Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS16850 and DDA3937_RS16855 are separated by 62 nucleotides DDA3937_RS16855 and DDA3937_RS16860 are separated by 114 nucleotides DDA3937_RS16860 and DDA3937_RS16865 overlap by 4 nucleotides DDA3937_RS16865 and DDA3937_RS16870 are separated by 115 nucleotides
DDA3937_RS16850: DDA3937_RS16850 - methionine ABC transporter permease MetI, at 3,831,987 to 3,832,640
_RS16850
DDA3937_RS16855: DDA3937_RS16855 - MetQ/NlpA family lipoprotein, at 3,832,703 to 3,833,518
_RS16855
DDA3937_RS16860: DDA3937_RS16860 - Rcs stress response system protein RcsF, at 3,833,633 to 3,834,040
_RS16860
DDA3937_RS16865: DDA3937_RS16865 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 3,834,037 to 3,834,744
_RS16865
DDA3937_RS16870: DDA3937_RS16870 - proline--tRNA ligase, at 3,834,860 to 3,836,578
_RS16870
Position (kb)
3833
3834
3835 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3832.634 kb on + strand at 3832.634 kb on + strand at 3832.635 kb on - strand at 3832.638 kb on + strand at 3832.648 kb on + strand at 3832.648 kb on + strand at 3832.661 kb on - strand at 3832.681 kb on - strand at 3832.681 kb on - strand at 3832.689 kb on - strand at 3832.689 kb on - strand at 3832.689 kb on - strand at 3832.711 kb on + strand at 3832.712 kb on - strand at 3832.712 kb on - strand at 3832.712 kb on - strand at 3832.793 kb on - strand, within DDA3937_RS16855 at 3832.801 kb on + strand, within DDA3937_RS16855 at 3832.872 kb on - strand, within DDA3937_RS16855 at 3832.895 kb on - strand, within DDA3937_RS16855 at 3832.907 kb on + strand, within DDA3937_RS16855 at 3832.908 kb on - strand, within DDA3937_RS16855 at 3832.908 kb on - strand, within DDA3937_RS16855 at 3832.908 kb on - strand, within DDA3937_RS16855 at 3832.908 kb on - strand, within DDA3937_RS16855 at 3832.974 kb on - strand, within DDA3937_RS16855 at 3833.003 kb on + strand, within DDA3937_RS16855 at 3833.004 kb on - strand at 3833.005 kb on + strand, within DDA3937_RS16855 at 3833.005 kb on + strand, within DDA3937_RS16855 at 3833.006 kb on - strand, within DDA3937_RS16855 at 3833.006 kb on - strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.315 kb on + strand, within DDA3937_RS16855 at 3833.316 kb on - strand, within DDA3937_RS16855 at 3833.316 kb on - strand, within DDA3937_RS16855 at 3833.316 kb on - strand, within DDA3937_RS16855 at 3833.316 kb on - strand, within DDA3937_RS16855 at 3833.316 kb on - strand, within DDA3937_RS16855 at 3833.316 kb on - strand, within DDA3937_RS16855 at 3833.316 kb on - strand, within DDA3937_RS16855 at 3833.316 kb on - strand, within DDA3937_RS16855 at 3833.316 kb on - strand, within DDA3937_RS16855 at 3833.381 kb on + strand, within DDA3937_RS16855 at 3833.442 kb on + strand at 3833.443 kb on - strand at 3833.451 kb on - strand at 3833.466 kb on + strand at 3833.467 kb on - strand at 3833.467 kb on - strand at 3833.487 kb on + strand at 3833.487 kb on + strand at 3833.488 kb on - strand at 3833.491 kb on + strand at 3833.491 kb on + strand at 3833.491 kb on + strand