Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS05245 and DZA65_RS05250 are separated by 158 nucleotides DZA65_RS05250 and DZA65_RS05255 overlap by 1 nucleotides DZA65_RS05255 and DZA65_RS05260 are separated by 112 nucleotides
DZA65_RS05245: DZA65_RS05245 - A/G-specific adenine glycosylase, at 1,079,110 to 1,080,195
_RS05245
DZA65_RS05250: DZA65_RS05250 - tRNA (guanosine(46)-N7)-methyltransferase TrmB, at 1,080,354 to 1,081,073
_RS05250
DZA65_RS05255: DZA65_RS05255 - YggL family protein, at 1,081,073 to 1,081,417
_RS05255
DZA65_RS05260: DZA65_RS05260 - DUF2884 domain-containing protein, at 1,081,530 to 1,082,246
_RS05260
Position (kb)
1080
1081
1082 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1079.417 kb on + strand, within DZA65_RS05245 at 1079.418 kb on - strand, within DZA65_RS05245 at 1079.418 kb on - strand, within DZA65_RS05245 at 1079.418 kb on - strand, within DZA65_RS05245 at 1079.551 kb on - strand, within DZA65_RS05245 at 1079.551 kb on - strand, within DZA65_RS05245 at 1079.551 kb on - strand, within DZA65_RS05245 at 1079.551 kb on - strand, within DZA65_RS05245 at 1079.562 kb on - strand, within DZA65_RS05245 at 1079.713 kb on + strand, within DZA65_RS05245 at 1079.713 kb on + strand, within DZA65_RS05245 at 1079.713 kb on + strand, within DZA65_RS05245 at 1079.713 kb on + strand, within DZA65_RS05245 at 1079.714 kb on - strand, within DZA65_RS05245 at 1079.714 kb on - strand, within DZA65_RS05245 at 1079.820 kb on + strand, within DZA65_RS05245 at 1079.945 kb on + strand, within DZA65_RS05245 at 1079.945 kb on + strand, within DZA65_RS05245 at 1079.946 kb on - strand, within DZA65_RS05245 at 1079.946 kb on - strand, within DZA65_RS05245 at 1079.946 kb on - strand, within DZA65_RS05245 at 1079.949 kb on - strand, within DZA65_RS05245 at 1080.028 kb on - strand, within DZA65_RS05245 at 1080.041 kb on + strand, within DZA65_RS05245 at 1080.099 kb on + strand at 1080.099 kb on + strand at 1080.099 kb on + strand at 1080.100 kb on - strand at 1080.100 kb on - strand at 1080.100 kb on - strand at 1080.100 kb on - strand at 1080.101 kb on + strand at 1080.102 kb on - strand at 1080.102 kb on - strand at 1080.102 kb on - strand at 1080.102 kb on - strand at 1080.102 kb on - strand at 1080.102 kb on - strand at 1080.135 kb on + strand at 1080.135 kb on + strand at 1080.152 kb on + strand at 1080.152 kb on + strand at 1080.163 kb on + strand at 1080.195 kb on + strand at 1080.196 kb on - strand at 1080.254 kb on + strand at 1080.254 kb on + strand at 1080.254 kb on + strand at 1080.254 kb on + strand at 1080.254 kb on + strand at 1080.255 kb on - strand at 1080.255 kb on - strand at 1080.259 kb on + strand at 1080.259 kb on + strand at 1080.260 kb on - strand at 1080.284 kb on + strand at 1080.285 kb on - strand at 1080.291 kb on + strand at 1080.292 kb on - strand at 1080.292 kb on - strand at 1080.301 kb on + strand at 1080.302 kb on - strand at 1080.353 kb on + strand at 1080.353 kb on + strand at 1080.353 kb on + strand at 1080.353 kb on + strand at 1080.353 kb on + strand at 1080.353 kb on + strand at 1080.354 kb on - strand at 1080.354 kb on - strand at 1080.354 kb on - strand at 1080.354 kb on - strand at 1080.354 kb on - strand at 1080.362 kb on + strand at 1080.407 kb on + strand at 1080.408 kb on - strand at 1080.529 kb on + strand, within DZA65_RS05250 at 1080.630 kb on - strand, within DZA65_RS05250 at 1080.631 kb on + strand, within DZA65_RS05250 at 1080.631 kb on + strand, within DZA65_RS05250 at 1080.631 kb on + strand, within DZA65_RS05250 at 1080.631 kb on + strand, within DZA65_RS05250 at 1080.631 kb on + strand, within DZA65_RS05250 at 1080.632 kb on - strand, within DZA65_RS05250 at 1080.632 kb on - strand, within DZA65_RS05250 at 1080.632 kb on - strand, within DZA65_RS05250 at 1080.632 kb on - strand, within DZA65_RS05250 at 1080.632 kb on - strand, within DZA65_RS05250 at 1080.632 kb on - strand at 1080.632 kb on - strand, within DZA65_RS05250 at 1080.632 kb on - strand at 1080.809 kb on + strand, within DZA65_RS05250 at 1080.809 kb on + strand, within DZA65_RS05250 at 1080.810 kb on - strand, within DZA65_RS05250 at 1080.903 kb on + strand, within DZA65_RS05250 at 1080.903 kb on + strand, within DZA65_RS05250 at 1080.914 kb on + strand, within DZA65_RS05250 at 1080.914 kb on + strand, within DZA65_RS05250 at 1080.914 kb on + strand, within DZA65_RS05250 at 1080.914 kb on + strand, within DZA65_RS05250 at 1080.914 kb on + strand, within DZA65_RS05250 at 1080.915 kb on - strand, within DZA65_RS05250 at 1080.915 kb on - strand, within DZA65_RS05250 at 1080.915 kb on - strand, within DZA65_RS05250 at 1080.915 kb on - strand, within DZA65_RS05250 at 1080.915 kb on - strand, within DZA65_RS05250 at 1080.915 kb on - strand, within DZA65_RS05250 at 1080.915 kb on - strand, within DZA65_RS05250 at 1080.915 kb on - strand, within DZA65_RS05250 at 1080.915 kb on - strand, within DZA65_RS05250 at 1080.965 kb on + strand, within DZA65_RS05250 at 1080.965 kb on + strand, within DZA65_RS05250 at 1080.966 kb on - strand, within DZA65_RS05250 at 1080.966 kb on - strand, within DZA65_RS05250 at 1080.966 kb on - strand, within DZA65_RS05250 at 1080.966 kb on - strand, within DZA65_RS05250 at 1080.972 kb on + strand, within DZA65_RS05250 at 1080.972 kb on + strand, within DZA65_RS05250 at 1080.972 kb on + strand, within DZA65_RS05250 at 1080.972 kb on + strand, within DZA65_RS05250 at 1080.972 kb on + strand, within DZA65_RS05250 at 1080.973 kb on - strand, within