Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS16365 and DZA65_RS16370 are separated by 178 nucleotides DZA65_RS16370 and DZA65_RS16375 are separated by 33 nucleotides DZA65_RS16375 and DZA65_RS16380 are separated by 70 nucleotides DZA65_RS16380 and DZA65_RS22950 are separated by 9 nucleotides DZA65_RS22950 and DZA65_RS16385 are separated by 1 nucleotides
DZA65_RS16365: DZA65_RS16365 - hypothetical protein, at 3,547,010 to 3,547,270
_RS16365
DZA65_RS16370: DZA65_RS16370 - hypothetical protein, at 3,547,449 to 3,548,081
_RS16370
DZA65_RS16375: DZA65_RS16375 - hypothetical protein, at 3,548,115 to 3,548,426
_RS16375
DZA65_RS16380: DZA65_RS16380 - hypothetical protein, at 3,548,497 to 3,548,820
_RS16380
DZA65_RS22950: DZA65_RS22950 - hypothetical protein, at 3,548,830 to 3,548,985
_RS22950
DZA65_RS16385: DZA65_RS16385 - hypothetical protein, at 3,548,987 to 3,551,533
_RS16385
Position (kb)
3547
3548
3549 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3546.487 kb on + strand at 3546.487 kb on + strand at 3546.488 kb on - strand at 3546.505 kb on - strand at 3546.552 kb on + strand at 3546.553 kb on - strand at 3546.553 kb on - strand at 3546.578 kb on - strand at 3546.591 kb on + strand at 3546.592 kb on - strand at 3546.647 kb on + strand at 3546.648 kb on - strand at 3546.648 kb on - strand at 3546.648 kb on - strand at 3546.659 kb on + strand at 3546.659 kb on + strand at 3546.660 kb on - strand at 3546.759 kb on + strand at 3546.759 kb on + strand at 3546.759 kb on + strand at 3546.760 kb on - strand at 3546.760 kb on - strand at 3546.792 kb on + strand at 3546.794 kb on + strand at 3546.794 kb on + strand at 3546.794 kb on + strand at 3546.794 kb on + strand at 3546.794 kb on + strand at 3546.795 kb on - strand at 3546.795 kb on - strand at 3546.795 kb on - strand at 3546.795 kb on - strand at 3546.795 kb on - strand at 3546.828 kb on + strand at 3546.829 kb on - strand at 3546.850 kb on - strand at 3546.861 kb on - strand at 3546.957 kb on - strand at 3547.005 kb on + strand at 3547.005 kb on + strand at 3547.005 kb on + strand at 3547.006 kb on - strand at 3547.006 kb on - strand at 3547.006 kb on - strand at 3547.006 kb on - strand at 3547.010 kb on - strand at 3547.023 kb on - strand at 3547.023 kb on - strand at 3547.089 kb on + strand, within DZA65_RS16365 at 3547.090 kb on - strand, within DZA65_RS16365 at 3547.090 kb on - strand, within DZA65_RS16365 at 3547.090 kb on - strand, within DZA65_RS16365 at 3547.184 kb on + strand, within DZA65_RS16365 at 3547.184 kb on + strand, within DZA65_RS16365 at 3547.184 kb on + strand, within DZA65_RS16365 at 3547.184 kb on + strand, within DZA65_RS16365 at 3547.184 kb on + strand, within DZA65_RS16365 at 3547.185 kb on - strand, within DZA65_RS16365 at 3547.185 kb on - strand, within DZA65_RS16365 at 3547.185 kb on - strand, within DZA65_RS16365 at 3547.185 kb on - strand, within DZA65_RS16365 at 3547.185 kb on - strand, within DZA65_RS16365 at 3547.185 kb on - strand, within DZA65_RS16365 at 3547.185 kb on - strand, within DZA65_RS16365 at 3547.204 kb on - strand, within DZA65_RS16365 at 3547.235 kb on + strand, within DZA65_RS16365 at 3547.235 kb on + strand, within DZA65_RS16365 at 3547.235 kb on + strand, within DZA65_RS16365 at 3547.252 kb on + strand at 3547.252 kb on + strand at 3547.253 kb on - strand at 3547.268 kb on + strand at 3547.268 kb on + strand at 3547.268 kb on + strand at 3547.268 kb on + strand at 3547.280 kb on - strand at 3547.331 kb on + strand at 3547.331 kb on + strand at 3547.332 kb on - strand at 3547.332 kb on - strand at 3547.376 kb on + strand at 3547.376 kb on + strand at 3547.376 kb on + strand at 3547.376 kb on + strand at 3547.377 kb on - strand at 3547.389 kb on + strand at 3547.402 kb on + strand at 3547.403 kb on - strand at 3547.403 kb on - strand at 3547.404 kb on + strand at 3547.404 kb on + strand at 3547.404 kb on + strand at 3547.404 kb on + strand at 3547.404 kb on + strand at 3547.409 kb on + strand at 3547.409 kb on + strand at 3547.409 kb on + strand at 3547.409 kb on + strand at 3547.410 kb on - strand at 3547.419 kb on + strand at 3547.419 kb on + strand at 3547.420 kb on - strand at 3547.432 kb on + strand at 3547.432 kb on + strand at 3547.432 kb on + strand at 3547.432 kb on + strand at 3547.432 kb on + strand at 3547.432 kb on + strand at 3547.432 kb on + strand at 3547.432 kb on + strand at 3547.433 kb on - strand at 3547.433 kb on - strand at 3547.530 kb on + strand, within DZA65_RS16370 at 3547.530 kb on + strand, within DZA65_RS16370 at 3547.530 kb on + strand, within DZA65_RS16370 at 3547.570 kb on + strand, within DZA65_RS16370 at 3548.060 kb on + strand at 3548.061 kb on - strand at 3548.071 kb on + strand at 3548.071 kb on + strand at 3548.071 kb on + strand at 3548.071 kb on + strand at 3548.071 kb on + strand at 3548.071 kb on + strand at 3548.072 kb on - strand at 3548.072 kb on - strand at 3548.072 kb on - strand at 3548.075 kb on + strand at 3548.075 kb on + strand at 3548.085 kb on + strand at 3548.086 kb on - strand at 3548.104 kb on + strand at 3548.160 kb on + strand, within DZA65_RS16375 at 3548.161 kb on - strand, within DZA65_RS16375 at 3548.161 kb on - strand, within DZA65_RS16375 at 3548.165 kb on + strand, within DZA65_RS16375 at 3548.165 kb on + strand, within DZA65_RS16375 at 3548.166 kb on - strand, within DZA65_RS16375 at 3548.172 kb on - strand, within DZA65_RS16375 at 3548.172 kb on - strand, within DZA65_RS16375 at 3548.172 kb on - strand, within DZA65_RS16375 at 3548.183 kb on + strand, within DZA65_RS16375 at 