Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS12440 and DZA65_RS12445 are separated by 280 nucleotides DZA65_RS12445 and DZA65_RS12450 are separated by 71 nucleotides DZA65_RS12450 and DZA65_RS12455 overlap by 1 nucleotides DZA65_RS12455 and DZA65_RS12460 overlap by 4 nucleotides
DZA65_RS12440: DZA65_RS12440 - L-threonine dehydrogenase, at 2,659,312 to 2,660,460
_RS12440
DZA65_RS12445: DZA65_RS12445 - NUDIX domain-containing protein, at 2,660,741 to 2,661,301
_RS12445
DZA65_RS12450: DZA65_RS12450 - respiratory nitrate reductase subunit gamma, at 2,661,373 to 2,662,050
_RS12450
DZA65_RS12455: DZA65_RS12455 - nitrate reductase molybdenum cofactor assembly chaperone, at 2,662,050 to 2,662,769
_RS12455
DZA65_RS12460: DZA65_RS12460 - nitrate reductase subunit beta, at 2,662,766 to 2,664,328
_RS12460
Position (kb)
2661
2662
2663 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2660.407 kb on + strand at 2660.408 kb on - strand at 2660.408 kb on - strand at 2660.444 kb on + strand at 2660.444 kb on + strand at 2660.445 kb on - strand at 2660.445 kb on - strand at 2660.460 kb on + strand at 2660.460 kb on + strand at 2660.460 kb on + strand at 2660.479 kb on + strand at 2660.480 kb on - strand at 2660.494 kb on + strand at 2660.494 kb on + strand at 2660.494 kb on + strand at 2660.495 kb on - strand at 2660.495 kb on - strand at 2660.505 kb on + strand at 2660.505 kb on + strand at 2660.505 kb on + strand at 2660.590 kb on - strand at 2660.599 kb on + strand at 2660.600 kb on - strand at 2660.654 kb on + strand at 2660.654 kb on + strand at 2660.654 kb on + strand at 2660.655 kb on - strand at 2660.681 kb on - strand at 2660.695 kb on + strand at 2660.695 kb on + strand at 2660.696 kb on - strand at 2660.696 kb on - strand at 2660.696 kb on - strand at 2660.716 kb on - strand at 2660.718 kb on + strand at 2660.718 kb on + strand at 2660.718 kb on + strand at 2660.718 kb on + strand at 2660.718 kb on + strand at 2660.718 kb on + strand at 2660.718 kb on + strand at 2660.718 kb on + strand at 2660.718 kb on + strand at 2660.719 kb on - strand at 2660.719 kb on - strand at 2660.719 kb on - strand at 2660.719 kb on - strand at 2660.719 kb on - strand at 2660.719 kb on - strand at 2660.745 kb on + strand at 2660.746 kb on - strand at 2660.746 kb on - strand at 2660.746 kb on - strand at 2660.746 kb on - strand at 2660.746 kb on - strand at 2660.776 kb on - strand at 2660.781 kb on + strand at 2660.781 kb on + strand at 2660.781 kb on + strand at 2660.781 kb on + strand at 2660.781 kb on + strand at 2660.782 kb on - strand at 2660.784 kb on + strand at 2660.784 kb on + strand at 2660.785 kb on - strand at 2660.785 kb on - strand at 2660.795 kb on + strand at 2660.795 kb on + strand at 2660.795 kb on + strand at 2660.795 kb on + strand at 2660.795 kb on + strand at 2660.796 kb on - strand at 2660.807 kb on + strand, within DZA65_RS12445 at 2660.807 kb on + strand, within DZA65_RS12445 at 2660.807 kb on + strand, within DZA65_RS12445 at 2660.808 kb on - strand, within DZA65_RS12445 at 2660.808 kb on - strand, within DZA65_RS12445 at 2660.829 kb on + strand, within DZA65_RS12445 at 2660.829 kb on + strand, within DZA65_RS12445 at 2660.829 kb on + strand, within DZA65_RS12445 at 2660.829 kb on + strand, within DZA65_RS12445 at 2660.829 kb on + strand, within DZA65_RS12445 at 2660.829 kb on + strand, within DZA65_RS12445 at 2660.830 kb on - strand, within DZA65_RS12445 at 2660.830 kb on - strand, within DZA65_RS12445 at 2660.830 kb on - strand, within DZA65_RS12445 at 2660.841 kb on + strand, within DZA65_RS12445 at 2660.841 kb on + strand, within DZA65_RS12445 at 2660.866 kb on + strand, within DZA65_RS12445 at 2660.866 kb on + strand, within DZA65_RS12445 at 2660.867 kb on - strand, within DZA65_RS12445 at 2660.867 kb on - strand, within DZA65_RS12445 at 2660.867 kb on - strand, within DZA65_RS12445 at 2660.867 kb on - strand, within DZA65_RS12445 at 2660.888 kb on - strand, within DZA65_RS12445 at 2660.902 kb on + strand, within DZA65_RS12445 at 2661.057 kb on + strand, within DZA65_RS12445 at 2661.057 kb on + strand, within DZA65_RS12445 at 2661.057 kb on + strand, within DZA65_RS12445 at 2661.057 kb on + strand, within DZA65_RS12445 at 2661.058 kb on - strand, within DZA65_RS12445 at 2661.058 kb on - strand, within DZA65_RS12445 at 2661.096 kb on + strand, within DZA65_RS12445 at 2661.097 kb on - strand, within DZA65_RS12445 at 2661.131 kb on - strand, within DZA65_RS12445 at 2661.146 kb on + strand, within DZA65_RS12445 at 2661.147 kb on - strand, within DZA65_RS12445 at 2661.147 kb on - strand, within DZA65_RS12445 at 2661.148 kb on + strand, within DZA65_RS12445 at 2661.148 kb on + strand, within DZA65_RS12445 at 2661.148 kb on + strand, within DZA65_RS12445 at 2661.148 kb on + strand, within DZA65_RS12445 at 2661.149 kb on - strand, within DZA65_RS12445 at 2661.258 kb on - strand at 2661.279 kb on + strand at 2661.280 kb on - strand at 2661.280 kb on - strand at 2661.366 kb on + strand at 2661.366 kb on + strand at 2661.366 kb on + strand at 2661.366 kb on + strand at 2661.366 kb on + strand at 2661.366 kb on + strand at 2661.366 kb on + strand at 2661.367 kb on - strand at 2661.367 kb on - strand at 2661.367 kb on - strand at 2661.367 kb on - strand at 2661.374 kb on + strand at 2661.375 kb on - strand at 2661.375 kb on - strand at 2661.375 kb on - strand at 2661.413 kb on + strand at 2661.414 kb on - strand at 2661.414 kb on - strand at 2661.414 kb on - strand at 2661.435 kb on + strand at 2661.435 kb on + strand at 2661.435 kb on + strand at 2661.436 kb on - strand at 2661.524 kb on + strand, within DZA65_RS12450 at 2661.600 kb on + strand, within DZA65_RS12450 at 2661.601 kb on - strand, within DZA65_RS12450 at 2661.601 kb on - strand, within DZA65_RS12450 at 2661.821 kb on + strand, within DZA65_RS12450 at 2661.821 kb on + strand, within DZA65_RS12450 at 2661.821 kb on + strand, within DZA65_RS12450 