DZA65_RS05250 at 1080.973 kb on - strand, within DZA65_RS05250 at 1080.973 kb on - strand, within DZA65_RS05250 at 1080.973 kb on - strand, within DZA65_RS05250 at 1081.072 kb on - strand at 1081.079 kb on - strand at 1081.099 kb on - strand at 1081.102 kb on + strand at 1081.103 kb on - strand at 1081.103 kb on - strand at 1081.260 kb on + strand, within DZA65_RS05255 at 1081.260 kb on + strand, within DZA65_RS05255 at 1081.261 kb on - strand, within DZA65_RS05255 at 1081.261 kb on - strand, within DZA65_RS05255 at 1081.261 kb on - strand, within DZA65_RS05255 at 1081.261 kb on - strand, within DZA65_RS05255 at 1081.330 kb on + strand, within DZA65_RS05255 at 1081.330 kb on + strand, within DZA65_RS05255 at 1081.330 kb on + strand, within DZA65_RS05255 at 1081.330 kb on + strand, within DZA65_RS05255 at 1081.331 kb on - strand, within DZA65_RS05255 at 1081.331 kb on - strand, within DZA65_RS05255 at 1081.331 kb on - strand, within DZA65_RS05255 at 1081.331 kb on - strand, within DZA65_RS05255 at 1081.331 kb on - strand, within DZA65_RS05255 at 1081.384 kb on + strand at 1081.384 kb on + strand at 1081.384 kb on + strand at 1081.445 kb on - strand at 1081.529 kb on + strand at 1081.529 kb on + strand at 1081.529 kb on + strand at 1081.529 kb on + strand at 1081.529 kb on + strand at 1081.529 kb on + strand at 1081.530 kb on - strand at 1081.530 kb on - strand at 1081.530 kb on - strand at 1081.530 kb on - strand at 1081.530 kb on - strand at 1081.538 kb on + strand at 1081.539 kb on - strand at 1081.539 kb on - strand at 1081.590 kb on + strand at 1081.590 kb on + strand at 1081.590 kb on + strand at 1081.693 kb on + strand, within DZA65_RS05260 at 1081.693 kb on + strand, within DZA65_RS05260 at 1081.693 kb on + strand, within DZA65_RS05260 at 1081.693 kb on + strand, within DZA65_RS05260 at 1081.693 kb on + strand, within DZA65_RS05260 at 1081.694 kb on - strand, within DZA65_RS05260 at 1081.694 kb on - strand, within DZA65_RS05260 at 1081.694 kb on - strand, within DZA65_RS05260 at 1081.694 kb on - strand, within DZA65_RS05260 at 1081.694 kb on - strand, within DZA65_RS05260 at 1081.694 kb on - strand, within DZA65_RS05260 at 1081.694 kb on - strand, within DZA65_RS05260 at 1081.694 kb on - strand, within DZA65_RS05260 at 1081.752 kb on + strand, within DZA65_RS05260 at 1081.753 kb on - strand, within DZA65_RS05260 at 1081.868 kb on + strand, within DZA65_RS05260 at 1081.869 kb on - strand, within DZA65_RS05260 at 1081.869 kb on - strand, within DZA65_RS05260 at 1081.869 kb on - strand, within DZA65_RS05260 at 1081.906 kb on + strand, within DZA65_RS05260 at 1081.907 kb on - strand, within DZA65_RS05260 at 1081.907 kb on - strand, within DZA65_RS05260 at 1081.928 kb on + strand, within DZA65_RS05260 at 1081.973 kb on + strand, within DZA65_RS05260
