Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS00830 and DZA65_RS00835 are separated by 2 nucleotides DZA65_RS00835 and DZA65_RS00840 are separated by 226 nucleotides DZA65_RS00840 and DZA65_RS00845 are separated by 99 nucleotides
DZA65_RS00830: DZA65_RS00830 - divergent polysaccharide deacetylase family protein, at 186,308 to 187,297
_RS00830
DZA65_RS00835: DZA65_RS00835 - murein hydrolase activator EnvC, at 187,300 to 188,574
_RS00835
DZA65_RS00840: DZA65_RS00840 - rhodanese-like domain-containing protein, at 188,801 to 189,232
_RS00840
DZA65_RS00845: DZA65_RS00845 - protein-export chaperone SecB, at 189,332 to 189,802
_RS00845
Position (kb) 187
188
189 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 186.342 kb on + strand at 186.342 kb on + strand at 186.342 kb on + strand at 186.343 kb on - strand at 186.370 kb on + strand at 186.370 kb on + strand at 186.371 kb on - strand at 186.371 kb on - strand at 186.371 kb on - strand at 186.389 kb on + strand at 186.389 kb on + strand at 186.389 kb on + strand at 186.390 kb on - strand at 186.390 kb on - strand at 186.390 kb on - strand at 186.407 kb on - strand, within DZA65_RS00830 at 186.407 kb on - strand, within DZA65_RS00830 at 186.489 kb on + strand, within DZA65_RS00830 at 186.489 kb on + strand, within DZA65_RS00830 at 186.489 kb on + strand, within DZA65_RS00830 at 186.489 kb on + strand, within DZA65_RS00830 at 186.490 kb on - strand, within DZA65_RS00830 at 186.490 kb on - strand, within DZA65_RS00830 at 186.490 kb on - strand, within DZA65_RS00830 at 186.508 kb on - strand, within DZA65_RS00830 at 186.508 kb on - strand, within DZA65_RS00830 at 186.650 kb on + strand, within DZA65_RS00830 at 186.650 kb on + strand, within DZA65_RS00830 at 186.651 kb on - strand, within DZA65_RS00830 at 186.651 kb on - strand, within DZA65_RS00830 at 186.651 kb on - strand, within DZA65_RS00830 at 186.724 kb on + strand, within DZA65_RS00830 at 186.724 kb on + strand, within DZA65_RS00830 at 186.725 kb on - strand, within DZA65_RS00830 at 186.799 kb on - strand, within DZA65_RS00830 at 186.871 kb on + strand, within DZA65_RS00830 at 186.872 kb on - strand, within DZA65_RS00830 at 186.895 kb on - strand, within DZA65_RS00830 at 187.063 kb on - strand, within DZA65_RS00830 at 187.228 kb on + strand at 187.228 kb on + strand at 187.228 kb on + strand at 187.229 kb on - strand at 187.229 kb on - strand at 187.258 kb on + strand at 187.258 kb on + strand at 187.258 kb on + strand at 187.259 kb on - strand at 187.259 kb on - strand at 187.259 kb on - strand at 187.286 kb on + strand at 187.286 kb on + strand at 187.286 kb on + strand at 187.287 kb on - strand at 187.301 kb on + strand at 187.302 kb on - strand at 187.302 kb on - strand at 187.302 kb on - strand at 187.302 kb on - strand at 187.309 kb on + strand at 187.310 kb on - strand at 187.310 kb on - strand at 187.358 kb on + strand at 187.456 kb on - strand, within DZA65_RS00835 at 187.457 kb on + strand, within DZA65_RS00835 at 187.457 kb on + strand, within