Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS05930 and DZA65_RS05935 are separated by 65 nucleotides DZA65_RS05935 and DZA65_RS05940 overlap by 38 nucleotides DZA65_RS05940 and DZA65_RS05945 are separated by 172 nucleotides
DZA65_RS05930: DZA65_RS05930 - tRNA 4-thiouridine(8) synthase ThiI, at 1,222,726 to 1,224,174
_RS05930
DZA65_RS05935: DZA65_RS05935 - protein deglycase YajL, at 1,224,240 to 1,224,839
_RS05935
DZA65_RS05940: DZA65_RS05940 - 2-dehydropantoate 2-reductase, at 1,224,802 to 1,225,722
_RS05940
DZA65_RS05945: DZA65_RS05945 - YajQ family cyclic di-GMP-binding protein, at 1,225,895 to 1,226,386
_RS05945
Position (kb)
1224
1225
1226 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1224.034 kb on + strand at 1224.080 kb on - strand at 1224.127 kb on + strand at 1224.150 kb on + strand at 1224.164 kb on - strand at 1224.191 kb on + strand at 1224.191 kb on + strand at 1224.192 kb on - strand at 1224.192 kb on - strand at 1224.192 kb on - strand at 1224.192 kb on - strand at 1224.257 kb on - strand at 1224.259 kb on + strand at 1224.259 kb on + strand at 1224.259 kb on + strand at 1224.259 kb on + strand at 1224.259 kb on + strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.260 kb on - strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.265 kb on + strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.266 kb on - strand at 1224.269 kb on + strand at 1224.269 kb on + strand at 1224.270 kb on - strand at 1224.470 kb on + strand, within DZA65_RS05935 at 1224.471 kb on - strand, within DZA65_RS05935 at 1224.471 kb on - strand, within DZA65_RS05935 at 1224.471 kb on - strand, within DZA65_RS05935 at 1224.487 kb on - strand, within DZA65_RS05935 at 1224.687 kb on + strand, within DZA65_RS05935 at 1224.839 kb on + strand at 1224.839 kb on + strand at 1224.859 kb on - strand at 1224.861 kb on + strand at 1224.861 kb on + strand at 1224.861 kb on + strand at 1224.861 kb on + strand at 1224.861 kb on + strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.862 kb on - strand at 1224.863 kb on + strand at 1224.864 kb on - strand at 1224.864 kb on - strand at 1224.864 kb on - strand at 1224.864 kb on - strand at 1224.927 kb on - strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.936 kb on + strand, within DZA65_RS05940 at 1224.937 kb on - strand, within DZA65_RS05940 at 1224.937 kb on - strand, within DZA65_RS05940 at 1224.937 kb on - strand, within DZA65_RS05940 at 1224.937 kb on - strand, within DZA65_RS05940 at 1224.937 kb on - strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.938 kb on + strand, within DZA65_RS05940 at 1224.939 kb on - strand, within DZA65_RS05940 at 1224.939 kb on - strand, within DZA65_RS05940 at 1224.939 kb on - strand, within DZA65_RS05940 at 1224.939 kb on - strand, within DZA65_RS05940 at 1224.991 kb on - strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.025 kb on + strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.026 kb on - strand, within DZA65_RS05940 at 1225.050 kb on + strand, within DZA65_RS05940 at 1225.164 kb on + strand, within DZA65_RS05940 at 1225.164 kb on + strand, within DZA65_RS05940 at 1225.216 kb on - strand, within