Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS02280 and DZA65_RS02290 are separated by 133 nucleotides DZA65_RS02290 and DZA65_RS02295 are separated by 81 nucleotides DZA65_RS02295 and DZA65_RS02300 are separated by 66 nucleotides
DZA65_RS02280: DZA65_RS02280 - FGGY-family carbohydrate kinase, at 494,677 to 496,188
_RS02280
DZA65_RS02290: DZA65_RS02290 - SDR family oxidoreductase, at 496,322 to 496,396
_RS02290
DZA65_RS02295: DZA65_RS02295 - cytolytic delta-endotoxin, at 496,478 to 497,140
_RS02295
DZA65_RS02300: DZA65_RS02300 - type-1Ba cytolytic delta-endotoxin, at 497,207 to 497,818
_RS02300
Position (kb) 496
497 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 495.422 kb on - strand, within DZA65_RS02280 at 495.463 kb on + strand, within DZA65_RS02280 at 495.463 kb on + strand, within DZA65_RS02280 at 495.464 kb on - strand, within DZA65_RS02280 at 495.464 kb on - strand, within DZA65_RS02280 at 495.565 kb on + strand, within DZA65_RS02280 at 495.566 kb on - strand, within DZA65_RS02280 at 495.606 kb on - strand, within DZA65_RS02280 at 495.617 kb on + strand, within DZA65_RS02280 at 495.618 kb on - strand, within DZA65_RS02280 at 495.634 kb on - strand, within DZA65_RS02280 at 495.709 kb on + strand, within DZA65_RS02280 at 495.709 kb on + strand, within DZA65_RS02280 at 495.709 kb on + strand, within DZA65_RS02280 at 495.709 kb on + strand, within DZA65_RS02280 at 495.709 kb on + strand, within DZA65_RS02280 at 495.709 kb on + strand, within DZA65_RS02280 at 495.709 kb on + strand, within DZA65_RS02280 at 495.709 kb on + strand, within DZA65_RS02280 at 495.710 kb on - strand, within DZA65_RS02280 at 495.710 kb on - strand, within DZA65_RS02280 at 495.710 kb on - strand, within DZA65_RS02280 at 495.710 kb on - strand, within DZA65_RS02280 at 495.845 kb on + strand, within DZA65_RS02280 at 495.905 kb on + strand, within DZA65_RS02280 at 495.905 kb on + strand, within DZA65_RS02280 at 495.905 kb on + strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 495.906 kb on - strand, within DZA65_RS02280 at 496.067 kb on + strand at 496.083 kb on + strand at 496.084 kb on - strand at 496.084 kb on - strand at 496.084 kb on - strand at 496.152 kb on - strand at 496.234 kb on + strand at 496.297 kb on - strand at 496.346 kb on + strand, within DZA65_RS02290 at 496.346 kb on + strand, within DZA65_RS02290 at 496.346 kb on + strand, within DZA65_RS02290 at 496.346 kb on + strand, within DZA65_RS02290 at 496.347 kb on - strand, within DZA65_RS02290 at 496.347 kb on - strand, within DZA65_RS02290 at 496.347 kb on - strand, within DZA65_RS02290 at 496.347 kb on - strand, within DZA65_RS02290 at 496.408 kb on + strand at 496.408 kb on + strand at 496.408 kb on + strand at 496.408 kb on + strand at 496.409 kb on - strand at 496.409 kb on - strand at 496.409 kb on - strand at 496.409 kb on - strand at 496.409 kb on - strand at 496.411 kb on + strand at 496.411 kb on + strand at 496.412 kb on - strand at 496.417 kb on + strand at 496.417 kb on + strand at 496.417 kb on + strand at 496.417 