Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS17140 and DZA65_RS17145 are separated by 68 nucleotides DZA65_RS17145 and DZA65_RS17150 overlap by 4 nucleotides DZA65_RS17150 and DZA65_RS17155 are separated by 160 nucleotides
DZA65_RS17140: DZA65_RS17140 - tRNA glutamyl-Q(34) synthetase GluQRS, at 3,702,421 to 3,703,329
_RS17140
DZA65_RS17145: DZA65_RS17145 - polynucleotide adenylyltransferase PcnB, at 3,703,398 to 3,704,801
_RS17145
DZA65_RS17150: DZA65_RS17150 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,704,798 to 3,705,286
_RS17150
DZA65_RS17155: DZA65_RS17155 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,705,447 to 3,706,241
_RS17155
Position (kb)
3703
3704
3705 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3702.413 kb on - strand at 3702.420 kb on + strand at 3702.420 kb on + strand at 3702.439 kb on + strand at 3702.439 kb on + strand at 3702.440 kb on - strand at 3702.440 kb on - strand at 3702.440 kb on - strand at 3702.440 kb on - strand at 3702.511 kb on + strand at 3702.511 kb on + strand at 3702.511 kb on + strand at 3702.511 kb on + strand at 3702.512 kb on - strand, within DZA65_RS17140 at 3702.512 kb on - strand, within DZA65_RS17140 at 3702.512 kb on - strand, within DZA65_RS17140 at 3702.512 kb on - strand, within DZA65_RS17140 at 3702.512 kb on - strand, within DZA65_RS17140 at 3702.512 kb on - strand, within DZA65_RS17140 at 3702.512 kb on - strand, within DZA65_RS17140 at 3702.552 kb on + strand, within DZA65_RS17140 at 3702.600 kb on + strand, within DZA65_RS17140 at 3702.600 kb on + strand, within DZA65_RS17140 at 3702.601 kb on - strand, within DZA65_RS17140 at 3702.601 kb on - strand, within DZA65_RS17140 at 3702.622 kb on + strand, within DZA65_RS17140 at 3702.622 kb on + strand, within DZA65_RS17140 at 3702.622 kb on + strand, within DZA65_RS17140 at 3702.622 kb on + strand, within DZA65_RS17140 at 3702.623 kb on - strand, within DZA65_RS17140 at 3702.623 kb on - strand, within DZA65_RS17140 at 3702.719 kb on - strand, within DZA65_RS17140 at 3702.719 kb on - strand, within DZA65_RS17140 at 3702.719 kb on - strand, within DZA65_RS17140 at 3702.721 kb on + strand, within DZA65_RS17140 at 3702.721 kb on + strand, within DZA65_RS17140 at 3702.721 kb on + strand, within DZA65_RS17140 at 3702.721 kb on + strand, within DZA65_RS17140 at 3702.721 kb on + strand, within DZA65_RS17140 at 3702.722 kb on - strand, within DZA65_RS17140 at 3702.722 kb on - strand, within DZA65_RS17140 at 3702.886 kb on - strand, within DZA65_RS17140 at 3702.886 kb on - strand, within DZA65_RS17140 at 3702.906 kb on + strand, within DZA65_RS17140 at 3702.906 kb on + strand at 3702.906 kb on + strand, within DZA65_RS17140 at 3702.906 kb on + strand at 3702.906 kb on + strand, within DZA65_RS17140 at 3702.907 kb on - strand, within DZA65_RS17140 at 3702.907 kb on - strand, within DZA65_RS17140 at 3702.909 kb on + strand, within DZA65_RS17140 at 3702.909 kb on + strand, within DZA65_RS17140 at 3702.909 kb on + strand, within DZA65_RS17140 at 3702.909 kb on + strand, within DZA65_RS17140 at 3702.910 kb on - strand, within DZA65_RS17140 at 3702.937 kb on + strand, within DZA65_RS17140 at 