Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS04025 and DDA3937_RS04030 overlap by 4 nucleotides DDA3937_RS04030 and DDA3937_RS04035 are separated by 2 nucleotides DDA3937_RS04035 and DDA3937_RS04040 are separated by 45 nucleotides DDA3937_RS04040 and DDA3937_RS04045 overlap by 4 nucleotides
DDA3937_RS04025: DDA3937_RS04025 - conjugal transfer protein TrbF, at 898,501 to 899,205
_RS04025
DDA3937_RS04030: DDA3937_RS04030 - P-type conjugative transfer protein TrbG, at 899,202 to 900,098
_RS04030
DDA3937_RS04035: DDA3937_RS04035 - TrbI/VirB10 family protein, at 900,101 to 901,339
_RS04035
DDA3937_RS04040: DDA3937_RS04040 - type II toxin-antitoxin system RelE/ParE family toxin, at 901,385 to 901,696
_RS04040
DDA3937_RS04045: DDA3937_RS04045 - DNA-damage-inducible protein J, at 901,693 to 901,977
_RS04045
Position (kb)
900
901
902 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 899.147 kb on - strand at 899.149 kb on - strand at 899.217 kb on + strand at 899.218 kb on - strand at 899.218 kb on - strand at 899.218 kb on - strand at 899.218 kb on - strand at 899.220 kb on + strand at 899.222 kb on - strand at 899.235 kb on + strand at 899.236 kb on - strand at 899.236 kb on - strand at 899.327 kb on + strand, within DDA3937_RS04030 at 899.327 kb on + strand, within DDA3937_RS04030 at 899.327 kb on + strand, within DDA3937_RS04030 at 899.344 kb on + strand, within DDA3937_RS04030 at 899.344 kb on + strand, within DDA3937_RS04030 at 899.469 kb on + strand, within DDA3937_RS04030 at 899.469 kb on + strand, within DDA3937_RS04030 at 899.469 kb on + strand, within DDA3937_RS04030 at 899.470 kb on - strand, within DDA3937_RS04030 at 899.470 kb on - strand, within DDA3937_RS04030 at 899.480 kb on + strand at 899.481 kb on - strand, within DDA3937_RS04030 at 899.481 kb on - strand, within DDA3937_RS04030 at 899.552 kb on + strand, within DDA3937_RS04030 at 899.576 kb on + strand, within DDA3937_RS04030 at 899.577 kb on - strand, within DDA3937_RS04030 at 899.613 kb on - strand, within DDA3937_RS04030 at 899.613 kb on - strand, within DDA3937_RS04030 at 899.679 kb on + strand, within DDA3937_RS04030 at 899.679 kb on + strand, within DDA3937_RS04030 at 899.679 kb on + strand, within DDA3937_RS04030 at 899.679 kb on + strand, within DDA3937_RS04030 at 899.680 kb on - strand, within DDA3937_RS04030 at 899.680 kb on - strand, within DDA3937_RS04030 at 899.680 kb on - strand, within DDA3937_RS04030 at 899.736 kb on + strand, within DDA3937_RS04030 at 899.892 kb on + strand, within DDA3937_RS04030 at 899.892 kb on + strand, within DDA3937_RS04030 at 899.892 kb on + strand, within DDA3937_RS04030 at 899.892 kb on + strand, within DDA3937_RS04030 at 899.893 kb on - strand, within DDA3937_RS04030 at 899.893 kb on - strand, within DDA3937_RS04030 at 899.893 kb on - strand, within DDA3937_RS04030 at 899.893 kb on - strand, within DDA3937_RS04030 at 899.893 kb on - strand, within DDA3937_RS04030 at 899.980 kb on - strand, within DDA3937_RS04030 at 899.997 kb on + strand, within DDA3937_RS04030 at 899.997 kb on + strand, within DDA3937_RS04030 at 899.997 kb on + strand, within DDA3937_RS04030 at 899.998 kb on - strand, within DDA3937_RS04030 at 899.998 kb on - strand, within DDA3937_RS04030 at 899.998 kb on - strand at 900.000 kb on + strand, within DDA3937_RS04030 at 900.000 kb on + strand, within DDA3937_RS04030 at 900.000 kb on + strand, within DDA3937_RS04030 at 900.000 kb on + strand, within DDA3937_RS04030 at 