kb on + strand at 496.418 kb on - strand at 496.418 kb on - strand at 496.418 kb on - strand at 496.418 kb on - strand at 496.418 kb on - strand at 496.418 kb on - strand at 496.418 kb on - strand at 496.418 kb on - strand at 496.418 kb on - strand at 496.476 kb on + strand at 496.476 kb on + strand at 496.477 kb on - strand at 496.487 kb on + strand at 496.505 kb on + strand at 496.520 kb on + strand at 496.527 kb on + strand at 496.528 kb on - strand at 496.528 kb on - strand at 496.553 kb on - strand, within DZA65_RS02295 at 496.553 kb on - strand, within DZA65_RS02295 at 496.640 kb on + strand, within DZA65_RS02295 at 496.640 kb on + strand, within DZA65_RS02295 at 496.640 kb on + strand, within DZA65_RS02295 at 496.641 kb on - strand, within DZA65_RS02295 at 496.641 kb on - strand, within DZA65_RS02295 at 496.650 kb on + strand, within DZA65_RS02295 at 496.650 kb on + strand, within DZA65_RS02295 at 496.651 kb on - strand, within DZA65_RS02295 at 496.654 kb on + strand, within DZA65_RS02295 at 496.654 kb on + strand, within DZA65_RS02295 at 496.654 kb on + strand, within DZA65_RS02295 at 496.654 kb on + strand, within DZA65_RS02295 at 496.655 kb on - strand, within DZA65_RS02295 at 496.655 kb on - strand, within DZA65_RS02295 at 496.655 kb on - strand, within DZA65_RS02295 at 496.655 kb on - strand, within DZA65_RS02295 at 496.655 kb on - strand at 496.656 kb on + strand, within DZA65_RS02295 at 496.656 kb on + strand, within DZA65_RS02295 at 496.656 kb on + strand, within DZA65_RS02295 at 496.656 kb on + strand, within DZA65_RS02295 at 496.656 kb on + strand, within DZA65_RS02295 at 496.656 kb on + strand, within DZA65_RS02295 at 496.656 kb on + strand, within DZA65_RS02295 at 496.656 kb on + strand, within DZA65_RS02295 at 496.656 kb on + strand, within DZA65_RS02295 at 496.656 kb on + strand, within DZA65_RS02295 at 496.656 kb on + strand, within DZA65_RS02295 at 496.657 kb on - strand, within DZA65_RS02295 at 496.657 kb on - strand, within DZA65_RS02295 at 496.657 kb on - strand, within DZA65_RS02295 at 496.657 kb on - strand, within DZA65_RS02295 at 496.657 kb on - strand, within DZA65_RS02295 at 496.657 kb on - strand, within DZA65_RS02295 at 496.669 kb on + strand, within DZA65_RS02295 at 496.725 kb on - strand, within DZA65_RS02295 at 496.757 kb on - strand, within DZA65_RS02295 at 496.812 kb on - strand, within DZA65_RS02295 at 496.877 kb on + strand, within DZA65_RS02295 at 496.878 kb on - strand, within DZA65_RS02295 at 496.916 kb on - strand, within DZA65_RS02295 at 496.930 kb on + strand, within DZA65_RS02295 at 496.931 kb on - strand, within DZA65_RS02295 at 496.931 kb on - strand, within DZA65_RS02295 at 496.931 kb on - strand, within DZA65_RS02295 at 496.981 kb on + strand, within DZA65_RS02295 at 496.981 kb on + strand, within DZA65_RS02295 at 496.981 kb on + strand, within DZA65_RS02295 at 496.981 kb on + strand, within DZA65_RS02295 at 496.982 kb on - strand, within DZA65_RS02295 at 496.982 kb on - strand, within DZA65_RS02295 at 496.983 kb on + strand, within