Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS13305 and DZA65_RS13310 are separated by 14 nucleotides DZA65_RS13310 and DZA65_RS13315 are separated by 547 nucleotides DZA65_RS13315 and DZA65_RS13320 are separated by 71 nucleotides DZA65_RS13320 and DZA65_RS13325 overlap by 1 nucleotides
DZA65_RS13305: DZA65_RS13305 - Fe-S cluster assembly protein SufB, at 2,847,663 to 2,849,162
_RS13305
DZA65_RS13310: DZA65_RS13310 - Fe-S cluster assembly scaffold SufA, at 2,849,177 to 2,849,548
_RS13310
DZA65_RS13315: DZA65_RS13315 - hypothetical protein, at 2,850,096 to 2,850,497
_RS13315
DZA65_RS13320: DZA65_RS13320 - hotdog fold thioesterase, at 2,850,569 to 2,850,991
_RS13320
DZA65_RS13325: DZA65_RS13325 - FAD-binding oxidoreductase, at 2,850,991 to 2,854,050
_RS13325
Position (kb)
2850
2851 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2849.099 kb on + strand at 2849.099 kb on + strand at 2849.099 kb on + strand at 2849.099 kb on + strand at 2849.099 kb on + strand at 2849.099 kb on + strand at 2849.100 kb on - strand at 2849.100 kb on - strand at 2849.101 kb on + strand at 2849.102 kb on - strand at 2849.145 kb on + strand at 2849.146 kb on - strand at 2849.146 kb on - strand at 2849.146 kb on - strand at 2849.146 kb on - strand at 2849.146 kb on - strand at 2849.240 kb on + strand, within DZA65_RS13310 at 2849.240 kb on + strand, within DZA65_RS13310 at 2849.338 kb on - strand, within DZA65_RS13310 at 2849.378 kb on + strand, within DZA65_RS13310 at 2849.378 kb on + strand, within DZA65_RS13310 at 2849.379 kb on - strand, within DZA65_RS13310 at 2849.379 kb on - strand, within DZA65_RS13310 at 2849.383 kb on - strand, within DZA65_RS13310 at 2849.464 kb on + strand, within DZA65_RS13310 at 2849.465 kb on - strand, within DZA65_RS13310 at 2849.531 kb on + strand at 2849.531 kb on + strand at 2849.531 kb on + strand at 2849.532 kb on - strand at 2849.532 kb on - strand at 2849.532 kb on - strand at 2849.532 kb on - strand at 2849.532 kb on - strand at 2849.570 kb on + strand at 2849.570 kb on + strand at 2849.649 kb on - strand at 2849.678 kb on - strand at 2849.681 kb on + strand at 2849.724 kb on + strand at 2849.724 kb on + strand at 2849.724 kb on + strand at 2849.725 kb on - strand at 2849.725 kb on - strand at 2849.725 kb on - strand at 2849.806 kb on + strand at 2849.820 kb on + strand at 2849.821 kb on - strand at 2849.821 kb on - strand at 2849.823 kb on - strand at 2849.823 kb on - strand at 2849.841 kb on - strand at 2849.842 kb on + strand at 2849.843 kb on - strand at 2849.843 kb on - strand at 2849.843 kb on - strand at 2849.846 kb on + strand at 2849.847 kb on - strand at 2849.854 kb on + strand at 2849.865 kb on - strand at 2849.881 kb on + strand at 2849.890 kb on + strand at 2849.890 kb on + strand at 2849.892 kb on + strand at 2849.964 kb on - strand at 2849.964 kb on - strand at 2850.019 kb on + strand at 2850.083 kb on + strand at 2850.084 kb on - strand at 2850.084 kb on - strand at 2850.084 kb on - strand at 2850.095 kb on + strand at 2850.096 kb on - strand at 2850.096 kb on - strand at 2850.174 kb on + strand, within DZA65_RS13315 at 2850.231 kb on + strand, within DZA65_RS13315 at 2850.231 kb on + strand, within DZA65_RS13315 at 2850.232 