Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS04220 and DZA65_RS04225 overlap by 8 nucleotides DZA65_RS04225 and DZA65_RS04230 are separated by 58 nucleotides DZA65_RS04230 and DZA65_RS04235 are separated by 111 nucleotides DZA65_RS04235 and DZA65_RS04240 are separated by 83 nucleotides
DZA65_RS04220: DZA65_RS04220 - type II toxin-antitoxin system RatA family toxin, at 881,891 to 882,325
_RS04220
DZA65_RS04225: DZA65_RS04225 - RnfH family protein, at 882,318 to 882,611
_RS04225
DZA65_RS04230: DZA65_RS04230 - outer membrane protein assembly factor BamE, at 882,670 to 883,014
_RS04230
DZA65_RS04235: DZA65_RS04235 - DNA repair protein RecN, at 883,126 to 884,787
_RS04235
DZA65_RS04240: DZA65_RS04240 - NAD(+) kinase, at 884,871 to 885,749
_RS04240
Position (kb) 883
884
885 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 882.167 kb on - strand, within DZA65_RS04220 at 882.190 kb on + strand, within DZA65_RS04220 at 882.190 kb on + strand, within DZA65_RS04220 at 882.190 kb on + strand, within DZA65_RS04220 at 882.190 kb on + strand, within DZA65_RS04220 at 882.191 kb on - strand, within DZA65_RS04220 at 882.191 kb on - strand, within DZA65_RS04220 at 882.191 kb on - strand, within DZA65_RS04220 at 882.277 kb on + strand, within DZA65_RS04220 at 882.277 kb on + strand, within DZA65_RS04220 at 882.314 kb on + strand at 882.314 kb on + strand at 882.314 kb on + strand at 882.315 kb on - strand at 882.315 kb on - strand at 882.346 kb on - strand at 882.366 kb on + strand at 882.367 kb on - strand, within DZA65_RS04225 at 882.414 kb on - strand, within DZA65_RS04225 at 882.508 kb on + strand, within DZA65_RS04225 at 882.508 kb on + strand, within DZA65_RS04225 at 882.508 kb on + strand, within DZA65_RS04225 at 882.508 kb on + strand, within DZA65_RS04225 at 882.508 kb on + strand, within DZA65_RS04225 at 882.509 kb on - strand, within DZA65_RS04225 at 882.509 kb on - strand, within DZA65_RS04225 at 882.509 kb on - strand, within DZA65_RS04225 at 882.560 kb on + strand, within DZA65_RS04225 at 882.568 kb on + strand, within DZA65_RS04225 at 882.569 kb on - strand, within DZA65_RS04225 at 882.671 kb on + strand at 882.671 kb on + strand at 882.671 kb on + strand at 882.671 kb on + strand at 882.672 kb on - strand at 882.672 kb on - strand at 882.672 kb on - strand at 882.672 kb on - strand at 882.672 kb on - strand at 882.672 kb on - strand at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.789 kb on - strand, within DZA65_RS04230 at 882.789 kb on - strand at 882.842 kb on + strand, within DZA65_RS04230 at 882.842 kb on + strand, within DZA65_RS04230 at 882.842 kb on + strand, within DZA65_RS04230 at 882.842 kb on + strand, within DZA65_RS04230 at 882.843 kb on - strand, within DZA65_RS04230 at 882.843 kb on - strand, within DZA65_RS04230 at 882.843 kb on - strand, within DZA65_RS04230 at 882.843 kb on - strand, within DZA65_RS04230 at 882.843 kb on - strand, within DZA65_RS04230 at 882.883 kb on + strand, within DZA65_RS04230 at 882.884 kb on - strand, within DZA65_RS04230 at 882.902 kb on + strand, within DZA65_RS04230 at 882.902 kb on + strand, within DZA65_RS04230 at 882.903 kb on - strand, within DZA65_RS04230 at 882.903 kb on - strand, within