Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS01165 and DDA3937_RS01170 are separated by 1 nucleotides DDA3937_RS01170 and DDA3937_RS01175 overlap by 1 nucleotides DDA3937_RS01175 and DDA3937_RS01180 are separated by 431 nucleotides DDA3937_RS01180 and DDA3937_RS01185 are separated by 103 nucleotides
DDA3937_RS01165: DDA3937_RS01165 - HesA/MoeB/ThiF family protein, at 266,251 to 267,000
_RS01165
DDA3937_RS01170: DDA3937_RS01170 - thiamine phosphate synthase, at 267,002 to 267,637
_RS01170
DDA3937_RS01175: DDA3937_RS01175 - phosphomethylpyrimidine synthase ThiC, at 267,637 to 269,508
_RS01175
DDA3937_RS01180: DDA3937_RS01180 - Rsd/AlgQ family anti-sigma factor, at 269,940 to 270,389
_RS01180
DDA3937_RS01185: DDA3937_RS01185 - NAD(+) diphosphatase, at 270,493 to 271,266
_RS01185
Position (kb) 267
268
269
270 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 266.773 kb on - strand, within DDA3937_RS01165 at 266.861 kb on + strand, within DDA3937_RS01165 at 266.861 kb on + strand, within DDA3937_RS01165 at 266.862 kb on - strand, within DDA3937_RS01165 at 266.862 kb on - strand, within DDA3937_RS01165 at 266.920 kb on + strand, within DDA3937_RS01165 at 266.920 kb on + strand, within DDA3937_RS01165 at 266.921 kb on - strand, within DDA3937_RS01165 at 266.973 kb on - strand at 266.979 kb on + strand at 266.980 kb on - strand at 267.250 kb on + strand, within DDA3937_RS01170 at 267.250 kb on + strand, within DDA3937_RS01170 at 267.250 kb on + strand, within DDA3937_RS01170 at 267.250 kb on + strand, within DDA3937_RS01170 at 267.267 kb on + strand, within DDA3937_RS01170 at 267.267 kb on + strand, within DDA3937_RS01170 at 267.267 kb on + strand, within DDA3937_RS01170 at 267.267 kb on + strand, within DDA3937_RS01170 at 267.268 kb on - strand, within DDA3937_RS01170 at 267.268 kb on - strand, within DDA3937_RS01170 at 267.386 kb on + strand, within DDA3937_RS01170 at 267.386 kb on + strand, within DDA3937_RS01170 at 267.387 kb on - strand, within DDA3937_RS01170 at 267.387 kb on - strand, within DDA3937_RS01170 at 267.387 kb on - strand, within DDA3937_RS01170 at 267.393 kb on - strand, within DDA3937_RS01170 at 267.400 kb on - strand, within DDA3937_RS01170 at 267.400 kb on - strand, within DDA3937_RS01170 at 267.430 kb on - strand, within DDA3937_RS01170 at 267.674 kb on + strand at 267.765 kb on - strand at 268.013 kb on + strand, within DDA3937_RS01175 at 268.014 kb on - strand, within DDA3937_RS01175 at 268.044 kb on + strand, within DDA3937_RS01175 at 268.044 kb on + strand, within DDA3937_RS01175 at 268.044 kb on + strand, within DDA3937_RS01175 at 268.045 kb on - strand, within DDA3937_RS01175 at 268.045 kb on - strand, within DDA3937_RS01175 at 268.079 kb on + strand, within DDA3937_RS01175 at 268.079 kb on + strand, within DDA3937_RS01175 at 268.079 kb on + strand, within DDA3937_RS01175 at 268.080 kb on - strand, within DDA3937_RS01175 at 268.080 kb on - strand, within DDA3937_RS01175 at 268.177 kb on + strand, within DDA3937_RS01175 at 268.279 kb on + strand, within DDA3937_RS01175 at 268.279 kb on + strand, within DDA3937_RS01175 at 268.279 kb on + strand, within DDA3937_RS01175 at 268.279 kb on + strand, within DDA3937_RS01175 at 268.279 kb on + strand, within DDA3937_RS01175 at 268.279 kb on + strand, within DDA3937_RS01175 at 268.279 kb on + strand, within DDA3937_RS01175 at 268.280 kb on - strand, within DDA3937_RS01175 at 268.280 kb on - strand, within DDA3937_RS01175 at 268.280 kb on - strand, within DDA3937_RS01175 at 268.280 kb on - strand, within DDA3937_RS01175 at 268.280 kb on - strand, within DDA3937_RS01175 at 268.280 kb on - strand, within DDA3937_RS01175 at 268.280 kb on - strand, within DDA3937_RS01175 at 268.286 kb on + strand, within DDA3937_RS01175 at 268.286 kb on + strand, within DDA3937_RS01175 at 268.414 kb on - strand, within DDA3937_RS01175 at 268.577 kb on + strand, within DDA3937_RS01175 at 268.577 kb on + strand, within DDA3937_RS01175 at 268.577 kb on + strand, within DDA3937_RS01175 at 268.673 kb on + strand, within DDA3937_RS01175 at 268.673 kb on + strand, within DDA3937_RS01175 at 268.673 kb on + strand, within DDA3937_RS01175 at 268.674 kb on - strand, within DDA3937_RS01175 at 268.674 kb on - strand, within DDA3937_RS01175 at 268.674 kb on - strand, within DDA3937_RS01175 at 268.738 kb on - strand, within DDA3937_RS01175 at 268.738 kb on - strand, within DDA3937_RS01175 at 268.739 kb on + strand, within DDA3937_RS01175 at 268.739 kb on + strand, within DDA3937_RS01175 at 268.739 kb on + strand, within DDA3937_RS01175 at 268.739 kb on + strand, within DDA3937_RS01175 at 268.740 kb on - strand, within DDA3937_RS01175 at 268.740 kb on - strand, within DDA3937_RS01175 at 268.839 kb on + strand, within DDA3937_RS01175 at 268.840 kb on - strand, within DDA3937_RS01175 at 269.109 kb on - strand, within DDA3937_RS01175 at 269.211 kb on + strand, within DDA3937_RS01175 at 269.234 kb on - strand, within DDA3937_RS01175 at 269.296 kb on + strand, within DDA3937_RS01175 at 269.297 kb on - strand, within DDA3937_RS01175 at 269.342 kb on + strand at 269.342 kb on + strand at 269.409 kb on + strand at 269.410 kb on - strand at 269.462 kb on + strand at 269.463 kb on - strand at 269.616 kb on + strand at 269.616 kb on + strand at 269.617 kb on - strand at 269.617 kb on - strand at 269.623 kb on - strand at 269.641 kb on - strand at 269.676 kb on + strand at 269.769 kb on + strand at 269.769 kb on + strand at 269.769 kb on + strand at 269.771 kb on + strand at 269.771 kb on + strand at 269.771 kb on + strand at 269.771 kb on + strand at 269.771 kb on + strand at 269.771 kb on + strand at 269.771 kb on + strand at 269.772 kb on - strand at 269.772 kb on - strand at 269.772 kb on - strand at 269.772 