Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS07735 and DZA65_RS07740 are separated by 154 nucleotides DZA65_RS07740 and DZA65_RS07745 are separated by 161 nucleotides DZA65_RS07745 and DZA65_RS07750 are separated by 198 nucleotides
DZA65_RS07735: DZA65_RS07735 - DUF736 domain-containing protein, at 1,609,648 to 1,609,959
_RS07735
DZA65_RS07740: DZA65_RS07740 - integrase arm-type DNA-binding domain-containing protein, at 1,610,114 to 1,611,397
_RS07740
DZA65_RS07745: DZA65_RS07745 - tRNA-Asn, at 1,611,559 to 1,611,634
_RS07745
DZA65_RS07750: DZA65_RS07750 - beta-galactosidase, at 1,611,833 to 1,614,964
_RS07750
Position (kb)
1610
1611
1612 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1609.121 kb on + strand at 1609.168 kb on + strand at 1609.168 kb on + strand at 1609.168 kb on + strand at 1609.168 kb on + strand at 1609.168 kb on + strand at 1609.169 kb on - strand at 1609.169 kb on - strand at 1609.205 kb on + strand at 1609.205 kb on + strand at 1609.206 kb on - strand at 1609.206 kb on - strand at 1609.206 kb on - strand at 1609.206 kb on - strand at 1609.223 kb on + strand at 1609.223 kb on + strand at 1609.223 kb on + strand at 1609.223 kb on + strand at 1609.223 kb on + strand at 1609.224 kb on - strand at 1609.224 kb on - strand at 1609.224 kb on - strand at 1609.268 kb on + strand at 1609.269 kb on - strand at 1609.296 kb on + strand at 1609.457 kb on + strand at 1609.457 kb on + strand at 1609.458 kb on - strand at 1609.542 kb on + strand at 1609.542 kb on + strand at 1609.543 kb on - strand at 1609.889 kb on - strand, within DZA65_RS07735 at 1609.889 kb on - strand, within DZA65_RS07735 at 1610.017 kb on + strand at 1610.017 kb on + strand at 1610.017 kb on + strand at 1610.018 kb on - strand at 1610.018 kb on - strand at 1610.018 kb on - strand at 1610.042 kb on - strand at 1610.067 kb on + strand at 1610.067 kb on + strand at 1610.067 kb on + strand at 1610.067 kb on + strand at 1610.067 kb on + strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.068 kb on - strand at 1610.126 kb on - strand at 1610.166 kb on + strand at 1610.178 kb on + strand at 1610.178 kb on + strand at 1610.194 kb on + strand at 1610.202 kb on + strand at 1610.202 kb on + strand at 1610.203 kb on - strand at 1610.244 kb on + strand, within DZA65_RS07740 at 1610.254 kb on + strand, within DZA65_RS07740 at 1610.255 kb on - strand, within DZA65_RS07740 at 1610.263 kb on - strand, within DZA65_RS07740 at 1610.263 kb on - strand, within DZA65_RS07740 at 1610.263 kb on - strand, within DZA65_RS07740 at 1610.400 kb on + strand, within DZA65_RS07740 at 1610.400 kb on + strand, within DZA65_RS07740 at 1610.401 kb on - strand, within DZA65_RS07740 at 1610.449 kb on + strand, within DZA65_RS07740 at 1610.449 kb on + strand, within DZA65_RS07740 at 1610.450 kb on - strand, within DZA65_RS07740 at 1610.451 kb on + strand, within DZA65_RS07740 at 1610.452 kb on - strand, within DZA65_RS07740 at 1610.452 kb on - strand, within DZA65_RS07740 at 1610.458 kb on + strand, within DZA65_RS07740 at 1610.459 kb on - strand, within