at 3833.491 kb on + strand at 3833.491 kb on + strand at 3833.492 kb on - strand at 3833.516 kb on + strand at 3833.516 kb on + strand at 3833.517 kb on - strand at 3833.521 kb on + strand at 3833.522 kb on - strand at 3833.522 kb on - strand at 3833.522 kb on - strand at 3833.524 kb on + strand at 3833.524 kb on + strand at 3833.524 kb on + strand at 3833.524 kb on + strand at 3833.524 kb on + strand at 3833.525 kb on - strand at 3833.525 kb on - strand at 3833.528 kb on + strand at 3833.528 kb on + strand at 3833.529 kb on - strand at 3833.529 kb on - strand at 3833.533 kb on + strand at 3833.534 kb on - strand at 3833.534 kb on - strand at 3833.585 kb on + strand at 3833.585 kb on + strand at 3833.611 kb on + strand at 3833.633 kb on - strand at 3833.643 kb on + strand at 3833.644 kb on - strand at 3833.644 kb on - strand at 3833.791 kb on + strand, within DDA3937_RS16860 at 3833.792 kb on - strand, within DDA3937_RS16860 at 3833.792 kb on - strand, within DDA3937_RS16860 at 3833.812 kb on - strand, within DDA3937_RS16860 at 3833.876 kb on - strand, within DDA3937_RS16860 at 3833.974 kb on + strand, within DDA3937_RS16860 at 3833.975 kb on - strand, within DDA3937_RS16860 at 3833.975 kb on - strand, within DDA3937_RS16860 at 3833.975 kb on - strand, within DDA3937_RS16860 at 3833.990 kb on + strand, within DDA3937_RS16860 at 3834.082 kb on + strand at 3834.083 kb on - strand at 3834.083 kb on - strand at 3834.083 kb on - strand at 3834.083 kb on - strand at 3834.083 kb on - strand at 3834.083 kb on - strand at 3834.083 kb on - strand at 3834.084 kb on + strand at 3834.085 kb on - strand at 3834.085 kb on - strand at 3834.085 kb on - strand at 3834.143 kb on + strand, within DDA3937_RS16865 at 3834.143 kb on + strand, within DDA3937_RS16865 at 3834.143 kb on + strand, within DDA3937_RS16865 at 3834.143 kb on + strand, within DDA3937_RS16865 at 3834.143 kb on + strand, within DDA3937_RS16865 at 3834.143 kb on + strand, within DDA3937_RS16865 at 3834.143 kb on + strand, within DDA3937_RS16865 at 3834.143 kb on + strand, within DDA3937_RS16865 at 3834.144 kb on - strand, within DDA3937_RS16865 at 3834.144 kb on - strand, within DDA3937_RS16865 at 3834.144 kb on - strand, within DDA3937_RS16865 at 3834.144 kb on - strand, within DDA3937_RS16865 at 3834.160 kb on + strand, within DDA3937_RS16865 at 3834.160 kb on + strand, within DDA3937_RS16865 at 3834.160 kb on + strand, within DDA3937_RS16865 at 3834.161 kb on - strand, within DDA3937_RS16865 at 3834.161 kb on - strand, within DDA3937_RS16865 at 3834.161 kb on - strand, within DDA3937_RS16865 at 3834.162 kb on + strand, within DDA3937_RS16865 at 3834.162 kb on + strand, within DDA3937_RS16865 at 3834.162 kb on + strand, within DDA3937_RS16865 at 3834.162 kb on + strand, within DDA3937_RS16865 at 3834.162 kb on + strand, within DDA3937_RS16865 at 3834.163 kb on - strand, within DDA3937_RS16865 at 3834.163 kb on - strand, within DDA3937_RS16865 at 3834.163 kb on - strand, within DDA3937_RS16865 at 3834.163 kb on - strand, within DDA3937_RS16865 