Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS00520 and DZA65_RS00525 are separated by 94 nucleotides DZA65_RS00525 and DZA65_RS00530 are separated by 122 nucleotides DZA65_RS00530 and DZA65_RS00535 are separated by 337 nucleotides DZA65_RS00535 and DZA65_RS00540 are separated by 98 nucleotides
DZA65_RS00520: DZA65_RS00520 - acyl-homoserine-lactone synthase, at 123,033 to 123,671
_RS00520
DZA65_RS00525: DZA65_RS00525 - hypothetical protein, at 123,766 to 123,993
_RS00525
DZA65_RS00530: DZA65_RS00530 - DUF1471 domain-containing protein, at 124,116 to 124,364
_RS00530
DZA65_RS00535: DZA65_RS00535 - helix-turn-helix transcriptional regulator, at 124,702 to 125,055
_RS00535
DZA65_RS00540: DZA65_RS00540 - transcriptional regulator, at 125,154 to 125,411
_RS00540
Position (kb) 124
125 Strain fitness (log2 ratio) -2
-1
0
1
2 at 123.219 kb on + strand, within DZA65_RS00520 at 123.219 kb on + strand, within DZA65_RS00520 at 123.265 kb on + strand, within DZA65_RS00520 at 123.265 kb on + strand, within DZA65_RS00520 at 123.385 kb on + strand, within DZA65_RS00520 at 123.386 kb on - strand, within DZA65_RS00520 at 123.386 kb on - strand, within DZA65_RS00520 at 123.476 kb on + strand, within DZA65_RS00520 at 123.477 kb on - strand, within DZA65_RS00520 at 123.511 kb on + strand, within DZA65_RS00520 at 123.511 kb on + strand, within DZA65_RS00520 at 123.532 kb on + strand, within DZA65_RS00520 at 123.533 kb on - strand, within DZA65_RS00520 at 123.533 kb on - strand, within DZA65_RS00520 at 123.587 kb on + strand, within DZA65_RS00520 at 123.604 kb on - strand, within DZA65_RS00520 at 123.608 kb on + strand at 123.609 kb on - strand at 123.609 kb on - strand at 123.609 kb on - strand at 123.630 kb on - strand at 123.639 kb on + strand at 123.693 kb on + strand at 123.693 kb on + strand at 123.693 kb on + strand at 123.694 kb on - strand at 123.730 kb on - strand at 123.730 kb on - strand at 123.768 kb on - strand at 123.768 kb on - strand at 123.771 kb on + strand at 123.793 kb on - strand, within DZA65_RS00525 at 123.801 kb on + strand, within DZA65_RS00525 at 123.802 kb on - strand, within DZA65_RS00525 at 123.811 kb on - strand, within DZA65_RS00525 at 123.811 kb on - strand, within DZA65_RS00525 at 123.813 kb on + strand, within DZA65_RS00525 at 123.813 kb on + strand, within DZA65_RS00525 at 123.814 kb on - strand, within DZA65_RS00525 at 123.814 kb on - strand, within DZA65_RS00525 at 123.814 kb on - strand, within DZA65_RS00525 at 123.852 kb on + strand, within DZA65_RS00525 at 123.853 kb on - strand, within DZA65_RS00525 at 123.853 kb on - strand, within DZA65_RS00525 at 123.853 kb on - strand, within DZA65_RS00525 at 123.884 kb on - strand, within DZA65_RS00525 at 123.884 kb on - strand, within DZA65_RS00525 at 123.900 kb on + strand, within DZA65_RS00525 at 123.901 kb on - strand, within DZA65_RS00525 at 123.957 kb on - strand, within