Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS00730 and DZA65_RS00735 are separated by 112 nucleotides DZA65_RS00735 and DZA65_RS00740 are separated by 48 nucleotides DZA65_RS00740 and DZA65_RS00745 are separated by 108 nucleotides
DZA65_RS00730: DZA65_RS00730 - ribonuclease PH, at 167,772 to 168,488
_RS00730
DZA65_RS00735: DZA65_RS00735 - orotate phosphoribosyltransferase, at 168,601 to 169,242
_RS00735
DZA65_RS00740: DZA65_RS00740 - nucleoid occlusion factor SlmA, at 169,291 to 169,887
_RS00740
DZA65_RS00745: DZA65_RS00745 - dUTP diphosphatase, at 169,996 to 170,454
_RS00745
Position (kb) 168
169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 167.626 kb on + strand at 167.626 kb on + strand at 167.626 kb on + strand at 167.626 kb on + strand at 167.626 kb on + strand at 167.626 kb on + strand at 167.627 kb on - strand at 167.627 kb on - strand at 167.627 kb on - strand at 167.627 kb on - strand at 167.627 kb on - strand at 167.627 kb on - strand at 167.627 kb on - strand at 167.627 kb on - strand at 167.627 kb on - strand at 167.627 kb on - strand at 167.691 kb on + strand at 167.730 kb on + strand at 167.730 kb on + strand at 167.730 kb on + strand at 167.730 kb on + strand at 167.730 kb on + strand at 167.730 kb on + strand at 167.730 kb on + strand at 167.730 kb on + strand at 167.731 kb on - strand at 167.731 kb on - strand at 167.731 kb on - strand at 167.731 kb on - strand at 167.731 kb on - strand at 167.731 kb on - strand at 167.731 kb on - strand at 167.731 kb on - strand at 167.732 kb on + strand at 167.732 kb on + strand at 167.732 kb on + strand at 167.732 kb on + strand at 167.732 kb on + strand at 167.732 kb on + strand at 167.732 kb on + strand at 167.732 kb on + strand at 167.732 kb on + strand at 167.733 kb on - strand at 167.733 kb on - strand at 167.747 kb on + strand at 167.748 kb on - strand at 167.873 kb on + strand, within DZA65_RS00730 at 167.958 kb on + strand, within DZA65_RS00730 at 167.958 kb on + strand, within DZA65_RS00730 at 167.959 kb on - strand at 167.963 kb on + strand, within DZA65_RS00730 at 167.963 kb on + strand, within DZA65_RS00730 at 167.963 kb on + strand, within DZA65_RS00730 at 167.963 kb on + strand, within DZA65_RS00730 at 167.964 kb on - strand, within DZA65_RS00730 at 168.099 kb on + strand at 168.099 kb on + strand, within DZA65_RS00730 at 168.099 kb on + strand, within DZA65_RS00730 at 168.099 kb on + strand, within DZA65_RS00730 at 168.099 kb on + strand, within DZA65_RS00730 at 168.099 kb on + strand, within DZA65_RS00730 at 168.099 kb on + strand, within DZA65_RS00730 at 168.099 kb on + strand, within DZA65_RS00730 at 168.099 kb on + strand, within DZA65_RS00730 at 168.099 kb on + strand, within DZA65_RS00730 at 168.099 kb on + strand, within DZA65_RS00730 at 168.100 kb on - strand, within DZA65_RS00730 at 168.100 kb on - strand, within DZA65_RS00730 at 168.100 kb on - strand, within DZA65_RS00730 at 168.100 kb on - strand, within DZA65_RS00730 at 168.100 kb on - strand, within DZA65_RS00730 at 168.100 kb on - strand, within DZA65_RS00730 at 168.107 kb on + strand, within DZA65_RS00730 at 168.108 kb on - strand, within DZA65_RS00730 at 168.108 kb on - strand, within DZA65_RS00730 at 168.195 kb on - strand, within DZA65_RS00730 at 168.195 kb on - strand, within DZA65_RS00730 at 168.300 kb on + strand, within DZA65_RS00730 at 168.300 kb on + strand, within DZA65_RS00730 at 168.300 kb on + strand, within