Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS15945 and DDA3937_RS15950 overlap by 4 nucleotides DDA3937_RS15950 and DDA3937_RS15955 are separated by 160 nucleotides DDA3937_RS15955 and DDA3937_RS15960 are separated by 15 nucleotides DDA3937_RS15960 and DDA3937_RS15965 are separated by 25 nucleotides
DDA3937_RS15945: DDA3937_RS15945 - polynucleotide adenylyltransferase PcnB, at 3,648,570 to 3,649,973
_RS15945
DDA3937_RS15950: DDA3937_RS15950 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,649,970 to 3,650,458
_RS15950
DDA3937_RS15955: DDA3937_RS15955 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,650,619 to 3,651,413
_RS15955
DDA3937_RS15960: DDA3937_RS15960 - pantoate--beta-alanine ligase, at 3,651,429 to 3,652,283
_RS15960
DDA3937_RS15965: DDA3937_RS15965 - aspartate 1-decarboxylase, at 3,652,309 to 3,652,689
_RS15965
Position (kb)
3650
3651
3652 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3649.691 kb on + strand, within DDA3937_RS15945 at 3649.822 kb on + strand, within DDA3937_RS15945 at 3649.822 kb on + strand, within DDA3937_RS15945 at 3649.882 kb on + strand at 3649.882 kb on + strand at 3649.883 kb on - strand at 3649.883 kb on - strand at 3650.130 kb on - strand, within DDA3937_RS15950 at 3650.526 kb on + strand at 3650.532 kb on + strand at 3650.539 kb on - strand at 3650.569 kb on + strand at 3650.570 kb on - strand at 3650.570 kb on - strand at 3650.570 kb on - strand at 3650.634 kb on - strand at 3650.691 kb on + strand at 3650.691 kb on + strand at 3650.692 kb on - strand at 3650.713 kb on + strand, within DDA3937_RS15955 at 3650.714 kb on - strand, within DDA3937_RS15955 at 3650.762 kb on + strand, within DDA3937_RS15955 at 3650.770 kb on + strand, within DDA3937_RS15955 at 3650.810 kb on + strand, within DDA3937_RS15955 at 3650.879 kb on + strand, within DDA3937_RS15955 at 3650.880 kb on - strand, within DDA3937_RS15955 at 3650.942 kb on + strand, within DDA3937_RS15955 at 3650.942 kb on + strand, within DDA3937_RS15955 at 3650.943 kb on - strand, within DDA3937_RS15955 at 3650.943 kb on - strand, within DDA3937_RS15955 at 3650.945 kb on + strand at 3650.945 kb on + strand, within DDA3937_RS15955 at 3650.945 kb on + strand, within DDA3937_RS15955 at 3650.945 kb on + strand, within DDA3937_RS15955 at 3650.946 kb on - strand, within DDA3937_RS15955 at 3650.946 kb on - strand, within DDA3937_RS15955 at 3650.946 kb on - strand, within DDA3937_RS15955 at 3650.990 kb on + strand, within DDA3937_RS15955 at 3650.990 kb on + strand, within DDA3937_RS15955 at 3650.991 kb on - strand, within DDA3937_RS15955 at 3650.991 kb on - strand, within DDA3937_RS15955 at 3650.991 kb on - strand, within DDA3937_RS15955 at 3650.991 kb on - strand, within DDA3937_RS15955 at 3650.991 kb on - strand, within DDA3937_RS15955 at 3651.053 kb on + strand, within DDA3937_RS15955 at 3651.053 kb on + strand, within DDA3937_RS15955 at 3651.054 kb on - strand, within DDA3937_RS15955 at 3651.054 kb on - strand, within DDA3937_RS15955 at 3651.066 kb on + strand, within DDA3937_RS15955 at 3651.068 kb on + strand, within DDA3937_RS15955 at 3651.068 kb on + strand, within DDA3937_RS15955 at 3651.068 kb on + strand, within DDA3937_RS15955 at 3651.068 