3702.938 kb on - strand, within DZA65_RS17140 at 3702.938 kb on - strand, within DZA65_RS17140 at 3702.986 kb on + strand, within DZA65_RS17140 at 3702.986 kb on + strand, within DZA65_RS17140 at 3702.986 kb on + strand, within DZA65_RS17140 at 3702.987 kb on - strand, within DZA65_RS17140 at 3702.987 kb on - strand, within DZA65_RS17140 at 3702.987 kb on - strand, within DZA65_RS17140 at 3703.034 kb on + strand, within DZA65_RS17140 at 3703.035 kb on - strand, within DZA65_RS17140 at 3703.035 kb on - strand, within DZA65_RS17140 at 3703.035 kb on - strand, within DZA65_RS17140 at 3703.036 kb on + strand, within DZA65_RS17140 at 3703.036 kb on + strand, within DZA65_RS17140 at 3703.037 kb on - strand, within DZA65_RS17140 at 3703.290 kb on + strand at 3703.290 kb on + strand at 3703.291 kb on - strand at 3703.291 kb on - strand at 3703.291 kb on - strand at 3703.304 kb on + strand at 3703.304 kb on + strand at 3703.305 kb on - strand at 3703.338 kb on + strand at 3703.338 kb on + strand at 3703.338 kb on + strand at 3703.339 kb on - strand at 3703.339 kb on - strand at 3703.339 kb on - strand at 3703.339 kb on - strand at 3703.339 kb on - strand at 3703.339 kb on - strand at 3703.339 kb on - strand at 3703.339 kb on - strand at 3703.339 kb on - strand at 3703.394 kb on + strand at 3703.394 kb on + strand at 3703.394 kb on + strand at 3703.395 kb on - strand at 3703.397 kb on + strand at 3703.397 kb on + strand at 3703.397 kb on + strand at 3703.397 kb on + strand at 3703.416 kb on - strand at 3703.433 kb on - strand at 3703.529 kb on + strand at 3703.529 kb on + strand at 3703.530 kb on - strand at 3703.530 kb on - strand at 3703.549 kb on + strand, within DZA65_RS17145 at 3703.550 kb on - strand, within DZA65_RS17145 at 3703.550 kb on - strand, within DZA65_RS17145 at 3703.550 kb on - strand, within DZA65_RS17145 at 3703.554 kb on + strand, within DZA65_RS17145 at 3703.584 kb on + strand, within DZA65_RS17145 at 3703.584 kb on + strand, within DZA65_RS17145 at 3703.832 kb on + strand at 3703.833 kb on - strand, within DZA65_RS17145 at 3703.911 kb on + strand, within DZA65_RS17145 at 3703.941 kb on + strand, within DZA65_RS17145 at 3704.190 kb on + strand, within DZA65_RS17145 at 3704.190 kb on + strand, within DZA65_RS17145 at 3704.208 kb on + strand, within DZA65_RS17145 at 3704.248 kb on - strand, within DZA65_RS17145 at 3704.424 kb on + strand, within DZA65_RS17145 at 3704.444 kb on - strand, within DZA65_RS17145 at 3704.501 kb on + strand, within DZA65_RS17145 at 3704.571 kb on - strand, within DZA65_RS17145 at 3704.650 kb on + strand, within DZA65_RS17145 at 3704.651 kb on - strand, within DZA65_RS17145 at 3704.651 kb on - strand, within DZA65_RS17145 at 3704.651 kb on - strand, within DZA65_RS17145 at 3704.810 kb on + strand at 3705.335 kb on - strand at 3705.362 kb on - strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.395 kb on + strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.396 kb on - strand at 3705.398 kb on + strand at 3705.398 kb on + strand at 3705.398 kb on + strand at 3705.398 kb on + strand at 3705.398 kb on + strand at 3705.398 kb on + strand at 3705.414 kb on + strand at 