kb on - strand at 269.772 kb on - strand at 269.775 kb on + strand at 269.775 kb on + strand at 269.775 kb on + strand at 269.776 kb on - strand at 269.776 kb on - strand at 269.776 kb on - strand at 269.852 kb on + strand at 269.902 kb on + strand at 269.903 kb on - strand at 269.918 kb on + strand at 269.918 kb on + strand at 269.919 kb on - strand at 269.919 kb on - strand at 270.076 kb on + strand, within DDA3937_RS01180 at 270.076 kb on + strand, within DDA3937_RS01180 at 270.076 kb on + strand, within DDA3937_RS01180 at 270.077 kb on - strand, within DDA3937_RS01180 at 270.077 kb on - strand, within DDA3937_RS01180 at 270.080 kb on + strand, within DDA3937_RS01180 at 270.184 kb on + strand, within DDA3937_RS01180 at 270.184 kb on + strand, within DDA3937_RS01180 at 270.184 kb on + strand, within DDA3937_RS01180 at 270.184 kb on + strand, within DDA3937_RS01180 at 270.185 kb on - strand, within DDA3937_RS01180 at 270.211 kb on + strand, within DDA3937_RS01180 at 270.211 kb on + strand at 270.211 kb on + strand, within DDA3937_RS01180 at 270.211 kb on + strand, within DDA3937_RS01180 at 270.211 kb on + strand, within DDA3937_RS01180 at 270.211 kb on + strand, within DDA3937_RS01180 at 270.212 kb on - strand, within DDA3937_RS01180 at 270.259 kb on - strand, within DDA3937_RS01180 at 270.289 kb on + strand, within DDA3937_RS01180 at 270.289 kb on + strand, within DDA3937_RS01180 at 270.289 kb on + strand, within DDA3937_RS01180 at 270.289 kb on + strand, within DDA3937_RS01180 at 270.290 kb on - strand, within DDA3937_RS01180 at 270.290 kb on - strand, within DDA3937_RS01180 at 270.292 kb on + strand, within DDA3937_RS01180 at 270.292 kb on + strand, within DDA3937_RS01180 at 270.292 kb on + strand, within DDA3937_RS01180 at 270.292 kb on + strand, within DDA3937_RS01180 at 270.292 kb on + strand, within DDA3937_RS01180 at 270.293 kb on - strand, within DDA3937_RS01180 at 270.293 kb on - strand, within DDA3937_RS01180 at 270.293 kb on - strand, within DDA3937_RS01180 at 270.293 kb on - strand, within DDA3937_RS01180 at 270.293 kb on - strand, within DDA3937_RS01180 at 270.293 kb on - strand, within DDA3937_RS01180 at 270.293 kb on - strand, within DDA3937_RS01180 at 270.343 kb on + strand, within DDA3937_RS01180 at 270.343 kb on + strand, within DDA3937_RS01180 at 270.344 kb on - strand, within DDA3937_RS01180 at 270.344 kb on - strand, within DDA3937_RS01180 at 270.344 kb on - strand, within DDA3937_RS01180 at 270.344 kb on - strand, within DDA3937_RS01180 at 270.375 kb on + strand at 270.375 kb on + strand at 270.376 kb on - strand at 270.409 kb on + strand at 270.409 kb on + strand at 270.409 kb on + strand at 270.409 kb on + strand at 270.430 kb on + strand at 270.430 kb on + strand at 270.431 kb on - strand at 270.431 kb on - strand at 270.436 kb on + strand at 270.437 kb on - strand at 270.442 kb on + strand at 