Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01075 and DZA65_RS01080 are separated by 102 nucleotides DZA65_RS01080 and DZA65_RS01085 are separated by 277 nucleotides DZA65_RS01085 and DZA65_RS01090 overlap by 4 nucleotides DZA65_RS01090 and DZA65_RS01095 are separated by 26 nucleotides
DZA65_RS01075: DZA65_RS01075 - 23S ribosomal RNA, at 242,281 to 245,304
_RS01075
DZA65_RS01080: DZA65_RS01080 - 5S ribosomal RNA, at 245,407 to 245,522
_RS01080
DZA65_RS01085: DZA65_RS01085 - UDP-N-acetylmuramate dehydrogenase, at 245,800 to 246,837
_RS01085
DZA65_RS01090: DZA65_RS01090 - bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA, at 246,834 to 247,793
_RS01090
DZA65_RS01095: DZA65_RS01095 - type I pantothenate kinase, at 247,820 to 248,770
_RS01095
Position (kb) 245
246
247 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 245.684 kb on + strand at 245.684 kb on + strand at 245.685 kb on - strand at 245.685 kb on - strand at 245.763 kb on + strand at 245.763 kb on + strand at 245.768 kb on + strand at 245.768 kb on + strand at 245.768 kb on + strand at 245.768 kb on + strand at 245.768 kb on + strand at 245.768 kb on + strand at 246.094 kb on - strand, within DZA65_RS01085 at 247.792 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 245,684 + -1.5 245,684 + +0.7 245,685 - -1.8 245,685 - -1.8 245,763 + +1.9 245,763 + -1.0 245,768 + -0.5 245,768 + +1.2 245,768 + -0.5 245,768 + -1.5 245,768 + +0.7 245,768 + -1.4 246,094 - DZA65_RS01085 0.28 +2.8 247,792 - -0.8
Or see this region's nucleotide sequence