Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10245 and DZA65_RS10250 are separated by 380 nucleotides DZA65_RS10250 and DZA65_RS10255 are separated by 37 nucleotides DZA65_RS10255 and DZA65_RS10260 are separated by 70 nucleotides
DZA65_RS10245: DZA65_RS10245 - hypothetical protein, at 2,169,548 to 2,170,150
_RS10245
DZA65_RS10250: DZA65_RS10250 - multicopper oxidase CueO, at 2,170,531 to 2,172,198
_RS10250
DZA65_RS10255: DZA65_RS10255 - helix-turn-helix transcriptional regulator, at 2,172,236 to 2,172,670
_RS10255
DZA65_RS10260: DZA65_RS10260 - NADH:flavin oxidoreductase, at 2,172,741 to 2,173,853
_RS10260
Position (kb)
2170
2171
2172
2173 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2169.587 kb on + strand at 2169.588 kb on - strand at 2169.588 kb on - strand at 2169.654 kb on + strand, within DZA65_RS10245 at 2169.722 kb on + strand, within DZA65_RS10245 at 2169.723 kb on - strand, within DZA65_RS10245 at 2169.723 kb on - strand, within DZA65_RS10245 at 2169.736 kb on + strand, within DZA65_RS10245 at 2169.736 kb on + strand, within DZA65_RS10245 at 2169.737 kb on - strand, within DZA65_RS10245 at 2169.900 kb on + strand, within DZA65_RS10245 at 2169.900 kb on + strand, within DZA65_RS10245 at 2169.934 kb on + strand, within DZA65_RS10245 at 2169.935 kb on - strand, within DZA65_RS10245 at 2169.980 kb on - strand, within DZA65_RS10245 at 2169.997 kb on + strand, within DZA65_RS10245 at 2169.998 kb on - strand, within DZA65_RS10245 at 2170.173 kb on - strand at 2170.202 kb on + strand at 2170.330 kb on - strand at 2170.403 kb on - strand at 2170.422 kb on - strand at 2170.438 kb on + strand at 2170.438 kb on + strand at 2170.439 kb on - strand at 2170.439 kb on - strand at 2170.484 kb on + strand at 2170.487 kb on + strand at 2170.528 kb on + strand at 2170.528 kb on + strand at 2170.528 kb on + strand at 2170.528 kb on + strand at 2170.528 kb on + strand at 2170.528 kb on + strand at 2170.528 kb on + strand at 2170.528 kb on + strand at 2170.528 kb on + strand at 2170.528 kb on + strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.529 kb on - strand at 2170.530 kb on + strand at 2170.530 kb on + strand at 2170.530 kb on + strand at 2170.530 kb on + strand at 2170.530 kb on + strand at 2170.530 kb on + strand at 2170.530 kb on + strand at 2170.530 kb on + strand at 2170.530 kb on + strand at 2170.530 kb on + strand at 2170.531 kb on - strand at 2170.531 kb on - strand at 2170.531 kb on - strand at 2170.531 kb on - strand at 2170.531 kb on - strand at 2170.531 kb on - strand at 2170.531 kb on - strand at 2170.548 kb on + strand at 2170.549 kb on - strand at 2170.563 kb on + strand at 2170.563 kb on + strand at 2170.563 kb on + strand at 2170.564 kb on - strand at 2170.564 kb on - strand at 2170.810 kb on - strand, within DZA65_RS10250 at 2170.963 kb on - strand, within DZA65_RS10250 at 2170.963 kb on - strand, within DZA65_RS10250 at 2170.963 kb on - strand, within DZA65_RS10250 at 2171.073 kb on + strand, within