Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS05595 and DDA3937_RS05600 are separated by 16 nucleotides DDA3937_RS05600 and DDA3937_RS05605 overlap by 1 nucleotides DDA3937_RS05605 and DDA3937_RS05610 overlap by 11 nucleotides
DDA3937_RS05595: DDA3937_RS05595 - protoheme IX farnesyltransferase, at 1,249,533 to 1,250,423
_RS05595
DDA3937_RS05600: DDA3937_RS05600 - cytochrome o ubiquinol oxidase subunit IV, at 1,250,440 to 1,250,772
_RS05600
DDA3937_RS05605: DDA3937_RS05605 - cytochrome o ubiquinol oxidase subunit III, at 1,250,772 to 1,251,386
_RS05605
DDA3937_RS05610: DDA3937_RS05610 - cytochrome o ubiquinol oxidase subunit I, at 1,251,376 to 1,253,367
_RS05610
Position (kb)
1250
1251
1252 Strain fitness (log2 ratio)
-1
0
1
2 at 1249.790 kb on + strand, within DDA3937_RS05595 at 1249.800 kb on - strand, within DDA3937_RS05595 at 1250.320 kb on + strand, within DDA3937_RS05595 at 1250.418 kb on - strand at 1250.418 kb on - strand at 1251.108 kb on - strand, within DDA3937_RS05605 at 1251.458 kb on + strand at 1251.458 kb on + strand at 1251.462 kb on - strand at 1251.462 kb on - strand at 1251.954 kb on - strand, within DDA3937_RS05610 at 1251.964 kb on - strand, within DDA3937_RS05610 at 1252.142 kb on - strand, within DDA3937_RS05610
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,249,790 + DDA3937_RS05595 0.29 -0.8 1,249,800 - DDA3937_RS05595 0.30 -0.6 1,250,320 + DDA3937_RS05595 0.88 -1.1 1,250,418 - -1.2 1,250,418 - -1.3 1,251,108 - DDA3937_RS05605 0.55 +0.0 1,251,458 + +2.6 1,251,458 + -1.3 1,251,462 - +0.9 1,251,462 - -0.9 1,251,954 - DDA3937_RS05610 0.29 +0.4 1,251,964 - DDA3937_RS05610 0.30 +0.2 1,252,142 - DDA3937_RS05610 0.38 -1.3
Or see this region's nucleotide sequence