 3,676,129 + DZA65_RS17035 0.65 -1.6 3,676,207 + DZA65_RS17035 0.88 -0.6 3,676,211 - DZA65_RS17035 0.89 +0.1 3,676,211 - DZA65_RS17035 0.89 -1.0 3,676,250 - +1.3 3,676,250 - -1.8
Or see this region's nucleotide sequence