DZA65_RS04230 at 882.903 kb on - strand, within DZA65_RS04230 at 882.903 kb on - strand, within DZA65_RS04230 at 882.903 kb on - strand, within DZA65_RS04230 at 882.934 kb on + strand, within DZA65_RS04230 at 882.934 kb on + strand, within DZA65_RS04230 at 882.934 kb on + strand, within DZA65_RS04230 at 882.935 kb on - strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.970 kb on - strand, within DZA65_RS04230 at 882.970 kb on - strand, within DZA65_RS04230 at 882.970 kb on - strand, within DZA65_RS04230 at 883.005 kb on + strand at 883.014 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.038 kb on + strand at 883.038 kb on + strand at 883.038 kb on + strand at 883.039 kb on - strand at 883.052 kb on + strand at 883.052 kb on + strand at 883.052 kb on + strand at 883.052 kb on + strand at 883.053 kb on - strand at 883.053 kb on - strand at 883.053 kb on - strand at 883.055 kb on + strand at 883.055 kb on + strand at 883.055 kb on + strand at 883.055 kb on + strand at 883.055 kb on + strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.093 kb on - strand at 883.127 kb on + strand at 883.128 kb on - strand at 883.269 kb on + strand at 883.270 kb on - strand at 883.280 kb on + strand at 883.280 kb on + strand at 883.280 kb on + strand at 883.280 kb on + strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.339 kb on - strand, within DZA65_RS04235 at 883.339 kb on - strand, within DZA65_RS04235 at 883.461 kb on + strand, within DZA65_RS04235 at 883.498 kb on + strand, within DZA65_RS04235 at 883.498 kb on + strand, within DZA65_RS04235 at 883.498 kb on + strand, within DZA65_RS04235 at 883.498 kb on + strand, within DZA65_RS04235 at 883.499 kb on - strand, within DZA65_RS04235 at 883.536 kb on + strand, within DZA65_RS04235 at 883.536 kb on + strand, within DZA65_RS04235 at 883.536 kb on + strand, within DZA65_RS04235 at 883.537 kb on - strand, within DZA65_RS04235 at 883.783 kb on + strand, within DZA65_RS04235 at 883.783 kb on + strand, within DZA65_RS04235 at 883.783 kb on + strand, within DZA65_RS04235 at 883.784 kb on - strand, within DZA65_RS04235 at 883.784 kb on - strand, within DZA65_RS04235 at 883.784 kb on - strand, within DZA65_RS04235 at 883.784 kb on - strand, within DZA65_RS04235 at 883.784 kb on - strand, within DZA65_RS04235 at 883.784 kb on - strand, within DZA65_RS04235 at 883.784 kb on - strand, within DZA65_RS04235 at 883.801 kb on + strand, within DZA65_RS04235 at 883.801 kb on + strand, within DZA65_RS04235 at 883.801 kb on + strand, within DZA65_RS04235 at 883.801 kb on + strand, within DZA65_RS04235 at 883.801 kb on + strand, within DZA65_RS04235 at 883.801 kb on + strand, within DZA65_RS04235 at 883.801 kb on + strand, within DZA65_RS04235 at 883.802 kb on - strand, within DZA65_RS04235 at 883.802 kb on - strand, within DZA65_RS04235 at 883.802 kb on - strand, within DZA65_RS04235 at 883.802 kb on - strand, within DZA65_RS04235 at 883.802 kb on - strand, within DZA65_RS04235 at 883.811 kb on + strand, within DZA65_RS04235 at 883.812 kb on - strand, within DZA65_RS04235 at 883.892 kb on + strand, within DZA65_RS04235 at 883.892 kb on + strand, within DZA65_RS04235 at 883.893 kb on - strand, within DZA65_RS04235 at 883.893 