3705.446 kb on + strand at 3705.446 kb on + strand at 3705.447 kb on - strand at 3705.447 kb on - strand at 3705.447 kb on - strand at 3705.519 kb on + strand at 3705.519 kb on + strand at 3705.520 kb on - strand at 3705.546 kb on + strand, within DZA65_RS17155 at 3705.546 kb on + strand, within DZA65_RS17155 at 3705.547 kb on - strand, within DZA65_RS17155 at 3705.591 kb on - strand, within DZA65_RS17155 at 3705.598 kb on + strand, within DZA65_RS17155 at 3705.638 kb on + strand, within DZA65_RS17155 at 3705.639 kb on - strand, within DZA65_RS17155 at 3705.646 kb on - strand, within DZA65_RS17155 at 3705.661 kb on + strand, within DZA65_RS17155 at 3705.708 kb on - strand, within DZA65_RS17155 at 3705.773 kb on + strand, within DZA65_RS17155 at 3705.773 kb on + strand, within DZA65_RS17155 at 3705.774 kb on - strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.784 kb on + strand, within DZA65_RS17155 at 3705.785 kb on - strand, within DZA65_RS17155 at 3705.785 kb on - strand, within DZA65_RS17155 at 3705.785 kb on - strand, within DZA65_RS17155 at 3705.785 kb on - strand, within DZA65_RS17155
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,702,413 - +2.6 3,702,420 + +0.5 3,702,420 + -0.7 3,702,439 + -1.0 3,702,439 + +0.5 3,702,440 - +0.2 3,702,440 - -0.1 3,702,440 - -0.5 3,702,440 - -0.4 3,702,511 + -1.4 3,702,511 + +0.0 3,702,511 + -0.1 3,702,511 + -1.1 3,702,512 - DZA65_RS17140 0.10 +0.5 3,702,512 - DZA65_RS17140 0.10 +0.8 3,702,512 - DZA65_RS17140 0.10 +1.2 3,702,512 - DZA65_RS17140 0.10 -0.5 3,702,512 - DZA65_RS17140 0.10 +1.2 3,702,512 - DZA65_RS17140 0.10 +0.5 3,702,512 - DZA65_RS17140 0.10 +0.0 3,702,552 + DZA65_RS17140 0.14 +0.2 3,702,600 + DZA65_RS17140 0.20 -0.5 3,702,600 + DZA65_RS17140 0.20 +0.6 3,702,601 - DZA65_RS17140 0.20 +2.0 3,702,601 - DZA65_RS17140 0.20 +1.7 3,702,622 + DZA65_RS17140 0.22 -0.4 3,702,622 + DZA65_RS17140 0.22 -0.5 3,702,622 + DZA65_RS17140 0.22 +0.7 3,702,622 + DZA65_RS17140 0.22 +0.5 3,702,623 - DZA65_RS17140 0.22 +0.4 3,702,623 - DZA65_RS17140 0.22 -1.3 3,702,719 - DZA65_RS17140 0.33 +1.5 3,702,719 - DZA65_RS17140 0.33 -0.5 3,702,719 - DZA65_RS17140 0.33 -2.4 3,702,721 + DZA65_RS17140 0.33 +0.4 3,702,721 + DZA65_RS17140 0.33 +0.2 3,702,721 + DZA65_RS17140 0.33 +2.6 3,702,721 + DZA65_RS17140 0.33 -1.6 3,702,721 + DZA65_RS17140 0.33 -0.5 3,702,722 - DZA65_RS17140 0.33 -1.3 3,702,722 - DZA65_RS17140 0.33 -0.3 3,702,886 - DZA65_RS17140 0.51 +0.8 3,702,886 - DZA65_RS17140 0.51 +1.3 3,702,906 + DZA65_RS17140 0.53 +1.2 3,702,906 + +2.5 3,702,906 + DZA65_RS17140 0.53 +2.0 3,702,906 + +0.0 3,702,906 + DZA65_RS17140 0.53 -0.3 3,702,907 - DZA65_RS17140 0.53 -1.3 3,702,907 - DZA65_RS17140 0.53 +1.0 3,702,909 + DZA65_RS17140 0.54 -0.7 3,702,909 + DZA65_RS17140 0.54 +1.0 3,702,909 + DZA65_RS17140 0.54 -0.4 3,702,909 + DZA65_RS17140 0.54 +0.7 3,702,910 - DZA65_RS17140 0.54 -1.8 3,702,937 + DZA65_RS17140 0.57 -0.3 3,702,938 - DZA65_RS17140 0.57 +1.0 3,702,938 - DZA65_RS17140 0.57 -1.0 3,702,986 + DZA65_RS17140 0.62 -0.2 3,702,986 + DZA65_RS17140 0.62 -1.0 3,702,986 + DZA65_RS17140 0.62 -1.0 3,702,987 - DZA65_RS17140 0.62 +2.3 3,702,987 - DZA65_RS17140 0.62 +1.5 3,702,987 - DZA65_RS17140 