Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01010 and DZA65_RS01015 are separated by 138 nucleotides DZA65_RS01015 and DZA65_RS01020 are separated by 36 nucleotides DZA65_RS01020 and DZA65_RS01025 are separated by 25 nucleotides DZA65_RS01025 and DZA65_RS01030 are separated by 52 nucleotides
DZA65_RS01010: DZA65_RS01010 - FAD-dependent oxidoreductase, at 229,372 to 231,045
_RS01010
DZA65_RS01015: DZA65_RS01015 - helix-turn-helix transcriptional regulator, at 231,184 to 231,501
_RS01015
DZA65_RS01020: DZA65_RS01020 - YeeE/YedE family protein, at 231,538 to 231,966
_RS01020
DZA65_RS01025: DZA65_RS01025 - membrane protein, at 231,992 to 232,420
_RS01025
DZA65_RS01030: DZA65_RS01030 - acetyl-CoA C-acyltransferase FadA, at 232,473 to 233,636
_RS01030
Position (kb) 231
232
233 Strain fitness (log2 ratio) -2
-1
0
1
2 at 231.033 kb on + strand at 231.033 kb on + strand at 231.033 kb on + strand at 231.033 kb on + strand at 231.033 kb on + strand at 231.034 kb on - strand at 231.034 kb on - strand at 231.034 kb on - strand at 231.065 kb on - strand at 231.098 kb on + strand at 231.183 kb on + strand at 231.438 kb on + strand, within DZA65_RS01015 at 231.439 kb on - strand, within DZA65_RS01015 at 231.468 kb on + strand, within DZA65_RS01015 at 231.468 kb on + strand, within DZA65_RS01015 at 231.468 kb on + strand, within DZA65_RS01015 at 231.468 kb on + strand, within DZA65_RS01015 at 231.469 kb on - strand, within DZA65_RS01015 at 231.469 kb on - strand, within DZA65_RS01015 at 231.469 kb on - strand, within DZA65_RS01015 at 231.469 kb on - strand, within DZA65_RS01015 at 231.469 kb on - strand, within DZA65_RS01015 at 231.471 kb on + strand at 231.471 kb on + strand at 231.471 kb on + strand at 231.471 kb on + strand at 231.472 kb on - strand at 231.472 kb on - strand at 231.472 kb on - strand at 231.472 kb on - strand at 231.472 kb on - strand at 231.478 kb on + strand at 231.478 kb on + strand at 231.478 kb on + strand at 231.479 kb on - strand at 231.479 kb on - strand at 231.479 kb on - strand at 231.479 kb on - strand at 231.479 kb on - strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.487 kb on + strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.488 kb on - strand at 231.499 kb on + strand at 231.499 kb on + strand at 231.499 kb on + strand at 231.500 kb on - strand at 231.500 kb on - strand at 231.500 kb on - strand at 231.549 kb on + strand at 231.550 kb on - strand at 231.550 kb on - strand at 231.588 kb on + strand, within DZA65_RS01020 at 231.589 kb on - strand, within DZA65_RS01020 at 231.624 kb on - strand, within DZA65_RS01020 at 231.776 kb on + strand, within DZA65_RS01020 at 232.002 kb on + strand at 232.002 kb on + strand at 232.002 kb on + strand at 232.002 kb on + strand at 232.003 kb on - strand at 232.003 kb on - strand at 232.003 kb on - strand at 232.091 kb on - strand, within DZA65_RS01025 at 232.219 kb on - strand, within DZA65_RS01025 at 232.234 kb on + strand, within DZA65_RS01025 at 232.323 kb on - strand, within DZA65_RS01025 at 232.323 kb on - strand, within DZA65_RS01025 at 232.379 kb on + strand at 232.379 kb on + strand at 232.379 kb on + strand at 232.379 kb on + strand at 232.379 kb on + strand at 232.379 kb on + strand at 232.380 kb on - strand at 232.380 kb on - strand at 232.380 kb on - strand at 232.380 kb on - strand at 232.380 kb on - strand at 232.380 kb on - strand at 232.393 kb on + strand at 232.474 kb on + strand at 232.475 kb on - strand at 232.476 kb on + strand at 232.477 kb on - strand at 232.517 kb on + strand