kb on - strand, within DZA65_RS04235 at 883.893 kb on - strand, within DZA65_RS04235 at 883.893 kb on - strand, within DZA65_RS04235 at 883.893 kb on - strand, within DZA65_RS04235 at 883.917 kb on + strand, within DZA65_RS04235 at 883.918 kb on - strand, within DZA65_RS04235 at 883.918 kb on - strand, within DZA65_RS04235 at 883.928 kb on + strand, within DZA65_RS04235 at 884.147 kb on + strand, within DZA65_RS04235 at 884.147 kb on + strand, within DZA65_RS04235 at 884.147 kb on + strand, within DZA65_RS04235 at 884.147 kb on + strand, within DZA65_RS04235 at 884.147 kb on + strand, within DZA65_RS04235 at 884.148 kb on - strand, within DZA65_RS04235 at 884.148 kb on - strand, within DZA65_RS04235 at 884.148 kb on - strand, within DZA65_RS04235 at 884.148 kb on - strand, within DZA65_RS04235 at 884.148 kb on - strand, within DZA65_RS04235 at 884.148 kb on - strand, within DZA65_RS04235 at 884.148 kb on - strand, within DZA65_RS04235 at 884.169 kb on - strand, within DZA65_RS04235 at 884.214 kb on - strand, within DZA65_RS04235 at 884.323 kb on + strand, within DZA65_RS04235 at 884.323 kb on + strand, within DZA65_RS04235 at 884.323 kb on + strand, within DZA65_RS04235 at 884.323 kb on + strand, within DZA65_RS04235 at 884.324 kb on - strand, within DZA65_RS04235 at 884.324 kb on - strand, within DZA65_RS04235 at 884.324 kb on - strand, within DZA65_RS04235 at 884.324 kb on - strand, within DZA65_RS04235 at 884.324 kb on - strand at 884.324 kb on - strand, within DZA65_RS04235 at 884.324 kb on - strand, within DZA65_RS04235 at 884.377 kb on + strand, within DZA65_RS04235 at 884.377 kb on + strand, within DZA65_RS04235 at 884.378 kb on - strand, within DZA65_RS04235 at 884.378 kb on - strand, within DZA65_RS04235 at 884.378 kb on - strand, within DZA65_RS04235 at 884.571 kb on + strand, within DZA65_RS04235 at 884.571 kb on + strand, within DZA65_RS04235 at 884.572 kb on - strand, within DZA65_RS04235 at 884.572 kb on - strand, within DZA65_RS04235 at 884.673 kb on + strand at 884.673 kb on + strand at 884.673 kb on + strand at 884.673 kb on + strand at 884.674 kb on - strand at 884.674 kb on - strand at 884.674 kb on - strand at 884.763 kb on + strand at 884.764 kb on - strand at 884.790 kb on + strand at 884.790 kb on + strand at 884.791 kb on - strand at 884.800 kb on + strand at 884.800 kb on + strand at 884.801 kb on - strand at 884.801 kb on - strand at 884.811 kb on - strand at 884.811 kb on - strand at 884.820 kb on + strand at 884.821 kb on - strand at 884.824 kb on + strand at 884.825 kb on - strand at 884.829 kb on + strand at 884.831 kb on + strand at 884.847 kb on - strand at 884.866 kb on + strand at 884.866 kb on + strand at 885.761 kb on - strand at 885.761 kb on - strand at 885.761 kb on - strand at 885.761 kb on - strand at 885.761 kb on - strand at 885.761 kb on - strand at 885.761 kb on - strand at 885.761 kb on - strand at 885.761 kb on - strand at 885.761 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 882,167 - DZA65_RS04220 0.63 +0.2 882,190 + DZA65_RS04220 0.69 +0.8 882,190 + DZA65_RS04220 0.69 -2.6 882,190 + DZA65_RS04220 0.69 -0.5 882,190 + DZA65_RS04220 0.69 -1.2 882,191 - DZA65_RS04220 0.69 -1.1 882,191 - DZA65_RS04220 