3548.189 kb on + strand, within DZA65_RS16375 at 3548.196 kb on + strand, within DZA65_RS16375 at 3548.197 kb on - strand, within DZA65_RS16375 at 3548.212 kb on - strand, within DZA65_RS16375 at 3548.273 kb on + strand, within DZA65_RS16375 at 3548.274 kb on - strand, within DZA65_RS16375 at 3548.293 kb on + strand, within DZA65_RS16375 at 3548.293 kb on + strand, within DZA65_RS16375 at 3548.293 kb on + strand, within DZA65_RS16375 at 3548.293 kb on + strand, within DZA65_RS16375 at 3548.293 kb on + strand, within DZA65_RS16375 at 3548.325 kb on - strand, within DZA65_RS16375 at 3548.325 kb on - strand, within DZA65_RS16375 at 3548.325 kb on - strand, within DZA65_RS16375 at 3548.373 kb on + strand, within DZA65_RS16375 at 3548.426 kb on + strand at 3548.453 kb on + strand at 3548.453 kb on + strand at 3548.454 kb on - strand at 3548.454 kb on - strand at 3548.454 kb on - strand at 3548.456 kb on - strand at 3548.458 kb on + strand at 3548.467 kb on + strand at 3548.509 kb on - strand at 3548.541 kb on + strand, within DZA65_RS16380 at 3548.620 kb on + strand, within DZA65_RS16380 at 3548.620 kb on + strand, within DZA65_RS16380 at 3548.620 kb on + strand, within DZA65_RS16380 at 3548.621 kb on - strand, within DZA65_RS16380 at 3548.653 kb on + strand, within DZA65_RS16380 at 3548.653 kb on + strand, within DZA65_RS16380 at 3548.653 kb on + strand, within DZA65_RS16380 at 3548.654 kb on - strand, within DZA65_RS16380 at 3548.654 kb on - strand, within DZA65_RS16380 at 3548.684 kb on - strand, within DZA65_RS16380 at 3548.696 kb on - strand, within DZA65_RS16380 at 3548.753 kb on + strand, within DZA65_RS16380 at 3548.760 kb on + strand, within DZA65_RS16380 at 3548.761 kb on - strand, within DZA65_RS16380 at 3548.761 kb on - strand, within DZA65_RS16380 at 3548.761 kb on - strand, within DZA65_RS16380 at 3548.761 kb on - strand, within DZA65_RS16380 at 3548.761 kb on - strand, within DZA65_RS16380 at 3548.761 kb on - strand, within DZA65_RS16380 at 3548.783 kb on - strand, within DZA65_RS16380 at 3548.786 kb on - strand, within DZA65_RS16380 at 3548.821 kb on - strand at 3548.832 kb on - strand at 3548.850 kb on + strand, within DZA65_RS22950 at 3548.853 kb on - strand, within DZA65_RS22950 at 3548.853 kb on - strand, within DZA65_RS22950 at 3548.887 kb on + strand, within DZA65_RS22950 at 3548.887 kb on + strand, within DZA65_RS22950 at 3548.930 kb on - strand, within DZA65_RS22950 at 3548.950 kb on + strand, within DZA65_RS22950 at 3548.952 kb on + strand, within DZA65_RS22950 at 3548.953 kb on - strand, within DZA65_RS22950 at 3548.953 kb on - strand, within DZA65_RS22950 at 3548.953 kb on - strand, within DZA65_RS22950 at 3548.956 kb on + strand, within DZA65_RS22950 at 3548.957 kb on - strand, within DZA65_RS22950 at 3548.957 