at 3834.339 kb on + strand, within DDA3937_RS16865 at 3834.339 kb on + strand, within DDA3937_RS16865 at 3834.339 kb on + strand, within DDA3937_RS16865 at 3834.339 kb on + strand, within DDA3937_RS16865 at 3834.339 kb on + strand at 3834.340 kb on - strand, within DDA3937_RS16865 at 3834.340 kb on - strand, within DDA3937_RS16865 at 3834.340 kb on - strand, within DDA3937_RS16865 at 3834.340 kb on - strand, within DDA3937_RS16865 at 3834.416 kb on + strand, within DDA3937_RS16865 at 3834.417 kb on - strand, within DDA3937_RS16865 at 3834.449 kb on - strand, within DDA3937_RS16865 at 3834.648 kb on + strand, within DDA3937_RS16865 at 3834.648 kb on + strand, within DDA3937_RS16865 at 3834.648 kb on + strand, within DDA3937_RS16865 at 3834.648 kb on + strand, within DDA3937_RS16865 at 3834.649 kb on - strand, within DDA3937_RS16865 at 3834.649 kb on - strand, within DDA3937_RS16865 at 3834.661 kb on + strand, within DDA3937_RS16865 at 3834.662 kb on - strand, within DDA3937_RS16865 at 3834.784 kb on + strand at 3834.789 kb on + strand at 3834.829 kb on + strand at 3834.833 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,832,634 + -1.3 3,832,634 + -1.6 3,832,635 - -3.1 3,832,638 + +1.3 3,832,648 + -0.6 3,832,648 + +3.1 3,832,661 - -0.8 3,832,681 - -0.4 3,832,681 - -0.6 3,832,689 - -3.0 3,832,689 - -1.6 3,832,689 - +1.6 3,832,711 + -0.3 3,832,712 - +0.6 3,832,712 - +0.1 3,832,712 - +0.5 3,832,793 - DDA3937_RS16855 0.11 -0.1 3,832,801 + DDA3937_RS16855 0.12 +0.7 3,832,872 - DDA3937_RS16855 0.21 -0.2 3,832,895 - DDA3937_RS16855 0.24 +3.2 3,832,907 + DDA3937_RS16855 0.25 -0.2 3,832,908 - DDA3937_RS16855 0.25 -0.2 3,832,908 - DDA3937_RS16855 0.25 -1.9 3,832,908 - DDA3937_RS16855 0.25 -0.6 3,832,908 - DDA3937_RS16855 0.25 +1.9 3,832,974 - DDA3937_RS16855 0.33 -0.6 3,833,003 + DDA3937_RS16855 0.37 -0.9 3,833,004 - +0.4 3,833,005 + DDA3937_RS16855 0.37 -0.5 3,833,005 + DDA3937_RS16855 0.37 +0.7 3,833,006 - DDA3937_RS16855 0.37 -0.5 3,833,006 - DDA3937_RS16855 0.37 +0.2 3,833,315 + DDA3937_RS16855 0.75 -0.5 3,833,315 + DDA3937_RS16855 0.75 +0.2 3,833,315 + DDA3937_RS16855 0.75 -1.9 3,833,315 + DDA3937_RS16855 0.75 -1.1 3,833,315 + DDA3937_RS16855 0.75 -1.6 3,833,315 + DDA3937_RS16855 0.75 -0.4 3,833,315 + DDA3937_RS16855 0.75 -1.9 3,833,315 + DDA3937_RS16855 0.75 -0.4 3,833,315 + DDA3937_RS16855 0.75 -0.2 3,833,315 + DDA3937_RS16855 0.75 +1.4 3,833,315 + DDA3937_RS16855 0.75 +1.5 3,833,315 + DDA3937_RS16855 0.75 +0.5 3,833,315 + DDA3937_RS16855 0.75 +0.0 3,833,315 + DDA3937_RS16855 0.75 +0.3 3,833,316 - DDA3937_RS16855 0.75 -2.2 3,833,316 - DDA3937_RS16855 0.75 -1.4 3,833,316 - DDA3937_RS16855 0.75 -0.2 3,833,316 - DDA3937_RS16855 0.75 -1.6 3,833,316 - DDA3937_RS16855 0.75 -2.2 3,833,316 - DDA3937_RS16855 0.75 -1.0 3,833,316 - DDA3937_RS16855 0.75 -1.1 3,833,316 - DDA3937_RS16855 0.75 -1.5 3,833,316 - DDA3937_RS16855 0.75 -0.2 3,833,381 + DDA3937_RS16855 0.83 -1.0 3,833,442 + +2.7 3,833,443 - -1.4 3,833,451 - -0.6 3,833,466 + -0.3 3,833,467 - +1.0 3,833,467 - -0.8 3,833,487 + -1.6 3,833,487 + -0.9 