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,079,417 + DZA65_RS05245 0.28 -2.1 1,079,418 - DZA65_RS05245 0.28 -2.1 1,079,418 - DZA65_RS05245 0.28 +1.7 1,079,418 - DZA65_RS05245 0.28 +0.7 1,079,551 - DZA65_RS05245 0.41 -0.3 1,079,551 - DZA65_RS05245 0.41 -1.3 1,079,551 - DZA65_RS05245 0.41 +0.9 1,079,551 - DZA65_RS05245 0.41 +0.7 1,079,562 - DZA65_RS05245 0.42 -0.7 1,079,713 + DZA65_RS05245 0.56 -0.2 1,079,713 + DZA65_RS05245 0.56 -0.3 1,079,713 + DZA65_RS05245 0.56 +0.4 1,079,713 + DZA65_RS05245 0.56 -1.1 1,079,714 - DZA65_RS05245 0.56 +1.3 1,079,714 - DZA65_RS05245 0.56 +0.9 1,079,820 + DZA65_RS05245 0.65 -2.1 1,079,945 + DZA65_RS05245 0.77 +0.3 1,079,945 + DZA65_RS05245 0.77 -0.2 1,079,946 - DZA65_RS05245 0.77 -0.1 1,079,946 - DZA65_RS05245 0.77 -0.2 1,079,946 - DZA65_RS05245 0.77 +0.1 1,079,949 - DZA65_RS05245 0.77 +0.1 1,080,028 - DZA65_RS05245 0.85 -0.0 1,080,041 + DZA65_RS05245 0.86 +0.5 1,080,099 + -1.9 1,080,099 + -1.3 1,080,099 + -0.9 1,080,100 - +1.2 1,080,100 - +1.3 1,080,100 - -0.8 1,080,100 - +0.2 1,080,101 + -0.3 1,080,102 - -0.4 1,080,102 - -0.3 1,080,102 - +0.4 1,080,102 - -0.3 1,080,102 - +0.0 1,080,102 - -0.6 1,080,135 + +0.3 1,080,135 + -0.4 1,080,152 + -0.9 1,080,152 + -1.9 1,080,163 + -0.9 1,080,195 + +0.5 1,080,196 - -0.8 1,080,254 + -1.4 1,080,254 + -2.3 1,080,254 + -0.8 1,080,254 + -0.7 1,080,254 + -2.4 1,080,255 - -0.9 1,080,255 - +0.4 1,080,259 + -1.5 1,080,259 + +0.2 1,080,260 - +0.4 1,080,284 + -0.7 1,080,285 - -2.9 1,080,291 + +0.2 1,080,292 - +0.1 1,080,292 - +1.5 1,080,301 + -2.2 1,080,302 - +0.1 1,080,353 + -1.0 1,080,353 + -0.9 1,080,353 + +0.3 1,080,353 + -0.5 1,080,353 + +0.6 1,080,353 + -2.3 1,080,354 - +2.7 1,080,354 - -1.0 1,080,354 - -1.4 1,080,354 - +1.3 1,080,354 - -0.5 1,080,362 + -1.4 1,080,407 + -1.6 1,080,408 - -1.0 1,080,529 + DZA65_RS05250 0.24 +1.0 1,080,630 - DZA65_RS05250 0.38 -0.6 1,080,631 + DZA65_RS05250 0.38 -0.2 1,080,631 + DZA65_RS05250 0.38 -1.1 1,080,631 + DZA65_RS05250 0.38 +0.7 1,080,631 + DZA65_RS05250 0.38 +0.6 1,080,631 + DZA65_RS05250 0.38 +1.0 1,080,632 - DZA65_RS05250 0.39 -0.4 1,080,632 - DZA65_RS05250 0.39 -1.5 1,080,632 - DZA65_RS05250 0.39 -0.9 1,080,632 - DZA65_RS05250 0.39 +1.0 1,080,632 - DZA65_RS05250 0.39 +0.7 1,080,632 - -0.9 1,080,632 - DZA65_RS05250 0.39 -1.8 1,080,632 - -0.9 1,080,809 + DZA65_RS05250 0.63 +0.1 1,080,809 + DZA65_RS05250 0.63 +0.0 1,080,810 - DZA65_RS05250 0.63 +1.4 1,080,903 + DZA65_RS05250 0.76 -0.5 1,080,903 + DZA65_RS05250 0.76 +0.1 1,080,914 + DZA65_RS05250 0.78 -0.6 1,080,914 + DZA65_RS05250 0.78 +0.1 1,080,914 + DZA65_RS05250 0.78 +1.1 1,080,914 + DZA65_RS05250 0.78 +0.4 1,080,914 + DZA65_RS05250 0.78 -0.9 1,080,915 - DZA65_RS05250 