Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS03195 and DZA65_RS03200 are separated by 105 nucleotides DZA65_RS03200 and DZA65_RS03205 are separated by 8 nucleotides DZA65_RS03205 and DZA65_RS03210 are separated by 298 nucleotides DZA65_RS03210 and DZA65_RS03215 are separated by 54 nucleotides DZA65_RS03215 and DZA65_RS03220 are separated by 59 nucleotides
DZA65_RS03195: DZA65_RS03195 - ATP-dependent zinc metalloprotease FtsH, at 677,470 to 679,422
_RS03195
DZA65_RS03200: DZA65_RS03200 - dihydropteroate synthase, at 679,528 to 680,361
_RS03200
DZA65_RS03205: DZA65_RS03205 - phosphoglucosamine mutase, at 680,370 to 681,707
_RS03205
DZA65_RS03210: DZA65_RS03210 - preprotein translocase subunit SecG, at 682,006 to 682,341
_RS03210
DZA65_RS03215: DZA65_RS03215 - tRNA-Leu, at 682,396 to 682,482
_RS03215
DZA65_RS03220: DZA65_RS03220 - tRNA-Met, at 682,542 to 682,618
_RS03220
Position (kb) 680
681
682 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 679.389 kb on + strand at 679.389 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.393 kb on - strand at 679.393 kb on - strand at 679.393 kb on - strand at 679.393 kb on - strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.448 kb on + strand at 679.448 kb on + strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.484 kb on - strand at 679.484 kb on - strand at 679.515 kb on + strand at 679.516 kb on - strand at 679.516 kb on - strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.325 kb on - strand at 680.325 kb on - strand at 680.325 kb on - strand at 680.330 kb on + strand at 680.330 kb on + strand at 680.330 kb on + strand at 680.346 kb on - strand at 681.706 kb on - strand at 681.717 kb on + strand at 681.717 kb on + strand at 681.717 kb on + strand at 681.717 kb on + strand at 681.718 kb on - strand at 681.718 kb on - strand at 681.766 kb on + strand at 681.782 kb on + strand at 681.783 kb on - strand at 681.795 kb on + strand at 681.863 kb on - strand at 681.886 kb on + strand at 681.919 kb on + strand at 681.919 kb on + strand at 681.919 kb on + strand at 681.919 kb on + strand at 681.919 kb on + strand at 681.961 kb on + strand at 681.988 kb on + strand at 681.999 kb on + strand at 682.062 kb on + strand, within DZA65_RS03210 at 682.062 kb on + strand, within DZA65_RS03210 at 682.080 kb on + strand, within DZA65_RS03210 at 682.080 kb on + strand, within DZA65_RS03210 at 682.335 kb on + strand at 682.339 kb on + strand at 682.380 kb on + strand at 682.487 kb on + strand at 682.488 kb on - strand at 682.488 kb on - strand at 682.488 kb on - strand at 682.488 kb on - strand at 682.488 kb on - strand at 682.490 kb on - strand at 682.493 kb on + strand at 682.493 kb on + strand at 682.493 kb on + strand at 682.493 kb on + strand at 682.493 kb on + strand at 682.493 kb on + strand at 682.493 kb on + strand at 682.493 kb on + strand at 682.493 kb on + strand at 682.493 kb on + strand at 682.493 kb on + strand at 682.494 kb on - strand at 682.494 kb on - strand at 682.494 kb on - strand at 682.621 kb on + strand at 682.656 kb on + strand at 682.656 kb on + strand at 682.707 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 679,389 + +0.2 679,389 + -1.6 679,392 + -1.4 679,392 + -1.0 679,392 + -0.4 679,392 + -0.7 679,392 + +1.3 679,393 - +2.7 679,393 - -1.7 679,393 - -0.4 679,393 - -0.4 679,420 + +0.5 679,420 + -0.2 679,420 + -0.0 679,420 + -0.7 679,420 + +0.0 679,420 + +0.5 679,420 + -0.6 679,421 - +0.6 679,421 - -0.7 679,421 - +0.5 679,421 - -1.5 679,421 - +0.6 679,421 - -2.6 679,421 - +0.6 679,421 - -0.4 679,421 - -2.1 679,421 - -0.4 679,427 - +0.0 679,427 - -0.4 679,427 - -2.5 679,427 - -0.7 679,427 - +0.1 679,427 - -0.5 679,448 + -1.4 679,448 + -0.4 679,449 - -2.1 679,449 - +1.2 679,449 - -2.0 679,449 - -0.9 679,449 - -2.0 679,449 - -0.4 679,449 - -1.0 679,449 - +1.2 679,449 - +0.2 679,484 - -1.4 679,484 - -1.7 679,515 + -1.4 679,516 - -0.2 679,516 - -0.4 680,324 + -0.1 680,324 + -1.4 680,324 + -1.9 680,324 + -1.4 680,324 + -0.7 680,324 + -2.3 680,324 + -2.0 680,324 + -1.1 680,324 + -1.4 680,325 - -4.2 680,325 - -1.6 680,325 - -0.7 680,330 + +0.5 680,330 + -1.4 680,330 + -0.6 680,346 - -1.9 681,706 - +0.9 681,717 + -0.1 681,717 + -0.2 681,717 + -1.3 681,717 + -0.1 681,718 - -0.7 681,718 - -1.6 681,766 + +0.4 681,782 + +0.2 681,783 - -0.7 681,795 + -1.2 681,863 - -0.3 681,886 + -1.4 681,919 + -0.4 681,919 + +0.3 681,919 + -0.4 681,919 + +0.7 681,919 + +0.0 681,961 + +0.5 681,988 + +0.9 681,999 + +0.6 682,062 + DZA65_RS03210 0.17 -0.9 682,062 + DZA65_RS03210 0.17 -0.4 682,080 + DZA65_RS03210 0.22 +1.3 682,080 + DZA65_RS03210 0.22 -1.1 682,335 + -0.4 682,339 + -0.1 682,380 + +0.3 682,487 + +1.3 682,488 - +0.9 682,488 - +1.6 682,488 - +0.1 682,488 - +0.5 682,488 - -0.0 682,490 - +0.6 682,493 + -0.7 682,493 + +1.5 682,493 + +0.6 682,493 + -0.7 682,493 + -0.7 682,493 + +0.4 682,493 + -0.3 682,493 + -1.1 682,493 + -1.1 682,493 + -0.3 682,493 + -0.1 682,494 - -2.3 682,494 - +0.2 682,494 - -0.6 682,621 + -0.4 682,656 + -0.1 682,656 + +1.0 682,707 + +0.3
Or see this region's nucleotide sequence