DZA65_RS10250 at 2171.073 kb on + strand, within DZA65_RS10250 at 2171.074 kb on - strand, within DZA65_RS10250 at 2171.250 kb on + strand, within DZA65_RS10250 at 2171.296 kb on + strand, within DZA65_RS10250 at 2171.296 kb on + strand, within DZA65_RS10250 at 2171.297 kb on - strand, within DZA65_RS10250 at 2171.297 kb on - strand, within DZA65_RS10250 at 2171.297 kb on - strand, within DZA65_RS10250 at 2171.297 kb on - strand, within DZA65_RS10250 at 2171.297 kb on - strand, within DZA65_RS10250 at 2171.297 kb on - strand, within DZA65_RS10250 at 2171.297 kb on - strand, within DZA65_RS10250 at 2171.337 kb on - strand, within DZA65_RS10250 at 2171.415 kb on + strand, within DZA65_RS10250 at 2171.416 kb on - strand, within DZA65_RS10250 at 2171.480 kb on + strand, within DZA65_RS10250 at 2171.591 kb on + strand, within DZA65_RS10250 at 2171.645 kb on - strand, within DZA65_RS10250 at 2171.645 kb on - strand, within DZA65_RS10250 at 2171.645 kb on - strand, within DZA65_RS10250 at 2171.645 kb on - strand, within DZA65_RS10250 at 2171.650 kb on - strand, within DZA65_RS10250 at 2171.650 kb on - strand, within DZA65_RS10250 at 2171.650 kb on - strand, within DZA65_RS10250 at 2171.650 kb on - strand, within DZA65_RS10250 at 2171.650 kb on - strand, within DZA65_RS10250 at 2171.673 kb on + strand, within DZA65_RS10250 at 2171.673 kb on + strand, within DZA65_RS10250 at 2171.673 kb on + strand, within DZA65_RS10250 at 2171.673 kb on + strand, within DZA65_RS10250 at 2171.674 kb on - strand, within DZA65_RS10250 at 2171.674 kb on - strand, within DZA65_RS10250 at 2171.674 kb on - strand, within DZA65_RS10250 at 2171.683 kb on - strand, within DZA65_RS10250 at 2171.688 kb on + strand, within DZA65_RS10250 at 2171.853 kb on + strand, within DZA65_RS10250 at 2171.937 kb on - strand, within DZA65_RS10250 at 2171.983 kb on - strand, within DZA65_RS10250 at 2171.983 kb on - strand, within DZA65_RS10250 at 2171.983 kb on - strand, within DZA65_RS10250 at 2172.238 kb on - strand at 2172.238 kb on - strand at 2172.308 kb on + strand, within DZA65_RS10255 at 2172.309 kb on - strand, within DZA65_RS10255 at 2172.309 kb on - strand, within DZA65_RS10255 at 2172.322 kb on - strand, within DZA65_RS10255 at 2172.322 kb on - strand, within DZA65_RS10255 at 2172.390 kb on + strand, within DZA65_RS10255 at 2172.390 kb on + strand, within DZA65_RS10255 at 2172.390 kb on + strand, within DZA65_RS10255 at 2172.390 kb on + strand, within DZA65_RS10255 at 2172.391 kb on - strand, within DZA65_RS10255 at 2172.431 kb on + strand, within DZA65_RS10255 at 2172.431 kb on + strand, within DZA65_RS10255 at 2172.431 kb on + strand, within DZA65_RS10255 at 2172.431 kb on + strand, within DZA65_RS10255 at 2172.432 kb on - strand, within DZA65_RS10255 at 2172.485 kb on - strand, within DZA65_RS10255 at 2172.485 kb on - strand, within DZA65_RS10255 at 2172.487 kb on + strand, within DZA65_RS10255 at 2172.487 kb on + strand, within DZA65_RS10255 at 2172.487 kb on + strand, within DZA65_RS10255 at 2172.487 kb on + strand, within DZA65_RS10255 at 2172.499 kb on + strand, within DZA65_RS10255 at 2172.499 kb on + strand, within DZA65_RS10255 at 2172.500 kb on - strand, within DZA65_RS10255 at 2172.500 kb on - strand, within DZA65_RS10255 at 2172.549 kb on - strand, within DZA65_RS10255 at 2172.566 kb on - strand, within DZA65_RS10255 at 2172.654 kb on - strand at 2172.659 kb on - strand at 2172.673 kb on - strand at 2172.703 kb on + strand at 2172.703 kb on + strand at 2172.704 kb on - strand at 2172.704 kb on - strand at 2172.704 kb on - strand at 2172.704 kb on - strand at 2172.773 kb on + strand at 2172.794 kb on - strand at 2172.803 kb on + strand at 2172.804 kb on - strand at 2172.845 kb on + strand at 2172.870 kb on + strand, within DZA65_RS10260 at 2172.870 kb on + strand, within DZA65_RS10260 at 2172.870 kb on + strand, within DZA65_RS10260 at 2172.874 kb on - strand, within DZA65_RS10260 at 2173.051 kb on + strand, within DZA65_RS10260 at 2173.101 kb on + strand, within DZA65_RS10260 at 2173.101 kb on + strand, within DZA65_RS10260 at 2173.102 kb on - strand, within DZA65_RS10260
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,169,587 + -0.0 2,169,588 - -0.3 2,169,588 - +0.6 2,169,654 + DZA65_RS10245 0.18 -0.4 2,169,722 + DZA65_RS10245 0.29 -0.8 2,169,723 - DZA65_RS10245 0.29 +0.0 2,169,723 - DZA65_RS10245 0.29 +0.4 2,169,736 + DZA65_RS10245 0.31 -1.3 2,169,736 + DZA65_RS10245 0.31 -1.2 2,169,737 - DZA65_RS10245 0.31 -0.6 2,169,900 + DZA65_RS10245 0.58 -1.5 2,169,900 + DZA65_RS10245 0.58 -1.2 2,169,934 + DZA65_RS10245 0.64 +0.3 2,169,935 - DZA65_RS10245 0.64 -1.4 2,169,980 - DZA65_RS10245 0.72 -0.2 2,169,997 + DZA65_RS10245 0.74 +1.2 2,169,998 - DZA65_RS10245 0.75 -0.3 2,170,173 - +0.2 2,170,202 + -0.5 2,170,330 - -0.2 2,170,403 - -0.1 2,170,422 - -1.1 2,170,438 + +0.6 2,170,438 + -0.4 2,170,439 - +0.3 2,170,439 - +0.6 2,170,484 + +1.4 2,170,487 + +1.1 2,170,528 + -0.8 2,170,528 + +0.3 2,170,528 + -2.0 2,170,528 + +1.2 2,170,528 + -0.7 2,170,528 + +1.4 2,170,528 + -0.4 2,170,528 + -1.0 2,170,528 + +0.9 2,170,528 + -2.0 2,170,529 - -0.5 2,170,529 - -0.8 2,170,529 - +0.0 2,170,529 - -1.0 2,170,529 - +0.0 2,170,529 - +0.0 2,170,529 - -1.1 2,170,529 - -0.2 2,170,529 - -0.5 2,170,529 - -1.1 2,170,529 - -1.0 2,170,529 - -0.4 2,170,529 - +1.5 2,170,529 - +0.4 2,170,529 - -0.4 2,170,530 + -1.2 2,170,530 + -0.1 2,170,530 + -0.2 2,170,530 + -0.1 2,170,530 + -0.4 2,170,530 + -0.6 2,170,530 + -0.5 2,170,530 + +0.3 2,170,530 + -1.7 2,170,530 + -1.3 2,170,531 - -0.8 2,170,531 - -1.3 2,170,531 - +0.2 2,170,531 - +0.0 2,170,531 - +0.0 2,170,531 - -0.8 2,170,531 - -0.2 2,170,548 + +0.9 2,170,549 - -1.1 2,170,563 + -2.0 2,170,563 + +1.2 2,170,563 + -2.6 2,170,564 - +0.6 2,170,564 - +1.3 2,170,810 - DZA65_RS10250 0.17 +0.4 2,170,963 - DZA65_RS10250 0.26 +0.8 2,170,963 - DZA65_RS10250 0.26 -0.8 2,170,963 - DZA65_RS10250 0.26 +0.4 2,171,073 + DZA65_RS10250 0.32 +0.4 2,171,073 + DZA65_RS10250 0.32 -1.3 2,171,074 - DZA65_RS10250 0.33 -1.0 2,171,250 + DZA65_RS10250 0.43 +0.3 2,171,296 + DZA65_RS10250 0.46 +1.6 2,171,296 + DZA65_RS10250 0.46 -0.1 2,171,297 - DZA65_RS10250 0.46 -0.5 2,171,297 - DZA65_RS10250 0.46 +1.2 2,171,297 - DZA65_RS10250 0.46 -1.0 2,171,297 - DZA65_RS10250 0.46 +0.5 2,171,297 - DZA65_RS10250 0.46 +0.6 2,171,297 - DZA65_RS10250 0.46 +1.2 2,171,297 - DZA65_RS10250 0.46 -0.1 2,171,337 - DZA65_RS10250 0.48 +0.9 2,171,415 + DZA65_RS10250 0.53 -0.1 2,171,416 - DZA65_RS10250 0.53 -0.9 2,171,480 + DZA65_RS10250 0.57 +0.9 2,171,591 + DZA65_RS10250 0.64 -0.0 2,171,645 - DZA65_RS10250 0.67 -0.6 2,171,645 - DZA65_RS10250 0.67 -1.1 2,171,645 - DZA65_RS10250 0.67 +0.0 2,171,645 - DZA65_RS10250 0.67 +0.6 2,171,650 - DZA65_RS10250 0.67 -0.2 2,171,650 - DZA65_RS10250 0.67 -0.9 2,171,650 - DZA65_RS10250 0.67 -0.0 2,171,650 - DZA65_RS10250 0.67 +0.4 2,171,650 - DZA65_RS10250 0.67 -0.3 2,171,673 + DZA65_RS10250 0.68 -0.8 2,171,673 + DZA65_RS10250 0.68 +1.1 2,171,673 + DZA65_RS10250 0.68 +1.2 2,171,673 + DZA65_RS10250 0.68 -0.6 2,171,674 - DZA65_RS10250 0.69 +0.1 2,171,674 - DZA65_RS10250 0.69 -2.0 2,171,674 - DZA65_RS10250 0.69 -0.2 2,171,683 - DZA65_RS10250 0.69 -0.7 2,171,688 + DZA65_RS10250 0.69 -0.4 2,171,853 + DZA65_RS10250 0.79 -0.1 2,171,937 - DZA65_RS10250 0.84 -1.6 2,171,983 - DZA65_RS10250 0.87 -0.5 2,171,983 - DZA65_RS10250 0.87 -0.5 2,171,983 - DZA65_RS10250 0.87 -0.2 2,172,238 - -0.6 2,172,238 - +0.0 2,172,308 + DZA65_RS10255 0.17 -0.1 2,172,309 - DZA65_RS10255 0.17 -0.1 2,172,309 - DZA65_RS10255 0.17 +0.6 2,172,322 - DZA65_RS10255 0.20 +1.8 2,172,322 - DZA65_RS10255 0.20 -0.5 2,172,390 + DZA65_RS10255 0.35 -0.1 2,172,390 + DZA65_RS10255 0.35 +0.4 2,172,390 + DZA65_RS10255 0.35 +0.6 2,172,390 + DZA65_RS10255 0.35 -2.0 2,172,391 - DZA65_RS10255 0.36 -0.7 2,172,431 + DZA65_RS10255 0.45 -1.3 2,172,431 + DZA65_RS10255 0.45 -0.5 2,172,431 + DZA65_RS10255 0.45 +0.4 2,172,431 + DZA65_RS10255 0.45 +2.8 2,172,432 - DZA65_RS10255 0.45 +0.9 2,172,485 - DZA65_RS10255 0.57 -0.2 2,172,485 - DZA65_RS10255 0.57 +0.6 2,172,487 + DZA65_RS10255 0.58 +0.1 2,172,487 + DZA65_RS10255 0.58 -0.4 2,172,487 + DZA65_RS10255 0.58 -0.4 2,172,487 + DZA65_RS10255 0.58 -0.5 2,172,499 + DZA65_RS10255 0.60 -0.7 2,172,499 + DZA65_RS10255 0.60 -2.0 2,172,500 - DZA65_RS10255 0.61 -1.4 2,172,500 - DZA65_RS10255 0.61 -0.6 2,172,549 - DZA65_RS10255 0.72 -1.7 2,172,566 - DZA65_RS10255 0.76 +1.4 2,172,654 - +0.1 2,172,659 - +0.4 2,172,673 - -1.0 2,172,703 + +0.5 2,172,703 + -1.3 2,172,704 - +0.3 2,172,704 - +0.3 2,172,704 - -0.8 2,172,704 - +0.2 2,172,773 + -0.2 2,172,794 - +1.0 2,172,803 + +0.1 2,172,804 - +1.0 2,172,845 + +0.6 2,172,870 + DZA65_RS10260 0.12 -0.5 2,172,870 + DZA65_RS10260 0.12 +1.0 2,172,870 + DZA65_RS10260 0.12 +0.1 2,172,874 - DZA65_RS10260 0.12 -0.5 2,173,051 + DZA65_RS10260 0.28 +0.2 2,173,101 + DZA65_RS10260 0.32 -1.0 2,173,101 + DZA65_RS10260 0.32 -0.2 2,173,102 - DZA65_RS10260 0.32 -1.7
Or see this region's nucleotide sequence