Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10285 and DZA65_RS10290 are separated by 101 nucleotides DZA65_RS10290 and DZA65_RS10295 are separated by 18 nucleotides DZA65_RS10295 and DZA65_RS22865 are separated by 182 nucleotides
DZA65_RS10285: DZA65_RS10285 - LysR family transcriptional regulator, at 2,177,404 to 2,178,357
_RS10285
DZA65_RS10290: DZA65_RS10290 - carbon-nitrogen hydrolase family protein, at 2,178,459 to 2,179,385
_RS10290
DZA65_RS10295: DZA65_RS10295 - hypothetical protein, at 2,179,404 to 2,179,823
_RS10295
DZA65_RS22865: DZA65_RS22865 - hypothetical protein, at 2,180,006 to 2,180,449
_RS22865
Position (kb)
2178
2179
2180 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2177.549 kb on + strand at 2177.549 kb on + strand, within DZA65_RS10285 at 2177.818 kb on - strand at 2177.858 kb on - strand, within DZA65_RS10285 at 2178.053 kb on - strand, within DZA65_RS10285 at 2178.068 kb on + strand, within DZA65_RS10285 at 2178.069 kb on - strand, within DZA65_RS10285 at 2178.069 kb on - strand, within DZA65_RS10285 at 2178.296 kb on + strand at 2178.296 kb on + strand at 2178.296 kb on + strand at 2178.296 kb on + strand at 2178.297 kb on - strand at 2178.297 kb on - strand at 2178.297 kb on - strand at 2178.297 kb on - strand at 2178.346 kb on + strand at 2178.347 kb on - strand at 2178.347 kb on - strand at 2178.357 kb on + strand at 2178.408 kb on + strand at 2178.408 kb on + strand at 2178.409 kb on - strand at 2178.409 kb on - strand at 2178.409 kb on - strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.453 kb on + strand at 2178.454 kb on - strand at 2178.454 kb on - strand at 2178.458 kb on + strand at 2178.459 kb on - strand at 2178.633 kb on + strand, within DZA65_RS10290 at 2178.634 kb on - strand at 2178.634 kb on - strand, within DZA65_RS10290 at 2178.634 kb on - strand, within DZA65_RS10290 at 2178.634 kb on - strand, within DZA65_RS10290 at 2178.678 kb on + strand, within DZA65_RS10290 at 2178.678 kb on + strand, within DZA65_RS10290 at 2178.679 kb on - strand, within DZA65_RS10290 at 2178.679 kb on - strand, within DZA65_RS10290 at 2178.681 kb on + strand, within DZA65_RS10290 at 2178.682 kb on - strand, within DZA65_RS10290 at 2178.793 kb on + strand, within DZA65_RS10290 at 2178.793 kb on + strand, within DZA65_RS10290 at 2178.793 kb on + strand, within DZA65_RS10290 at 2178.793 kb on + strand, within DZA65_RS10290 at 2178.793 kb on + strand, within DZA65_RS10290 at 2178.793 kb on + strand, within DZA65_RS10290 at 2178.793 kb on + strand, within DZA65_RS10290 at 2178.793 kb on + strand, within DZA65_RS10290 at 2178.793 kb on + strand, within DZA65_RS10290 at 2178.794 kb on - strand, within DZA65_RS10290 at 2178.794 kb on - strand, within DZA65_RS10290 at 2178.794 kb on - strand, within