Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS18125 and DDA3937_RS18130 are separated by 12 nucleotides DDA3937_RS18130 and DDA3937_RS18135 are separated by 167 nucleotides DDA3937_RS18135 and DDA3937_RS22460 are separated by 247 nucleotides DDA3937_RS22460 and DDA3937_RS18140 are separated by 96 nucleotides
DDA3937_RS18125: DDA3937_RS18125 - 3-isopropylmalate dehydratase large subunit, at 4,138,397 to 4,139,797
_RS18125
DDA3937_RS18130: DDA3937_RS18130 - 3-isopropylmalate dehydratase small subunit, at 4,139,810 to 4,140,412
_RS18130
DDA3937_RS18135: DDA3937_RS18135 - siderophore-interacting protein, at 4,140,580 to 4,141,479
_RS18135
DDA3937_RS22460: DDA3937_RS22460 - tryptophanase leader peptide, at 4,141,727 to 4,141,837
_RS22460
DDA3937_RS18140: DDA3937_RS18140 - tryptophanase, at 4,141,934 to 4,143,334
_RS18140
Position (kb)
4140
4141
4142 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4139.686 kb on + strand at 4139.686 kb on + strand at 4139.687 kb on - strand at 4139.761 kb on + strand at 4139.796 kb on - strand at 4139.796 kb on - strand at 4139.796 kb on - strand at 4139.796 kb on - strand at 4139.830 kb on + strand at 4139.830 kb on + strand at 4139.830 kb on + strand at 4139.830 kb on + strand at 4139.830 kb on + strand at 4139.831 kb on - strand at 4140.009 kb on - strand, within DDA3937_RS18130 at 4140.138 kb on - strand, within DDA3937_RS18130 at 4140.284 kb on + strand, within DDA3937_RS18130 at 4140.285 kb on - strand, within DDA3937_RS18130 at 4140.298 kb on - strand, within DDA3937_RS18130 at 4140.380 kb on + strand at 4140.444 kb on + strand at 4140.494 kb on + strand at 4140.494 kb on + strand at 4140.494 kb on + strand at 4140.495 kb on - strand at 4140.495 kb on - strand at 4140.495 kb on - strand at 4140.495 kb on - strand at 4140.536 kb on - strand at 4140.845 kb on + strand, within DDA3937_RS18135 at 4140.931 kb on + strand at 4140.996 kb on + strand, within DDA3937_RS18135 at 4140.996 kb on + strand, within DDA3937_RS18135 at 4140.997 kb on - strand, within DDA3937_RS18135 at 4141.227 kb on + strand, within DDA3937_RS18135 at 4141.227 kb on + strand, within DDA3937_RS18135 at 4141.229 kb on + strand, within DDA3937_RS18135 at 4141.229 kb on + strand, within DDA3937_RS18135 at 4141.278 kb on + strand at 4141.278 kb on + strand, within DDA3937_RS18135 at 4141.280 kb on + strand, within DDA3937_RS18135 at 4141.280 kb on + strand, within DDA3937_RS18135 at 4141.281 kb on - strand, within DDA3937_RS18135 at 4141.397 kb on - strand at 4141.444 kb on - strand at 4141.458 kb on + strand at 4141.458 kb on + strand at 4141.459 kb on - strand at 4141.491 kb on - strand at 4141.491 kb on - strand at 4141.491 kb on - strand at 4141.533 kb on + strand at 4141.534 kb on - strand at 4141.534 kb on - strand at 4141.534 kb on - strand at 4141.534 kb on - strand at 4141.616 kb on - strand at 4141.689 kb on + strand at 4141.694 kb on + strand at 4141.699 kb on - strand at 4141.705 kb on - strand at 4141.715 kb on + strand at 4141.865 kb on + strand at 4142.030 kb on + strand at 4142.030 kb on + strand at 4142.030 kb on + strand at 4142.030 kb on + strand at 4142.030 kb on + strand at 4142.031 kb on - strand at 4142.031 kb on - strand at 4142.063 kb on + strand at 4142.063 kb on + strand at 4142.063 kb on + strand at 4142.064 kb on - strand at 4142.064 kb on - strand at 4142.064 kb on - strand at 4142.064 kb on - strand at 4142.145 kb on - strand, within DDA3937_RS18140 at 4142.145 kb on - strand, within DDA3937_RS18140 at 4142.145 kb on - strand, within DDA3937_RS18140 at 4142.162 kb on + strand, within DDA3937_RS18140 at 4142.162 kb on + strand, within DDA3937_RS18140 at 4142.162 kb on + strand, within