0.62 +0.1 3,703,034 + DZA65_RS17140 0.67 +0.0 3,703,035 - DZA65_RS17140 0.68 -0.2 3,703,035 - DZA65_RS17140 0.68 -1.5 3,703,035 - DZA65_RS17140 0.68 -0.2 3,703,036 + DZA65_RS17140 0.68 -1.0 3,703,036 + DZA65_RS17140 0.68 -0.2 3,703,037 - DZA65_RS17140 0.68 +0.1 3,703,290 + +0.3 3,703,290 + +0.7 3,703,291 - -0.2 3,703,291 - -1.5 3,703,291 - -0.5 3,703,304 + +0.3 3,703,304 + -1.0 3,703,305 - +0.4 3,703,338 + -1.2 3,703,338 + -1.8 3,703,338 + +1.3 3,703,339 - -1.0 3,703,339 - +0.7 3,703,339 - +1.0 3,703,339 - -0.7 3,703,339 - +1.3 3,703,339 - +1.0 3,703,339 - +0.5 3,703,339 - -0.5 3,703,339 - +0.2 3,703,394 + +0.5 3,703,394 + +1.9 3,703,394 + +1.4 3,703,395 - +0.7 3,703,397 + -2.8 3,703,397 + -0.6 3,703,397 + +0.6 3,703,397 + -1.0 3,703,416 - -1.5 3,703,433 - +1.3 3,703,529 + +0.8 3,703,529 + -1.0 3,703,530 - -0.3 3,703,530 - -1.3 3,703,549 + DZA65_RS17145 0.11 -0.5 3,703,550 - DZA65_RS17145 0.11 -1.0 3,703,550 - DZA65_RS17145 0.11 -0.8 3,703,550 - DZA65_RS17145 0.11 +1.6 3,703,554 + DZA65_RS17145 0.11 +0.9 3,703,584 + DZA65_RS17145 0.13 -1.0 3,703,584 + DZA65_RS17145 0.13 +1.9 3,703,832 + -1.0 3,703,833 - DZA65_RS17145 0.31 +0.7 3,703,911 + DZA65_RS17145 0.37 +0.7 3,703,941 + DZA65_RS17145 0.39 -0.8 3,704,190 + DZA65_RS17145 0.56 +0.7 3,704,190 + DZA65_RS17145 0.56 -1.0 3,704,208 + DZA65_RS17145 0.58 +0.9 3,704,248 - DZA65_RS17145 0.61 +1.0 3,704,424 + DZA65_RS17145 0.73 -0.8 3,704,444 - DZA65_RS17145 0.75 +0.0 3,704,501 + DZA65_RS17145 0.79 +1.3 3,704,571 - DZA65_RS17145 0.84 -0.5 3,704,650 + DZA65_RS17145 0.89 +0.9 3,704,651 - DZA65_RS17145 0.89 +1.5 3,704,651 - DZA65_RS17145 0.89 -1.5 3,704,651 - DZA65_RS17145 0.89 -2.7 3,704,810 + -0.3 3,705,335 - +0.7 3,705,362 - -0.2 3,705,395 + +0.2 3,705,395 + -0.5 3,705,395 + +0.6 3,705,395 + -0.2 3,705,395 + -0.2 3,705,395 + +0.7 3,705,395 + -1.8 3,705,395 + -0.2 3,705,395 + +0.2 3,705,395 + -1.0 3,705,395 + -0.5 3,705,395 + -2.0 3,705,396 - +0.0 3,705,396 - -1.9 3,705,396 - -1.4 3,705,396 - -1.1 3,705,396 - -0.8 3,705,396 - -2.0 3,705,396 - -1.0 3,705,396 - -2.3 3,705,398 + -1.1 3,705,398 + +1.1 3,705,398 + -0.7 3,705,398 + -1.3 3,705,398 + -0.5 3,705,398 + +1.6 3,705,414 + -1.5 3,705,446 + -1.7 3,705,446 + -2.0 3,705,447 - -1.1 3,705,447 - -1.3 3,705,447 - -3.0 3,705,519 + +0.0 3,705,519 + +0.0 3,705,520 - -0.8 3,705,546 + DZA65_RS17155 0.12 -1.3 3,705,546 + DZA65_RS17155 0.12 -1.3 3,705,547 - DZA65_RS17155 0.13 +0.5 3,705,591 - DZA65_RS17155 0.18 -2.3 3,705,598 + DZA65_RS17155 0.19 -1.3 3,705,638 + DZA65_RS17155 0.24 -1.4 3,705,639 - DZA65_RS17155 0.24 -2.8 3,705,646 - DZA65_RS17155 0.25 -1.5 3,705,661 + DZA65_RS17155 0.27 -0.3 3,705,708 - DZA65_RS17155 0.33 -2.1 3,705,773 + DZA65_RS17155 0.41 -3.9 3,705,773 + DZA65_RS17155 0.41 -0.1 3,705,774 - DZA65_RS17155 0.41 -2.4 3,705,784 + DZA65_RS17155 0.42 -1.5 3,705,784 + DZA65_RS17155 0.42 -1.3 3,705,784 + DZA65_RS17155 0.42 -0.5 3,705,784 + DZA65_RS17155 0.42 -3.1 3,705,784 + DZA65_RS17155 0.42 -3.1 3,705,784 + DZA65_RS17155 0.42 -1.3 3,705,785 - DZA65_RS17155 0.43 -1.5 3,705,785 - DZA65_RS17155 0.43 -3.3 3,705,785 - DZA65_RS17155 0.43 -1.5 3,705,785 - DZA65_RS17155 0.43 -1.3
Or see this region's nucleotide sequence