at 232.518 kb on - strand at 232.518 kb on - strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.523 kb on + strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.524 kb on - strand at 232.565 kb on + strand at 232.646 kb on + strand, within DZA65_RS01030 at 232.740 kb on + strand, within DZA65_RS01030 at 232.740 kb on + strand, within DZA65_RS01030 at 232.761 kb on + strand, within DZA65_RS01030 at 232.762 kb on - strand, within DZA65_RS01030 at 232.762 kb on - strand, within DZA65_RS01030 at 232.762 kb on - strand, within DZA65_RS01030 at 232.991 kb on + strand, within DZA65_RS01030 at 233.021 kb on - strand, within DZA65_RS01030 at 233.069 kb on + strand, within DZA65_RS01030 at 233.213 kb on + strand, within DZA65_RS01030 at 233.416 kb on - strand, within DZA65_RS01030 at 233.416 kb on - strand, within DZA65_RS01030
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 231,033 + +1.0 231,033 + +0.1 231,033 + -0.0 231,033 + -0.3 231,033 + +0.2 231,034 - +0.1 231,034 - +0.5 231,034 - -0.8 231,065 - -2.3 231,098 + -1.5 231,183 + -0.5 231,438 + DZA65_RS01015 0.80 -1.3 231,439 - DZA65_RS01015 0.80 +0.2 231,468 + DZA65_RS01015 0.89 -2.4 231,468 + DZA65_RS01015 0.89 +0.0 231,468 + DZA65_RS01015 0.89 +2.5 231,468 + DZA65_RS01015 0.89 +1.1 231,469 - DZA65_RS01015 0.90 +0.0 231,469 - DZA65_RS01015 0.90 -0.4 231,469 - DZA65_RS01015 0.90 -0.1 231,469 - DZA65_RS01015 0.90 -1.5 231,469 - DZA65_RS01015 0.90 +0.9 231,471 + -2.4 231,471 + -0.1 231,471 + -0.2 231,471 + -0.6 231,472 - -1.0 231,472 - +1.3 231,472 - -1.1 231,472 - -2.4 231,472 - +0.0 231,478 + -1.7 231,478 + +1.0 231,478 + +1.3 231,479 - -0.1 231,479 - -0.3 231,479 - +0.4 231,479 - +1.0 231,479 - +0.5 231,487 + +0.6 231,487 + -1.3 231,487 + -0.3 231,487 + +0.3 231,487 + +0.3 231,487 + +0.5 231,487 + -1.1 231,488 - +0.5 231,488 - -0.2 231,488 - +0.3 231,488 - -0.5 231,488 - +0.0 231,488 - +0.7 231,488 - +0.3 231,488 - +0.3 231,488 - -0.9 231,499 + -1.5 231,499 + -0.3 231,499 + -0.0 231,500 - +0.9 231,500 - +1.0 231,500 - -0.5 231,549 + -1.1 231,550 - -0.0 231,550 - +0.0 231,588 + DZA65_RS01020 0.12 +0.7 231,589 - DZA65_RS01020 0.12 -0.1 231,624 - DZA65_RS01020 0.20 -0.3 231,776 + DZA65_RS01020 0.55 +0.7 232,002 + -0.0 232,002 + +0.0 232,002 + -1.7 232,002 + -1.0 232,003 - -0.5 232,003 - +0.6 232,003 - +0.1 232,091 - DZA65_RS01025 0.23 +0.3 232,219 - DZA65_RS01025 0.53 +0.5 232,234 + DZA65_RS01025 0.56 +1.5 232,323 - DZA65_RS01025 0.77 +2.1 232,323 - DZA65_RS01025 0.77 -0.5 232,379 + -0.8 232,379 + +1.1 232,379 + +0.3 232,379 + +0.8 232,379 + -1.2 232,379 + -1.0 232,380 - +1.1 232,380 - +1.8 232,380 - +1.1 232,380 - +0.3 232,380 - -1.6 232,380 - -1.5 232,393 + -0.0 232,474 + +0.1 232,475 - -1.1 232,476 + +0.1 232,477 - -0.8 232,517 + +0.6 232,518 - +0.6 232,518 - +1.0 232,523 + -1.0 232,523 + +0.3 232,523 + +0.6 232,523 + +0.0 232,523 + +0.1 232,523 + -0.3 232,523 + +0.3 232,523 + +1.3 232,523 + +1.3 232,523 + +0.4 232,523 + +1.3 232,524 - -0.7 232,524 - -1.0 232,524 - -0.4 232,524 - -0.2 232,524 - -0.3 232,524 - -0.3 232,524 - -0.4 232,565 + +0.2 232,646 + DZA65_RS01030 0.15 +0.6 232,740 + DZA65_RS01030 0.23 -1.0 232,740 + DZA65_RS01030 0.23 -1.2 232,761 + DZA65_RS01030 0.25 -1.1 232,762 - DZA65_RS01030 0.25 -0.8 232,762 - DZA65_RS01030 0.25 -1.5 232,762 - DZA65_RS01030 0.25 +1.0 232,991 + DZA65_RS01030 0.45 +0.1 233,021 - DZA65_RS01030 0.47 -0.8 233,069 + DZA65_RS01030 0.51 -0.4 233,213 + DZA65_RS01030 0.64 +0.3 233,416 - DZA65_RS01030 0.81 -0.1 233,416 - DZA65_RS01030 0.81 -0.1
Or see this region's nucleotide sequence