3,833,488 - -2.5 3,833,491 + -0.6 3,833,491 + -0.7 3,833,491 + -0.1 3,833,491 + -1.0 3,833,491 + +1.9 3,833,492 - -0.9 3,833,516 + +0.3 3,833,516 + +0.1 3,833,517 - -2.4 3,833,521 + -0.1 3,833,522 - -0.1 3,833,522 - -0.7 3,833,522 - +1.4 3,833,524 + -0.4 3,833,524 + -2.2 3,833,524 + -0.8 3,833,524 + -0.8 3,833,524 + -0.3 3,833,525 - -0.6 3,833,525 - -1.1 3,833,528 + +0.6 3,833,528 + -0.2 3,833,529 - +0.2 3,833,529 - -0.2 3,833,533 + +0.9 3,833,534 - -0.8 3,833,534 - +0.2 3,833,585 + +1.5 3,833,585 + -0.1 3,833,611 + -1.6 3,833,633 - -0.1 3,833,643 + -0.6 3,833,644 - -0.1 3,833,644 - +0.8 3,833,791 + DDA3937_RS16860 0.39 +0.5 3,833,792 - DDA3937_RS16860 0.39 +0.8 3,833,792 - DDA3937_RS16860 0.39 -1.9 3,833,812 - DDA3937_RS16860 0.44 +0.1 3,833,876 - DDA3937_RS16860 0.60 +3.4 3,833,974 + DDA3937_RS16860 0.84 -0.4 3,833,975 - DDA3937_RS16860 0.84 +0.9 3,833,975 - DDA3937_RS16860 0.84 +0.4 3,833,975 - DDA3937_RS16860 0.84 +1.9 3,833,990 + DDA3937_RS16860 0.88 -1.1 3,834,082 + +0.1 3,834,083 - -1.5 3,834,083 - -0.1 3,834,083 - +0.1 3,834,083 - +1.9 3,834,083 - +0.5 3,834,083 - -1.1 3,834,083 - +1.5 3,834,084 + +0.4 3,834,085 - -1.9 3,834,085 - -0.9 3,834,085 - -0.1 3,834,143 + DDA3937_RS16865 0.15 +0.0 3,834,143 + DDA3937_RS16865 0.15 +0.2 3,834,143 + DDA3937_RS16865 0.15 -0.5 3,834,143 + DDA3937_RS16865 0.15 -0.4 3,834,143 + DDA3937_RS16865 0.15 -0.9 3,834,143 + DDA3937_RS16865 0.15 +0.2 3,834,143 + DDA3937_RS16865 0.15 -0.8 3,834,143 + DDA3937_RS16865 0.15 +0.8 3,834,144 - DDA3937_RS16865 0.15 +1.5 3,834,144 - DDA3937_RS16865 0.15 +1.6 3,834,144 - DDA3937_RS16865 0.15 +0.4 3,834,144 - DDA3937_RS16865 0.15 -0.5 3,834,160 + DDA3937_RS16865 0.17 +0.8 3,834,160 + DDA3937_RS16865 0.17 -0.1 3,834,160 + DDA3937_RS16865 0.17 +0.1 3,834,161 - DDA3937_RS16865 0.18 +0.9 3,834,161 - DDA3937_RS16865 0.18 +0.3 3,834,161 - DDA3937_RS16865 0.18 -2.1 3,834,162 + DDA3937_RS16865 0.18 +0.6 3,834,162 + DDA3937_RS16865 0.18 -0.6 3,834,162 + DDA3937_RS16865 0.18 +1.9 3,834,162 + DDA3937_RS16865 0.18 +1.4 3,834,162 + DDA3937_RS16865 0.18 -0.1 3,834,163 - DDA3937_RS16865 0.18 +0.9 3,834,163 - DDA3937_RS16865 0.18 +0.5 3,834,163 - DDA3937_RS16865 0.18 -0.1 3,834,163 - DDA3937_RS16865 0.18 -1.4 3,834,339 + DDA3937_RS16865 0.43 -0.7 3,834,339 + DDA3937_RS16865 0.43 +0.7 3,834,339 + DDA3937_RS16865 0.43 +1.5 3,834,339 + DDA3937_RS16865 0.43 -0.4 3,834,339 + -0.1 3,834,340 - DDA3937_RS16865 0.43 -0.7 3,834,340 - DDA3937_RS16865 0.43 +0.0 3,834,340 - DDA3937_RS16865 0.43 +0.4 3,834,340 - DDA3937_RS16865 0.43 +1.5 3,834,416 + DDA3937_RS16865 0.54 -0.1 3,834,417 - DDA3937_RS16865 0.54 -0.5 3,834,449 - DDA3937_RS16865 0.58 -1.3 3,834,648 + DDA3937_RS16865 0.86 -0.3 3,834,648 + DDA3937_RS16865 0.86 -0.3 3,834,648 + DDA3937_RS16865 0.86 +0.4 3,834,648 + DDA3937_RS16865 0.86 +0.7 3,834,649 - DDA3937_RS16865 0.86 +0.3 3,834,649 - DDA3937_RS16865 0.86 -0.4 3,834,661 + DDA3937_RS16865 0.88 +0.6 3,834,662 - DDA3937_RS16865 0.88 -0.2 3,834,784 + -0.1 3,834,789 + -0.6 3,834,829 + -0.6 3,834,833 + -1.6
Or see this region's nucleotide sequence