DZA65_RS05940 at 1225.302 kb on + strand, within DZA65_RS05940 at 1225.303 kb on - strand, within DZA65_RS05940 at 1225.441 kb on + strand, within DZA65_RS05940 at 1225.441 kb on + strand, within DZA65_RS05940 at 1225.441 kb on + strand, within DZA65_RS05940 at 1225.441 kb on + strand, within DZA65_RS05940 at 1225.442 kb on - strand, within DZA65_RS05940 at 1225.488 kb on + strand, within DZA65_RS05940 at 1225.495 kb on + strand, within DZA65_RS05940 at 1225.496 kb on - strand, within DZA65_RS05940 at 1225.569 kb on + strand, within DZA65_RS05940 at 1225.569 kb on + strand, within DZA65_RS05940 at 1225.569 kb on + strand, within DZA65_RS05940 at 1225.569 kb on + strand, within DZA65_RS05940 at 1225.575 kb on + strand, within DZA65_RS05940 at 1225.575 kb on + strand, within DZA65_RS05940 at 1225.575 kb on + strand, within DZA65_RS05940 at 1225.575 kb on + strand, within DZA65_RS05940 at 1225.576 kb on - strand, within DZA65_RS05940 at 1225.576 kb on - strand, within DZA65_RS05940 at 1225.576 kb on - strand, within DZA65_RS05940 at 1225.599 kb on + strand, within DZA65_RS05940 at 1225.600 kb on - strand, within DZA65_RS05940 at 1225.630 kb on - strand, within DZA65_RS05940 at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.645 kb on + strand at 1225.646 kb on - strand at 1225.666 kb on + strand at 1225.667 kb on - strand at 1225.667 kb on - strand at 1225.769 kb on + strand at 1225.769 kb on + strand at 1225.769 kb on + strand at 1225.770 kb on - strand at 1225.770 kb on - strand at 1225.770 kb on - strand at 1225.774 kb on + strand at 1225.775 kb on - strand at 1225.779 kb on + strand at 1225.779 kb on + strand at 1225.779 kb on + strand at 1225.779 kb on + strand at 1225.779 kb on + strand at 1225.842 kb on + strand at 1225.842 kb on + strand at 1225.843 kb on - strand at 1225.843 kb on - strand at 1225.910 kb on - strand at 1225.943 kb on - strand at 1225.993 kb on + strand, within DZA65_RS05945 at 1225.994 kb on - strand, within DZA65_RS05945 at 1226.035 kb on + strand, within DZA65_RS05945 at 1226.036 kb on - strand, within DZA65_RS05945 at 1226.036 kb on - strand, within DZA65_RS05945 at 1226.168 kb on + strand, within DZA65_RS05945 at 1226.169 kb on - strand, within DZA65_RS05945 at 1226.322 kb on + strand, within DZA65_RS05945 at 1226.322 kb on + strand, within DZA65_RS05945 at 1226.323 kb on - strand, within DZA65_RS05945 at 1226.371 kb on + strand at 1226.394 kb on + strand at 1226.394 kb on + strand at 1226.436 kb on + strand at 1226.466 kb on + strand at 1226.467 kb on - strand at 1226.467 kb on - strand at 1226.539 kb on - strand at 1226.620 kb on + strand at 1226.620 kb on + strand at 1226.620 kb on + strand at 1226.621 kb on - strand at 1226.621 kb on - strand at 1226.621 kb on - strand at 1226.621 kb on - strand at 1226.626 kb on - strand at 1226.629 kb on + strand at 1226.629 kb on + strand at 1226.629 kb on + strand at 1226.629 kb on + strand at 1226.629 kb on + strand at 1226.629 kb on + strand at 1226.630 kb on - strand at 1226.630 kb on - strand at 1226.630 kb on - strand at 1226.630 kb on - strand at 1226.630 kb on - strand at 1226.630 kb on - strand at 1226.630 kb on - strand at 