DZA65_RS00525 at 123.957 kb on - strand, within DZA65_RS00525 at 123.957 kb on - strand, within DZA65_RS00525 at 123.957 kb on - strand, within DZA65_RS00525 at 123.978 kb on + strand at 123.979 kb on - strand at 124.005 kb on - strand at 124.043 kb on + strand at 124.103 kb on - strand at 124.115 kb on + strand at 124.115 kb on + strand at 124.115 kb on + strand at 124.115 kb on + strand at 124.116 kb on - strand at 124.116 kb on - strand at 124.116 kb on - strand at 124.116 kb on - strand at 124.116 kb on - strand at 124.116 kb on - strand at 124.116 kb on - strand at 124.124 kb on + strand at 124.124 kb on + strand at 124.124 kb on + strand at 124.124 kb on + strand at 124.124 kb on + strand at 124.125 kb on - strand at 124.309 kb on + strand, within DZA65_RS00530 at 124.358 kb on + strand at 124.358 kb on + strand at 124.358 kb on + strand at 124.359 kb on - strand at 124.359 kb on - strand at 124.362 kb on + strand at 124.362 kb on + strand at 124.362 kb on + strand at 124.362 kb on + strand at 124.363 kb on - strand at 124.363 kb on - strand at 124.363 kb on - strand at 124.363 kb on - strand at 124.363 kb on - strand at 124.416 kb on + strand at 124.435 kb on + strand at 124.440 kb on + strand at 124.440 kb on + strand at 124.440 kb on + strand at 124.440 kb on + strand at 124.440 kb on + strand at 124.441 kb on - strand at 124.441 kb on - strand at 124.499 kb on + strand at 124.502 kb on + strand at 124.526 kb on + strand at 124.526 kb on + strand at 124.527 kb on - strand at 124.530 kb on + strand at 124.530 kb on + strand at 124.530 kb on + strand at 124.530 kb on + strand at 124.531 kb on - strand at 124.531 kb on - strand at 124.531 kb on - strand at 124.531 kb on - strand at 124.531 kb on - strand at 124.554 kb on + strand at 124.554 kb on + strand at 124.554 kb on + strand at 124.617 kb on - strand at 124.635 kb on - strand at 124.636 kb on + strand at 124.636 kb on + strand at 124.637 kb on - strand at 124.637 kb on - strand at 124.650 kb on + strand at 124.685 kb on + strand at 124.685 kb on + strand at 124.686 kb on - strand at 124.703 kb on + strand at 124.703 kb on + strand at 124.835 kb on + strand, within DZA65_RS00535 at 124.835 kb on + strand, within DZA65_RS00535 at 124.836 kb on - strand, within DZA65_RS00535 at 124.924 kb on - strand, within DZA65_RS00535 at 124.932 kb on + strand, within DZA65_RS00535 at 124.933 kb on - strand, within DZA65_RS00535 at 125.010 kb on + strand, within DZA65_RS00535 at 125.010 kb on + strand, within DZA65_RS00535 at 125.011 kb on - strand, within DZA65_RS00535 at 125.037 kb on - strand at 125.037 kb on - strand at 125.104 kb on + strand at 125.129 kb on + strand at 125.130 kb on - strand at 125.130 kb on - strand at 125.180 kb on + strand, within DZA65_RS00540 at 125.180 kb on + strand, within DZA65_RS00540 at 125.180 kb on + strand, within DZA65_RS00540 at 125.180 kb on + strand, within DZA65_RS00540 at 125.181 kb on - strand, within DZA65_RS00540 at 125.181 kb on - strand, within DZA65_RS00540 at 125.181 kb on - strand, within DZA65_RS00540 at 125.181 kb on - strand, within DZA65_RS00540 at 125.181 kb on - strand, within DZA65_RS00540 at 125.181 kb on - strand, within DZA65_RS00540 at 125.181 kb on - strand, within DZA65_RS00540 at 125.181 kb on - strand, within DZA65_RS00540 at 125.207 kb on + strand, within DZA65_RS00540 at 125.207 kb on + strand, within DZA65_RS00540 at 125.207 kb on + strand, within DZA65_RS00540 at 125.207 kb on + strand, within DZA65_RS00540 at 125.207 kb on + strand, within DZA65_RS00540 at 125.207 kb on + strand, within DZA65_RS00540 at 125.207 kb on + strand, within DZA65_RS00540 at 125.208 kb on - strand, within DZA65_RS00540 at 125.208 kb on - strand, within DZA65_RS00540 at 125.208 kb on - strand, within DZA65_RS00540 at 125.335 kb on + strand, within DZA65_RS00540 at 125.350 kb on - strand, within DZA65_RS00540 at 125.352 kb on + strand, within DZA65_RS00540 at 125.352 kb on + strand, within DZA65_RS00540 at 125.352 kb on + strand, within DZA65_RS00540 at 125.352 kb on + strand, within DZA65_RS00540 at 125.352 kb on + strand, within DZA65_RS00540 at 125.352 kb on + strand, within DZA65_RS00540 at 125.353 kb on - strand, within DZA65_RS00540 at 125.353 kb on - strand, within DZA65_RS00540 at 125.353 kb on - strand, within DZA65_RS00540 at 125.353 kb on - strand, within DZA65_RS00540
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 123,219 + DZA65_RS00520 0.29 +0.7 123,219 + DZA65_RS00520 0.29 +0.3 123,265 + DZA65_RS00520 0.36 +0.4 123,265 + DZA65_RS00520 0.36 +0.8 123,385 + DZA65_RS00520 0.55 -0.2 123,386 - DZA65_RS00520 0.55 -0.2 123,386 - DZA65_RS00520 0.55 -1.8 123,476 + DZA65_RS00520 0.69 -1.1 123,477 - DZA65_RS00520 0.69 -1.4 123,511 + DZA65_RS00520 0.75 -0.6 123,511 + DZA65_RS00520 0.75 +1.2 123,532 + DZA65_RS00520 0.78 -1.0 123,533 - DZA65_RS00520 0.78 -0.1 123,533 - DZA65_RS00520 0.78 -0.3 123,587 + DZA65_RS00520 0.87 -0.4 123,604 - DZA65_RS00520 0.89 +0.4 123,608 + -0.4 123,609 - -0.6 123,609 - +0.2 123,609 - +0.0 123,630 - -0.2 123,639 + +0.9 123,693 + -0.8 123,693 + -0.3 123,693 + -0.4 123,694 - -0.6 123,730 - -1.7 123,730 - -1.3 123,768 - -0.1 123,768 - -0.4 123,771 + +0.9 123,793 - DZA65_RS00525 0.12 -2.0 123,801 + DZA65_RS00525 0.15 -0.4 123,802 - DZA65_RS00525 0.16 -0.7 123,811 - DZA65_RS00525 0.20 +0.0 123,811 - DZA65_RS00525 0.20 -0.4 123,813 + DZA65_RS00525 0.21 -1.2 123,813 + DZA65_RS00525 0.21 -0.6 123,814 - DZA65_RS00525 0.21 +0.4 123,814 - DZA65_RS00525 0.21 -1.5 123,814 - DZA65_RS00525 0.21 -1.4 123,852 + DZA65_RS00525 0.38 -0.8 123,853 - DZA65_RS00525 0.38 -1.6 123,853 - DZA65_RS00525 0.38 +0.2 123,853 - DZA65_RS00525 0.38 -2.1 123,884 - DZA65_RS00525 0.52 -0.2 123,884 - DZA65_RS00525 0.52 -0.8 123,900 + DZA65_RS00525 0.59 -1.1 123,901 - DZA65_RS00525 