Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS18160 and DDA3937_RS18165 overlap by 17 nucleotides DDA3937_RS18165 and DDA3937_RS23340 are separated by 142 nucleotides DDA3937_RS23340 and DDA3937_RS18170 are separated by 113 nucleotides
DDA3937_RS18160: DDA3937_RS18160 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 4,146,606 to 4,148,216
_RS18160
DDA3937_RS18165: DDA3937_RS18165 - thiamine ABC transporter ATP-binding protein ThiQ, at 4,148,200 to 4,148,892
_RS18165
DDA3937_RS23340: DDA3937_RS23340 - ilvB operon leader peptide IvbL, at 4,149,035 to 4,149,148
_RS23340
DDA3937_RS18170: DDA3937_RS18170 - acetolactate synthase large subunit, at 4,149,262 to 4,150,926
_RS18170
Position (kb)
4149
4150 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4148.085 kb on + strand at 4148.085 kb on + strand at 4148.092 kb on - strand at 4148.162 kb on + strand at 4148.163 kb on - strand at 4148.163 kb on - strand at 4148.163 kb on - strand at 4148.163 kb on - strand at 4148.163 kb on - strand at 4148.225 kb on - strand at 4148.225 kb on - strand at 4148.230 kb on + strand at 4148.230 kb on + strand at 4148.231 kb on - strand at 4148.258 kb on + strand at 4148.825 kb on - strand at 4148.825 kb on - strand at 4148.825 kb on - strand at 4148.825 kb on - strand at 4148.926 kb on - strand at 4149.004 kb on + strand at 4149.004 kb on + strand at 4149.004 kb on + strand at 4149.004 kb on + strand at 4149.005 kb on - strand at 4149.022 kb on - strand at 4149.034 kb on + strand at 4149.034 kb on + strand at 4149.034 kb on + strand at 4149.035 kb on - strand at 4149.035 kb on - strand at 4149.035 kb on - strand at 4149.036 kb on + strand at 4149.036 kb on + strand at 4149.036 kb on + strand at 4149.036 kb on + strand at 4149.037 kb on - strand at 4149.037 kb on - strand at 4149.037 kb on - strand at 4149.037 kb on - strand at 4149.037 kb on - strand at 4149.065 kb on - strand, within DDA3937_RS23340 at 4149.076 kb on - strand, within DDA3937_RS23340 at 4149.078 kb on + strand, within DDA3937_RS23340 at 4149.078 kb on + strand, within DDA3937_RS23340 at 4149.079 kb on - strand, within DDA3937_RS23340 at 4149.168 kb on - strand at 4149.168 kb on - strand at 4149.168 kb on - strand at 4149.168 kb on - strand at 4149.168 kb on - strand at 4149.168 kb on - strand at 4149.212 kb on - strand at 4149.248 kb on + strand at 4149.255 kb on + strand at 4149.255 kb on + strand at 4149.261 kb on + strand at 4149.261 kb on + strand at 4149.261 kb on + strand at 4149.261 kb on + strand at 4149.261 kb on + strand at 4149.261 kb on + strand at 4149.262 kb on - strand at 4149.262 kb on - strand at 4149.262 kb on - strand at 4149.262 kb on - strand at 4149.262 kb on - strand at 4149.269 kb on - strand at 4149.270 kb on + strand at 4149.270 kb on + strand at 4149.270 kb on + strand at 4149.270 kb on + strand at 4149.270 kb on + strand at 4149.270 kb on + strand at 4149.271 kb on - strand at 4149.271 kb on - strand at 4149.358 kb on + strand at 4149.395 kb on + strand at 4149.396 kb on - strand at 4149.396 kb on - strand at 4149.613 kb on + strand, within DDA3937_RS18170 at 4149.613 kb on + strand, within DDA3937_RS18170 at 4149.614 kb on - strand, within DDA3937_RS18170 at 4149.614 kb on - strand, within DDA3937_RS18170 at 4149.646 kb on + strand, within DDA3937_RS18170 at 4149.646 kb on + strand, within DDA3937_RS18170 at 4149.646 kb on + strand, within DDA3937_RS18170 at 4149.646 kb on + strand, within DDA3937_RS18170 at 4149.646 kb on + strand, within DDA3937_RS18170 at 4149.647 kb on - strand, within DDA3937_RS18170 at 4149.647 kb on - strand, within DDA3937_RS18170 at 4149.647 kb on - strand, within DDA3937_RS18170 at 4149.647 kb on - strand, within DDA3937_RS18170 at 4149.752 kb on + strand, within DDA3937_RS18170 at 4149.753 kb on - strand, within DDA3937_RS18170 at 4149.753 kb on - strand, within DDA3937_RS18170 at 4149.801 kb on + strand, within DDA3937_RS18170 at 4149.802 kb on - strand, within DDA3937_RS18170 at 4149.897 kb on + strand, within DDA3937_RS18170 at 4150.014 kb on + strand, within DDA3937_RS18170 at 4150.041 kb on + strand, within DDA3937_RS18170 at 4150.041 kb on + strand, within DDA3937_RS18170 at 4150.041 kb on + strand, within DDA3937_RS18170 at 4150.041 kb on + strand, within DDA3937_RS18170 at 4150.041 kb on + strand, within DDA3937_RS18170 at 4150.041 kb on + strand, within DDA3937_RS18170 at 4150.041 kb on + strand, within DDA3937_RS18170 at 4150.041 kb on + strand, within DDA3937_RS18170 at 4150.041 kb on + strand, within DDA3937_RS18170 at 4150.042 kb on - strand, within DDA3937_RS18170 at 4150.042 kb on - strand, within DDA3937_RS18170 at 4150.042 kb on - strand, within DDA3937_RS18170 at 4150.047 kb on + strand, within DDA3937_RS18170 at 4150.047 kb on + strand, within DDA3937_RS18170 at 4150.048 kb on - strand, within DDA3937_RS18170 at 4150.048 kb on - strand, within DDA3937_RS18170 at 4150.048 kb on - strand, within DDA3937_RS18170 at 4150.048 kb on - strand, within DDA3937_RS18170 at 4150.048 kb on - strand, within DDA3937_RS18170
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 4,148,085 + +0.3 4,148,085 + -0.6 4,148,092 - +0.2 4,148,162 + -1.6 4,148,163 - -1.7 4,148,163 - +0.2 4,148,163 - -0.5 4,148,163 - -0.6 4,148,163 - +2.2 4,148,225 - +0.2 4,148,225 - +0.1 4,148,230 + -0.5 4,148,230 + -0.6 4,148,231 - -0.4 4,148,258 + +1.5 4,148,825 - +0.7 4,148,825 - +1.1 4,148,825 - -0.7 4,148,825 - -1.1 4,148,926 - +0.5 4,149,004 + -1.5 4,149,004 + -0.9 4,149,004 + -0.4 4,149,004 + -1.7 4,149,005 - -1.4 4,149,022 - -2.4 4,149,034 + +0.2 4,149,034 + +0.6 4,149,034 + +0.3 4,149,035 - -3.7 4,149,035 - -2.9 4,149,035 - -0.3 4,149,036 + -0.8 4,149,036 + -1.3 4,149,036 + -2.4 4,149,036 + -1.4 4,149,037 - -0.4 4,149,037 - -0.2 4,149,037 - -2.7 4,149,037 - -2.8 4,149,037 - -1.5 4,149,065 - DDA3937_RS23340 0.26 -0.7 4,149,076 - DDA3937_RS23340 0.36 -2.7 4,149,078 + DDA3937_RS23340 0.38 -0.9 4,149,078 + DDA3937_RS23340 0.38 +0.7 4,149,079 - DDA3937_RS23340 0.39 -1.3 4,149,168 - -2.6 4,149,168 - -0.3 4,149,168 - -1.9 4,149,168 - -2.1 4,149,168 - -2.3 4,149,168 - -1.3 4,149,212 - -0.3 4,149,248 + -0.2 4,149,255 + -1.7 4,149,255 + -2.4 4,149,261 + -2.8 4,149,261 + -1.6 4,149,261 + -2.4 4,149,261 + -2.4 4,149,261 + -2.3 4,149,261 + -2.3 4,149,262 - -3.9 4,149,262 - -2.8 4,149,262 - -0.8 4,149,262 - -1.9 4,149,262 - -1.7 4,149,269 - -2.7 4,149,270 + -1.9 4,149,270 + -1.5 4,149,270 + -0.9 4,149,270 + -1.2 4,149,270 + -1.0 4,149,270 + -1.4 4,149,271 - +2.1 4,149,271 - -0.3 4,149,358 + -2.6 4,149,395 + -1.0 4,149,396 - -0.7 4,149,396 - -3.5 4,149,613 + DDA3937_RS18170 0.21 -1.8 4,149,613 + DDA3937_RS18170 0.21 -2.9 4,149,614 - DDA3937_RS18170 0.21 -1.5 4,149,614 - DDA3937_RS18170 0.21 +0.3 4,149,646 + DDA3937_RS18170 0.23 +1.1 4,149,646 + DDA3937_RS18170 0.23 -0.5 4,149,646 + DDA3937_RS18170 0.23 -1.5 4,149,646 + DDA3937_RS18170 0.23 +0.5 4,149,646 + DDA3937_RS18170 0.23 -1.5 4,149,647 - DDA3937_RS18170 0.23 -3.5 4,149,647 - DDA3937_RS18170 0.23 -0.9 4,149,647 - DDA3937_RS18170 0.23 -4.0 4,149,647 - DDA3937_RS18170 0.23 -0.4 4,149,752 + DDA3937_RS18170 0.29 -0.6 4,149,753 - DDA3937_RS18170 0.29 -1.7 4,149,753 - DDA3937_RS18170 0.29 -0.9 4,149,801 + DDA3937_RS18170 0.32 -2.3 4,149,802 - DDA3937_RS18170 0.32 -1.7 4,149,897 + DDA3937_RS18170 0.38 -3.0 4,150,014 + DDA3937_RS18170 0.45 +2.2 4,150,041 + DDA3937_RS18170 0.47 -2.1 4,150,041 + DDA3937_RS18170 0.47 -1.2 4,150,041 + DDA3937_RS18170 0.47 -2.4 4,150,041 + DDA3937_RS18170 0.47 -2.2 4,150,041 + DDA3937_RS18170 0.47 -1.8 4,150,041 + DDA3937_RS18170 0.47 -2.5 4,150,041 + DDA3937_RS18170 0.47 +0.1 4,150,041 + DDA3937_RS18170 0.47 -1.5 4,150,041 + DDA3937_RS18170 0.47 -1.9 4,150,042 - DDA3937_RS18170 0.47 +0.4 4,150,042 - DDA3937_RS18170 0.47 -1.5 4,150,042 - DDA3937_RS18170 0.47 -0.0 4,150,047 + DDA3937_RS18170 0.47 -2.1 4,150,047 + DDA3937_RS18170 0.47 -2.7 4,150,048 - DDA3937_RS18170 0.47 -1.5 4,150,048 - DDA3937_RS18170 0.47 -1.3 4,150,048 - DDA3937_RS18170 0.47 -1.5 4,150,048 - DDA3937_RS18170 0.47 -1.9 4,150,048 - DDA3937_RS18170 0.47 -0.9
Or see this region's nucleotide sequence