1226.640 kb on - strand at 1226.640 kb on - strand at 1226.640 kb on - strand at 1226.643 kb on + strand at 1226.643 kb on + strand at 1226.643 kb on + strand at 1226.643 kb on + strand at 1226.643 kb on + strand at 1226.644 kb on - strand at 1226.687 kb on + strand at 1226.687 kb on + strand at 1226.687 kb on + strand at 1226.688 kb on - strand at 1226.688 kb on - strand at 1226.706 kb on + strand at 1226.706 kb on + strand at 1226.706 kb on + strand at 1226.706 kb on + strand at 1226.706 kb on + strand at 1226.707 kb on - strand at 1226.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,224,034 + -1.0 1,224,080 - -1.3 1,224,127 + -0.0 1,224,150 + -1.0 1,224,164 - -2.2 1,224,191 + -1.6 1,224,191 + -0.4 1,224,192 - -1.0 1,224,192 - +0.3 1,224,192 - -0.0 1,224,192 - -1.2 1,224,257 - -1.0 1,224,259 + -0.6 1,224,259 + +0.3 1,224,259 + +0.3 1,224,259 + +1.0 1,224,259 + +0.8 1,224,260 - +0.7 1,224,260 - -0.8 1,224,260 - +1.0 1,224,260 - -0.0 1,224,260 - -0.0 1,224,260 - -1.3 1,224,260 - +0.6 1,224,260 - -0.7 1,224,260 - -1.6 1,224,265 + -0.3 1,224,265 + +0.2 1,224,265 + -0.2 1,224,265 + -0.5 1,224,265 + +0.4 1,224,265 + -1.0 1,224,265 + -1.1 1,224,266 - +0.2 1,224,266 - -0.0 1,224,266 - +0.4 1,224,266 - -0.8 1,224,266 - +1.4 1,224,266 - -2.1 1,224,266 - -1.6 1,224,269 + -0.8 1,224,269 + -0.8 1,224,270 - +0.5 1,224,470 + DZA65_RS05935 0.38 -0.6 1,224,471 - DZA65_RS05935 0.39 -2.6 1,224,471 - DZA65_RS05935 0.39 +0.6 1,224,471 - DZA65_RS05935 0.39 +0.5 1,224,487 - DZA65_RS05935 0.41 +0.3 1,224,687 + DZA65_RS05935 0.74 -0.2 1,224,839 + +1.3 1,224,839 + -0.0 1,224,859 - +0.3 1,224,861 + +2.0 1,224,861 + -0.6 1,224,861 + -1.8 1,224,861 + -2.0 1,224,861 + -0.3 1,224,862 - -0.8 1,224,862 - -1.3 1,224,862 - -1.3 1,224,862 - -1.3 1,224,862 - -0.7 1,224,862 - -2.1 1,224,862 - -1.3 1,224,862 - -1.3 1,224,863 + -0.6 1,224,864 - -1.0 1,224,864 - -1.3 1,224,864 - -1.3 1,224,864 - -1.8 1,224,927 - DZA65_RS05940 0.14 +1.0 1,224,936 + DZA65_RS05940 0.15 -1.4 1,224,936 + DZA65_RS05940 0.15 -0.3 1,224,936 + DZA65_RS05940 0.15 -0.8 1,224,936 + DZA65_RS05940 0.15 -0.3 1,224,936 + DZA65_RS05940 0.15 -0.8 1,224,936 + DZA65_RS05940 0.15 -0.6 1,224,937 - DZA65_RS05940 0.15 -1.3 1,224,937 - DZA65_RS05940 0.15 -2.2 1,224,937 - DZA65_RS05940 0.15 -0.9 1,224,937 - DZA65_RS05940 0.15 -0.5 1,224,937 - DZA65_RS05940 0.15 -2.0 1,224,938 + DZA65_RS05940 0.15 -0.8 1,224,938 + DZA65_RS05940 0.15 -1.8 1,224,938 + DZA65_RS05940 0.15 -0.4 1,224,938 + DZA65_RS05940 0.15 -0.6 1,224,938 + DZA65_RS05940 0.15 -0.2 1,224,938 + DZA65_RS05940 0.15 -0.0 1,224,938 + DZA65_RS05940 0.15 -1.3 1,224,938 + DZA65_RS05940 0.15 -2.4 1,224,938 + DZA65_RS05940 0.15 +1.0 1,224,939 - DZA65_RS05940 0.15 -1.0 1,224,939 - DZA65_RS05940 0.15 -3.6 1,224,939 - DZA65_RS05940 0.15 -0.3 1,224,939 - DZA65_RS05940 0.15 -0.8 1,224,991 - DZA65_RS05940 0.21 -2.0 1,225,025 + DZA65_RS05940 0.24 -1.9 1,225,025 + DZA65_RS05940 0.24 -1.2 1,225,025 + DZA65_RS05940 0.24 +0.4 1,225,025 + DZA65_RS05940 0.24 -1.4 1,225,025 + DZA65_RS05940 0.24 -2.0 1,225,025 + DZA65_RS05940 0.24 -1.3 1,225,025 + DZA65_RS05940 0.24 -0.0 1,225,025 + DZA65_RS05940 0.24 -0.2 1,225,026 - DZA65_RS05940 0.24 -1.3 1,225,026 - DZA65_RS05940 0.24 -0.3 1,225,026 - DZA65_RS05940 0.24 -0.2 1,225,026 - DZA65_RS05940 0.24 -0.6 1,225,026 - DZA65_RS05940 0.24 -2.5 1,225,026 - DZA65_RS05940 0.24 -0.9 1,225,026 - DZA65_RS05940 0.24 -1.6 1,225,026 - DZA65_RS05940 0.24 +0.4 1,225,026 - DZA65_RS05940 0.24 -1.9 1,225,026 - DZA65_RS05940 0.24 +0.2 1,225,050 + DZA65_RS05940 0.27 -2.3 1,225,164 + DZA65_RS05940 0.39 -1.2 1,225,164 + DZA65_RS05940 0.39 -1.8 1,225,216 - DZA65_RS05940 0.45 -0.5 1,225,302 + DZA65_RS05940 0.54 -0.9 1,225,303 - DZA65_RS05940 0.54 -1.5 1,225,441 + DZA65_RS05940 0.69 -0.6 1,225,441 + DZA65_RS05940 0.69 -2.0 1,225,441 + DZA65_RS05940 0.69 -2.6 1,225,441 + DZA65_RS05940 0.69 -1.3 1,225,442 - DZA65_RS05940 0.69 -0.2 1,225,488 + DZA65_RS05940 0.74 -1.8 1,225,495 + DZA65_RS05940 0.75 -2.7 1,225,496 - DZA65_RS05940 0.75 +0.5 1,225,569 + DZA65_RS05940 0.83 -2.3 1,225,569 + DZA65_RS05940 0.83 -1.0 1,225,569 + DZA65_RS05940 0.83 -2.4 1,225,569 + DZA65_RS05940 0.83 -2.0 1,225,575 + DZA65_RS05940 0.84 -0.0 1,225,575 + DZA65_RS05940 0.84 +0.4 1,225,575 + DZA65_RS05940 0.84 -1.0 1,225,575 + DZA65_RS05940 0.84 -3.0 1,225,576 - DZA65_RS05940 0.84 -1.0 1,225,576 - DZA65_RS05940 0.84 +1.0 1,225,576 - DZA65_RS05940 0.84 -1.0 1,225,599 + DZA65_RS05940 0.87 -2.0 1,225,600 - DZA65_RS05940 0.87 -0.8 1,225,630 - DZA65_RS05940 0.90 -2.6 1,225,645 + +0.1 1,225,645 + -1.0 1,225,645 + -0.6 1,225,645 + -1.2 1,225,645 + -2.2 1,225,645 + -1.0 1,225,645 + -1.3 1,225,645 + -1.3 1,225,645 + -0.4 1,225,645 + -2.2 1,225,645 + -1.4 1,225,645 + -2.6 1,225,646 - -0.1 1,225,666 + -1.8 1,225,667 - -0.3 1,225,667 - -2.2 1,225,769 + -2.0 1,225,769 + +0.6 1,225,769 + +0.3 1,225,770 - +0.7 1,225,770 - -0.0 1,225,770 - -0.0 1,225,774 + -2.5 1,225,775 - +1.0 1,225,779 + +0.1 1,225,779 + +0.2 1,225,779 + -1.0 1,225,779 + -0.6 1,225,779 + +0.5 1,225,842 + -1.0 1,225,842 + +0.0 1,225,843 - -0.2 1,225,843 - +0.1 1,225,910 - +0.7 1,225,943 - +0.7 1,225,993 + DZA65_RS05945 0.20 -0.9 1,225,994 - DZA65_RS05945 0.20 -0.2 1,226,035 + DZA65_RS05945 0.28 +1.4 1,226,036 - DZA65_RS05945 0.29 +0.3 1,226,036 - DZA65_RS05945 0.29 +0.6 1,226,168 + DZA65_RS05945 0.55 +0.7 1,226,169 - DZA65_RS05945 0.56 +0.1 1,226,322 + DZA65_RS05945 0.87 +0.5 1,226,322 + DZA65_RS05945 0.87 +0.0 1,226,323 - DZA65_RS05945 0.87 -0.3 1,226,371 + +0.4 1,226,394 + +1.0 1,226,394 + -0.0 1,226,436 + -1.4 1,226,466 + +1.6 1,226,467 - -0.3 1,226,467 - -0.0 1,226,539 - -0.6 1,226,620 + -0.1 1,226,620 + +1.2 1,226,620 + -0.2 1,226,621 - +0.2 1,226,621 - -1.3 1,226,621 - +0.1 1,226,621 - -1.3 1,226,626 - -1.4 1,226,629 + -0.6 1,226,629 + -2.2 1,226,629 + +0.3 1,226,629 + +0.4 1,226,629 + -0.2 1,226,629 + +0.7 1,226,630 - +1.0 1,226,630 - -0.4 1,226,630 - +0.1 1,226,630 - -0.8 1,226,630 - -0.0 1,226,630 - -0.2 1,226,630 - -1.6 1,226,640 - -0.0 1,226,640 - -0.5 1,226,640 - -0.3 1,226,643 + -1.2 1,226,643 + +0.5 1,226,643 + -0.1 1,226,643 + +2.1 1,226,643 + -0.4 1,226,644 - +0.6 1,226,687 + +1.0 1,226,687 + +0.1 1,226,687 + -0.8 1,226,688 - -0.3 1,226,688 - -0.0 1,226,706 + -1.3 1,226,706 + -0.3 1,226,706 + -1.0 1,226,706 + -1.8 1,226,706 + +0.7 1,226,707 - -0.8 1,226,707 - -1.1
Or see this region's nucleotide sequence