at 2661.821 kb on + strand, within DZA65_RS12450 at 2661.821 kb on + strand, within DZA65_RS12450 at 2661.821 kb on + strand, within DZA65_RS12450 at 2661.822 kb on - strand, within DZA65_RS12450 at 2661.822 kb on - strand, within DZA65_RS12450 at 2661.822 kb on - strand, within DZA65_RS12450 at 2661.822 kb on - strand, within DZA65_RS12450 at 2661.822 kb on - strand, within DZA65_RS12450 at 2661.822 kb on - strand, within DZA65_RS12450 at 2661.954 kb on - strand, within DZA65_RS12450 at 2661.963 kb on - strand, within DZA65_RS12450 at 2661.969 kb on - strand, within DZA65_RS12450 at 2662.010 kb on + strand at 2662.010 kb on + strand at 2662.010 kb on + strand at 2662.010 kb on + strand at 2662.011 kb on - strand at 2662.011 kb on - strand at 2662.011 kb on - strand at 2662.011 kb on - strand at 2662.011 kb on - strand at 2662.016 kb on + strand at 2662.016 kb on + strand at 2662.016 kb on + strand at 2662.016 kb on + strand at 2662.016 kb on + strand at 2662.017 kb on - strand at 2662.017 kb on - strand at 2662.017 kb on - strand at 2662.017 kb on - strand at 2662.017 kb on - strand at 2662.017 kb on - strand at 2662.020 kb on + strand at 2662.020 kb on + strand at 2662.021 kb on - strand at 2662.021 kb on - strand at 2662.043 kb on + strand at 2662.043 kb on + strand at 2662.043 kb on + strand at 2662.044 kb on - strand at 2662.236 kb on + strand, within DZA65_RS12455 at 2662.290 kb on + strand, within DZA65_RS12455 at 2662.290 kb on + strand, within DZA65_RS12455 at 2662.290 kb on + strand, within DZA65_RS12455 at 2662.290 kb on + strand, within DZA65_RS12455 at 2662.291 kb on - strand, within DZA65_RS12455 at 2662.291 kb on - strand, within DZA65_RS12455 at 2662.291 kb on - strand, within DZA65_RS12455 at 2662.310 kb on - strand, within DZA65_RS12455 at 2662.411 kb on + strand, within DZA65_RS12455 at 2662.412 kb on - strand, within DZA65_RS12455 at 2662.412 kb on - strand, within DZA65_RS12455 at 2662.421 kb on - strand, within DZA65_RS12455 at 2662.618 kb on + strand, within DZA65_RS12455 at 2662.733 kb on - strand at 2662.774 kb on + strand at 2662.774 kb on + strand at 2662.774 kb on + strand at 2662.774 kb on + strand at 2662.774 kb on + strand at 2662.775 kb on - strand at 2662.775 kb on - strand at 2662.775 kb on - strand at 2662.775 kb on - strand at 2662.828 kb on + strand at 2662.837 kb on + strand at 2662.977 kb on + strand, within DZA65_RS12460 at 2662.977 kb on + strand at 2662.977 kb on + strand, within DZA65_RS12460 at 2662.977 kb on + strand, within DZA65_RS12460 at 2662.977 kb on + strand, within DZA65_RS12460 at 2662.977 kb on + strand, within DZA65_RS12460 at 2662.978 kb on - strand, within DZA65_RS12460 at 2662.978 kb on - strand, within DZA65_RS12460 at 2662.978 kb on - strand, within DZA65_RS12460 at 2662.978 kb on - strand, within DZA65_RS12460 at 2663.001 kb on + strand, within DZA65_RS12460 at 2663.001 kb on + strand, within DZA65_RS12460 at 2663.001 kb on + strand, within DZA65_RS12460 at 2663.002 kb on - strand, within DZA65_RS12460 at 2663.002 kb on - strand, within DZA65_RS12460 at 2663.002 kb on - strand, within DZA65_RS12460 at 2663.002 kb on - strand, within DZA65_RS12460
