Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01490 and DZA65_RS01495 overlap by 4 nucleotides DZA65_RS01495 and DZA65_RS01500 are separated by 145 nucleotides DZA65_RS01500 and DZA65_RS01505 are separated by 92 nucleotides DZA65_RS01505 and DZA65_RS01510 are separated by 24 nucleotides
DZA65_RS01490: DZA65_RS01490 - PTS phosphocarrier protein NPr, at 329,311 to 329,583
_RS01490
DZA65_RS01495: DZA65_RS01495 - RNase adapter RapZ, at 329,580 to 330,434
_RS01495
DZA65_RS01500: DZA65_RS01500 - PTS IIA-like nitrogen regulatory protein PtsN, at 330,580 to 331,068
_RS01500
DZA65_RS01505: DZA65_RS01505 - ribosome hibernation promoting factor, at 331,161 to 331,448
_RS01505
DZA65_RS01510: DZA65_RS01510 - RNA polymerase factor sigma-54, at 331,473 to 332,906
_RS01510
Position (kb) 330
331
332 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 329.604 kb on - strand at 329.645 kb on - strand at 329.645 kb on - strand at 329.666 kb on + strand, within DZA65_RS01495 at 329.668 kb on + strand, within DZA65_RS01495 at 329.668 kb on + strand, within DZA65_RS01495 at 329.668 kb on + strand, within DZA65_RS01495 at 329.668 kb on + strand, within DZA65_RS01495 at 329.669 kb on - strand, within DZA65_RS01495 at 329.669 kb on - strand at 329.669 kb on - strand, within DZA65_RS01495 at 329.712 kb on - strand, within DZA65_RS01495 at 329.712 kb on - strand, within DZA65_RS01495 at 329.716 kb on + strand, within DZA65_RS01495 at 329.716 kb on + strand at 329.717 kb on - strand, within DZA65_RS01495 at 329.717 kb on - strand, within DZA65_RS01495 at 329.717 kb on - strand at 329.761 kb on + strand, within DZA65_RS01495 at 329.762 kb on - strand, within DZA65_RS01495 at 329.776 kb on + strand, within DZA65_RS01495 at 329.776 kb on + strand, within DZA65_RS01495 at 329.776 kb on + strand, within DZA65_RS01495 at 329.776 kb on + strand, within DZA65_RS01495 at 329.776 kb on + strand, within DZA65_RS01495 at 329.777 kb on - strand, within DZA65_RS01495 at 329.777 kb on - strand, within DZA65_RS01495 at 329.777 kb on - strand, within DZA65_RS01495 at 329.900 kb on - strand, within DZA65_RS01495 at 329.900 kb on - strand, within DZA65_RS01495 at 329.900 kb on - strand, within DZA65_RS01495 at 329.900 kb on - strand, within DZA65_RS01495 at 329.900 kb on - strand, within DZA65_RS01495 at 329.900 kb on - strand, within DZA65_RS01495 at 330.029 kb on + strand, within DZA65_RS01495 at 330.030 kb on - strand, within DZA65_RS01495 at 330.030 kb on - strand at 330.089 kb on + strand, within DZA65_RS01495 at 330.164 kb on + strand, within DZA65_RS01495 at 330.164 kb on + strand at 330.165 kb on - strand, within DZA65_RS01495 at 330.291 kb on - strand, within DZA65_RS01495 at 330.356 kb on - strand at 330.356 kb on - strand at 330.482 kb on + strand at 330.483 kb on - strand at 330.483 kb on - strand at 330.483 kb on - strand at 330.483 kb on - strand at 330.486 kb on + strand at 330.487 kb on - strand at 330.487 kb on - strand at 330.487 kb on - strand at 330.539 kb on + strand at 330.539 kb on + strand at 330.539 kb on + strand at 330.539 kb on + strand at 330.540 kb on - strand at 330.540 kb on - strand at 330.540 kb on - strand at 330.540 kb on - strand at 330.540 kb on - strand at 330.540 kb on - strand at 330.540 kb on - strand at 330.702 kb on + strand, within DZA65_RS01500 at 330.702 kb on + strand, within DZA65_RS01500 at 330.702 kb on + strand, within DZA65_RS01500 at 330.703 kb on - strand, within DZA65_RS01500 at 330.703 kb on - strand, within DZA65_RS01500 at 330.703 kb on - strand, within DZA65_RS01500 at 330.703 kb on - strand, within DZA65_RS01500 at 330.706 kb on + strand, within DZA65_RS01500 at 330.707 kb on - strand, within DZA65_RS01500 at 330.707 kb on - strand, within DZA65_RS01500 at 330.707 kb on - strand, within DZA65_RS01500 at 330.707 kb on - strand, within DZA65_RS01500 at 330.707 kb on - strand, within DZA65_RS01500 at 330.707 kb on - strand, within DZA65_RS01500 at 330.707 kb on - strand, within DZA65_RS01500 at 330.717 kb on + strand, within DZA65_RS01500 at 330.717 kb on + strand, within DZA65_RS01500 at 330.718 kb on - strand, within DZA65_RS01500 at 330.831 kb on + strand, within DZA65_RS01500 at 330.832 kb on - strand, within DZA65_RS01500 at 330.832 kb on - strand, within DZA65_RS01500 at 330.895 kb on - strand, within DZA65_RS01500 at 330.895 kb on - strand, within DZA65_RS01500 at 330.895 kb on - strand, within DZA65_RS01500 at 331.011 kb on + strand, within DZA65_RS01500 at 331.017 kb on + strand, within DZA65_RS01500 at 331.017 kb on + strand, within DZA65_RS01500 at 331.018 kb on - strand, within DZA65_RS01500 at 331.018 kb on - strand, within DZA65_RS01500 at 331.042 kb on - strand at 331.071 kb on + strand at 331.071 kb on + strand at 331.071 kb on + strand at 331.071 kb on + strand at 331.071 kb on + strand at 331.071 kb on + strand at 331.071 kb on + strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.072 kb on - strand at 331.081 kb on + strand at 331.081 kb on + strand at 331.082 kb on - strand at 331.082 kb on - strand at 331.082 kb on - strand at 331.119 kb on + strand at 331.120 kb on - strand at 331.120 kb on - strand at 331.120 kb on - strand at 331.184 kb on + strand at 331.184 kb on + strand at 331.185 kb on - strand at 331.185 kb on - strand at 331.237 kb on + strand, within DZA65_RS01505 at 331.237 kb on + strand, within DZA65_RS01505 at 331.237 kb on + strand, within DZA65_RS01505 at 331.237 kb on + strand, within DZA65_RS01505 at 331.237 kb on + strand, within DZA65_RS01505 at 331.238 kb on - strand, within DZA65_RS01505 at 331.238 kb on - strand, within DZA65_RS01505 at 331.238 kb on - strand, within DZA65_RS01505 at 331.238 kb on - strand, within DZA65_RS01505 at 331.238 kb on - strand, within DZA65_RS01505 at 331.238 kb on - strand, within DZA65_RS01505 at 331.241 kb on + strand, within DZA65_RS01505 at 331.242 kb on - strand, within DZA65_RS01505 