270.448 kb on + strand at 270.448 kb on + strand at 270.454 kb on + strand at 270.454 kb on + strand at 270.454 kb on + strand at 270.454 kb on + strand at 270.455 kb on - strand at 270.474 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 266,773 - DDA3937_RS01165 0.70 -1.7 266,861 + DDA3937_RS01165 0.81 +0.3 266,861 + DDA3937_RS01165 0.81 -1.7 266,862 - DDA3937_RS01165 0.81 -2.5 266,862 - DDA3937_RS01165 0.81 -2.2 266,920 + DDA3937_RS01165 0.89 -0.7 266,920 + DDA3937_RS01165 0.89 -0.5 266,921 - DDA3937_RS01165 0.89 -1.2 266,973 - -1.0 266,979 + -0.9 266,980 - -0.9 267,250 + DDA3937_RS01170 0.39 -1.8 267,250 + DDA3937_RS01170 0.39 -2.3 267,250 + DDA3937_RS01170 0.39 -1.4 267,250 + DDA3937_RS01170 0.39 -0.7 267,267 + DDA3937_RS01170 0.42 -2.6 267,267 + DDA3937_RS01170 0.42 -4.2 267,267 + DDA3937_RS01170 0.42 -2.8 267,267 + DDA3937_RS01170 0.42 -2.6 267,268 - DDA3937_RS01170 0.42 -1.5 267,268 - DDA3937_RS01170 0.42 -2.0 267,386 + DDA3937_RS01170 0.60 -2.5 267,386 + DDA3937_RS01170 0.60 -0.9 267,387 - DDA3937_RS01170 0.61 -0.9 267,387 - DDA3937_RS01170 0.61 +0.4 267,387 - DDA3937_RS01170 0.61 -1.2 267,393 - DDA3937_RS01170 0.61 -1.1 267,400 - DDA3937_RS01170 0.63 -1.9 267,400 - DDA3937_RS01170 0.63 -0.9 267,430 - DDA3937_RS01170 0.67 -3.1 267,674 + -1.1 267,765 - -3.4 268,013 + DDA3937_RS01175 0.20 -3.4 268,014 - DDA3937_RS01175 0.20 +1.2 268,044 + DDA3937_RS01175 0.22 -3.0 268,044 + DDA3937_RS01175 0.22 -2.1 268,044 + DDA3937_RS01175 0.22 -2.8 268,045 - DDA3937_RS01175 0.22 -1.2 268,045 - DDA3937_RS01175 0.22 -2.9 268,079 + DDA3937_RS01175 0.24 -1.0 268,079 + DDA3937_RS01175 0.24 -0.8 268,079 + DDA3937_RS01175 0.24 -2.0 268,080 - DDA3937_RS01175 0.24 -1.9 268,080 - DDA3937_RS01175 0.24 +0.7 268,177 + DDA3937_RS01175 0.29 -3.6 268,279 + DDA3937_RS01175 0.34 -2.5 268,279 + DDA3937_RS01175 0.34 -1.3 268,279 + DDA3937_RS01175 0.34 -1.5 268,279 + DDA3937_RS01175 0.34 -1.4 268,279 + DDA3937_RS01175 0.34 -3.1 268,279 + DDA3937_RS01175 0.34 -3.8 268,279 + DDA3937_RS01175 0.34 -1.1 268,280 - DDA3937_RS01175 0.34 -0.5 268,280 - DDA3937_RS01175 0.34 -2.5 268,280 - DDA3937_RS01175 0.34 -0.6 268,280 - DDA3937_RS01175 0.34 +1.1 268,280 - DDA3937_RS01175 0.34 -2.1 268,280 - DDA3937_RS01175 0.34 +0.1 268,280 - DDA3937_RS01175 0.34 -0.9 268,286 + DDA3937_RS01175 0.35 -1.7 268,286 + DDA3937_RS01175 0.35 -2.8 268,414 - DDA3937_RS01175 0.42 -0.5 268,577 + DDA3937_RS01175 0.50 -0.9 268,577 + DDA3937_RS01175 0.50 -3.0 268,577 + DDA3937_RS01175 0.50 -0.5 268,673 + DDA3937_RS01175 0.55 -3.5 268,673 + DDA3937_RS01175 0.55 -0.3 268,673 + DDA3937_RS01175 0.55 -2.6 268,674 - DDA3937_RS01175 0.55 -4.7 268,674 - DDA3937_RS01175 0.55 -1.6 268,674 - DDA3937_RS01175 0.55 -1.5 268,738 - DDA3937_RS01175 0.59 -2.1 268,738 - DDA3937_RS01175 0.59 -0.8 268,739 + DDA3937_RS01175 0.59 -0.9 268,739 + DDA3937_RS01175 0.59 -1.4 268,739 + DDA3937_RS01175 0.59 -3.3 