kb on - strand, within DZA65_RS13315 at 2850.232 kb on - strand, within DZA65_RS13315 at 2850.232 kb on - strand, within DZA65_RS13315 at 2850.232 kb on - strand, within DZA65_RS13315 at 2850.232 kb on - strand, within DZA65_RS13315 at 2850.234 kb on + strand, within DZA65_RS13315 at 2850.234 kb on + strand, within DZA65_RS13315 at 2850.234 kb on + strand, within DZA65_RS13315 at 2850.234 kb on + strand, within DZA65_RS13315 at 2850.292 kb on - strand, within DZA65_RS13315 at 2850.292 kb on - strand, within DZA65_RS13315 at 2850.292 kb on - strand, within DZA65_RS13315 at 2850.292 kb on - strand, within DZA65_RS13315 at 2850.378 kb on + strand, within DZA65_RS13315 at 2850.381 kb on + strand, within DZA65_RS13315 at 2850.381 kb on + strand, within DZA65_RS13315 at 2850.381 kb on + strand, within DZA65_RS13315 at 2850.381 kb on + strand, within DZA65_RS13315 at 2850.381 kb on + strand, within DZA65_RS13315 at 2850.382 kb on - strand, within DZA65_RS13315 at 2850.382 kb on - strand, within DZA65_RS13315 at 2850.382 kb on - strand, within DZA65_RS13315 at 2850.413 kb on + strand, within DZA65_RS13315 at 2850.413 kb on + strand, within DZA65_RS13315 at 2850.470 kb on + strand at 2850.471 kb on - strand at 2850.471 kb on - strand at 2850.640 kb on + strand, within DZA65_RS13320 at 2850.673 kb on + strand, within DZA65_RS13320 at 2850.749 kb on + strand, within DZA65_RS13320 at 2850.780 kb on + strand, within DZA65_RS13320 at 2850.781 kb on - strand, within DZA65_RS13320 at 2850.862 kb on + strand, within DZA65_RS13320 at 2850.967 kb on + strand at 2850.967 kb on + strand at 2850.967 kb on + strand at 2850.971 kb on + strand at 2850.971 kb on + strand at 2850.971 kb on + strand at 2850.971 kb on + strand at 2850.972 kb on - strand at 2850.972 kb on - strand at 2850.972 kb on - strand at 2850.972 kb on - strand at 2850.972 kb on - strand at 2850.976 kb on + strand at 2850.976 kb on + strand at 2850.976 kb on + strand at 2850.977 kb on - strand at 2850.977 kb on - strand at 2850.977 kb on - strand at 2850.977 kb on - strand at 2851.071 kb on - strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.133 kb on + strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.134 kb on - strand at 2851.175 kb on + strand at 2851.175 kb on + strand at 2851.175 kb on + strand at 2851.176 kb on - strand at 2851.176 kb on - strand at 2851.176 kb on - strand at 2851.176 kb on - strand at 2851.176 kb on - strand at 2851.176 kb on - strand at 2851.288 kb on - strand at 2851.304 kb on + strand, within DZA65_RS13325 at 2851.305 kb on - strand, within DZA65_RS13325
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,849,099 + -0.9 2,849,099 + -0.6 2,849,099 + -1.9 2,849,099 + -1.4 2,849,099 + -0.9 2,849,099 + -1.4 2,849,100 - -0.8 2,849,100 - -0.9 2,849,101 + -1.9 2,849,102 - -2.9 2,849,145 + -1.9 2,849,146 - -0.4 2,849,146 - -1.4 2,849,146 - -1.7 2,849,146 - +1.2 2,849,146 - -1.0 2,849,240 + DZA65_RS13310 0.17 -2.4 2,849,240 + DZA65_RS13310 0.17 -2.7 2,849,338 - DZA65_RS13310 0.43 -2.2 2,849,378 + DZA65_RS13310 0.54 -0.8 2,849,378 + DZA65_RS13310 0.54 -1.4 2,849,379 - DZA65_RS13310 0.54 -0.4 2,849,379 - DZA65_RS13310 0.54 -1.9 2,849,383 - DZA65_RS13310 0.55 -1.1 2,849,464 + DZA65_RS13310 