at 331.242 kb on - strand, within DZA65_RS01505 at 331.242 kb on - strand, within DZA65_RS01505 at 331.336 kb on + strand, within DZA65_RS01505 at 331.336 kb on + strand, within DZA65_RS01505 at 331.337 kb on - strand, within DZA65_RS01505 at 331.337 kb on - strand, within DZA65_RS01505 at 331.349 kb on + strand, within DZA65_RS01505 at 331.350 kb on - strand, within DZA65_RS01505 at 331.350 kb on - strand, within DZA65_RS01505 at 331.360 kb on + strand, within DZA65_RS01505 at 331.361 kb on - strand, within DZA65_RS01505 at 331.439 kb on + strand at 331.439 kb on + strand at 331.440 kb on - strand at 331.448 kb on + strand at 331.467 kb on + strand at 331.468 kb on - strand at 331.488 kb on - strand at 331.488 kb on - strand at 331.512 kb on + strand at 331.512 kb on + strand at 331.513 kb on - strand at 331.513 kb on - strand at 331.513 kb on - strand at 331.513 kb on - strand at 331.706 kb on - strand, within DZA65_RS01510 at 331.706 kb on - strand, within DZA65_RS01510 at 331.706 kb on - strand, within DZA65_RS01510 at 331.722 kb on - strand, within DZA65_RS01510 at 331.733 kb on + strand, within DZA65_RS01510 at 331.814 kb on + strand, within DZA65_RS01510 at 331.815 kb on - strand, within DZA65_RS01510 at 331.815 kb on - strand, within DZA65_RS01510 at 332.000 kb on - strand, within DZA65_RS01510
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 329,604 - +1.3 329,645 - +1.7 329,645 - +1.3 329,666 + DZA65_RS01495 0.10 -0.4 329,668 + DZA65_RS01495 0.10 -1.3 329,668 + DZA65_RS01495 0.10 +0.4 329,668 + DZA65_RS01495 0.10 +1.3 329,668 + DZA65_RS01495 0.10 -0.5 329,669 - DZA65_RS01495 0.10 +0.9 329,669 - +4.1 329,669 - DZA65_RS01495 0.10 +0.9 329,712 - DZA65_RS01495 0.15 +1.0 329,712 - DZA65_RS01495 0.15 +0.7 329,716 + DZA65_RS01495 0.16 -0.4 329,716 + +0.9 329,717 - DZA65_RS01495 0.16 -1.5 329,717 - DZA65_RS01495 0.16 +0.6 329,717 - +1.3 329,761 + DZA65_RS01495 0.21 +1.6 329,762 - DZA65_RS01495 0.21 +0.1 329,776 + DZA65_RS01495 0.23 +0.9 329,776 + DZA65_RS01495 0.23 -0.0 329,776 + DZA65_RS01495 0.23 +0.3 329,776 + DZA65_RS01495 0.23 -0.7 329,776 + DZA65_RS01495 0.23 +0.2 329,777 - DZA65_RS01495 0.23 +0.7 329,777 - DZA65_RS01495 0.23 +0.5 329,777 - DZA65_RS01495 0.23 +0.0 329,900 - DZA65_RS01495 0.37 +0.9 329,900 - DZA65_RS01495 0.37 -0.9 329,900 - DZA65_RS01495 0.37 +0.4 329,900 - DZA65_RS01495 0.37 +0.7 329,900 - DZA65_RS01495 0.37 +0.8 329,900 - DZA65_RS01495 0.37 +1.2 330,029 + DZA65_RS01495 0.53 -1.7 330,030 - DZA65_RS01495 0.53 +1.3 330,030 - -1.3 330,089 + DZA65_RS01495 0.60 -0.0 330,164 + DZA65_RS01495 0.68 +0.6 330,164 + +0.7 330,165 - DZA65_RS01495 0.68 +0.4 330,291 - DZA65_RS01495 0.83 +0.7 330,356 - -1.9 330,356 - -0.5 330,482 + +0.3 330,483 - +0.1 330,483 - -1.5 330,483 - +0.0 330,483 - -1.1 330,486 + -1.8 330,487 - -1.3 330,487 - -0.4 330,487 - +0.1 330,539 + -0.8 330,539 + +0.2 330,539 + -1.1 330,539 + +0.8 330,540 - -0.3 330,540 - -1.8 330,540 - -0.3 330,540 - +0.6 