Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS11420 and DZA65_RS11425 are separated by 330 nucleotides DZA65_RS11425 and DZA65_RS11430 are separated by 242 nucleotides DZA65_RS11430 and DZA65_RS11435 are separated by 129 nucleotides
DZA65_RS11420: DZA65_RS11420 - ribose-phosphate diphosphokinase, at 2,429,661 to 2,430,608
_RS11420
DZA65_RS11425: DZA65_RS11425 - stress-induced protein YchH, at 2,430,939 to 2,431,214
_RS11425
DZA65_RS11430: DZA65_RS11430 - aminoacyl-tRNA hydrolase, at 2,431,457 to 2,432,044
_RS11430
DZA65_RS11435: DZA65_RS11435 - redox-regulated ATPase YchF, at 2,432,174 to 2,433,265
_RS11435
Position (kb)
2430
2431
2432 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2430.606 kb on + strand at 2430.628 kb on + strand at 2430.629 kb on - strand at 2430.629 kb on - strand at 2430.677 kb on + strand at 2430.694 kb on + strand at 2430.695 kb on - strand at 2430.695 kb on - strand at 2430.705 kb on + strand at 2430.706 kb on - strand at 2430.712 kb on - strand at 2430.736 kb on + strand at 2430.753 kb on - strand at 2430.782 kb on - strand at 2430.798 kb on + strand at 2430.834 kb on + strand at 2430.834 kb on + strand at 2430.834 kb on + strand at 2430.844 kb on - strand at 2430.845 kb on + strand at 2430.846 kb on - strand at 2430.863 kb on - strand at 2430.868 kb on + strand at 2430.868 kb on + strand at 2430.877 kb on + strand at 2430.885 kb on + strand at 2430.886 kb on - strand at 2430.903 kb on - strand at 2430.904 kb on + strand at 2430.914 kb on - strand at 2430.919 kb on + strand at 2430.920 kb on - strand at 2430.997 kb on + strand, within DZA65_RS11425 at 2430.998 kb on - strand, within DZA65_RS11425 at 2430.998 kb on - strand, within DZA65_RS11425 at 2431.025 kb on + strand, within DZA65_RS11425 at 2431.028 kb on + strand, within DZA65_RS11425 at 2431.067 kb on + strand, within DZA65_RS11425 at 2431.085 kb on - strand, within DZA65_RS11425 at 2431.085 kb on - strand, within DZA65_RS11425 at 2431.085 kb on - strand, within DZA65_RS11425 at 2431.085 kb on - strand, within DZA65_RS11425 at 2431.154 kb on + strand, within DZA65_RS11425 at 2431.164 kb on - strand, within DZA65_RS11425 at 2431.168 kb on - strand, within DZA65_RS11425 at 2431.179 kb on + strand, within DZA65_RS11425 at 2431.245 kb on - strand at 2431.245 kb on - strand at 2431.264 kb on - strand at 2431.285 kb on + strand at 2431.285 kb on + strand at 2431.285 kb on + strand at 2431.352 kb on - strand at 2431.352 kb on - strand at 2431.352 kb on - strand at 2431.352 kb on - strand at 2431.417 kb on + strand at 2431.417 kb on + strand at 2432.043 kb on - strand at 2432.078 kb on + strand at 2432.078 kb on + strand at 2432.078 kb on + strand at 2432.078 kb on + strand at 2432.084 kb on + strand at 2432.085 kb on - strand at 2432.085 kb on - strand at 2432.086 kb on + strand at 2432.086 kb on + strand at 2432.087 kb on - strand at 2432.087 kb on - strand at 2432.087 kb on - strand at 2432.087 kb on - strand at 2432.149 kb on + strand at 2432.150 kb on - strand at 2432.159 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,430,606 + +0.5 2,430,628 + -1.0 2,430,629 - -1.6 2,430,629 - +0.5 2,430,677 + +1.1 2,430,694 + +0.1 2,430,695 - -0.5 2,430,695 - -0.9 2,430,705 + -0.9 2,430,706 - -1.2 2,430,712 - -0.3 2,430,736 + +1.0 2,430,753 - +0.5 2,430,782 - +0.1 2,430,798 + -0.2 2,430,834 + +0.4 2,430,834 + -0.1 2,430,834 + -2.9 2,430,844 - +0.2 2,430,845 + +0.4 2,430,846 - -0.3 2,430,863 - -1.8 2,430,868 + -0.2 2,430,868 + +1.3 2,430,877 + -0.1 2,430,885 + -0.5 2,430,886 - -1.2 2,430,903 - -0.2 2,430,904 + -0.2 2,430,914 - +0.3 2,430,919 + -0.8 2,430,920 - +0.1 2,430,997 + DZA65_RS11425 0.21 -0.2 2,430,998 - DZA65_RS11425 0.21 +0.4 2,430,998 - DZA65_RS11425 0.21 -1.3 2,431,025 + DZA65_RS11425 0.31 -0.2 2,431,028 + DZA65_RS11425 0.32 +0.5 2,431,067 + DZA65_RS11425 0.46 +0.1 2,431,085 - DZA65_RS11425 0.53 -0.2 2,431,085 - DZA65_RS11425 0.53 +0.3 2,431,085 - DZA65_RS11425 0.53 +0.8 2,431,085 - DZA65_RS11425 0.53 +0.6 2,431,154 + DZA65_RS11425 0.78 +0.1 2,431,164 - DZA65_RS11425 0.82 -0.1 2,431,168 - DZA65_RS11425 0.83 -0.4 2,431,179 + DZA65_RS11425 0.87 -0.3 2,431,245 - -2.1 2,431,245 - +0.2 2,431,264 - -0.5 2,431,285 + -0.1 2,431,285 + +0.4 2,431,285 + -1.5 2,431,352 - -1.1 2,431,352 - -0.7 2,431,352 - +0.3 2,431,352 - +0.8 2,431,417 + -0.9 2,431,417 + +0.7 2,432,043 - -0.1 2,432,078 + +0.5 2,432,078 + -0.5 2,432,078 + +0.1 2,432,078 + -0.3 2,432,084 + +0.5 2,432,085 - +0.3 2,432,085 - -0.1 2,432,086 + -0.5 2,432,086 + -0.9 2,432,087 - -0.0 2,432,087 - +0.2 2,432,087 - -0.3 2,432,087 - -0.9 2,432,149 + +0.8 2,432,150 - -0.7 2,432,159 + +0.0
Or see this region's nucleotide sequence