Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS00670 and DZA65_RS00675 overlap by 19 nucleotides DZA65_RS00675 and DZA65_RS00680 are separated by 27 nucleotides DZA65_RS00680 and DZA65_RS00685 are separated by 32 nucleotides
DZA65_RS00670: DZA65_RS00670 - HlyD family efflux transporter periplasmic adaptor subunit, at 154,920 to 156,182
_RS00670
DZA65_RS00675: DZA65_RS00675 - isoaspartyl peptidase/L-asparaginase, at 156,164 to 157,132
_RS00675
DZA65_RS00680: DZA65_RS00680 - M55 family metallopeptidase, at 157,160 to 157,981
_RS00680
DZA65_RS00685: DZA65_RS00685 - P1 family peptidase, at 158,014 to 159,069
_RS00685
Position (kb)
157
158 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 156.169 kb on + strand at 156.169 kb on + strand at 156.170 kb on - strand at 156.181 kb on - strand at 156.181 kb on - strand at 156.210 kb on + strand at 156.211 kb on - strand at 156.225 kb on + strand at 156.225 kb on + strand at 156.225 kb on + strand at 156.226 kb on - strand at 156.226 kb on - strand at 156.384 kb on + strand, within DZA65_RS00675 at 156.385 kb on - strand, within DZA65_RS00675 at 156.385 kb on - strand, within DZA65_RS00675 at 156.457 kb on - strand, within DZA65_RS00675 at 156.457 kb on - strand, within DZA65_RS00675 at 157.120 kb on + strand at 157.145 kb on + strand at 157.145 kb on + strand at 157.145 kb on + strand at 157.145 kb on + strand at 157.146 kb on - strand at 157.162 kb on - strand at 157.162 kb on - strand at 157.162 kb on - strand at 157.209 kb on - strand at 157.327 kb on - strand at 157.357 kb on + strand, within DZA65_RS00680 at 157.467 kb on + strand, within DZA65_RS00680 at 157.467 kb on + strand, within DZA65_RS00680 at 157.467 kb on + strand, within DZA65_RS00680 at 157.467 kb on + strand, within DZA65_RS00680 at 157.468 kb on - strand, within DZA65_RS00680 at 157.468 kb on - strand, within DZA65_RS00680 at 157.468 kb on - strand, within DZA65_RS00680 at 157.468 kb on - strand, within DZA65_RS00680 at 157.468 kb on - strand, within DZA65_RS00680 at 157.567 kb on - strand, within DZA65_RS00680 at 157.571 kb on - strand, within DZA65_RS00680 at 157.571 kb on - strand, within DZA65_RS00680 at 157.611 kb on + strand, within DZA65_RS00680 at 157.611 kb on + strand, within DZA65_RS00680 at 157.772 kb on - strand at 157.892 kb on + strand at 157.899 kb on + strand at 157.900 kb on - strand at 157.900 kb on - strand at 157.970 kb on - strand at 157.970 kb on - strand at 157.973 kb on + strand at 157.974 kb on - strand at 157.974 kb on - strand at 157.974 kb on - strand at 158.326 kb on - strand, within DZA65_RS00685 at 158.626 kb on - strand, within DZA65_RS00685 at 158.632 kb on - strand, within DZA65_RS00685 at 158.731 kb on - strand, within DZA65_RS00685 at 158.803 kb on + strand, within DZA65_RS00685 at 158.865 kb on + strand, within DZA65_RS00685 at 158.865 kb on + strand, within DZA65_RS00685 at 158.931 kb on + strand, within DZA65_RS00685 at 158.931 kb on + strand, within DZA65_RS00685 at 158.931 kb on + strand, within DZA65_RS00685 at 158.932 kb on - strand, within DZA65_RS00685 at 158.932 kb on - strand, within DZA65_RS00685 at 158.932 kb on - strand, within DZA65_RS00685 at 158.932 kb on - strand, within DZA65_RS00685 at 158.957 kb on - strand, within DZA65_RS00685
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 156,169 + -1.5 156,169 + +1.5 156,170 - +0.3 156,181 - +1.1 156,181 - -1.0 156,210 + -1.2 156,211 - -0.6 156,225 + -0.5 156,225 + -0.5 156,225 + +0.0 156,226 - -1.5 156,226 - -0.2 156,384 + DZA65_RS00675 0.23 -1.8 156,385 - DZA65_RS00675 0.23 -0.2 156,385 - DZA65_RS00675 0.23 -0.1 156,457 - DZA65_RS00675 0.30 -0.8 156,457 - DZA65_RS00675 0.30 +0.5 157,120 + +0.9 157,145 + -1.8 157,145 + +0.4 157,145 + +1.4 157,145 + -1.1 157,146 - +0.2 157,162 - -1.2 157,162 - +0.3 157,162 - +2.4 157,209 - +0.9 157,327 - +0.4 157,357 + DZA65_RS00680 0.24 -1.2 157,467 + DZA65_RS00680 0.37 -1.8 157,467 + DZA65_RS00680 0.37 -0.9 157,467 + DZA65_RS00680 0.37 +0.4 157,467 + DZA65_RS00680 0.37 -0.3 157,468 - DZA65_RS00680 0.37 -0.6 157,468 - DZA65_RS00680 0.37 -1.1 157,468 - DZA65_RS00680 0.37 -1.0 157,468 - DZA65_RS00680 0.37 -1.1 157,468 - DZA65_RS00680 0.37 -1.7 157,567 - DZA65_RS00680 0.50 -1.3 157,571 - DZA65_RS00680 0.50 -0.5 157,571 - DZA65_RS00680 0.50 +0.4 157,611 + DZA65_RS00680 0.55 -0.4 157,611 + DZA65_RS00680 0.55 -0.6 157,772 - +1.4 157,892 + -0.6 157,899 + -0.1 157,900 - +0.5 157,900 - -1.5 157,970 - +0.4 157,970 - -1.5 157,973 + -1.0 157,974 - +0.7 157,974 - -0.1 157,974 - +0.0 158,326 - DZA65_RS00685 0.30 +0.5 158,626 - DZA65_RS00685 0.58 +1.0 158,632 - DZA65_RS00685 0.59 +1.0 158,731 - DZA65_RS00685 0.68 -0.5 158,803 + DZA65_RS00685 0.75 -0.2 158,865 + DZA65_RS00685 0.81 -1.6 158,865 + DZA65_RS00685 0.81 -1.6 158,931 + DZA65_RS00685 0.87 +0.1 158,931 + DZA65_RS00685 0.87 +0.0 158,931 + DZA65_RS00685 0.87 -0.7 158,932 - DZA65_RS00685 0.87 -0.6 158,932 - DZA65_RS00685 0.87 +0.4 158,932 - DZA65_RS00685 0.87 -0.1 158,932 - DZA65_RS00685 0.87 +1.8 158,957 - DZA65_RS00685 0.89 -1.3
Or see this region's nucleotide sequence