0.59 +0.5 123,957 - DZA65_RS00525 0.84 +0.6 123,957 - DZA65_RS00525 0.84 -0.0 123,957 - DZA65_RS00525 0.84 +2.4 123,957 - DZA65_RS00525 0.84 +0.4 123,978 + -0.8 123,979 - -1.8 124,005 - -0.6 124,043 + -0.1 124,103 - -0.1 124,115 + -0.6 124,115 + +1.1 124,115 + +0.1 124,115 + -1.1 124,116 - +0.6 124,116 - -1.1 124,116 - +0.4 124,116 - -1.2 124,116 - +0.7 124,116 - -0.5 124,116 - +0.9 124,124 + +0.6 124,124 + -1.0 124,124 + -1.1 124,124 + +0.4 124,124 + +0.4 124,125 - +1.6 124,309 + DZA65_RS00530 0.78 -1.5 124,358 + -0.6 124,358 + +1.1 124,358 + -2.6 124,359 - +1.9 124,359 - -1.0 124,362 + +0.8 124,362 + -0.5 124,362 + -0.2 124,362 + -0.8 124,363 - -0.1 124,363 - -0.2 124,363 - +1.1 124,363 - +0.4 124,363 - -1.0 124,416 + +1.0 124,435 + -1.0 124,440 + -0.5 124,440 + -1.1 124,440 + -0.5 124,440 + -0.4 124,440 + -0.6 124,441 - -1.2 124,441 - -1.6 124,499 + -0.5 124,502 + -0.2 124,526 + +0.0 124,526 + +0.2 124,527 - +0.4 124,530 + +1.1 124,530 + -1.2 124,530 + +0.7 124,530 + -0.2 124,531 - -0.5 124,531 - -2.1 124,531 - +0.2 124,531 - -0.6 124,531 - -0.1 124,554 + +0.8 124,554 + -0.2 124,554 + -1.3 124,617 - -0.2 124,635 - -0.1 124,636 + -0.2 124,636 + -1.5 124,637 - -0.1 124,637 - -0.1 124,650 + +0.1 124,685 + +2.0 124,685 + -0.2 124,686 - +0.5 124,703 + -0.5 124,703 + -2.1 124,835 + DZA65_RS00535 0.38 +1.4 124,835 + DZA65_RS00535 0.38 +2.2 124,836 - DZA65_RS00535 0.38 -1.6 124,924 - DZA65_RS00535 0.63 -0.5 124,932 + DZA65_RS00535 0.65 +0.4 124,933 - DZA65_RS00535 0.65 +1.4 125,010 + DZA65_RS00535 0.87 -1.0 125,010 + DZA65_RS00535 0.87 +0.4 125,011 - DZA65_RS00535 0.87 -1.5 125,037 - +0.1 125,037 - -0.3 125,104 + -1.0 125,129 + -0.0 125,130 - +0.2 125,130 - -1.8 125,180 + DZA65_RS00540 0.10 +0.8 125,180 + DZA65_RS00540 0.10 +0.1 125,180 + DZA65_RS00540 0.10 -0.2 125,180 + DZA65_RS00540 0.10 +0.4 125,181 - DZA65_RS00540 0.10 -0.6 125,181 - DZA65_RS00540 0.10 -0.4 125,181 - DZA65_RS00540 0.10 +0.4 125,181 - DZA65_RS00540 0.10 +0.5 125,181 - DZA65_RS00540 0.10 +0.0 125,181 - DZA65_RS00540 0.10 -0.5 125,181 - DZA65_RS00540 0.10 +0.4 125,181 - DZA65_RS00540 0.10 -0.3 125,207 + DZA65_RS00540 0.21 -0.1 125,207 + DZA65_RS00540 0.21 +0.4 125,207 + DZA65_RS00540 0.21 -0.3 125,207 + DZA65_RS00540 0.21 +1.4 125,207 + DZA65_RS00540 0.21 -0.2 125,207 + DZA65_RS00540 0.21 -1.8 125,207 + DZA65_RS00540 0.21 -0.2 125,208 - DZA65_RS00540 0.21 +0.4 125,208 - DZA65_RS00540 0.21 +0.7 125,208 - DZA65_RS00540 0.21 -0.9 125,335 + DZA65_RS00540 0.70 -0.1 125,350 - DZA65_RS00540 0.76 +0.4 125,352 + DZA65_RS00540 0.77 -0.6 125,352 + DZA65_RS00540 0.77 -0.7 125,352 + DZA65_RS00540 0.77 -0.6 125,352 + DZA65_RS00540 0.77 +0.4 125,352 + DZA65_RS00540 0.77 -0.2 125,352 + DZA65_RS00540 0.77 -2.0 125,353 - DZA65_RS00540 0.77 +0.1 125,353 - DZA65_RS00540 0.77 -0.2 125,353 - DZA65_RS00540 0.77 +1.0 125,353 - DZA65_RS00540 0.77 -0.2
Or see this region's nucleotide sequence