Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS03315 and DZA65_RS03320 are separated by 99 nucleotides DZA65_RS03320 and DZA65_RS03325 are separated by 122 nucleotides DZA65_RS03325 and DZA65_RS03330 are separated by 1 nucleotides DZA65_RS03330 and DZA65_RS03335 overlap by 1 nucleotides
DZA65_RS03315: DZA65_RS03315 - phosphopentomutase, at 702,220 to 703,443
_RS03315
DZA65_RS03320: DZA65_RS03320 - purine-nucleoside phosphorylase, at 703,543 to 704,268
_RS03320
DZA65_RS03325: DZA65_RS03325 - ATP-grasp domain-containing protein, at 704,391 to 705,629
_RS03325
DZA65_RS03330: DZA65_RS03330 - EamA family transporter, at 705,631 to 706,563
_RS03330
DZA65_RS03335: DZA65_RS03335 - YggS family pyridoxal phosphate-dependent enzyme, at 706,563 to 707,222
_RS03335
Position (kb)
704
705
706 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 703.465 kb on - strand at 703.492 kb on - strand at 703.493 kb on + strand at 703.493 kb on + strand at 703.493 kb on + strand at 703.495 kb on + strand at 703.495 kb on + strand at 703.495 kb on + strand at 703.495 kb on + strand at 703.495 kb on + strand at 703.495 kb on + strand at 703.495 kb on + strand at 703.495 kb on + strand at 703.496 kb on - strand at 703.496 kb on - strand at 703.496 kb on - strand at 703.548 kb on + strand at 703.549 kb on - strand at 703.557 kb on + strand at 703.557 kb on + strand at 703.557 kb on + strand at 703.557 kb on + strand at 703.557 kb on + strand at 703.557 kb on + strand at 703.557 kb on + strand at 703.557 kb on + strand at 703.558 kb on - strand at 703.558 kb on - strand at 703.624 kb on + strand, within DZA65_RS03320 at 703.625 kb on - strand, within DZA65_RS03320 at 703.625 kb on - strand, within DZA65_RS03320 at 703.625 kb on - strand, within DZA65_RS03320 at 703.700 kb on - strand, within DZA65_RS03320 at 703.759 kb on + strand, within DZA65_RS03320 at 703.759 kb on + strand, within DZA65_RS03320 at 704.023 kb on + strand, within DZA65_RS03320 at 704.024 kb on - strand, within DZA65_RS03320 at 704.024 kb on - strand, within DZA65_RS03320 at 704.062 kb on + strand, within DZA65_RS03320 at 704.101 kb on + strand, within DZA65_RS03320 at 704.102 kb on - strand, within DZA65_RS03320 at 704.128 kb on - strand, within DZA65_RS03320 at 704.309 kb on + strand at 704.309 kb on + strand at 704.310 kb on - strand at 704.364 kb on + strand at 704.373 kb on + strand at 704.374 kb on - strand at 704.377 kb on + strand at 704.397 kb on + strand at 704.398 kb on - strand at 704.437 kb on + strand at 704.437 kb on + strand at 704.438 kb on - strand at 704.438 kb on - strand at 704.438 kb on - strand at 704.438 kb on - strand at 704.438 kb on - strand at 704.476 kb on + strand at 704.476 kb on + strand at 704.477 kb on - strand at 704.480 kb on + strand at 704.480 kb on + strand at 704.481 kb on - strand at 704.481 kb on - strand at 704.483 kb on + strand at 704.483 kb on + strand at 704.484 kb on - strand at 704.539 