268,739 + DDA3937_RS01175 0.59 -3.4 268,740 - DDA3937_RS01175 0.59 -2.3 268,740 - DDA3937_RS01175 0.59 -2.9 268,839 + DDA3937_RS01175 0.64 -2.8 268,840 - DDA3937_RS01175 0.64 -0.9 269,109 - DDA3937_RS01175 0.79 -2.3 269,211 + DDA3937_RS01175 0.84 -2.8 269,234 - DDA3937_RS01175 0.85 -2.0 269,296 + DDA3937_RS01175 0.89 -3.1 269,297 - DDA3937_RS01175 0.89 -1.9 269,342 + -1.8 269,342 + -2.8 269,409 + -1.7 269,410 - +0.1 269,462 + -1.5 269,463 - -1.8 269,616 + -1.2 269,616 + -2.2 269,617 - -1.7 269,617 - -0.2 269,623 - -1.5 269,641 - +1.1 269,676 + -3.0 269,769 + +1.1 269,769 + -3.0 269,769 + -1.9 269,771 + -1.9 269,771 + -3.1 269,771 + -2.8 269,771 + -0.7 269,771 + -1.0 269,771 + -2.8 269,771 + -2.2 269,772 - +1.7 269,772 - -1.8 269,772 - -1.5 269,772 - -1.2 269,772 - -0.6 269,775 + -0.6 269,775 + -2.0 269,775 + -1.6 269,776 - -1.2 269,776 - -2.5 269,776 - -3.6 269,852 + -1.5 269,902 + -2.6 269,903 - +0.9 269,918 + -2.1 269,918 + -0.7 269,919 - +0.1 269,919 - -0.3 270,076 + DDA3937_RS01180 0.30 +0.8 270,076 + DDA3937_RS01180 0.30 +0.5 270,076 + DDA3937_RS01180 0.30 +1.4 270,077 - DDA3937_RS01180 0.30 -1.7 270,077 - DDA3937_RS01180 0.30 -0.5 270,080 + DDA3937_RS01180 0.31 -2.8 270,184 + DDA3937_RS01180 0.54 -0.8 270,184 + DDA3937_RS01180 0.54 +1.6 270,184 + DDA3937_RS01180 0.54 -0.5 270,184 + DDA3937_RS01180 0.54 +0.0 270,185 - DDA3937_RS01180 0.54 -1.3 270,211 + DDA3937_RS01180 0.60 -1.4 270,211 + +2.1 270,211 + DDA3937_RS01180 0.60 +2.0 270,211 + DDA3937_RS01180 0.60 -1.4 270,211 + DDA3937_RS01180 0.60 -0.9 270,211 + DDA3937_RS01180 0.60 -0.2 270,212 - DDA3937_RS01180 0.60 +0.9 270,259 - DDA3937_RS01180 0.71 -1.2 270,289 + DDA3937_RS01180 0.78 +1.1 270,289 + DDA3937_RS01180 0.78 +0.1 270,289 + DDA3937_RS01180 0.78 +0.4 270,289 + DDA3937_RS01180 0.78 -0.1 270,290 - DDA3937_RS01180 0.78 -0.5 270,290 - DDA3937_RS01180 0.78 -0.1 270,292 + DDA3937_RS01180 0.78 +1.1 270,292 + DDA3937_RS01180 0.78 -1.7 270,292 + DDA3937_RS01180 0.78 -1.2 270,292 + DDA3937_RS01180 0.78 -1.3 270,292 + DDA3937_RS01180 0.78 +0.4 270,293 - DDA3937_RS01180 0.78 +0.3 270,293 - DDA3937_RS01180 0.78 -0.8 270,293 - DDA3937_RS01180 0.78 +0.6 270,293 - DDA3937_RS01180 0.78 +1.1 270,293 - DDA3937_RS01180 0.78 -0.2 270,293 - DDA3937_RS01180 0.78 -0.5 270,293 - DDA3937_RS01180 0.78 -0.6 270,343 + DDA3937_RS01180 0.90 +1.0 270,343 + DDA3937_RS01180 0.90 +2.6 270,344 - DDA3937_RS01180 0.90 -1.6 270,344 - DDA3937_RS01180 0.90 -0.9 270,344 - DDA3937_RS01180 0.90 +0.5 270,344 - DDA3937_RS01180 0.90 -0.8 270,375 + -1.7 270,375 + -1.2 270,376 - -0.4 270,409 + -0.0 270,409 + -0.2 270,409 + +1.0 270,409 + +3.1 270,430 + -0.5 270,430 + -0.1 270,431 - +0.3 270,431 - -0.2 270,436 + -0.6 270,437 - -0.9 270,442 + -1.2 270,448 + -0.2 270,448 + -0.9 270,454 + -2.7 270,454 + -0.9 270,454 + +1.5 270,454 + +0.1 270,455 - -0.6 270,474 - -1.2
Or see this region's nucleotide sequence