DZA65_RS10290 at 2178.794 kb on - strand, within DZA65_RS10290 at 2178.794 kb on - strand, within DZA65_RS10290 at 2178.794 kb on - strand, within DZA65_RS10290 at 2178.794 kb on - strand, within DZA65_RS10290 at 2178.794 kb on - strand, within DZA65_RS10290 at 2178.795 kb on + strand, within DZA65_RS10290 at 2178.795 kb on + strand, within DZA65_RS10290 at 2178.796 kb on - strand, within DZA65_RS10290 at 2178.796 kb on - strand, within DZA65_RS10290 at 2178.796 kb on - strand, within DZA65_RS10290 at 2178.929 kb on - strand at 2178.933 kb on - strand, within DZA65_RS10290 at 2178.981 kb on - strand, within DZA65_RS10290 at 2178.990 kb on + strand, within DZA65_RS10290 at 2178.990 kb on + strand, within DZA65_RS10290 at 2178.990 kb on + strand, within DZA65_RS10290 at 2178.990 kb on + strand, within DZA65_RS10290 at 2178.990 kb on + strand, within DZA65_RS10290 at 2178.990 kb on + strand, within DZA65_RS10290 at 2178.991 kb on - strand, within DZA65_RS10290 at 2178.991 kb on - strand, within DZA65_RS10290 at 2178.991 kb on - strand, within DZA65_RS10290 at 2178.991 kb on - strand, within DZA65_RS10290 at 2178.991 kb on - strand, within DZA65_RS10290 at 2179.095 kb on - strand, within DZA65_RS10290 at 2179.211 kb on - strand, within DZA65_RS10290 at 2179.371 kb on + strand at 2179.372 kb on - strand at 2179.372 kb on - strand at 2179.399 kb on - strand at 2179.405 kb on + strand at 2179.406 kb on - strand at 2179.406 kb on - strand at 2179.409 kb on + strand at 2179.409 kb on + strand at 2179.409 kb on + strand at 2179.412 kb on + strand at 2179.412 kb on + strand at 2179.435 kb on + strand at 2179.471 kb on - strand, within DZA65_RS10295 at 2179.474 kb on + strand at 2179.474 kb on + strand, within DZA65_RS10295 at 2179.474 kb on + strand, within DZA65_RS10295 at 2179.475 kb on - strand at 2179.479 kb on + strand, within DZA65_RS10295 at 2179.479 kb on + strand, within DZA65_RS10295 at 2179.479 kb on + strand, within DZA65_RS10295 at 2179.480 kb on - strand, within DZA65_RS10295 at 2179.480 kb on - strand, within DZA65_RS10295 at 2179.483 kb on + strand, within DZA65_RS10295 at 2179.483 kb on + strand, within DZA65_RS10295 at 2179.484 kb on - strand, within DZA65_RS10295 at 2179.493 kb on + strand, within DZA65_RS10295 at 2179.495 kb on + strand, within DZA65_RS10295 at 2179.496 kb on - strand, within DZA65_RS10295 at 2179.499 kb on + strand at 2179.499 kb on + strand, within DZA65_RS10295 at 2179.500 kb on - strand, within DZA65_RS10295 at 2179.521 kb on + strand, within DZA65_RS10295 at 2179.521 kb on + strand, within DZA65_RS10295 at 2179.521 kb on + strand, within DZA65_RS10295 at 2179.522 kb on - strand, within DZA65_RS10295 at 2179.522 kb on - strand, within DZA65_RS10295 at 2179.522 kb on - strand, within DZA65_RS10295 