DZA65_RS00730 at 168.300 kb on + strand, within DZA65_RS00730 at 168.300 kb on + strand, within DZA65_RS00730 at 168.300 kb on + strand, within DZA65_RS00730 at 168.301 kb on - strand, within DZA65_RS00730 at 168.301 kb on - strand, within DZA65_RS00730 at 168.486 kb on + strand at 168.522 kb on + strand at 168.522 kb on + strand at 168.522 kb on + strand at 168.522 kb on + strand at 168.523 kb on - strand at 168.523 kb on - strand at 168.523 kb on - strand at 168.524 kb on + strand at 168.538 kb on + strand at 168.539 kb on - strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.543 kb on + strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.544 kb on - strand at 168.586 kb on - strand at 168.611 kb on - strand at 168.611 kb on - strand at 168.650 kb on + strand at 168.669 kb on + strand, within DZA65_RS00735 at 168.669 kb on + strand, within DZA65_RS00735 at 168.687 kb on + strand, within DZA65_RS00735 at 168.688 kb on - strand, within DZA65_RS00735 at 168.697 kb on + strand, within DZA65_RS00735 at 168.720 kb on + strand, within DZA65_RS00735 at 168.720 kb on + strand, within DZA65_RS00735 at 168.720 kb on + strand, within DZA65_RS00735 at 168.721 kb on - strand, within DZA65_RS00735 at 168.721 kb on - strand, within DZA65_RS00735 at 168.721 kb on - strand, within DZA65_RS00735 at 168.723 kb on + strand, within DZA65_RS00735 at 168.723 kb on + strand, within DZA65_RS00735 at 168.758 kb on - strand, within DZA65_RS00735 at 168.758 kb on - strand, within DZA65_RS00735 at 168.814 kb on + strand, within DZA65_RS00735 at 168.814 kb on + strand, within DZA65_RS00735 at 168.815 kb on - strand, within DZA65_RS00735 at 168.883 kb on + strand, within DZA65_RS00735 at 169.120 kb on + strand, within DZA65_RS00735 at 169.260 kb on - strand at 169.292 kb on + strand at 169.293 kb on - strand at 169.472 kb on - strand, within DZA65_RS00740 at 169.656 kb on + strand, within DZA65_RS00740 at 169.656 kb on + strand, within DZA65_RS00740 at 169.657 kb on - strand, within DZA65_RS00740 at 169.657 kb on - strand, within DZA65_RS00740 at 169.657 kb on - strand, within DZA65_RS00740 at 169.870 kb on - strand at 169.963 kb on + strand at 169.963 kb on + strand at 169.963 kb on + strand at 169.963 kb on + strand at 169.963 kb on + strand at 169.963 kb on + strand at 169.964 kb on - strand at 169.964 kb on - strand at 169.964 kb on - strand at 169.965 kb on + strand at 169.965 kb on + strand at 169.966 kb on - strand at 169.966 kb on - strand at 169.966 kb on - strand at 169.966 kb on - strand at 169.966 kb on - strand at 169.995 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 167,626 + -0.2 167,626 + -1.0 167,626 + -0.4 167,626 + -0.6 167,626 + -0.6 167,626 + -0.5 167,627 - +0.6 167,627 - -0.0 167,627 - +0.5 167,627 - +0.4 167,627 - -1.8 167,627 - +1.1 167,627 - +0.5 167,627 - -2.5 167,627 - -0.3 167,627 - +0.3 167,691 + +0.4 167,730 + +0.7 167,730 + -0.4 167,730 + +0.1 167,730 + -2.6 167,730 + -0.1 167,730 + +0.2 167,730 + -1.5 167,730 + +0.3 167,731 - -0.2 167,731 - -0.2 167,731 - -0.4 167,731 - +2.2 167,731 - -0.4 167,731 - -1.0 167,731 - -0.4 167,731 - -0.2 167,732 + +0.9 167,732 + -1.6 167,732 + -1.6 167,732 + +0.1 167,732 + -0.0 167,732 + +0.4 167,732 + +0.9 167,732 + -0.6 167,732 + -2.4 167,733 - -0.2 167,733 - +1.2 167,747 + -0.3 167,748 - -1.1 167,873 + DZA65_RS00730 0.14 +0.0 167,958 + DZA65_RS00730 0.26 +0.5 167,958 + DZA65_RS00730 0.26 +0.3 167,959 - -1.0 167,963 + DZA65_RS00730 