Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS17145 and DZA65_RS17150 overlap by 4 nucleotides DZA65_RS17150 and DZA65_RS17155 are separated by 160 nucleotides DZA65_RS17155 and DZA65_RS17160 are separated by 15 nucleotides
DZA65_RS17145: DZA65_RS17145 - polynucleotide adenylyltransferase PcnB, at 3,703,398 to 3,704,801
_RS17145
DZA65_RS17150: DZA65_RS17150 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,704,798 to 3,705,286
_RS17150
DZA65_RS17155: DZA65_RS17155 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,705,447 to 3,706,241
_RS17155
DZA65_RS17160: DZA65_RS17160 - pantoate--beta-alanine ligase, at 3,706,257 to 3,707,111
_RS17160
Position (kb)
3704
3705
3706 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3703.832 kb on + strand at 3703.833 kb on - strand, within DZA65_RS17145 at 3703.911 kb on + strand, within DZA65_RS17145 at 3703.941 kb on + strand, within DZA65_RS17145 at 3704.190 kb on + strand, within DZA65_RS17145 at 3704.190 kb on + strand, within DZA65_RS17145 at 3704.208 kb on + strand, within DZA65_RS17145 at 3704.248 kb on - strand, within DZA65_RS17145 at 3704.424 kb on + strand, within DZA65_RS17145 at 3704.444 kb on - strand, within DZA65_RS17145 at 3704.501 kb on + strand, within DZA65_RS17145 at 3704.571 kb on - strand, within DZA65_RS17145 at 3704.650 kb on + strand, within DZA65_RS17145 at 3704.651 kb on - strand, within DZA65_RS17145 at 3704.651 kb on - strand, within DZA65_RS17145 at 3704.651 kb on - strand, within DZA65_RS17145 at 3704.810 kb on + strand at 3705.335 kb on - strand at 3705.362 kb on - strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.398 kb on + strand at 3705.398 kb on + strand at 3705.398 kb on + strand at 3705.398 kb on + strand at 3705.398 kb on + strand at 3705.398 kb on + strand at 3705.414 kb on + strand at 3705.446 kb on + strand at 3705.446 kb on + strand at 3705.447 kb on - strand at 3705.447 kb on - strand at 3705.447 kb on - strand at 3705.519 kb on + strand at 3705.519 kb on + strand at 3705.520 kb on - strand at 3705.546 kb on + strand, within DZA65_RS17155 at 3705.546 kb on + strand, within DZA65_RS17155 at 3705.547 kb on - strand, within DZA65_RS17155 at 3705.591 kb on - strand, within DZA65_RS17155 at 3705.598 kb on + strand, within DZA65_RS17155 at 3705.638 kb on + strand, within DZA65_RS17155 at 3705.639 kb on - strand, within DZA65_RS17155 at 3705.646 kb on - strand, within DZA65_RS17155 at 3705.661 kb on + strand, within DZA65_RS17155 at 3705.708 kb on - strand, within DZA65_RS17155 at 3705.773 kb on + strand, within DZA65_RS17155 at 3705.773 kb on + strand, within DZA65_RS17155 at 3705.774 kb on - strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.785 kb on - strand, within DZA65_RS17155 at 3705.785 kb on - strand, within DZA65_RS17155 at 3705.785 kb on - strand, within DZA65_RS17155 at 3705.785 kb on - strand, within DZA65_RS17155 at 3705.882 kb on - strand, within DZA65_RS17155 at 3705.882 kb on - strand, within DZA65_RS17155 at 3705.882 kb on - strand, within DZA65_RS17155 at 3705.882 kb on - strand, within DZA65_RS17155 at 3705.896 kb on + strand, within DZA65_RS17155 at 3705.976 kb on + strand, within DZA65_RS17155 at 3706.121 