DZA65_RS00835 at 187.457 kb on + strand, within DZA65_RS00835 at 187.463 kb on + strand, within DZA65_RS00835 at 187.464 kb on - strand, within DZA65_RS00835 at 187.464 kb on - strand, within DZA65_RS00835 at 187.464 kb on - strand, within DZA65_RS00835 at 187.464 kb on - strand, within DZA65_RS00835 at 187.512 kb on - strand, within DZA65_RS00835 at 187.512 kb on - strand, within DZA65_RS00835 at 187.562 kb on - strand, within DZA65_RS00835 at 187.667 kb on + strand, within DZA65_RS00835 at 187.667 kb on + strand, within DZA65_RS00835 at 187.667 kb on + strand, within DZA65_RS00835 at 187.668 kb on - strand at 187.668 kb on - strand, within DZA65_RS00835 at 187.668 kb on - strand, within DZA65_RS00835 at 187.668 kb on - strand, within DZA65_RS00835 at 187.668 kb on - strand, within DZA65_RS00835 at 187.668 kb on - strand, within DZA65_RS00835 at 187.668 kb on - strand, within DZA65_RS00835 at 187.734 kb on + strand, within DZA65_RS00835 at 187.734 kb on + strand, within DZA65_RS00835 at 187.734 kb on + strand, within DZA65_RS00835 at 187.734 kb on + strand, within DZA65_RS00835 at 187.735 kb on - strand, within DZA65_RS00835 at 187.735 kb on - strand, within DZA65_RS00835 at 187.735 kb on - strand, within DZA65_RS00835 at 187.736 kb on + strand, within DZA65_RS00835 at 187.736 kb on + strand, within DZA65_RS00835 at 187.736 kb on + strand, within DZA65_RS00835 at 187.736 kb on + strand, within DZA65_RS00835 at 187.736 kb on + strand, within DZA65_RS00835 at 187.736 kb on + strand, within DZA65_RS00835 at 187.736 kb on + strand, within DZA65_RS00835 at 187.736 kb on + strand, within DZA65_RS00835 at 187.736 kb on + strand, within DZA65_RS00835 at 187.736 kb on + strand, within DZA65_RS00835 at 187.737 kb on - strand, within DZA65_RS00835 at 187.737 kb on - strand, within DZA65_RS00835 at 187.737 kb on - strand, within DZA65_RS00835 at 187.737 kb on - strand, within DZA65_RS00835 at 187.737 kb on - strand, within DZA65_RS00835 at 188.099 kb on + strand, within DZA65_RS00835 at 188.099 kb on + strand, within DZA65_RS00835 at 188.099 kb on + strand, within DZA65_RS00835 at 188.100 kb on - strand, within DZA65_RS00835 at 188.100 kb on - strand, within DZA65_RS00835 at 188.100 kb on - strand, within DZA65_RS00835 at 188.109 kb on - strand, within DZA65_RS00835 at 188.488 kb on + strand at 188.521 kb on + strand at 188.522 kb on - strand at 188.522 kb on - strand at 188.585 kb on + strand at 188.585 kb on + strand at 188.585 kb on + strand at 188.586 kb on - strand at 188.586 kb on - strand at 188.586 kb on - strand at 188.632 kb on + strand at 188.632 kb on + strand at 188.633 kb on - strand at 188.673 kb on + strand at 188.693 kb on + strand at 188.693 kb on + strand at 188.716 kb on - strand at 188.724 kb on + strand at 188.724 kb on + strand at 188.724 kb on + strand at 188.725 kb on - strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.740 kb on + strand at 188.741 kb on - strand at 188.741 kb on - strand at 188.741 kb on - strand at 188.741 kb