330,540 - -0.3 330,540 - +0.5 330,540 - -1.5 330,702 + DZA65_RS01500 0.25 +1.3 330,702 + DZA65_RS01500 0.25 -0.3 330,702 + DZA65_RS01500 0.25 -0.6 330,703 - DZA65_RS01500 0.25 -1.3 330,703 - DZA65_RS01500 0.25 -0.3 330,703 - DZA65_RS01500 0.25 -1.9 330,703 - DZA65_RS01500 0.25 +1.3 330,706 + DZA65_RS01500 0.26 -1.3 330,707 - DZA65_RS01500 0.26 -2.6 330,707 - DZA65_RS01500 0.26 -1.9 330,707 - DZA65_RS01500 0.26 -2.4 330,707 - DZA65_RS01500 0.26 -2.4 330,707 - DZA65_RS01500 0.26 -1.4 330,707 - DZA65_RS01500 0.26 -1.5 330,707 - DZA65_RS01500 0.26 +1.4 330,717 + DZA65_RS01500 0.28 -0.7 330,717 + DZA65_RS01500 0.28 -0.0 330,718 - DZA65_RS01500 0.28 -1.3 330,831 + DZA65_RS01500 0.51 -0.7 330,832 - DZA65_RS01500 0.52 -1.7 330,832 - DZA65_RS01500 0.52 -0.5 330,895 - DZA65_RS01500 0.64 -0.3 330,895 - DZA65_RS01500 0.64 -2.7 330,895 - DZA65_RS01500 0.64 -1.7 331,011 + DZA65_RS01500 0.88 -1.5 331,017 + DZA65_RS01500 0.89 -2.5 331,017 + DZA65_RS01500 0.89 -2.3 331,018 - DZA65_RS01500 0.90 -1.4 331,018 - DZA65_RS01500 0.90 +0.6 331,042 - -1.8 331,071 + +0.6 331,071 + -1.7 331,071 + -1.7 331,071 + -2.0 331,071 + -1.3 331,071 + +0.7 331,071 + -1.6 331,072 - +0.7 331,072 - +3.2 331,072 - +0.3 331,072 - -0.3 331,072 - -1.6 331,072 - +0.2 331,072 - -0.0 331,072 - -0.6 331,072 - +1.6 331,072 - -0.4 331,072 - +0.5 331,072 - -0.3 331,072 - -0.3 331,072 - +1.1 331,072 - +0.2 331,081 + +1.3 331,081 + -1.9 331,082 - +1.0 331,082 - -0.5 331,082 - -0.8 331,119 + -2.8 331,120 - -0.7 331,120 - -0.8 331,120 - -0.3 331,184 + -0.0 331,184 + +0.5 331,185 - +0.7 331,185 - -1.0 331,237 + DZA65_RS01505 0.26 -0.7 331,237 + DZA65_RS01505 0.26 +1.3 331,237 + DZA65_RS01505 0.26 -0.3 331,237 + DZA65_RS01505 0.26 +1.1 331,237 + DZA65_RS01505 0.26 -0.0 331,238 - DZA65_RS01505 0.27 +0.9 331,238 - DZA65_RS01505 0.27 -1.3 331,238 - DZA65_RS01505 0.27 +0.2 331,238 - DZA65_RS01505 0.27 -1.3 331,238 - DZA65_RS01505 0.27 -1.3 331,238 - DZA65_RS01505 0.27 -0.5 331,241 + DZA65_RS01505 0.28 -0.6 331,242 - DZA65_RS01505 0.28 +0.2 331,242 - DZA65_RS01505 0.28 +1.6 331,242 - DZA65_RS01505 0.28 -1.3 331,336 + DZA65_RS01505 0.61 +0.6 331,336 + DZA65_RS01505 0.61 +1.3 331,337 - DZA65_RS01505 0.61 +0.7 331,337 - DZA65_RS01505 0.61 +0.3 331,349 + DZA65_RS01505 0.65 -2.2 331,350 - DZA65_RS01505 0.66 +0.3 331,350 - DZA65_RS01505 0.66 +0.4 331,360 + DZA65_RS01505 0.69 -1.0 331,361 - DZA65_RS01505 0.69 -0.1 331,439 + -0.7 331,439 + +0.3 331,440 - +0.6 331,448 + +0.2 331,467 + -2.2 331,468 - -0.5 331,488 - -0.3 331,488 - +1.0 331,512 + -4.6 331,512 + -2.7 331,513 - +0.7 331,513 - -1.3 331,513 - -3.0 331,513 - -1.3 331,706 - DZA65_RS01510 0.16 +0.3 331,706 - DZA65_RS01510 0.16 -2.3 331,706 - DZA65_RS01510 0.16 -1.0 331,722 - DZA65_RS01510 0.17 -1.9 331,733 + DZA65_RS01510 0.18 -2.7 331,814 + DZA65_RS01510 0.24 -1.5 331,815 - DZA65_RS01510 0.24 -2.3 331,815 - DZA65_RS01510 0.24 -2.2 332,000 - DZA65_RS01510 0.37 -1.0
Or see this region's nucleotide sequence