kb on + strand, within DDA3937_RS15955 at 3651.148 kb on + strand, within DDA3937_RS15955 at 3651.206 kb on + strand, within DDA3937_RS15955 at 3651.207 kb on - strand, within DDA3937_RS15955 at 3651.294 kb on - strand, within DDA3937_RS15955 at 3651.357 kb on + strand at 3651.357 kb on + strand at 3651.357 kb on + strand at 3651.357 kb on + strand at 3651.357 kb on + strand at 3651.358 kb on - strand at 3651.358 kb on - strand at 3651.358 kb on - strand at 3651.400 kb on - strand at 3651.400 kb on - strand at 3651.412 kb on - strand at 3651.723 kb on + strand, within DDA3937_RS15960 at 3651.760 kb on + strand, within DDA3937_RS15960 at 3651.930 kb on + strand, within DDA3937_RS15960 at 3651.931 kb on - strand, within DDA3937_RS15960 at 3652.063 kb on + strand, within DDA3937_RS15960 at 3652.063 kb on + strand, within DDA3937_RS15960 at 3652.064 kb on - strand, within DDA3937_RS15960 at 3652.064 kb on - strand, within DDA3937_RS15960 at 3652.100 kb on + strand, within DDA3937_RS15960 at 3652.100 kb on + strand, within DDA3937_RS15960 at 3652.101 kb on - strand, within DDA3937_RS15960 at 3652.101 kb on - strand, within DDA3937_RS15960 at 3652.101 kb on - strand, within DDA3937_RS15960 at 3652.101 kb on - strand, within DDA3937_RS15960 at 3652.101 kb on - strand, within DDA3937_RS15960 at 3652.101 kb on - strand, within DDA3937_RS15960 at 3652.156 kb on + strand, within DDA3937_RS15960 at 3652.156 kb on + strand, within DDA3937_RS15960 at 3652.156 kb on + strand, within DDA3937_RS15960 at 3652.157 kb on - strand at 3652.157 kb on - strand, within DDA3937_RS15960 at 3652.157 kb on - strand, within DDA3937_RS15960 at 3652.160 kb on + strand at 3652.160 kb on + strand, within DDA3937_RS15960 at 3652.161 kb on - strand, within DDA3937_RS15960 at 3652.180 kb on + strand, within DDA3937_RS15960 at 3652.180 kb on + strand, within DDA3937_RS15960 at 3652.181 kb on - strand, within DDA3937_RS15960 at 3652.332 kb on + strand at 3652.332 kb on + strand at 3652.332 kb on + strand at 3652.332 kb on + strand at 3652.332 kb on + strand at 3652.333 kb on - strand at 3652.333 kb on - strand at 3652.353 kb on - strand, within DDA3937_RS15965 at 3652.353 kb on - strand, within DDA3937_RS15965 at 3652.368 kb on - strand, within DDA3937_RS15965 at 3652.372 kb on + strand, within DDA3937_RS15965 at 3652.372 kb on + strand, within DDA3937_RS15965 at 3652.372 kb on + strand, within DDA3937_RS15965 at 3652.372 kb on + strand, within DDA3937_RS15965 at 3652.373 kb on - strand, within DDA3937_RS15965 at 3652.373 kb on - strand, within DDA3937_RS15965 at 3652.373 kb on - strand, within DDA3937_RS15965 at 3652.373 kb on - strand, within DDA3937_RS15965 at 3652.405 kb on - strand, within DDA3937_RS15965
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,649,691 + DDA3937_RS15945 0.80 -0.9 3,649,822 + DDA3937_RS15945 0.89 +0.7 3,649,822 + DDA3937_RS15945 0.89 -1.3 3,649,882 + +0.6 3,649,882 + +0.2 3,649,883 - -0.7 3,649,883 - +1.2 3,650,130 - DDA3937_RS15950 0.33 -1.4 3,650,526 + +1.3 3,650,532 + -0.3 3,650,539 - -0.9 3,650,569 + +1.1 3,650,570 - +1.3 3,650,570 - -3.3 3,650,570 - -2.2 3,650,634 - -1.1 3,650,691 + -2.1 3,650,691 + +1.1 3,650,692 - -1.4 3,650,713 + DDA3937_RS15955 0.12 -3.3 3,650,714 - DDA3937_RS15955 0.12 -0.7 3,650,762 + DDA3937_RS15955 0.18 -1.7 3,650,770 + DDA3937_RS15955 0.19 -2.7 3,650,810 + DDA3937_RS15955 0.24 -3.4 3,650,879 + DDA3937_RS15955 0.33 -1.7 3,650,880 - DDA3937_RS15955 0.33 -2.1 3,650,942 + DDA3937_RS15955 0.41 -2.8 3,650,942 + DDA3937_RS15955 0.41 -2.6 3,650,943 - DDA3937_RS15955 0.41 -3.9 3,650,943 - DDA3937_RS15955 0.41 -1.7 3,650,945 + -0.7 3,650,945 + DDA3937_RS15955 0.41 -2.3 3,650,945 + DDA3937_RS15955 0.41 -2.5 3,650,945 + DDA3937_RS15955 0.41 -2.3 3,650,946 - DDA3937_RS15955 0.41 -2.8 3,650,946 - DDA3937_RS15955 0.41 -2.6 3,650,946 - DDA3937_RS15955 0.41 -3.7 3,650,990 + DDA3937_RS15955 0.47 -1.6 3,650,990 + DDA3937_RS15955 0.47 -3.2 3,650,991 - DDA3937_RS15955 0.47 -2.9 3,650,991 - DDA3937_RS15955 0.47 -3.0 3,650,991 - DDA3937_RS15955 0.47 -3.5 3,650,991 - DDA3937_RS15955 0.47 -2.0 3,650,991 - DDA3937_RS15955 0.47 -1.7 3,651,053 + DDA3937_RS15955 0.55 -2.9 3,651,053 + DDA3937_RS15955 0.55 -2.6 3,651,054 - DDA3937_RS15955 0.55 -2.9 3,651,054 - DDA3937_RS15955 0.55 -1.8 3,651,066 + DDA3937_RS15955 0.56 -1.3 3,651,068 + DDA3937_RS15955 0.56 -2.1 3,651,068 + DDA3937_RS15955 0.56 -2.7 3,651,068 + DDA3937_RS15955 0.56 -3.4 3,651,068 + DDA3937_RS15955 0.56 -2.6 3,651,148 + DDA3937_RS15955 0.67 -1.4 3,651,206 + DDA3937_RS15955 0.74 -0.7 3,651,207 - DDA3937_RS15955 0.74 -2.3 3,651,294 - DDA3937_RS15955 0.85 -2.7 3,651,357 + -1.4 3,651,357 + -2.5 3,651,357 + -1.1 3,651,357 + -2.8 3,651,357 + -0.9 3,651,358 - -1.3 3,651,358 - -1.1 3,651,358 - -2.7 3,651,400 - +0.2 3,651,400 - -2.6 3,651,412 - -1.8 3,651,723 + DDA3937_RS15960 0.34 -3.3 3,651,760 + DDA3937_RS15960 0.39 -5.1 3,651,930 + DDA3937_RS15960 0.59 -0.7 3,651,931 - DDA3937_RS15960 0.59 -1.1 3,652,063 + DDA3937_RS15960 0.74 -0.1 3,652,063 + DDA3937_RS15960 0.74 -1.7 3,652,064 - DDA3937_RS15960 0.74 -2.6 3,652,064 - DDA3937_RS15960 0.74 -0.9 3,652,100 + DDA3937_RS15960 0.78 -2.0 3,652,100 + DDA3937_RS15960 0.78 -1.1 3,652,101 - DDA3937_RS15960 0.79 -3.6 3,652,101 - DDA3937_RS15960 0.79 -2.7 3,652,101 - DDA3937_RS15960 0.79 -0.1 3,652,101 - DDA3937_RS15960 0.79 -1.9 3,652,101 - DDA3937_RS15960 0.79 -2.8 3,652,101 - DDA3937_RS15960 0.79 -0.7 3,652,156 + DDA3937_RS15960 0.85 -1.6 3,652,156 + DDA3937_RS15960 0.85 -4.3 3,652,156 + DDA3937_RS15960 0.85 -3.8 3,652,157 - -0.7 3,652,157 - DDA3937_RS15960 0.85 -3.2 3,652,157 - DDA3937_RS15960 0.85 -3.3 3,652,160 + -1.1 3,652,160 + DDA3937_RS15960 0.85 -5.3 3,652,161 - DDA3937_RS15960 0.86 -1.4 3,652,180 + DDA3937_RS15960 0.88 -3.3 3,652,180 + DDA3937_RS15960 0.88 -3.5 3,652,181 - DDA3937_RS15960 0.88 -1.4 3,652,332 + -3.7 3,652,332 + -2.3 3,652,332 + -2.6 3,652,332 + -1.2 3,652,332 + -4.8 3,652,333 - -2.9 3,652,333 - -0.7 3,652,353 - DDA3937_RS15965 0.12 -2.8 3,652,353 - DDA3937_RS15965 0.12 -1.8 3,652,368 - DDA3937_RS15965 0.15 -5.1 3,652,372 + DDA3937_RS15965 0.17 -1.7 3,652,372 + DDA3937_RS15965 0.17 -1.2 3,652,372 + DDA3937_RS15965 0.17 -3.1 3,652,372 + DDA3937_RS15965 0.17 -2.8 3,652,373 - DDA3937_RS15965 0.17 -3.7 3,652,373 - DDA3937_RS15965 0.17 -3.1 3,652,373 - DDA3937_RS15965 0.17 -4.5 3,652,373 - DDA3937_RS15965 0.17 +0.5 3,652,405 - DDA3937_RS15965 0.25 -0.1
Or see this region's nucleotide sequence