DZA65_RS07740 at 1610.474 kb on + strand, within DZA65_RS07740 at 1610.475 kb on - strand, within DZA65_RS07740 at 1610.475 kb on - strand, within DZA65_RS07740 at 1610.505 kb on - strand, within DZA65_RS07740 at 1610.505 kb on - strand, within DZA65_RS07740 at 1610.505 kb on - strand, within DZA65_RS07740 at 1610.518 kb on + strand, within DZA65_RS07740 at 1610.549 kb on - strand, within DZA65_RS07740 at 1610.742 kb on + strand, within DZA65_RS07740 at 1610.742 kb on + strand, within DZA65_RS07740 at 1610.742 kb on + strand at 1610.742 kb on + strand, within DZA65_RS07740 at 1610.743 kb on - strand, within DZA65_RS07740 at 1610.743 kb on - strand, within DZA65_RS07740 at 1610.743 kb on - strand, within DZA65_RS07740 at 1610.743 kb on - strand, within DZA65_RS07740 at 1610.743 kb on - strand, within DZA65_RS07740 at 1610.756 kb on - strand, within DZA65_RS07740 at 1610.855 kb on + strand, within DZA65_RS07740 at 1610.856 kb on - strand, within DZA65_RS07740 at 1610.856 kb on - strand, within DZA65_RS07740 at 1610.877 kb on + strand, within DZA65_RS07740 at 1611.095 kb on + strand, within DZA65_RS07740 at 1611.095 kb on + strand, within DZA65_RS07740 at 1611.096 kb on - strand, within DZA65_RS07740 at 1611.096 kb on - strand, within DZA65_RS07740 at 1611.096 kb on - strand, within DZA65_RS07740 at 1611.163 kb on + strand, within DZA65_RS07740 at 1611.164 kb on - strand, within DZA65_RS07740 at 1611.164 kb on - strand, within DZA65_RS07740 at 1611.221 kb on + strand, within DZA65_RS07740 at 1611.221 kb on + strand, within DZA65_RS07740 at 1611.221 kb on + strand, within DZA65_RS07740 at 1611.221 kb on + strand, within DZA65_RS07740 at 1611.221 kb on + strand, within DZA65_RS07740 at 1611.221 kb on + strand, within DZA65_RS07740 at 1611.221 kb on + strand, within DZA65_RS07740 at 1611.222 kb on - strand, within DZA65_RS07740 at 1611.231 kb on + strand, within DZA65_RS07740 at 1611.231 kb on + strand, within DZA65_RS07740 at 1611.235 kb on + strand, within DZA65_RS07740 at 1611.235 kb on + strand, within DZA65_RS07740 at 1611.240 kb on - strand, within DZA65_RS07740 at 1611.269 kb on - strand at 1611.269 kb on - strand at 1611.274 kb on - strand at 1611.274 kb on - strand at 1611.274 kb on - strand at 1611.318 kb on + strand at 1611.318 kb on + strand at 1611.318 kb on + strand at 1611.365 kb on + strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.389 kb on - strand at 1611.507 kb on + strand at 1611.626 kb on + strand, within DZA65_RS07745 at 1611.687 kb on + strand at 1611.687 kb on + strand at 1611.727 kb on + strand at 1611.727 kb on + strand at 1611.728 kb on - strand at 1611.770 kb on + strand at 1611.770 kb on + strand at 1611.771 kb on - strand at 1611.771 kb on - strand at 1611.771 kb on - strand at 1611.771 kb on - strand at 1611.771 kb on - strand at 1611.994 kb on + strand at 1611.994 kb on + strand at 1611.994 kb on + strand at 1611.994 kb on + strand at 1611.994 kb on + strand at 1611.994 kb on + strand at 1611.994 kb on + strand at 1611.995 kb on - strand at 1611.995 kb on - strand at 1611.995 kb on - strand at 1611.995 kb on - strand at 1611.995 kb on - strand at 1612.021 kb on - strand at 1612.220 kb on + strand, within DZA65_RS07750 at 1612.220 kb on + strand, within DZA65_RS07750 at 1612.220 kb on + strand, within DZA65_RS07750 at 1612.221 kb on - strand, within DZA65_RS07750 at 1612.221 kb on - strand, within DZA65_RS07750 at 1612.295 kb on + strand, within DZA65_RS07750 at 1612.295 kb on + strand, within DZA65_RS07750 at 1612.295 kb on + strand, within DZA65_RS07750 at 1612.295 kb on + strand, within DZA65_RS07750 at 1612.296 kb on - strand, within DZA65_RS07750 at 1612.296 kb on - strand, within DZA65_RS07750 at 1612.296 kb on - strand, within DZA65_RS07750 at 1612.296 kb on - strand, within DZA65_RS07750 at 1612.301 kb on + strand, within DZA65_RS07750 at 1612.301 kb on + strand, within DZA65_RS07750 at 1612.301 kb on + strand, within DZA65_RS07750 at 1612.301 kb on + strand, within DZA65_RS07750 at 1612.301 kb on + strand, within DZA65_RS07750 at 1612.302 kb on - strand, within DZA65_RS07750 at 1612.302 kb on - strand, within DZA65_RS07750 at 1612.302 kb on - strand at 1612.333 kb on + strand, within DZA65_RS07750 at 1612.345 kb on + strand, within DZA65_RS07750 at 1612.346 kb on - strand, within DZA65_RS07750
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,609,121 + -1.2 1,609,168 + -0.2 1,609,168 + -1.6 1,609,168 + -0.2 1,609,168 + +0.4 1,609,168 + -0.1 1,609,169 - -0.1 1,609,169 - -0.2 1,609,205 + +1.3 1,609,205 + +1.3 1,609,206 - +0.5 1,609,206 - -0.8 1,609,206 - +0.2 1,609,206 - -0.2 1,609,223 + -1.3 1,609,223 + +0.5 1,609,223 + -1.8 1,609,223 + +0.5 1,609,223 + +0.0 1,609,224 - +1.9 1,609,224 - +0.2 1,609,224 - -0.1 1,609,268 + -0.6 1,609,269 - -0.2 1,609,296 + +0.5 1,609,457 + +0.4 1,609,457 + -0.1 1,609,458 - +0.2 1,609,542 + -0.5 1,609,542 + +0.9 1,609,543 - -2.9 1,609,889 - DZA65_RS07735 0.77 -0.5 1,609,889 - DZA65_RS07735 0.77 -1.8 1,610,017 + -2.6 1,610,017 + +0.1 1,610,017 + +1.8 1,610,018 - +0.2 1,610,018 - -2.3 1,610,018 - +0.1 1,610,042 - -0.1 1,610,067 + -0.8 1,610,067 + -0.3 1,610,067 + +0.2 1,610,067 + +0.5 1,610,067 + -0.5 1,610,068 - +0.4 1,610,068 - +0.5 1,610,068 - +0.9 1,610,068 - +0.3 1,610,068 - +0.2 1,610,068 - +0.3 1,610,068 - +1.9 1,610,068 - -0.8 1,610,068 - -0.5 1,610,068 - -0.7 1,610,068 - +1.5 1,610,068 - -0.5 1,610,126 - -0.1 1,610,166 + +0.5 1,610,178 + +0.3 1,610,178 + -0.3 1,610,194 + +0.3 1,610,202 + +1.4 1,610,202 + +0.9 1,610,203 - +0.1 1,610,244 + DZA65_RS07740 0.10 +0.4 1,610,254 + DZA65_RS07740 0.11 +0.3 1,610,255 - DZA65_RS07740 0.11 -1.3 1,610,263 - DZA65_RS07740 0.12 -0.5 1,610,263 - DZA65_RS07740 0.12 +0.7 1,610,263 - DZA65_RS07740 0.12 -0.6 1,610,400 + DZA65_RS07740 0.22 +0.1 1,610,400 + DZA65_RS07740 0.22 +1.1 1,610,401 - DZA65_RS07740 0.22 -1.3 1,610,449 + DZA65_RS07740 0.26 +1.5 1,610,449 + DZA65_RS07740 0.26 +1.6 1,610,450 - DZA65_RS07740 0.26 -0.1 1,610,451 + DZA65_RS07740 0.26 +0.0 1,610,452 - DZA65_RS07740 0.26 -0.3 1,610,452 - DZA65_RS07740 0.26 -0.7 1,610,458 + DZA65_RS07740 0.27 -0.7 1,610,459 - DZA65_RS07740 0.27 +1.8 1,610,474 + DZA65_RS07740 