DZA65_RS02295 at 496.984 kb on - strand, within DZA65_RS02295 at 496.984 kb on - strand, within DZA65_RS02295 at 496.984 kb on - strand, within DZA65_RS02295 at 496.984 kb on - strand, within DZA65_RS02295 at 497.042 kb on + strand, within DZA65_RS02295 at 497.049 kb on + strand, within DZA65_RS02295 at 497.077 kb on + strand at 497.103 kb on + strand at 497.103 kb on + strand at 497.103 kb on + strand at 497.103 kb on + strand at 497.103 kb on + strand at 497.103 kb on + strand at 497.104 kb on - strand at 497.104 kb on - strand at 497.104 kb on - strand at 497.104 kb on - strand at 497.104 kb on - strand at 497.107 kb on + strand at 497.107 kb on + strand at 497.107 kb on + strand at 497.107 kb on + strand at 497.108 kb on - strand at 497.108 kb on - strand at 497.131 kb on + strand at 497.131 kb on + strand at 497.132 kb on - strand at 497.140 kb on + strand at 497.140 kb on + strand at 497.141 kb on - strand at 497.141 kb on - strand at 497.141 kb on - strand at 497.141 kb on - strand at 497.141 kb on - strand at 497.159 kb on + strand at 497.160 kb on - strand at 497.191 kb on - strand at 497.195 kb on + strand at 497.297 kb on + strand, within DZA65_RS02300 at 497.391 kb on - strand, within DZA65_RS02300
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 495,422 - DZA65_RS02280 0.49 -0.3 495,463 + DZA65_RS02280 0.52 +1.0 495,463 + DZA65_RS02280 0.52 -0.6 495,464 - DZA65_RS02280 0.52 +1.2 495,464 - DZA65_RS02280 0.52 -0.0 495,565 + DZA65_RS02280 0.59 +0.6 495,566 - DZA65_RS02280 0.59 +0.3 495,606 - DZA65_RS02280 0.61 -0.7 495,617 + DZA65_RS02280 0.62 -0.4 495,618 - DZA65_RS02280 0.62 +1.2 495,634 - DZA65_RS02280 0.63 -0.3 495,709 + DZA65_RS02280 0.68 +0.3 495,709 + DZA65_RS02280 0.68 -0.5 495,709 + DZA65_RS02280 0.68 -0.2 495,709 + DZA65_RS02280 0.68 -0.2 495,709 + DZA65_RS02280 0.68 -0.4 495,709 + DZA65_RS02280 0.68 -0.9 495,709 + DZA65_RS02280 0.68 +0.1 495,709 + DZA65_RS02280 0.68 +1.1 495,710 - DZA65_RS02280 0.68 -0.4 495,710 - DZA65_RS02280 0.68 -1.0 495,710 - DZA65_RS02280 0.68 +0.4 495,710 - DZA65_RS02280 0.68 +1.6 495,845 + DZA65_RS02280 0.77 -0.8 495,905 + DZA65_RS02280 0.81 -1.7 495,905 + DZA65_RS02280 0.81 +0.7 495,905 + DZA65_RS02280 0.81 +0.8 495,906 - DZA65_RS02280 0.81 +0.3 495,906 - DZA65_RS02280 0.81 -2.2 495,906 - DZA65_RS02280 0.81 -0.6 495,906 - DZA65_RS02280 0.81 +0.2 495,906 - DZA65_RS02280 0.81 -1.0 495,906 - DZA65_RS02280 0.81 -1.6 495,906 - DZA65_RS02280 0.81 -0.7 495,906 - DZA65_RS02280 0.81 -0.1 495,906 - DZA65_RS02280 0.81 -1.0 495,906 - DZA65_RS02280 0.81 -0.0 495,906 - DZA65_RS02280 0.81 -0.4 496,067 + +0.4 496,083 + -1.1 496,084 - -1.0 496,084 - +0.3 496,084 - -1.0 496,152 - +0.7 496,234 + -0.3 496,297 - +0.8 496,346 + DZA65_RS02290 0.32 +1.6 496,346 + DZA65_RS02290 0.32 -0.0 496,346 + DZA65_RS02290 0.32 -1.0 496,346 + DZA65_RS02290 0.32 +0.8 496,347 - DZA65_RS02290 0.33 +0.7 496,347 - DZA65_RS02290 0.33 -1.1 496,347 - DZA65_RS02290 0.33 +0.2 496,347 - DZA65_RS02290 0.33 +0.1 496,408 + +0.4 496,408 + +1.0 496,408 + +0.6 496,408 + +0.1 496,409 - +0.6 