DDA3937_RS18140 at 4142.162 kb on + strand, within DDA3937_RS18140 at 4142.163 kb on - strand, within DDA3937_RS18140 at 4142.163 kb on - strand, within DDA3937_RS18140 at 4142.163 kb on - strand, within DDA3937_RS18140 at 4142.163 kb on - strand, within DDA3937_RS18140 at 4142.165 kb on + strand, within DDA3937_RS18140 at 4142.166 kb on - strand, within DDA3937_RS18140 at 4142.166 kb on - strand, within DDA3937_RS18140 at 4142.408 kb on + strand, within DDA3937_RS18140 at 4142.408 kb on + strand, within DDA3937_RS18140 at 4142.408 kb on + strand, within DDA3937_RS18140 at 4142.409 kb on - strand, within DDA3937_RS18140 at 4142.411 kb on + strand, within DDA3937_RS18140 at 4142.411 kb on + strand, within DDA3937_RS18140 at 4142.411 kb on + strand, within DDA3937_RS18140 at 4142.449 kb on + strand, within DDA3937_RS18140 at 4142.450 kb on - strand, within DDA3937_RS18140 at 4142.450 kb on - strand, within DDA3937_RS18140 at 4142.450 kb on - strand, within DDA3937_RS18140
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 4,139,686 + -4.0 4,139,686 + -2.1 4,139,687 - -2.5 4,139,761 + -1.9 4,139,796 - -2.9 4,139,796 - -3.6 4,139,796 - -0.7 4,139,796 - -2.8 4,139,830 + -3.8 4,139,830 + -0.9 4,139,830 + +1.4 4,139,830 + -1.7 4,139,830 + -3.2 4,139,831 - -2.5 4,140,009 - DDA3937_RS18130 0.33 -2.1 4,140,138 - DDA3937_RS18130 0.54 -2.0 4,140,284 + DDA3937_RS18130 0.79 -3.7 4,140,285 - DDA3937_RS18130 0.79 -2.9 4,140,298 - DDA3937_RS18130 0.81 +0.3 4,140,380 + -0.9 4,140,444 + -1.1 4,140,494 + -1.9 4,140,494 + +0.6 4,140,494 + -0.1 4,140,495 - -1.5 4,140,495 - -0.1 4,140,495 - -0.4 4,140,495 - +0.3 4,140,536 - +0.7 4,140,845 + DDA3937_RS18135 0.29 +0.9 4,140,931 + -0.1 4,140,996 + DDA3937_RS18135 0.46 -2.8 4,140,996 + DDA3937_RS18135 0.46 -0.9 4,140,997 - DDA3937_RS18135 0.46 -1.5 4,141,227 + DDA3937_RS18135 0.72 -3.5 4,141,227 + DDA3937_RS18135 0.72 +1.7 4,141,229 + DDA3937_RS18135 0.72 -1.6 4,141,229 + DDA3937_RS18135 0.72 -2.1 4,141,278 + -1.1 4,141,278 + DDA3937_RS18135 0.78 -0.7 4,141,280 + DDA3937_RS18135 0.78 -0.7 4,141,280 + DDA3937_RS18135 0.78 -1.4 4,141,281 - DDA3937_RS18135 0.78 -1.5 4,141,397 - +0.1 4,141,444 - -1.9 4,141,458 + +0.3 4,141,458 + -0.3 4,141,459 - -1.7 4,141,491 - -0.1 4,141,491 - -1.1 4,141,491 - -0.1 4,141,533 + -1.7 4,141,534 - -0.2 4,141,534 - -0.2 4,141,534 - -0.2 4,141,534 - -1.1 4,141,616 - -2.4 4,141,689 + +0.3 4,141,694 + -0.3 4,141,699 - +0.1 4,141,705 - -0.7 4,141,715 + +0.9 4,141,865 + -0.4 4,142,030 + -0.4 4,142,030 + -1.9 4,142,030 + +0.1 4,142,030 + -1.5 4,142,030 + -2.0 4,142,031 - -1.4 4,142,031 - -0.8 4,142,063 + -0.9 4,142,063 + -1.1 4,142,063 + -0.3 4,142,064 - -0.7 4,142,064 - +0.3 4,142,064 - -1.9 4,142,064 - -1.4 4,142,145 - DDA3937_RS18140 0.15 -0.7 4,142,145 - DDA3937_RS18140 0.15 -0.4 4,142,145 - DDA3937_RS18140 0.15 +1.3 4,142,162 + DDA3937_RS18140 0.16 -0.8 4,142,162 + DDA3937_RS18140 0.16 -1.1 4,142,162 + DDA3937_RS18140 0.16 +0.9 4,142,162 + DDA3937_RS18140 0.16 +0.3 4,142,163 - DDA3937_RS18140 0.16 -0.5 4,142,163 - DDA3937_RS18140 0.16 -0.7 4,142,163 - DDA3937_RS18140 0.16 -0.9 4,142,163 - DDA3937_RS18140 0.16 +0.8 4,142,165 + DDA3937_RS18140 0.16 -3.7 4,142,166 - DDA3937_RS18140 0.17 +0.7 4,142,166 - DDA3937_RS18140 0.17 -0.3 4,142,408 + DDA3937_RS18140 0.34 +2.0 4,142,408 + DDA3937_RS18140 0.34 -0.4 4,142,408 + DDA3937_RS18140 0.34 -0.2 4,142,409 - DDA3937_RS18140 0.34 -0.7 4,142,411 + DDA3937_RS18140 0.34 -2.2 4,142,411 + DDA3937_RS18140 0.34 +1.8 4,142,411 + DDA3937_RS18140 0.34 +0.5 4,142,449 + DDA3937_RS18140 0.37 +0.8 4,142,450 - DDA3937_RS18140 0.37 +2.5 4,142,450 - DDA3937_RS18140 0.37 -0.5 4,142,450 - DDA3937_RS18140 0.37 +1.4
Or see this region's nucleotide sequence