kb on + strand, within DZA65_RS03325 at 704.540 kb on - strand, within DZA65_RS03325 at 704.658 kb on - strand, within DZA65_RS03325 at 704.713 kb on - strand, within DZA65_RS03325 at 704.769 kb on + strand, within DZA65_RS03325 at 704.817 kb on - strand, within DZA65_RS03325 at 704.882 kb on + strand, within DZA65_RS03325 at 704.883 kb on - strand, within DZA65_RS03325 at 704.884 kb on + strand, within DZA65_RS03325 at 704.885 kb on - strand, within DZA65_RS03325 at 704.949 kb on - strand, within DZA65_RS03325 at 705.064 kb on - strand, within DZA65_RS03325 at 705.226 kb on - strand, within DZA65_RS03325 at 705.374 kb on + strand, within DZA65_RS03325 at 705.452 kb on + strand, within DZA65_RS03325 at 705.493 kb on + strand, within DZA65_RS03325 at 705.511 kb on + strand at 705.512 kb on - strand at 705.542 kb on + strand at 705.542 kb on + strand at 705.543 kb on - strand at 705.543 kb on - strand at 705.549 kb on + strand at 705.578 kb on + strand at 705.584 kb on + strand at 705.785 kb on - strand, within DZA65_RS03330 at 705.799 kb on + strand, within DZA65_RS03330 at 705.799 kb on + strand, within DZA65_RS03330 at 705.799 kb on + strand, within DZA65_RS03330 at 705.799 kb on + strand, within DZA65_RS03330 at 705.800 kb on - strand, within DZA65_RS03330 at 705.841 kb on - strand, within DZA65_RS03330 at 705.844 kb on + strand, within DZA65_RS03330 at 705.845 kb on - strand, within DZA65_RS03330 at 705.847 kb on - strand, within DZA65_RS03330 at 705.847 kb on - strand, within DZA65_RS03330 at 705.913 kb on - strand, within DZA65_RS03330 at 705.918 kb on + strand, within DZA65_RS03330 at 705.919 kb on - strand, within DZA65_RS03330 at 705.919 kb on - strand, within DZA65_RS03330 at 705.940 kb on + strand, within DZA65_RS03330 at 705.956 kb on - strand, within DZA65_RS03330 at 706.039 kb on + strand, within DZA65_RS03330 at 706.097 kb on - strand, within DZA65_RS03330 at 706.138 kb on + strand, within DZA65_RS03330 at 706.219 kb on + strand, within DZA65_RS03330 at 706.221 kb on + strand, within DZA65_RS03330 at 706.222 kb on - strand, within DZA65_RS03330 at 706.222 kb on - strand, within DZA65_RS03330 at 706.226 kb on - strand, within DZA65_RS03330 at 706.226 kb on - strand, within DZA65_RS03330 at 706.226 kb on - strand, within DZA65_RS03330 at 706.231 kb on + strand, within DZA65_RS03330 at 706.279 kb on + strand, within DZA65_RS03330 at 706.298 kb on - strand, within DZA65_RS03330 at 706.345 kb on + strand, within DZA65_RS03330 at 706.346 kb on - strand, within DZA65_RS03330 at 706.392 kb on + strand, within DZA65_RS03330 at 706.415 kb on - strand, within DZA65_RS03330 at 706.