at 2179.583 kb on + strand, within DZA65_RS10295 at 2179.678 kb on + strand, within DZA65_RS10295 at 2179.678 kb on + strand, within DZA65_RS10295 at 2179.679 kb on - strand, within DZA65_RS10295 at 2179.740 kb on - strand, within DZA65_RS10295 at 2179.740 kb on - strand, within DZA65_RS10295 at 2179.741 kb on + strand, within DZA65_RS10295 at 2179.741 kb on + strand, within DZA65_RS10295 at 2179.744 kb on - strand, within DZA65_RS10295 at 2179.824 kb on - strand at 2179.940 kb on + strand at 2179.957 kb on + strand at 2179.958 kb on - strand at 2179.979 kb on - strand at 2179.997 kb on + strand at 2180.012 kb on - strand at 2180.012 kb on - strand at 2180.039 kb on - strand at 2180.064 kb on + strand, within DZA65_RS22865 at 2180.064 kb on + strand, within DZA65_RS22865 at 2180.064 kb on + strand, within DZA65_RS22865 at 2180.122 kb on + strand, within DZA65_RS22865 at 2180.199 kb on + strand, within DZA65_RS22865 at 2180.201 kb on + strand, within DZA65_RS22865 at 2180.223 kb on - strand, within DZA65_RS22865 at 2180.367 kb on - strand, within DZA65_RS22865
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,177,549 + -1.7 2,177,549 + DZA65_RS10285 0.15 -0.3 2,177,818 - +0.9 2,177,858 - DZA65_RS10285 0.48 -1.0 2,178,053 - DZA65_RS10285 0.68 -1.1 2,178,068 + DZA65_RS10285 0.70 -1.0 2,178,069 - DZA65_RS10285 0.70 +1.0 2,178,069 - DZA65_RS10285 0.70 -1.3 2,178,296 + +0.8 2,178,296 + -1.3 2,178,296 + +0.7 2,178,296 + -2.5 2,178,297 - -2.3 2,178,297 - +0.3 2,178,297 - +0.9 2,178,297 - +0.2 2,178,346 + -1.0 2,178,347 - -0.1 2,178,347 - -1.3 2,178,357 + +0.6 2,178,408 + +0.8 2,178,408 + -0.9 2,178,409 - +0.9 2,178,409 - +0.9 2,178,409 - +0.7 2,178,453 + -0.7 2,178,453 + -0.3 2,178,453 + -1.3 2,178,453 + +0.2 2,178,453 + +0.8 2,178,453 + +0.5 2,178,453 + +0.6 2,178,453 + -0.8 2,178,453 + -1.4 2,178,453 + +0.9 2,178,453 + -0.7 2,178,453 + -2.0 2,178,453 + +0.1 2,178,454 - +0.1 2,178,454 - -0.1 2,178,458 + +1.5 2,178,459 - +0.2 2,178,633 + DZA65_RS10290 0.19 -0.2 2,178,634 - +1.3 2,178,634 - DZA65_RS10290 0.19 +0.3 2,178,634 - DZA65_RS10290 0.19 -0.3 2,178,634 - DZA65_RS10290 0.19 -1.7 2,178,678 + DZA65_RS10290 0.24 +1.3 2,178,678 + DZA65_RS10290 0.24 -1.0 2,178,679 - DZA65_RS10290 0.24 -0.0 2,178,679 - DZA65_RS10290 0.24 -2.0 2,178,681 + DZA65_RS10290 0.24 -0.5 2,178,682 - DZA65_RS10290 0.24 +0.3 2,178,793 + DZA65_RS10290 0.36 -1.0 2,178,793 + DZA65_RS10290 0.36 +1.3 2,178,793 + DZA65_RS10290 0.36 +0.5 2,178,793 + DZA65_RS10290 0.36 -0.3 2,178,793 + DZA65_RS10290 0.36 +0.6 2,178,793 + DZA65_RS10290 0.36 -0.7 2,178,793 + DZA65_RS10290 0.36 -0.0 2,178,793 + DZA65_RS10290 0.36 +0.6 2,178,793 + DZA65_RS10290 0.36 -1.7 2,178,794 - DZA65_RS10290 0.36 -0.2 2,178,794 - DZA65_RS10290 0.36 +0.6 2,178,794 - DZA65_RS10290 0.36 -1.1 2,178,794 - DZA65_RS10290 0.36 -1.3 2,178,794 - DZA65_RS10290 0.36 -0.5 2,178,794 - DZA65_RS10290 0.36 -0.3 2,178,794 - DZA65_RS10290 0.36 -0.5 2,178,794 - DZA65_RS10290 0.36 -0.4 2,178,795 + DZA65_RS10290 0.36 +0.3 2,178,795 + DZA65_RS10290 0.36 -0.8 2,178,796 - DZA65_RS10290 0.36 +0.5 2,178,796 - DZA65_RS10290 0.36 -0.5 2,178,796 - DZA65_RS10290 0.36 -0.1 2,178,929 - +2.1 2,178,933 - DZA65_RS10290 0.51 +0.9 2,178,981 - DZA65_RS10290 0.56 -0.9 2,178,990 + DZA65_RS10290 0.57 -0.2 2,178,990 + DZA65_RS10290 0.57 -0.5 2,178,990 + DZA65_RS10290 0.57 +0.3 2,178,990 + DZA65_RS10290 0.57 -3.6 2,178,990 + DZA65_RS10290 0.57 -0.8 2,178,990 + DZA65_RS10290 0.57 -0.4 2,178,991 - DZA65_RS10290 0.57 +1.0 2,178,991 - DZA65_RS10290 0.57 +1.0 2,178,991 - DZA65_RS10290 0.57 -0.0 2,178,991 - DZA65_RS10290 0.57 -0.2 2,178,991 - DZA65_RS10290 0.57 -0.1 2,179,095 - DZA65_RS10290 0.69 -0.4 2,179,211 - DZA65_RS10290 0.81 -0.9 2,179,371 + -0.3 2,179,372 - -0.8 2,179,372 - +1.8 2,179,399 - +0.5 2,179,405 + +1.0 2,179,406 - -1.1 2,179,406 - -3.1 2,179,409 + -0.1 2,179,409 + -0.4 2,179,409 + +0.9 2,179,412 + +1.5 2,179,412 + +0.1 2,179,435 + +0.0 2,179,471 - DZA65_RS10295 0.16 -0.3 2,179,474 + -2.0 2,179,474 + DZA65_RS10295 0.17 +0.3 2,179,474 + DZA65_RS10295 0.17 -0.7 2,179,475 - +0.3 2,179,479 + DZA65_RS10295 0.18 -0.9 2,179,479 + DZA65_RS10295 0.18 +2.1 2,179,479 + DZA65_RS10295 0.18 -0.4 2,179,480 - DZA65_RS10295 0.18 +1.2 2,179,480 - DZA65_RS10295 0.18 +0.1 2,179,483 + DZA65_RS10295 0.19 -0.2 2,179,483 + DZA65_RS10295 0.19 +0.7 2,179,484 - DZA65_RS10295 0.19 -2.3 2,179,493 + DZA65_RS10295 0.21 +0.3 2,179,495 + DZA65_RS10295 0.22 +0.8 2,179,496 - DZA65_RS10295 0.22 -0.4 2,179,499 + +0.3 2,179,499 + DZA65_RS10295 0.23 +0.7 2,179,500 - DZA65_RS10295 0.23 +1.5 2,179,521 + DZA65_RS10295 0.28 -0.7 2,179,521 + DZA65_RS10295 0.28 +0.1 2,179,521 + DZA65_RS10295 0.28 -0.6 2,179,522 - DZA65_RS10295 0.28 +1.1 2,179,522 - DZA65_RS10295 0.28 +0.2 2,179,522 - DZA65_RS10295 0.28 +0.8 2,179,583 + DZA65_RS10295 0.43 +0.3 2,179,678 + DZA65_RS10295 0.65 -0.5 2,179,678 + DZA65_RS10295 0.65 -2.0 2,179,679 - DZA65_RS10295 0.65 +0.3 2,179,740 - DZA65_RS10295 0.80 +0.3 2,179,740 - DZA65_RS10295 0.80 -0.6 2,179,741 + DZA65_RS10295 0.80 +0.7 2,179,741 + DZA65_RS10295 0.80 -0.1 2,179,744 - DZA65_RS10295 0.81 +0.9 2,179,824 - -2.3 2,179,940 + -0.3 2,179,957 + +1.2 2,179,958 - -1.1 2,179,979 - +0.2 2,179,997 + -0.3 2,180,012 - -0.0 2,180,012 - -0.4 2,180,039 - -1.4 2,180,064 + DZA65_RS22865 0.13 +2.3 2,180,064 + DZA65_RS22865 0.13 -0.0 2,180,064 + DZA65_RS22865 0.13 +0.3 2,180,122 + DZA65_RS22865 0.26 +0.3 2,180,199 + DZA65_RS22865 0.43 +0.9 2,180,201 + DZA65_RS22865 0.44 -0.1 2,180,223 - DZA65_RS22865 0.49 +1.0 2,180,367 - DZA65_RS22865 0.81 +0.7
Or see this region's nucleotide sequence