0.27 +0.0 167,963 + DZA65_RS00730 0.27 -0.2 167,963 + DZA65_RS00730 0.27 -0.5 167,963 + DZA65_RS00730 0.27 +2.0 167,964 - DZA65_RS00730 0.27 -2.5 168,099 + +0.4 168,099 + DZA65_RS00730 0.46 -1.0 168,099 + DZA65_RS00730 0.46 -0.4 168,099 + DZA65_RS00730 0.46 +1.6 168,099 + DZA65_RS00730 0.46 -0.6 168,099 + DZA65_RS00730 0.46 -2.0 168,099 + DZA65_RS00730 0.46 -2.0 168,099 + DZA65_RS00730 0.46 -0.1 168,099 + DZA65_RS00730 0.46 +0.8 168,099 + DZA65_RS00730 0.46 -1.8 168,099 + DZA65_RS00730 0.46 +0.5 168,100 - DZA65_RS00730 0.46 -1.3 168,100 - DZA65_RS00730 0.46 -2.0 168,100 - DZA65_RS00730 0.46 +0.0 168,100 - DZA65_RS00730 0.46 -1.6 168,100 - DZA65_RS00730 0.46 -0.2 168,100 - DZA65_RS00730 0.46 -1.5 168,107 + DZA65_RS00730 0.47 -0.2 168,108 - DZA65_RS00730 0.47 +0.1 168,108 - DZA65_RS00730 0.47 -0.4 168,195 - DZA65_RS00730 0.59 -0.2 168,195 - DZA65_RS00730 0.59 +0.4 168,300 + DZA65_RS00730 0.74 +1.9 168,300 + DZA65_RS00730 0.74 -0.6 168,300 + DZA65_RS00730 0.74 +0.8 168,300 + DZA65_RS00730 0.74 -0.5 168,300 + DZA65_RS00730 0.74 +0.9 168,300 + DZA65_RS00730 0.74 +1.1 168,301 - DZA65_RS00730 0.74 +0.1 168,301 - DZA65_RS00730 0.74 -1.5 168,486 + -1.2 168,522 + +1.7 168,522 + +0.8 168,522 + -0.1 168,522 + +0.4 168,523 - -0.2 168,523 - -0.3 168,523 - -0.1 168,524 + +0.5 168,538 + -0.5 168,539 - -0.0 168,543 + -1.2 168,543 + -2.0 168,543 + +0.1 168,543 + -1.0 168,543 + -1.7 168,543 + -1.1 168,543 + -0.4 168,543 + +1.2 168,543 + -1.0 168,543 + -1.8 168,543 + -0.7 168,543 + -0.4 168,543 + +0.4 168,543 + +1.2 168,543 + -1.2 168,543 + +1.7 168,543 + -2.1 168,543 + -0.8 168,543 + +0.5 168,543 + -0.3 168,543 + -0.2 168,543 + -0.3 168,543 + -0.5 168,543 + +1.0 168,543 + -0.9 168,544 - -1.2 168,544 - +0.8 168,544 - +2.9 168,544 - -1.5 168,544 - +0.1 168,544 - -0.9 168,544 - +0.0 168,544 - -1.6 168,544 - -0.4 168,544 - +0.7 168,544 - -1.6 168,544 - -0.8 168,544 - -1.2 168,544 - +2.1 168,544 - -1.2 168,544 - -0.1 168,544 - -0.3 168,544 - -1.0 168,544 - -2.2 168,544 - -0.6 168,544 - -1.3 168,544 - -1.5 168,544 - -0.5 168,544 - +0.0 168,544 - -1.0 168,544 - -0.1 168,544 - +0.8 168,544 - +0.6 168,544 - +0.0 168,544 - +0.7 168,586 - +0.4 168,611 - -3.6 168,611 - -1.2 168,650 + -1.8 168,669 + DZA65_RS00735 0.11 +0.3 168,669 + DZA65_RS00735 0.11 -3.0 168,687 + DZA65_RS00735 0.13 -2.2 168,688 - DZA65_RS00735 0.14 -3.0 168,697 + DZA65_RS00735 0.15 -3.0 168,720 + DZA65_RS00735 0.19 -1.6 168,720 + DZA65_RS00735 0.19 -1.2 168,720 + DZA65_RS00735 0.19 -3.5 168,721 - DZA65_RS00735 0.19 -2.6 168,721 - DZA65_RS00735 0.19 -2.2 168,721 - DZA65_RS00735 0.19 -2.5 168,723 + DZA65_RS00735 0.19 -2.9 168,723 + DZA65_RS00735 0.19 -2.0 168,758 - DZA65_RS00735 0.24 -2.5 168,758 - DZA65_RS00735 0.24 -2.1 168,814 + DZA65_RS00735 0.33 -1.5 168,814 + DZA65_RS00735 0.33 -2.6 168,815 - DZA65_RS00735 0.33 -1.2 168,883 + DZA65_RS00735 0.44 -2.3 169,120 + DZA65_RS00735 0.81 -1.8 169,260 - +0.6 169,292 + +0.6 169,293 - +1.6 169,472 - DZA65_RS00740 0.30 +1.0 169,656 + DZA65_RS00740 0.61 +1.7 169,656 + DZA65_RS00740 0.61 -1.1 169,657 - DZA65_RS00740 0.61 -1.8 169,657 - DZA65_RS00740 0.61 +1.8 169,657 - DZA65_RS00740 0.61 +1.9 169,870 - -2.5 169,963 + -0.0 169,963 + -0.6 169,963 + -0.5 169,963 + -1.2 169,963 + -0.6 169,963 + -0.0 169,964 - -2.5 169,964 - -0.1 169,964 - +1.4 169,965 + +0.1 169,965 + +0.0 169,966 - -0.5 169,966 - -1.1 169,966 - -1.1 169,966 - +1.8 169,966 - +1.1 169,995 - -1.5
Or see this region's nucleotide sequence