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 703,465 - +1.2 703,492 - -2.7 703,493 + -0.8 703,493 + +0.6 703,493 + -0.4 703,495 + +0.2 703,495 + +0.6 703,495 + -1.5 703,495 + +0.3 703,495 + +0.4 703,495 + +0.0 703,495 + -0.8 703,495 + -2.3 703,496 - -0.4 703,496 - +0.3 703,496 - -0.1 703,548 + -0.6 703,549 - -0.3 703,557 + -0.3 703,557 + -0.3 703,557 + +1.6 703,557 + -1.4 703,557 + +0.2 703,557 + -1.2 703,557 + -0.3 703,557 + +1.6 703,558 - -0.6 703,558 - -1.0 703,624 + DZA65_RS03320 0.11 +1.2 703,625 - DZA65_RS03320 0.11 +1.6 703,625 - DZA65_RS03320 0.11 +1.0 703,625 - DZA65_RS03320 0.11 -2.3 703,700 - DZA65_RS03320 0.22 +0.6 703,759 + DZA65_RS03320 0.30 +0.8 703,759 + DZA65_RS03320 0.30 +0.6 704,023 + DZA65_RS03320 0.66 -0.4 704,024 - DZA65_RS03320 0.66 -0.4 704,024 - DZA65_RS03320 0.66 +2.2 704,062 + DZA65_RS03320 0.71 -0.3 704,101 + DZA65_RS03320 0.77 -0.2 704,102 - DZA65_RS03320 0.77 -0.7 704,128 - DZA65_RS03320 0.81 -1.0 704,309 + -1.0 704,309 + -0.3 704,310 - +0.0 704,364 + -0.4 704,373 + +0.2 704,374 - +0.4 704,377 + +1.4 704,397 + -0.7 704,398 - -1.7 704,437 + -0.1 704,437 + +1.0 704,438 - +0.6 704,438 - +0.8 704,438 - -0.3 704,438 - -1.3 704,438 - -0.7 704,476 + -0.6 704,476 + -2.2 704,477 - -0.6 704,480 + +0.5 704,480 + +0.2 704,481 - -0.5 704,481 - +0.1 704,483 + +0.0 704,483 + +0.7 704,484 - -0.8 704,539 + DZA65_RS03325 0.12 +0.4 704,540 - DZA65_RS03325 0.12 +0.7 704,658 - DZA65_RS03325 0.22 -1.0 704,713 - DZA65_RS03325 0.26 -0.4 704,769 + DZA65_RS03325 0.31 -0.4 704,817 - DZA65_RS03325 0.34 +0.5 704,882 + DZA65_RS03325 0.40 -1.7 704,883 - DZA65_RS03325 0.40 +0.1 704,884 + DZA65_RS03325 0.40 +0.6 704,885 - DZA65_RS03325 0.40 +0.2 704,949 - DZA65_RS03325 0.45 +0.2 705,064 - DZA65_RS03325 0.54 -1.7 705,226 - DZA65_RS03325 0.67 +0.6 705,374 + DZA65_RS03325 0.79 +0.0 705,452 + DZA65_RS03325 0.86 -0.4 705,493 + DZA65_RS03325 0.89 +1.4 705,511 + -0.2 705,512 - -1.4 705,542 + -2.7 705,542 + -0.7 705,543 - +0.2 705,543 - -0.1 705,549 + +0.3 705,578 + +0.2 705,584 + -0.1 705,785 - DZA65_RS03330 0.17 +1.1 705,799 + DZA65_RS03330 0.18 -0.8 705,799 + DZA65_RS03330 0.18 +1.7 705,799 + DZA65_RS03330 0.18 -0.6 705,799 + DZA65_RS03330 0.18 +0.6 705,800 - DZA65_RS03330 0.18 -2.3 705,841 - DZA65_RS03330 0.23 +1.2 705,844 + DZA65_RS03330 0.23 +0.9 705,845 - DZA65_RS03330 0.23 -0.2 705,847 - DZA65_RS03330 0.23 +1.8 705,847 - DZA65_RS03330 0.23 -0.0 705,913 - DZA65_RS03330 0.30 +0.9 705,918 + DZA65_RS03330 0.31 +0.2 705,919 - DZA65_RS03330 0.31 +0.1 705,919 - DZA65_RS03330 0.31 +0.7 705,940 + DZA65_RS03330 0.33 -0.2 705,956 - DZA65_RS03330 0.35 +0.7 706,039 + DZA65_RS03330 0.44 -0.7 706,097 - DZA65_RS03330 0.50 -0.3 706,138 + DZA65_RS03330 0.54 +0.8 706,219 + DZA65_RS03330 0.63 +1.2 706,221 + DZA65_RS03330 0.63 +0.3 706,222 - DZA65_RS03330 0.63 +0.9 706,222 - DZA65_RS03330 0.63 +0.4 706,226 - DZA65_RS03330 0.64 +0.2 706,226 - DZA65_RS03330 0.64 +1.5 706,226 - DZA65_RS03330 0.64 -2.2 706,231 + DZA65_RS03330 0.64 -1.2 706,279 + DZA65_RS03330 0.69 +0.9 706,298 - DZA65_RS03330 0.71 -0.8 706,345 + DZA65_RS03330 0.77 +1.6 706,346 - DZA65_RS03330 0.77 -0.1 706,392 + DZA65_RS03330 0.82 -0.6 706,415 - DZA65_RS03330 0.84 +0.7 706,507 - -1.3
Or see this region's nucleotide sequence