0.78 +0.3 1,080,915 - DZA65_RS05250 0.78 +0.0 1,080,915 - DZA65_RS05250 0.78 -0.3 1,080,915 - DZA65_RS05250 0.78 +1.0 1,080,915 - DZA65_RS05250 0.78 -0.5 1,080,915 - DZA65_RS05250 0.78 -0.4 1,080,915 - DZA65_RS05250 0.78 +1.0 1,080,915 - DZA65_RS05250 0.78 -0.8 1,080,915 - DZA65_RS05250 0.78 -0.4 1,080,965 + DZA65_RS05250 0.85 -0.3 1,080,965 + DZA65_RS05250 0.85 -0.1 1,080,966 - DZA65_RS05250 0.85 -0.7 1,080,966 - DZA65_RS05250 0.85 -0.0 1,080,966 - DZA65_RS05250 0.85 -0.4 1,080,966 - DZA65_RS05250 0.85 -0.9 1,080,972 + DZA65_RS05250 0.86 -0.2 1,080,972 + DZA65_RS05250 0.86 -2.7 1,080,972 + DZA65_RS05250 0.86 +0.1 1,080,972 + DZA65_RS05250 0.86 -0.4 1,080,972 + DZA65_RS05250 0.86 +0.5 1,080,973 - DZA65_RS05250 0.86 -0.3 1,080,973 - DZA65_RS05250 0.86 -0.3 1,080,973 - DZA65_RS05250 0.86 -0.9 1,080,973 - DZA65_RS05250 0.86 -0.1 1,081,072 - -1.6 1,081,079 - -0.3 1,081,099 - -1.9 1,081,102 + +0.2 1,081,103 - -1.0 1,081,103 - -3.3 1,081,260 + DZA65_RS05255 0.54 -2.1 1,081,260 + DZA65_RS05255 0.54 -1.1 1,081,261 - DZA65_RS05255 0.54 -0.7 1,081,261 - DZA65_RS05255 0.54 -0.7 1,081,261 - DZA65_RS05255 0.54 -0.6 1,081,261 - DZA65_RS05255 0.54 +0.2 1,081,330 + DZA65_RS05255 0.74 -0.3 1,081,330 + DZA65_RS05255 0.74 +0.4 1,081,330 + DZA65_RS05255 0.74 -0.6 1,081,330 + DZA65_RS05255 0.74 -1.1 1,081,331 - DZA65_RS05255 0.75 +0.1 1,081,331 - DZA65_RS05255 0.75 -0.1 1,081,331 - DZA65_RS05255 0.75 +1.1 1,081,331 - DZA65_RS05255 0.75 +0.4 1,081,331 - DZA65_RS05255 0.75 -0.8 1,081,384 + +0.5 1,081,384 + +0.8 1,081,384 + -1.6 1,081,445 - -0.1 1,081,529 + +0.1 1,081,529 + -2.6 1,081,529 + -0.1 1,081,529 + -0.1 1,081,529 + -0.4 1,081,529 + -0.1 1,081,530 - -0.6 1,081,530 - -0.1 1,081,530 - +1.2 1,081,530 - +1.9 1,081,530 - +0.5 1,081,538 + -1.9 1,081,539 - -1.9 1,081,539 - -0.6 1,081,590 + -1.5 1,081,590 + +0.5 1,081,590 + -0.4 1,081,693 + DZA65_RS05260 0.23 +0.4 1,081,693 + DZA65_RS05260 0.23 -2.5 1,081,693 + DZA65_RS05260 0.23 -0.3 1,081,693 + DZA65_RS05260 0.23 -2.5 1,081,693 + DZA65_RS05260 0.23 -0.9 1,081,694 - DZA65_RS05260 0.23 -0.1 1,081,694 - DZA65_RS05260 0.23 -0.6 1,081,694 - DZA65_RS05260 0.23 +0.5 1,081,694 - DZA65_RS05260 0.23 -0.0 1,081,694 - DZA65_RS05260 0.23 +1.3 1,081,694 - DZA65_RS05260 0.23 -0.8 1,081,694 - DZA65_RS05260 0.23 +0.3 1,081,694 - DZA65_RS05260 0.23 +0.0 1,081,752 + DZA65_RS05260 0.31 -1.2 1,081,753 - DZA65_RS05260 0.31 -0.7 1,081,868 + DZA65_RS05260 0.47 +0.4 1,081,869 - DZA65_RS05260 0.47 -0.4 1,081,869 - DZA65_RS05260 0.47 -0.9 1,081,869 - DZA65_RS05260 0.47 -0.9 1,081,906 + DZA65_RS05260 0.52 -0.1 1,081,907 - DZA65_RS05260 0.53 +0.3 1,081,907 - DZA65_RS05260 0.53 -0.5 1,081,928 + DZA65_RS05260 0.56 -0.6 1,081,973 + DZA65_RS05260 0.62 -0.0
Or see this region's nucleotide sequence