kb on - strand, within DZA65_RS22950 at 3548.957 kb on - strand, within DZA65_RS22950 at 3548.966 kb on + strand, within DZA65_RS22950 at 3548.967 kb on - strand, within DZA65_RS22950 at 3548.988 kb on + strand at 3548.988 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,546,487 + +0.5 3,546,487 + -1.2 3,546,488 - -0.0 3,546,505 - +0.6 3,546,552 + -0.1 3,546,553 - -0.4 3,546,553 - -1.8 3,546,578 - -0.4 3,546,591 + -0.1 3,546,592 - -0.7 3,546,647 + -0.0 3,546,648 - -0.0 3,546,648 - +0.8 3,546,648 - -0.9 3,546,659 + -1.0 3,546,659 + -1.2 3,546,660 - -0.8 3,546,759 + -1.4 3,546,759 + -0.0 3,546,759 + -1.0 3,546,760 - +0.0 3,546,760 - -1.8 3,546,792 + -1.1 3,546,794 + +0.1 3,546,794 + -1.2 3,546,794 + +0.9 3,546,794 + -1.0 3,546,794 + -0.9 3,546,795 - +0.6 3,546,795 - +1.0 3,546,795 - -0.0 3,546,795 - +0.8 3,546,795 - -0.0 3,546,828 + +1.1 3,546,829 - +1.0 3,546,850 - +1.0 3,546,861 - +1.0 3,546,957 - -0.6 3,547,005 + -0.9 3,547,005 + -0.9 3,547,005 + -0.9 3,547,006 - -0.3 3,547,006 - +0.7 3,547,006 - -1.4 3,547,006 - -0.1 3,547,010 - -1.4 3,547,023 - +0.6 3,547,023 - +1.0 3,547,089 + DZA65_RS16365 0.30 +0.9 3,547,090 - DZA65_RS16365 0.31 +0.6 3,547,090 - DZA65_RS16365 0.31 +0.3 3,547,090 - DZA65_RS16365 0.31 +0.4 3,547,184 + DZA65_RS16365 0.67 +0.2 3,547,184 + DZA65_RS16365 0.67 +2.1 3,547,184 + DZA65_RS16365 0.67 +0.4 3,547,184 + DZA65_RS16365 0.67 -0.8 3,547,184 + DZA65_RS16365 0.67 -0.0 3,547,185 - DZA65_RS16365 0.67 +0.2 3,547,185 - DZA65_RS16365 0.67 +0.4 3,547,185 - DZA65_RS16365 0.67 +0.2 3,547,185 - DZA65_RS16365 0.67 -0.5 3,547,185 - DZA65_RS16365 0.67 -1.0 3,547,185 - DZA65_RS16365 0.67 -0.3 3,547,185 - DZA65_RS16365 0.67 -0.2 3,547,204 - DZA65_RS16365 0.74 +0.3 3,547,235 + DZA65_RS16365 0.86 -0.6 3,547,235 + DZA65_RS16365 0.86 +0.7 3,547,235 + DZA65_RS16365 0.86 -0.0 3,547,252 + -0.1 3,547,252 + -0.2 3,547,253 - -0.6 3,547,268 + -1.6 3,547,268 + +0.3 3,547,268 + +0.1 3,547,268 + -0.5 3,547,280 - -0.4 3,547,331 + -0.6 3,547,331 + +0.9 3,547,332 - +0.4 3,547,332 - -0.2 3,547,376 + -1.0 3,547,376 + +0.6 3,547,376 + +0.4 3,547,376 + -0.3 3,547,377 - -1.0 3,547,389 + +1.0 3,547,402 + -1.1 3,547,403 - -0.9 3,547,403 - -1.6 3,547,404 + -1.9 3,547,404 + +1.2 3,547,404 + +0.2 3,547,404 + +1.0 3,547,404 + -1.4 3,547,409 + -1.4 3,547,409 + -1.8 3,547,409 + -0.4 3,547,409 + +1.9 3,547,410 - -0.0 3,547,419 + -0.0 3,547,419 + +1.3 3,547,420 - -2.0 3,547,432 + +2.0 3,547,432 + -2.0 3,547,432 + +0.2 3,547,432 + -1.8 3,547,432 + +0.2 3,547,432 + -0.0 3,547,432 + -2.0 3,547,432 + -0.2 3,547,433 - -1.8 3,547,433 - -1.6 3,547,530 + DZA65_RS16370 0.13 -0.4 3,547,530 + DZA65_RS16370 0.13 -0.0 3,547,530 + DZA65_RS16370 0.13 -1.3 3,547,570 + DZA65_RS16370 0.19 -0.2 3,548,060 + +2.6 3,548,061 - -0.4 3,548,071 + +0.1 3,548,071 + +1.6 3,548,071 + -0.6 3,548,071 + +0.2 3,548,071 + +0.0 3,548,071 + -0.0 3,548,072 - +2.6 3,548,072 - -0.5 3,548,072 - +2.0 3,548,075 + -1.8 3,548,075 + +0.7 3,548,085 + -2.9 3,548,086 - -0.4 3,548,104 + +1.1 3,548,160 + DZA65_RS16375 0.14 +0.4 3,548,161 - DZA65_RS16375 0.15 +1.5 3,548,161 - DZA65_RS16375 0.15 -0.3 3,548,165 + DZA65_RS16375 0.16 +0.8 3,548,165 + DZA65_RS16375 0.16 -0.6 3,548,166 - DZA65_RS16375 0.16 +0.6 3,548,172 - DZA65_RS16375 0.18 +1.0 3,548,172 - DZA65_RS16375 0.18 +0.4 3,548,172 - DZA65_RS16375 0.18 -1.1 3,548,183 + DZA65_RS16375 0.22 -1.5 3,548,189 + DZA65_RS16375 0.24 +0.3 3,548,196 + DZA65_RS16375 0.26 -0.8 3,548,197 - DZA65_RS16375 0.26 +1.0 3,548,212 - DZA65_RS16375 0.31 -1.0 3,548,273 + DZA65_RS16375 0.51 +2.4 3,548,274 - DZA65_RS16375 0.51 +0.6 3,548,293 + DZA65_RS16375 0.57 +1.5 3,548,293 + DZA65_RS16375 0.57 -0.8 3,548,293 + DZA65_RS16375 0.57 -0.3 3,548,293 + DZA65_RS16375 0.57 -0.5 3,548,293 + DZA65_RS16375 0.57 -1.4 3,548,325 - DZA65_RS16375 0.67 +0.3 3,548,325 - DZA65_RS16375 0.67 -0.4 3,548,325 - DZA65_RS16375 0.67 -1.0 3,548,373 + DZA65_RS16375 0.83 -0.2 3,548,426 + +0.4 3,548,453 + -1.4 3,548,453 + -0.8 3,548,454 - -0.1 3,548,454 - -0.5 3,548,454 - +0.7 3,548,456 - -0.0 3,548,458 + +0.1 3,548,467 + -0.8 3,548,509 - -0.0 3,548,541 + DZA65_RS16380 0.14 -1.7 3,548,620 + DZA65_RS16380 0.38 -0.6 3,548,620 + DZA65_RS16380 0.38 +0.2 3,548,620 + DZA65_RS16380 0.38 +0.4 3,548,621 - DZA65_RS16380 0.38 -0.4 3,548,653 + DZA65_RS16380 0.48 -2.0 3,548,653 + DZA65_RS16380 0.48 -1.2 3,548,653 + DZA65_RS16380 0.48 +0.6 3,548,654 - DZA65_RS16380 0.48 -0.9 3,548,654 - DZA65_RS16380 0.48 -0.8 3,548,684 - DZA65_RS16380 0.58 -0.8 3,548,696 - DZA65_RS16380 0.61 -1.6 3,548,753 + DZA65_RS16380 0.79 +0.5 3,548,760 + DZA65_RS16380 0.81 +0.2 3,548,761 - DZA65_RS16380 0.81 -1.6 3,548,761 - DZA65_RS16380 0.81 -1.2 3,548,761 - DZA65_RS16380 0.81 -0.2 3,548,761 - DZA65_RS16380 0.81 -0.0 3,548,761 - DZA65_RS16380 0.81 -0.6 3,548,761 - DZA65_RS16380 0.81 -1.5 3,548,783 - DZA65_RS16380 0.88 -0.2 3,548,786 - DZA65_RS16380 0.89 +1.2 3,548,821 - +0.1 3,548,832 - +0.1 3,548,850 + DZA65_RS22950 0.13 -0.3 3,548,853 - DZA65_RS22950 0.15 -1.4 3,548,853 - DZA65_RS22950 0.15 +0.4 3,548,887 + DZA65_RS22950 0.37 -0.2 3,548,887 + DZA65_RS22950 0.37 +1.6 3,548,930 - DZA65_RS22950 0.64 -0.2 3,548,950 + DZA65_RS22950 0.77 +0.2 3,548,952 + DZA65_RS22950 0.78 -0.4 3,548,953 - DZA65_RS22950 0.79 +0.4 3,548,953 - DZA65_RS22950 0.79 -1.4 3,548,953 - DZA65_RS22950 0.79 +0.3 3,548,956 + DZA65_RS22950 0.81 -1.4 3,548,957 - DZA65_RS22950 0.81 +0.6 3,548,957 - DZA65_RS22950 0.81 -0.3 3,548,957 - DZA65_RS22950 0.81 +1.3 3,548,966 + DZA65_RS22950 0.87 +0.1 3,548,967 - DZA65_RS22950 0.88 +2.2 3,548,988 + +1.6 3,548,988 + -0.4
Or see this region's nucleotide sequence