on - strand at 188.741 kb on - strand at 188.741 kb on - strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.744 kb on + strand at 188.745 kb on - strand at 188.746 kb on + strand at 188.746 kb on + strand at 188.746 kb on + strand at 188.761 kb on + strand at 188.761 kb on + strand at 188.761 kb on + strand at 188.761 kb on + strand at 188.774 kb on + strand at 188.774 kb on + strand at 188.774 kb on + strand at 188.774 kb on + strand at 188.774 kb on + strand at 188.793 kb on + strand at 188.812 kb on + strand at 188.812 kb on + strand at 188.812 kb on + strand at 188.812 kb on + strand at 188.812 kb on + strand at 188.813 kb on - strand at 188.884 kb on + strand, within DZA65_RS00840 at 188.896 kb on + strand, within DZA65_RS00840 at 188.896 kb on + strand, within DZA65_RS00840 at 188.897 kb on - strand, within DZA65_RS00840 at 188.947 kb on + strand, within DZA65_RS00840 at 188.948 kb on - strand, within DZA65_RS00840 at 188.950 kb on + strand, within DZA65_RS00840 at 188.970 kb on + strand, within DZA65_RS00840 at 189.007 kb on + strand, within DZA65_RS00840 at 189.007 kb on + strand, within DZA65_RS00840 at 189.007 kb on + strand, within DZA65_RS00840 at 189.008 kb on - strand, within DZA65_RS00840 at 189.267 kb on + strand at 189.267 kb on + strand at 189.376 kb on + strand at 189.377 kb on - strand at 189.380 kb on + strand, within DZA65_RS00845
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 186,342 + -0.4 186,342 + +1.1 186,342 + +0.3 186,343 - -1.8 186,370 + -0.2 186,370 + -1.3 186,371 - +0.9 186,371 - +1.2 186,371 - +0.3 186,389 + +3.3 186,389 + -0.7 186,389 + +0.0 186,390 - +1.5 186,390 - +1.1 186,390 - +0.7 186,407 - DZA65_RS00830 0.10 +1.1 186,407 - DZA65_RS00830 0.10 +0.6 186,489 + DZA65_RS00830 0.18 +0.2 186,489 + DZA65_RS00830 0.18 +0.8 186,489 + DZA65_RS00830 0.18 +1.2 186,489 + DZA65_RS00830 0.18 +1.7 186,490 - DZA65_RS00830 0.18 +1.3 186,490 - DZA65_RS00830 0.18 +1.1 186,490 - DZA65_RS00830 0.18 -2.1 186,508 - DZA65_RS00830 0.20 -0.5 186,508 - DZA65_RS00830 0.20 +1.2 186,650 + DZA65_RS00830 0.35 +0.6 186,650 + DZA65_RS00830 0.35 -0.4 186,651 - DZA65_RS00830 0.35 +1.3 186,651 - DZA65_RS00830 0.35 +0.9 186,651 - DZA65_RS00830 0.35 +0.2 186,724 + DZA65_RS00830 0.42 -1.0 186,724 + DZA65_RS00830 0.42 -0.3 186,725 - DZA65_RS00830 0.42 +1.3 186,799 - DZA65_RS00830 0.50 -0.8 186,871 + DZA65_RS00830 0.57 +1.1 186,872 - DZA65_RS00830 0.57 -1.0 186,895 - DZA65_RS00830 0.59 +0.1 187,063 - DZA65_RS00830 0.76 -0.3 187,228 + +0.7 187,228 + +0.8 187,228 + -0.8 187,229 - -0.0 187,229 - +1.1 187,258 + +0.1 187,258 + +0.0 187,258 + +0.6 187,259 - -0.4 187,259 - -0.5 187,259 - -0.8 187,286 + +0.1 187,286 + +0.2 187,286 + +0.7 187,287 - +0.7 187,301 + +1.1 187,302 - +0.3 187,302 - -0.6 187,302 - +0.9 187,302 - +2.6 187,309 + +0.3 187,310 - -1.3 187,310 - +1.3 187,358 + -1.5 187,456 - DZA65_RS00835 0.12 +0.4 187,457 + DZA65_RS00835 0.12 +1.1 187,457 + DZA65_RS00835 0.12 +1.3 187,457 + DZA65_RS00835 0.12 +1.3 187,463 + DZA65_RS00835 0.13 +0.1 187,464 - DZA65_RS00835 0.13 +0.5 187,464 - DZA65_RS00835 0.13 +0.3 187,464 - DZA65_RS00835 0.13 +1.0 187,464 - DZA65_RS00835 0.13 +2.2 187,512 - DZA65_RS00835 0.17 +2.2 187,512 - DZA65_RS00835 0.17 +1.3 187,562 - DZA65_RS00835 0.21 +1.9 187,667 + DZA65_RS00835 0.29 +0.8 187,667 + DZA65_RS00835 0.29 +1.3 187,667 + DZA65_RS00835 0.29 +0.7 187,668 - +3.8 187,668 - DZA65_RS00835 0.29 +0.4 187,668 - DZA65_RS00835 0.29 +0.0 187,668 - DZA65_RS00835 0.29 +2.8 187,668 - DZA65_RS00835 0.29 +2.6 187,668 - DZA65_RS00835 0.29 +2.1 187,668 - DZA65_RS00835 0.29 -1.0 187,734 + DZA65_RS00835 0.34 -0.3 187,734 + DZA65_RS00835 0.34 +2.3 187,734 + DZA65_RS00835 0.34 +3.3 187,734 + DZA65_RS00835 0.34 -0.5 187,735 - DZA65_RS00835 0.34 +2.6 187,735 - DZA65_RS00835 0.34 +2.3 187,735 - DZA65_RS00835 0.34 -1.1 187,736 + DZA65_RS00835 0.34 +0.7 187,736 + DZA65_RS00835 0.34 +0.7 187,736 + DZA65_RS00835 0.34 +1.4 187,736 + DZA65_RS00835 0.34 +0.0 187,736 + DZA65_RS00835 0.34 +0.7 187,736 + DZA65_RS00835 0.34 +0.7 187,736 + DZA65_RS00835 0.34 -1.5 187,736 + DZA65_RS00835 0.34 +2.3 187,736 + DZA65_RS00835 0.34 -0.5 187,736 + DZA65_RS00835 0.34 +0.8 187,737 - DZA65_RS00835 0.34 +2.0 187,737 - DZA65_RS00835 0.34 +1.2 187,737 - DZA65_RS00835 0.34 -0.3 187,737 - DZA65_RS00835 0.34 -1.5 187,737 - DZA65_RS00835 0.34 +1.9 188,099 + DZA65_RS00835 0.63 +2.5 188,099 + DZA65_RS00835 0.63 +2.3 188,099 + DZA65_RS00835 0.63 +0.7 188,100 - DZA65_RS00835 0.63 +1.3 188,100 - DZA65_RS00835 0.63 +1.3 188,100 - DZA65_RS00835 0.63 +0.0 188,109 - DZA65_RS00835 0.63 +1.6 188,488 + +1.4 188,521 + +1.9 188,522 - +1.6 188,522 - +1.0 188,585 + -1.3 188,585 + +0.6 188,585 + +0.5 188,586 - +0.4 188,586 - -1.6 188,586 - -1.1 188,632 + -0.6 188,632 + -1.1 188,633 - +0.7 188,673 + +0.6 188,693 + +0.2 188,693 + -1.8 188,716 - -0.2 188,724 + +0.5 188,724 + -0.5 188,724 + -0.7 188,725 - -0.5 188,740 + +0.3 188,740 + -0.4 188,740 + -0.5 188,740 + -1.8 188,740 + +0.5 188,740 + -0.1 188,740 + -1.5 188,741 - -0.3 188,741 - +0.5 188,741 - -0.7 188,741 - +0.0 188,741 - +0.9 188,741 - -0.2 188,744 + -1.3 188,744 + +0.6 188,744 + -2.0 188,744 + +0.7 188,744 + +0.1 188,744 + +0.6 188,744 + -1.7 188,744 + +1.9 188,745 - -0.3 188,746 + -1.3 188,746 + -0.5 188,746 + +1.3 188,761 + +0.2 188,761 + -0.3 188,761 + +0.1 188,761 + +0.6 188,774 + +1.7 188,774 + -0.1 188,774 + -0.3 188,774 + -1.3 188,774 + +2.0 188,793 + -1.0 188,812 + +0.6 188,812 + -1.4 188,812 + +0.7 188,812 + -0.5 188,812 + -0.5 188,813 - -0.5 188,884 + DZA65_RS00840 0.19 -0.9 188,896 + DZA65_RS00840 0.22 +0.7 188,896 + DZA65_RS00840 0.22 -0.8 188,897 - DZA65_RS00840 0.22 -1.0 188,947 + DZA65_RS00840 0.34 -0.6 188,948 - DZA65_RS00840 0.34 +0.3 188,950 + DZA65_RS00840 0.34 +0.7 188,970 + DZA65_RS00840 0.39 -0.3 189,007 + DZA65_RS00840 0.48 -1.5 189,007 + DZA65_RS00840 0.48 +0.3 189,007 + DZA65_RS00840 0.48 +0.6 189,008 - DZA65_RS00840 0.48 +0.3 189,267 + -1.7 189,267 + +0.3 189,376 + -1.3 189,377 - -0.7 189,380 + DZA65_RS00845 0.10 -0.7
Or see this region's nucleotide sequence