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,660,407 + -0.8 2,660,408 - +0.1 2,660,408 - -0.4 2,660,444 + +0.3 2,660,444 + -0.6 2,660,445 - +0.1 2,660,445 - -1.4 2,660,460 + -0.6 2,660,460 + +1.1 2,660,460 + -0.4 2,660,479 + -0.1 2,660,480 - -0.4 2,660,494 + +0.0 2,660,494 + -0.4 2,660,494 + -0.1 2,660,495 - +0.6 2,660,495 - +1.0 2,660,505 + -0.9 2,660,505 + +0.6 2,660,505 + -0.6 2,660,590 - -0.3 2,660,599 + -1.5 2,660,600 - -0.2 2,660,654 + -2.2 2,660,654 + -2.1 2,660,654 + -0.3 2,660,655 - -1.6 2,660,681 - -0.1 2,660,695 + +0.6 2,660,695 + -1.2 2,660,696 - -0.1 2,660,696 - -0.7 2,660,696 - -0.6 2,660,716 - -2.1 2,660,718 + -0.8 2,660,718 + +0.1 2,660,718 + -1.4 2,660,718 + -1.4 2,660,718 + +0.0 2,660,718 + -0.1 2,660,718 + -2.2 2,660,718 + +0.3 2,660,718 + -0.7 2,660,719 - -0.3 2,660,719 - +0.4 2,660,719 - -0.6 2,660,719 - +0.4 2,660,719 - -0.4 2,660,719 - +1.3 2,660,745 + +0.0 2,660,746 - -0.6 2,660,746 - -0.9 2,660,746 - -0.5 2,660,746 - -1.5 2,660,746 - +0.2 2,660,776 - +0.9 2,660,781 + -0.5 2,660,781 + -0.1 2,660,781 + -1.2 2,660,781 + +0.4 2,660,781 + -1.4 2,660,782 - -0.2 2,660,784 + -1.7 2,660,784 + +0.3 2,660,785 - +0.3 2,660,785 - -0.3 2,660,795 + -1.7 2,660,795 + +1.6 2,660,795 + -0.3 2,660,795 + -0.9 2,660,795 + -2.4 2,660,796 - +2.2 2,660,807 + DZA65_RS12445 0.12 -0.4 2,660,807 + DZA65_RS12445 0.12 -1.4 2,660,807 + DZA65_RS12445 0.12 +0.2 2,660,808 - DZA65_RS12445 0.12 +0.3 2,660,808 - DZA65_RS12445 0.12 -0.2 2,660,829 + DZA65_RS12445 0.16 +0.2 2,660,829 + DZA65_RS12445 0.16 +0.3 2,660,829 + DZA65_RS12445 0.16 -0.4 2,660,829 + DZA65_RS12445 0.16 -0.4 2,660,829 + DZA65_RS12445 0.16 +0.3 2,660,829 + DZA65_RS12445 0.16 -1.2 2,660,830 - DZA65_RS12445 0.16 +0.6 2,660,830 - DZA65_RS12445 0.16 -2.0 2,660,830 - DZA65_RS12445 0.16 +0.4 2,660,841 + DZA65_RS12445 0.18 -0.7 2,660,841 + DZA65_RS12445 0.18 -0.4 2,660,866 + DZA65_RS12445 0.22 -0.4 2,660,866 + DZA65_RS12445 0.22 -0.4 2,660,867 - DZA65_RS12445 0.22 -0.3 2,660,867 - DZA65_RS12445 0.22 +0.6 2,660,867 - DZA65_RS12445 0.22 +0.2 2,660,867 - DZA65_RS12445 0.22 -1.3 2,660,888 - DZA65_RS12445 0.26 -0.2 2,660,902 + DZA65_RS12445 0.29 -0.7 2,661,057 + DZA65_RS12445 0.56 -0.1 2,661,057 + DZA65_RS12445 0.56 -0.5 2,661,057 + DZA65_RS12445 0.56 -1.2 2,661,057 + DZA65_RS12445 0.56 +0.0 2,661,058 - DZA65_RS12445 0.57 -0.6 2,661,058 - DZA65_RS12445 0.57 -0.4 2,661,096 + DZA65_RS12445 0.63 -1.2 2,661,097 - DZA65_RS12445 0.63 -0.1 2,661,131 - DZA65_RS12445 0.70 +0.4 2,661,146 + DZA65_RS12445 0.72 -2.0 2,661,147 - DZA65_RS12445 0.72 -0.4 2,661,147 - DZA65_RS12445 0.72 -1.2 2,661,148 + DZA65_RS12445 0.73 -0.5 2,661,148 + DZA65_RS12445 0.73 -1.0 2,661,148 + DZA65_RS12445 0.73 -0.4 2,661,148 + DZA65_RS12445 0.73 +0.4 2,661,149 - DZA65_RS12445 0.73 -1.4 