496,409 - -0.0 496,409 - -0.6 496,409 - +0.7 496,409 - -1.8 496,411 + +3.6 496,411 + -0.1 496,412 - +0.6 496,417 + +0.3 496,417 + +0.4 496,417 + +0.4 496,417 + +0.1 496,418 - -0.7 496,418 - -1.3 496,418 - -2.1 496,418 - +1.7 496,418 - +0.4 496,418 - +0.4 496,418 - +0.2 496,418 - +2.0 496,418 - +0.1 496,476 + -2.6 496,476 + -0.8 496,477 - -0.4 496,487 + +0.3 496,505 + -0.0 496,520 + +0.1 496,527 + +0.6 496,528 - +0.4 496,528 - +0.3 496,553 - DZA65_RS02295 0.11 +1.7 496,553 - DZA65_RS02295 0.11 +2.2 496,640 + DZA65_RS02295 0.24 -0.6 496,640 + DZA65_RS02295 0.24 +2.0 496,640 + DZA65_RS02295 0.24 -1.4 496,641 - DZA65_RS02295 0.25 +0.2 496,641 - DZA65_RS02295 0.25 -2.4 496,650 + DZA65_RS02295 0.26 -0.9 496,650 + DZA65_RS02295 0.26 -1.0 496,651 - DZA65_RS02295 0.26 +0.3 496,654 + DZA65_RS02295 0.27 -1.0 496,654 + DZA65_RS02295 0.27 +0.0 496,654 + DZA65_RS02295 0.27 -0.2 496,654 + DZA65_RS02295 0.27 -0.6 496,655 - DZA65_RS02295 0.27 -0.4 496,655 - DZA65_RS02295 0.27 -0.5 496,655 - DZA65_RS02295 0.27 -0.4 496,655 - DZA65_RS02295 0.27 -0.5 496,655 - +2.0 496,656 + DZA65_RS02295 0.27 -0.4 496,656 + DZA65_RS02295 0.27 -0.4 496,656 + DZA65_RS02295 0.27 -0.1 496,656 + DZA65_RS02295 0.27 +0.4 496,656 + DZA65_RS02295 0.27 +2.0 496,656 + DZA65_RS02295 0.27 -0.6 496,656 + DZA65_RS02295 0.27 -1.1 496,656 + DZA65_RS02295 0.27 -1.4 496,656 + DZA65_RS02295 0.27 +0.7 496,656 + DZA65_RS02295 0.27 -0.8 496,656 + DZA65_RS02295 0.27 +0.5 496,657 - DZA65_RS02295 0.27 +0.4 496,657 - DZA65_RS02295 0.27 +0.3 496,657 - DZA65_RS02295 0.27 +1.1 496,657 - DZA65_RS02295 0.27 +0.6 496,657 - DZA65_RS02295 0.27 -0.5 496,657 - DZA65_RS02295 0.27 -1.0 496,669 + DZA65_RS02295 0.29 -1.0 496,725 - DZA65_RS02295 0.37 -0.4 496,757 - DZA65_RS02295 0.42 -0.8 496,812 - DZA65_RS02295 0.50 +1.0 496,877 + DZA65_RS02295 0.60 -0.0 496,878 - DZA65_RS02295 0.60 +1.0 496,916 - DZA65_RS02295 0.66 +0.6 496,930 + DZA65_RS02295 0.68 -0.1 496,931 - DZA65_RS02295 0.68 +0.4 496,931 - DZA65_RS02295 0.68 +0.9 496,931 - DZA65_RS02295 0.68 -0.6 496,981 + DZA65_RS02295 0.76 +0.6 496,981 + DZA65_RS02295 0.76 +0.5 496,981 + DZA65_RS02295 0.76 +0.2 496,981 + DZA65_RS02295 0.76 +1.8 496,982 - DZA65_RS02295 0.76 -0.4 496,982 - DZA65_RS02295 0.76 +0.5 496,983 + DZA65_RS02295 0.76 +1.2 496,984 - DZA65_RS02295 0.76 -0.3 496,984 - DZA65_RS02295 0.76 +1.0 496,984 - DZA65_RS02295 0.76 -0.0 496,984 - DZA65_RS02295 0.76 +0.2 497,042 + DZA65_RS02295 0.85 +0.6 497,049 + DZA65_RS02295 0.86 -2.2 497,077 + +0.4 497,103 + -0.8 497,103 + +1.3 497,103 + -1.8 497,103 + +1.1 497,103 + -0.6 497,103 + -2.4 497,104 - -0.4 497,104 - +0.3 497,104 - +0.3 497,104 - +0.7 497,104 - -0.3 497,107 + +1.0 497,107 + +0.3 497,107 + -1.6 497,107 + +0.0 497,108 - -0.4 497,108 - +0.5 497,131 + -0.1 497,131 + -0.2 497,132 - -0.8 497,140 + +0.3 497,140 + -0.8 497,141 - -1.9 497,141 - -0.2 497,141 - +0.7 497,141 - +0.2 497,141 - -0.4 497,159 + +0.5 497,160 - -0.6 497,191 - -0.4 497,195 + +0.3 497,297 + DZA65_RS02300 0.15 +0.4 497,391 - DZA65_RS02300 0.30 -1.6
Or see this region's nucleotide sequence