kb on + strand, within DZA65_RS17155 at 3706.185 kb on + strand at 3706.185 kb on + strand at 3706.185 kb on + strand at 3706.185 kb on + strand at 3706.185 kb on + strand at 3706.185 kb on + strand at 3706.185 kb on + strand at 3706.185 kb on + strand at 3706.186 kb on - strand at 3706.186 kb on - strand at 3706.186 kb on - strand at 3706.186 kb on - strand at 3706.227 kb on + strand at 3706.227 kb on + strand at 3706.228 kb on - strand at 3706.228 kb on - strand at 3706.239 kb on + strand at 3706.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,703,832 + -1.0 3,703,833 - DZA65_RS17145 0.31 +0.7 3,703,911 + DZA65_RS17145 0.37 +0.7 3,703,941 + DZA65_RS17145 0.39 -0.8 3,704,190 + DZA65_RS17145 0.56 +0.7 3,704,190 + DZA65_RS17145 0.56 -1.0 3,704,208 + DZA65_RS17145 0.58 +0.9 3,704,248 - DZA65_RS17145 0.61 +1.0 3,704,424 + DZA65_RS17145 0.73 -0.8 3,704,444 - DZA65_RS17145 0.75 +0.0 3,704,501 + DZA65_RS17145 0.79 +1.3 3,704,571 - DZA65_RS17145 0.84 -0.5 3,704,650 + DZA65_RS17145 0.89 +0.9 3,704,651 - DZA65_RS17145 0.89 +1.5 3,704,651 - DZA65_RS17145 0.89 -1.5 3,704,651 - DZA65_RS17145 0.89 -2.7 3,704,810 + -0.3 3,705,335 - +0.7 3,705,362 - -0.2 3,705,395 + +0.2 3,705,395 + -0.5 3,705,395 + +0.6 3,705,395 + -0.2 3,705,395 + -0.2 3,705,395 + +0.7 3,705,395 + -1.8 3,705,395 + -0.2 3,705,395 + +0.2 3,705,395 + -1.0 3,705,395 + -0.5 3,705,395 + -2.0 3,705,396 - +0.0 3,705,396 - -1.9 3,705,396 - -1.4 3,705,396 - -1.1 3,705,396 - -0.8 3,705,396 - -2.0 3,705,396 - -1.0 3,705,396 - -2.3 3,705,398 + -1.1 3,705,398 + +1.1 3,705,398 + -0.7 3,705,398 + -1.3 3,705,398 + -0.5 3,705,398 + +1.6 3,705,414 + -1.5 3,705,446 + -1.7 3,705,446 + -2.0 3,705,447 - -1.1 3,705,447 - -1.3 3,705,447 - -3.0 3,705,519 + +0.0 3,705,519 + +0.0 3,705,520 - -0.8 3,705,546 + DZA65_RS17155 0.12 -1.3 3,705,546 + DZA65_RS17155 0.12 -1.3 3,705,547 - DZA65_RS17155 0.13 +0.5 3,705,591 - DZA65_RS17155 0.18 -2.3 3,705,598 + DZA65_RS17155 0.19 -1.3 3,705,638 + DZA65_RS17155 0.24 -1.4 3,705,639 - DZA65_RS17155 0.24 -2.8 3,705,646 - DZA65_RS17155 0.25 -1.5 3,705,661 + DZA65_RS17155 0.27 -0.3 3,705,708 - DZA65_RS17155 0.33 -2.1 3,705,773 + DZA65_RS17155 0.41 -3.9 3,705,773 + DZA65_RS17155 0.41 -0.1 3,705,774 - DZA65_RS17155 0.41 -2.4 3,705,784 + DZA65_RS17155 0.42 -1.5 3,705,784 + DZA65_RS17155 0.42 -1.3 3,705,784 + DZA65_RS17155 0.42 -0.5 3,705,784 + DZA65_RS17155 0.42 -3.1 3,705,784 + DZA65_RS17155 0.42 -3.1 3,705,784 + DZA65_RS17155 0.42 -1.3 3,705,785 - DZA65_RS17155 0.43 -1.5 3,705,785 - DZA65_RS17155 0.43 -3.3 3,705,785 - DZA65_RS17155 0.43 -1.5 3,705,785 - DZA65_RS17155 0.43 -1.3 3,705,882 - DZA65_RS17155 0.55 -2.0 3,705,882 - DZA65_RS17155 0.55 -2.8 3,705,882 - DZA65_RS17155 0.55 -2.7 3,705,882 - DZA65_RS17155 0.55 -1.5 3,705,896 + DZA65_RS17155 0.56 +0.3 3,705,976 + DZA65_RS17155 0.67 -3.0 3,706,121 + DZA65_RS17155 0.85 -3.0 3,706,185 + -1.0 3,706,185 + -1.1 3,706,185 + -2.5 3,706,185 + -3.9 3,706,185 + -0.4 3,706,185 + +1.0 3,706,185 + -1.5 3,706,185 + -1.8 3,706,186 - -1.1 3,706,186 - -2.0 3,706,186 - -1.5 3,706,186 - -1.3 3,706,227 + -1.0 3,706,227 + -0.8 3,706,228 - -3.0 3,706,228 - -1.3 3,706,239 + -2.0 3,706,240 - -2.4
Or see this region's nucleotide sequence