2,661,258 - +0.2 2,661,279 + -1.2 2,661,280 - +0.6 2,661,280 - -0.3 2,661,366 + -0.4 2,661,366 + -1.4 2,661,366 + +1.6 2,661,366 + -0.1 2,661,366 + -2.4 2,661,366 + -1.7 2,661,366 + -1.1 2,661,367 - -0.0 2,661,367 - -0.2 2,661,367 - -1.0 2,661,367 - -0.6 2,661,374 + +0.8 2,661,375 - +0.8 2,661,375 - +0.9 2,661,375 - -2.2 2,661,413 + -0.7 2,661,414 - -1.2 2,661,414 - +0.5 2,661,414 - +0.6 2,661,435 + -0.7 2,661,435 + -2.1 2,661,435 + -0.5 2,661,436 - +0.1 2,661,524 + DZA65_RS12450 0.22 -1.7 2,661,600 + DZA65_RS12450 0.33 +0.6 2,661,601 - DZA65_RS12450 0.34 +0.3 2,661,601 - DZA65_RS12450 0.34 +1.5 2,661,821 + DZA65_RS12450 0.66 -1.2 2,661,821 + DZA65_RS12450 0.66 +0.1 2,661,821 + DZA65_RS12450 0.66 +0.8 2,661,821 + DZA65_RS12450 0.66 -0.6 2,661,821 + DZA65_RS12450 0.66 -1.2 2,661,821 + DZA65_RS12450 0.66 +1.4 2,661,822 - DZA65_RS12450 0.66 -1.0 2,661,822 - DZA65_RS12450 0.66 -2.1 2,661,822 - DZA65_RS12450 0.66 +1.2 2,661,822 - DZA65_RS12450 0.66 +0.5 2,661,822 - DZA65_RS12450 0.66 -0.3 2,661,822 - DZA65_RS12450 0.66 +0.1 2,661,954 - DZA65_RS12450 0.86 +0.2 2,661,963 - DZA65_RS12450 0.87 -0.7 2,661,969 - DZA65_RS12450 0.88 +0.9 2,662,010 + +0.9 2,662,010 + -0.7 2,662,010 + -0.4 2,662,010 + -0.4 2,662,011 - +0.2 2,662,011 - +2.4 2,662,011 - -3.0 2,662,011 - -0.7 2,662,011 - +1.4 2,662,016 + +0.6 2,662,016 + -1.9 2,662,016 + +0.2 2,662,016 + -1.0 2,662,016 + +0.1 2,662,017 - -0.6 2,662,017 - +0.2 2,662,017 - +0.2 2,662,017 - -0.3 2,662,017 - -1.1 2,662,017 - -0.9 2,662,020 + +1.2 2,662,020 + -0.4 2,662,021 - -0.4 2,662,021 - +0.4 2,662,043 + -1.0 2,662,043 + +0.6 2,662,043 + +0.1 2,662,044 - +0.3 2,662,236 + DZA65_RS12455 0.26 +1.3 2,662,290 + DZA65_RS12455 0.33 -0.6 2,662,290 + DZA65_RS12455 0.33 -0.1 2,662,290 + DZA65_RS12455 0.33 +0.6 2,662,290 + DZA65_RS12455 0.33 -0.4 2,662,291 - DZA65_RS12455 0.33 +0.6 2,662,291 - DZA65_RS12455 0.33 -0.7 2,662,291 - DZA65_RS12455 0.33 -1.7 2,662,310 - DZA65_RS12455 0.36 -1.0 2,662,411 + DZA65_RS12455 0.50 +0.6 2,662,412 - DZA65_RS12455 0.50 -1.2 2,662,412 - DZA65_RS12455 0.50 -2.2 2,662,421 - DZA65_RS12455 0.52 +0.1 2,662,618 + DZA65_RS12455 0.79 -0.5 2,662,733 - -0.9 2,662,774 + -0.8 2,662,774 + +0.2 2,662,774 + -0.0 2,662,774 + -0.2 2,662,774 + -1.0 2,662,775 - -2.6 2,662,775 - -0.4 2,662,775 - -0.9 2,662,775 - -2.3 2,662,828 + -0.3 2,662,837 + -0.6 2,662,977 + DZA65_RS12460 0.13 -0.4 2,662,977 + +1.6 2,662,977 + DZA65_RS12460 0.13 +0.6 2,662,977 + DZA65_RS12460 0.13 +0.1 2,662,977 + DZA65_RS12460 0.13 -0.4 2,662,977 + DZA65_RS12460 0.13 -1.1 2,662,978 - DZA65_RS12460 0.14 -0.6 2,662,978 - DZA65_RS12460 0.14 -0.3 2,662,978 - DZA65_RS12460 0.14 -0.5 2,662,978 - DZA65_RS12460 0.14 -0.1 2,663,001 + DZA65_RS12460 0.15 +0.2 2,663,001 + DZA65_RS12460 0.15 -0.6 2,663,001 + DZA65_RS12460 0.15 -1.3 2,663,002 - DZA65_RS12460 0.15 -0.2 2,663,002 - DZA65_RS12460 0.15 -2.2 2,663,002 - DZA65_RS12460 0.15 -2.0 2,663,002 - DZA65_RS12460 0.15 -1.2
Or see this region's nucleotide sequence