0.77 +0.6 2,849,465 - DZA65_RS13310 0.77 -2.5 2,849,531 + -0.7 2,849,531 + -2.3 2,849,531 + -1.2 2,849,532 - -2.7 2,849,532 - -1.2 2,849,532 - +0.4 2,849,532 - -1.7 2,849,532 - -0.4 2,849,570 + -1.7 2,849,570 + -1.3 2,849,649 - +0.2 2,849,678 - +0.4 2,849,681 + -0.9 2,849,724 + +1.1 2,849,724 + -0.4 2,849,724 + -0.9 2,849,725 - -0.4 2,849,725 - -0.3 2,849,725 - +0.1 2,849,806 + -2.4 2,849,820 + -1.9 2,849,821 - +0.6 2,849,821 - -1.4 2,849,823 - -0.5 2,849,823 - -0.4 2,849,841 - +0.2 2,849,842 + +0.4 2,849,843 - -2.4 2,849,843 - +0.4 2,849,843 - -0.4 2,849,846 + -0.4 2,849,847 - -0.6 2,849,854 + +0.4 2,849,865 - -1.2 2,849,881 + -0.9 2,849,890 + +0.4 2,849,890 + +1.3 2,849,892 + -0.3 2,849,964 - -1.1 2,849,964 - +0.6 2,850,019 + -0.3 2,850,083 + -0.4 2,850,084 - -0.1 2,850,084 - -0.7 2,850,084 - -1.7 2,850,095 + -0.6 2,850,096 - -0.9 2,850,096 - -0.2 2,850,174 + DZA65_RS13315 0.19 -1.9 2,850,231 + DZA65_RS13315 0.34 -1.4 2,850,231 + DZA65_RS13315 0.34 -0.6 2,850,232 - DZA65_RS13315 0.34 -1.3 2,850,232 - DZA65_RS13315 0.34 +0.3 2,850,232 - DZA65_RS13315 0.34 -0.1 2,850,232 - DZA65_RS13315 0.34 -0.5 2,850,232 - DZA65_RS13315 0.34 -1.1 2,850,234 + DZA65_RS13315 0.34 -1.2 2,850,234 + DZA65_RS13315 0.34 -1.4 2,850,234 + DZA65_RS13315 0.34 -0.2 2,850,234 + DZA65_RS13315 0.34 -0.6 2,850,292 - DZA65_RS13315 0.49 -1.6 2,850,292 - DZA65_RS13315 0.49 -0.7 2,850,292 - DZA65_RS13315 0.49 +0.5 2,850,292 - DZA65_RS13315 0.49 +0.3 2,850,378 + DZA65_RS13315 0.70 -2.0 2,850,381 + DZA65_RS13315 0.71 -0.2 2,850,381 + DZA65_RS13315 0.71 -0.7 2,850,381 + DZA65_RS13315 0.71 +1.6 2,850,381 + DZA65_RS13315 0.71 -0.2 2,850,381 + DZA65_RS13315 0.71 -1.7 2,850,382 - DZA65_RS13315 0.71 -2.5 2,850,382 - DZA65_RS13315 0.71 +1.0 2,850,382 - DZA65_RS13315 0.71 -0.9 2,850,413 + DZA65_RS13315 0.79 -1.9 2,850,413 + DZA65_RS13315 0.79 +0.8 2,850,470 + -1.5 2,850,471 - -1.4 2,850,471 - -0.5 2,850,640 + DZA65_RS13320 0.17 -0.4 2,850,673 + DZA65_RS13320 0.25 -0.6 2,850,749 + DZA65_RS13320 0.43 +1.2 2,850,780 + DZA65_RS13320 0.50 +0.4 2,850,781 - DZA65_RS13320 0.50 +0.2 2,850,862 + DZA65_RS13320 0.69 -1.2 2,850,967 + -0.2 2,850,967 + +0.3 2,850,967 + -1.4 2,850,971 + +1.6 2,850,971 + +0.1 2,850,971 + -0.7 2,850,971 + -0.2 2,850,972 - +0.1 2,850,972 - -2.5 2,850,972 - -0.9 2,850,972 - -0.2 2,850,972 - +0.4 2,850,976 + +0.6 2,850,976 + -1.5 2,850,976 + -1.8 2,850,977 - -0.9 2,850,977 - +0.6 2,850,977 - -1.5 2,850,977 - -1.2 2,851,071 - -2.4 2,851,133 + -0.2 2,851,133 + -0.8 2,851,133 + -0.9 2,851,133 + -1.3 2,851,133 + -0.2 2,851,133 + -2.7 2,851,133 + -0.9 2,851,133 + -0.7 2,851,133 + +0.1 2,851,133 + -0.5 2,851,133 + -0.1 2,851,133 + -0.9 2,851,134 - -0.4 2,851,134 - -1.4 2,851,134 - -1.2 2,851,134 - -2.2 2,851,134 - -0.9 2,851,134 - -1.1 2,851,134 - -0.4 2,851,134 - +0.6 2,851,134 - -0.8 2,851,134 - -1.1 2,851,134 - +0.2 2,851,134 - -1.1 2,851,134 - -1.4 2,851,134 - -1.4 2,851,134 - +1.1 2,851,134 - -0.2 2,851,134 - +1.6 2,851,175 + -0.4 2,851,175 + -0.3 2,851,175 + -1.9 2,851,176 - +1.3 2,851,176 - -0.0 2,851,176 - -1.7 2,851,176 - -0.9 2,851,176 - -0.4 2,851,176 - +2.2 2,851,288 - -1.7 2,851,304 + DZA65_RS13325 0.10 -0.2 2,851,305 - DZA65_RS13325 0.10 +0.2
Or see this region's nucleotide sequence