0.69 -0.1 882,191 - DZA65_RS04220 0.69 -1.2 882,277 + DZA65_RS04220 0.89 -0.3 882,277 + DZA65_RS04220 0.89 -0.1 882,314 + +0.1 882,314 + +0.5 882,314 + -0.9 882,315 - +1.7 882,315 - -0.6 882,346 - +1.1 882,366 + -0.9 882,367 - DZA65_RS04225 0.17 -0.1 882,414 - DZA65_RS04225 0.33 -0.1 882,508 + DZA65_RS04225 0.65 -0.7 882,508 + DZA65_RS04225 0.65 -0.4 882,508 + DZA65_RS04225 0.65 +0.9 882,508 + DZA65_RS04225 0.65 -2.1 882,508 + DZA65_RS04225 0.65 -0.4 882,509 - DZA65_RS04225 0.65 +0.2 882,509 - DZA65_RS04225 0.65 -1.5 882,509 - DZA65_RS04225 0.65 -1.2 882,560 + DZA65_RS04225 0.82 +0.4 882,568 + DZA65_RS04225 0.85 -1.4 882,569 - DZA65_RS04225 0.85 -0.5 882,671 + -1.0 882,671 + -0.3 882,671 + -0.9 882,671 + +0.1 882,672 - -1.5 882,672 - +0.9 882,672 - -0.9 882,672 - +0.6 882,672 - -0.1 882,672 - +0.2 882,788 + DZA65_RS04230 0.34 +0.2 882,788 + DZA65_RS04230 0.34 +1.1 882,788 + DZA65_RS04230 0.34 +0.1 882,788 + DZA65_RS04230 0.34 -0.7 882,788 + DZA65_RS04230 0.34 +1.8 882,788 + DZA65_RS04230 0.34 -1.5 882,788 + DZA65_RS04230 0.34 -0.1 882,789 - DZA65_RS04230 0.34 -1.3 882,789 - +2.7 882,842 + DZA65_RS04230 0.50 +1.2 882,842 + DZA65_RS04230 0.50 +0.8 882,842 + DZA65_RS04230 0.50 -0.1 882,842 + DZA65_RS04230 0.50 -0.7 882,843 - DZA65_RS04230 0.50 -1.4 882,843 - DZA65_RS04230 0.50 +0.9 882,843 - DZA65_RS04230 0.50 +0.5 882,843 - DZA65_RS04230 0.50 -2.3 882,843 - DZA65_RS04230 0.50 +0.8 882,883 + DZA65_RS04230 0.62 -0.9 882,884 - DZA65_RS04230 0.62 +1.9 882,902 + DZA65_RS04230 0.67 -0.2 882,902 + DZA65_RS04230 0.67 +0.4 882,903 - DZA65_RS04230 0.68 -0.9 882,903 - DZA65_RS04230 0.68 -0.3 882,903 - DZA65_RS04230 0.68 +1.1 882,903 - DZA65_RS04230 0.68 -1.3 882,903 - DZA65_RS04230 0.68 +0.6 882,934 + DZA65_RS04230 0.77 -0.3 882,934 + DZA65_RS04230 0.77 +1.1 882,934 + DZA65_RS04230 0.77 +0.7 882,935 - DZA65_RS04230 0.77 +0.6 882,969 + DZA65_RS04230 0.87 -1.0 882,969 + DZA65_RS04230 0.87 +0.4 882,969 + DZA65_RS04230 0.87 +0.1 882,969 + DZA65_RS04230 0.87 +1.0 882,969 + DZA65_RS04230 0.87 +0.6 882,969 + DZA65_RS04230 0.87 -0.9 882,969 + DZA65_RS04230 0.87 +1.1 882,969 + DZA65_RS04230 0.87 -0.5 882,970 - DZA65_RS04230 0.87 -0.6 882,970 - DZA65_RS04230 0.87 +0.6 882,970 - DZA65_RS04230 0.87 -1.5 883,005 + +1.7 883,014 + +0.0 883,021 + -0.9 883,021 + +0.6 883,021 + +1.3 883,021 + -0.2 883,021 + -1.5 883,021 + +1.9 883,021 + -1.7 883,021 + +0.2 883,022 - +0.1 883,022 - +0.7 883,022 - +0.3 883,022 - -1.3 883,022 - -0.5 883,022 - -0.5 883,022 - +0.3 883,038 + +0.1 883,038 + +4.1 883,038 + +0.4 883,039 - +0.4 883,052 + +0.9 883,052 + +0.2 883,052 + +0.2 883,052 + -0.5 883,053 - -1.3 883,053 - +1.1 883,053 - -0.3 883,055 + -1.3 883,055 + -0.2 883,055 + -1.5 883,055 + -0.2 883,055 + +0.2 883,056 - +0.2 883,056 - -0.5 883,056 - -0.8 883,056 - +0.4 883,056 - -2.1 883,056 - +2.8 883,056 - -0.7 883,056 - +0.3 883,056 - +0.1 883,056 - +0.3 883,093 - -0.5 883,127 + -0.1 883,128 - -0.5 883,269 + +0.1 883,270 - -0.3 883,280 + -0.9 883,280 + -0.3 883,280 + +0.0 883,280 + -0.1 883,281 - -1.5 883,281 - -0.5 883,281 - -0.5 883,281 - -1.9 883,281 - -1.3 883,281 - -1.7 883,281 - +2.1 883,339 - DZA65_RS04235 0.13 +0.6 883,339 - DZA65_RS04235 0.13 +0.1 883,461 + DZA65_RS04235 0.20 +0.2 883,498 + DZA65_RS04235 0.22 -1.1 883,498 + DZA65_RS04235 0.22 -1.3 883,498 + DZA65_RS04235 0.22 +0.6 883,498 + DZA65_RS04235 0.22 -0.8 883,499 - DZA65_RS04235 0.22 -0.8 883,536 + DZA65_RS04235 0.25 +1.1 883,536 + DZA65_RS04235 0.25 -0.1 883,536 + DZA65_RS04235 0.25 -2.4 883,537 - DZA65_RS04235 0.25 -0.5 883,783 + DZA65_RS04235 0.40 +0.3 883,783 + DZA65_RS04235 0.40 +0.0 883,783 + DZA65_RS04235 0.40 -0.6 883,784 - DZA65_RS04235 0.40 +0.5 883,784 - DZA65_RS04235 0.40 +0.0 883,784 - DZA65_RS04235 0.40 +0.1 883,784 - DZA65_RS04235 0.40 -0.0 883,784 - DZA65_RS04235 0.40 -0.7 883,784 - DZA65_RS04235 0.40 +0.3 883,784 - DZA65_RS04235 0.40 +0.8 883,801 + DZA65_RS04235 0.41 +0.3 883,801 + DZA65_RS04235 0.41 -0.9 883,801 + DZA65_RS04235 0.41 +1.1 883,801 + DZA65_RS04235 0.41 -0.7 883,801 + DZA65_RS04235 0.41 +2.2 883,801 + DZA65_RS04235 0.41 -1.3 883,801 + DZA65_RS04235 0.41 -1.2 883,802 - DZA65_RS04235 0.41 +1.1 883,802 - DZA65_RS04235 0.41 -2.3 883,802 - DZA65_RS04235 0.41 -0.9 883,802 - DZA65_RS04235 0.41 -0.1 883,802 - DZA65_RS04235 0.41 -1.8 883,811 + DZA65_RS04235 0.41 +0.5 883,812 - DZA65_RS04235 0.41 -0.2 883,892 + DZA65_RS04235 0.46 -0.5 883,892 + DZA65_RS04235 0.46 -0.1 883,893 - DZA65_RS04235 0.46 -2.2 883,893 - DZA65_RS04235 0.46 +0.4 883,893 - DZA65_RS04235 0.46 -0.7 883,893 - DZA65_RS04235 0.46 -0.1 883,893 - DZA65_RS04235 0.46 +1.9 883,917 + DZA65_RS04235 0.48 +2.1 883,918 - DZA65_RS04235 0.48 -0.9 883,918 - DZA65_RS04235 0.48 +0.2 883,928 + DZA65_RS04235 0.48 +0.7 884,147 + DZA65_RS04235 0.61 -0.9 884,147 + DZA65_RS04235 0.61 -0.0 884,147 + DZA65_RS04235 0.61 +0.7 884,147 + DZA65_RS04235 0.61 -0.3 884,147 + DZA65_RS04235 0.61 +0.4 884,148 - DZA65_RS04235 0.61 +2.2 884,148 - DZA65_RS04235 0.61 -2.5 884,148 - DZA65_RS04235 0.61 -0.6 884,148 - DZA65_RS04235 0.61 +2.1 884,148 - DZA65_RS04235 0.61 -2.2 884,148 - DZA65_RS04235 0.61 -3.2 884,148 - DZA65_RS04235 0.61 -1.9 884,169 - DZA65_RS04235 0.63 -0.3 884,214 - DZA65_RS04235 0.65 +1.1 884,323 + DZA65_RS04235 0.72 -1.6 884,323 + DZA65_RS04235 0.72 -0.9 884,323 + DZA65_RS04235 0.72 -0.7 884,323 + DZA65_RS04235 0.72 +0.7 884,324 - DZA65_RS04235 0.72 -0.2 884,324 - DZA65_RS04235 0.72 -0.5 884,324 - DZA65_RS04235 0.72 +1.1 884,324 - DZA65_RS04235 0.72 +1.6 884,324 - +2.9 884,324 - DZA65_RS04235 0.72 -1.9 884,324 - DZA65_RS04235 0.72 -1.5 884,377 + DZA65_RS04235 0.75 +1.1 884,377 + DZA65_RS04235 0.75 -1.3 884,378 - DZA65_RS04235 0.75 +0.5 884,378 - DZA65_RS04235 0.75 -1.9 884,378 - DZA65_RS04235 0.75 +1.3 884,571 + DZA65_RS04235 0.87 +0.6 884,571 + DZA65_RS04235 0.87 -1.3 884,572 - DZA65_RS04235 0.87 -1.7 884,572 - DZA65_RS04235 0.87 -0.9 884,673 + -1.0 884,673 + -1.7 884,673 + +0.9 884,673 + +1.1 884,674 - -0.1 884,674 - +0.9 884,674 - -1.7 884,763 + +0.3 884,764 - -0.2 884,790 + +0.7 884,790 + +0.8 884,791 - +0.1 884,800 + -1.9 884,800 + +0.6 884,801 - +0.7 884,801 - -0.3 884,811 - -0.5 884,811 - +1.4 884,820 + -0.7 884,821 - +0.6 884,824 + -0.1 884,825 - +0.2 884,829 + +0.5 884,831 + -1.3 884,847 - -1.3 884,866 + +1.3 884,866 + -0.2 885,761 - +0.2 885,761 - -1.5 885,761 - -0.5 885,761 - -0.4 885,761 - -0.8 885,761 - +0.5 885,761 - +2.1 885,761 - -0.2 885,761 - -3.2 885,761 - +0.1
Or see this region's nucleotide sequence