0.28 -0.5 1,610,475 - DZA65_RS07740 0.28 -0.1 1,610,475 - DZA65_RS07740 0.28 -0.5 1,610,505 - DZA65_RS07740 0.30 -2.3 1,610,505 - DZA65_RS07740 0.30 +0.5 1,610,505 - DZA65_RS07740 0.30 -1.2 1,610,518 + DZA65_RS07740 0.31 -0.2 1,610,549 - DZA65_RS07740 0.34 -0.2 1,610,742 + DZA65_RS07740 0.49 -2.1 1,610,742 + DZA65_RS07740 0.49 +0.5 1,610,742 + +0.5 1,610,742 + DZA65_RS07740 0.49 +1.5 1,610,743 - DZA65_RS07740 0.49 +0.8 1,610,743 - DZA65_RS07740 0.49 +0.0 1,610,743 - DZA65_RS07740 0.49 +0.3 1,610,743 - DZA65_RS07740 0.49 -0.1 1,610,743 - DZA65_RS07740 0.49 +0.6 1,610,756 - DZA65_RS07740 0.50 -0.5 1,610,855 + DZA65_RS07740 0.58 -0.7 1,610,856 - DZA65_RS07740 0.58 +2.4 1,610,856 - DZA65_RS07740 0.58 +0.3 1,610,877 + DZA65_RS07740 0.59 +1.1 1,611,095 + DZA65_RS07740 0.76 -0.1 1,611,095 + DZA65_RS07740 0.76 -0.2 1,611,096 - DZA65_RS07740 0.76 -1.1 1,611,096 - DZA65_RS07740 0.76 +0.9 1,611,096 - DZA65_RS07740 0.76 -0.2 1,611,163 + DZA65_RS07740 0.82 +0.4 1,611,164 - DZA65_RS07740 0.82 +0.7 1,611,164 - DZA65_RS07740 0.82 -0.6 1,611,221 + DZA65_RS07740 0.86 +0.4 1,611,221 + DZA65_RS07740 0.86 +1.8 1,611,221 + DZA65_RS07740 0.86 +0.8 1,611,221 + DZA65_RS07740 0.86 +1.4 1,611,221 + DZA65_RS07740 0.86 +1.4 1,611,221 + DZA65_RS07740 0.86 -0.6 1,611,221 + DZA65_RS07740 0.86 +0.3 1,611,222 - DZA65_RS07740 0.86 -0.2 1,611,231 + DZA65_RS07740 0.87 +1.3 1,611,231 + DZA65_RS07740 0.87 -0.9 1,611,235 + DZA65_RS07740 0.87 +0.4 1,611,235 + DZA65_RS07740 0.87 +0.4 1,611,240 - DZA65_RS07740 0.88 -1.1 1,611,269 - +1.5 1,611,269 - -0.4 1,611,274 - +0.8 1,611,274 - +0.5 1,611,274 - -0.3 1,611,318 + +2.6 1,611,318 + +0.4 1,611,318 + -0.8 1,611,365 + +1.4 1,611,366 - -0.1 1,611,366 - -0.5 1,611,389 - -2.1 1,611,507 + -0.5 1,611,626 + DZA65_RS07745 0.88 -1.9 1,611,687 + -0.1 1,611,687 + -0.7 1,611,727 + +0.1 1,611,727 + -0.1 1,611,728 - +0.5 1,611,770 + -0.2 1,611,770 + -0.5 1,611,771 - +0.3 1,611,771 - -0.1 1,611,771 - +1.0 1,611,771 - -0.8 1,611,771 - +0.8 1,611,994 + +0.7 1,611,994 + +0.7 1,611,994 + -0.4 1,611,994 + +0.0 1,611,994 + +0.5 1,611,994 + +0.8 1,611,994 + -0.8 1,611,995 - +0.0 1,611,995 - -0.3 1,611,995 - +0.8 1,611,995 - +1.2 1,611,995 - -0.1 1,612,021 - -1.3 1,612,220 + DZA65_RS07750 0.12 +1.2 1,612,220 + DZA65_RS07750 0.12 +0.3 1,612,220 + DZA65_RS07750 0.12 +1.6 1,612,221 - DZA65_RS07750 0.12 +0.9 1,612,221 - DZA65_RS07750 0.12 +0.5 1,612,295 + DZA65_RS07750 0.15 -0.5 1,612,295 + DZA65_RS07750 0.15 +0.5 1,612,295 + DZA65_RS07750 0.15 -1.1 1,612,295 + DZA65_RS07750 0.15 -1.1 1,612,296 - DZA65_RS07750 0.15 +0.7 1,612,296 - DZA65_RS07750 0.15 +0.1 1,612,296 - DZA65_RS07750 0.15 -1.1 1,612,296 - DZA65_RS07750 0.15 -0.2 1,612,301 + DZA65_RS07750 0.15 +0.9 1,612,301 + DZA65_RS07750 0.15 -0.2 1,612,301 + DZA65_RS07750 0.15 -1.8 1,612,301 + DZA65_RS07750 0.15 +0.5 1,612,301 + DZA65_RS07750 0.15 +0.5 1,612,302 - DZA65_RS07750 0.15 -0.2 1,612,302 - DZA65_RS07750 0.15 +0.5 1,612,302 - +0.5 1,612,333 + DZA65_RS07750 0.16 -1.2 1,612,345 + DZA65_RS07750 0.16 +0.8 1,612,346 - DZA65_RS07750 0.16 +0.9
Or see this region's nucleotide sequence