900.000 kb on + strand at 900.000 kb on + strand, within DDA3937_RS04030 at 900.000 kb on + strand, within DDA3937_RS04030 at 900.000 kb on + strand, within DDA3937_RS04030 at 900.001 kb on - strand, within DDA3937_RS04030 at 900.001 kb on - strand, within DDA3937_RS04030 at 900.001 kb on - strand, within DDA3937_RS04030 at 900.001 kb on - strand, within DDA3937_RS04030 at 900.001 kb on - strand, within DDA3937_RS04030 at 900.001 kb on - strand, within DDA3937_RS04030 at 900.001 kb on - strand, within DDA3937_RS04030 at 900.001 kb on - strand, within DDA3937_RS04030 at 900.001 kb on - strand, within DDA3937_RS04030 at 900.001 kb on - strand, within DDA3937_RS04030 at 900.072 kb on - strand at 900.100 kb on + strand at 900.100 kb on + strand at 900.101 kb on - strand at 900.101 kb on - strand at 900.101 kb on - strand at 900.101 kb on - strand at 900.273 kb on + strand, within DDA3937_RS04035 at 900.274 kb on - strand, within DDA3937_RS04035 at 900.274 kb on - strand, within DDA3937_RS04035 at 900.274 kb on - strand, within DDA3937_RS04035 at 900.274 kb on - strand, within DDA3937_RS04035 at 900.296 kb on - strand, within DDA3937_RS04035 at 900.317 kb on + strand, within DDA3937_RS04035 at 900.318 kb on - strand, within DDA3937_RS04035 at 900.318 kb on - strand, within DDA3937_RS04035 at 900.318 kb on - strand, within DDA3937_RS04035 at 900.318 kb on - strand, within DDA3937_RS04035 at 900.318 kb on - strand, within DDA3937_RS04035 at 900.319 kb on + strand, within DDA3937_RS04035 at 900.319 kb on + strand, within DDA3937_RS04035 at 900.320 kb on - strand, within DDA3937_RS04035 at 900.371 kb on + strand, within DDA3937_RS04035 at 900.371 kb on + strand, within DDA3937_RS04035 at 900.403 kb on - strand, within DDA3937_RS04035 at 900.494 kb on + strand, within DDA3937_RS04035 at 900.495 kb on - strand, within DDA3937_RS04035 at 900.632 kb on - strand, within DDA3937_RS04035 at 900.794 kb on - strand, within DDA3937_RS04035 at 900.905 kb on + strand, within DDA3937_RS04035 at 900.905 kb on + strand, within DDA3937_RS04035 at 900.906 kb on - strand, within DDA3937_RS04035 at 900.906 kb on - strand, within DDA3937_RS04035 at 900.924 kb on - strand, within DDA3937_RS04035 at 901.022 kb on + strand, within DDA3937_RS04035 at 901.092 kb on + strand, within DDA3937_RS04035 at 901.092 kb on + strand, within DDA3937_RS04035 at 901.093 kb on - strand at 901.093 kb on - strand, within DDA3937_RS04035 at 901.147 kb on + strand, within DDA3937_RS04035 at 901.147 kb on + strand, within DDA3937_RS04035 at 901.147 kb on + strand, within DDA3937_RS04035 at 901.150 kb on + strand, within DDA3937_RS04035 at 901.151 kb on - strand, within DDA3937_RS04035 at 901.151 kb on - strand, within DDA3937_RS04035 at 901.151 kb on - strand, within DDA3937_RS04035 at 901.267 kb on + strand at 901.293 kb on + strand at 901.293 kb on + strand at 901.431 kb on - strand, within DDA3937_RS04040 at 901.441 kb on + strand, within DDA3937_RS04040 at 901.442 kb on - strand, within DDA3937_RS04040 at 901.442 kb on - strand, within DDA3937_RS04040 at 901.442 kb on - strand, within DDA3937_RS04040 at 901.442 kb on - strand, within DDA3937_RS04040 at 901.442 kb on - strand, within DDA3937_RS04040 at 901.442 kb on - strand, within DDA3937_RS04040 at 901.442 kb on - strand, within DDA3937_RS04040 at 901.442 kb on - strand, within DDA3937_RS04040 at 901.442 kb on - strand, within DDA3937_RS04040 at 901.445 kb on + strand, within DDA3937_RS04040 at 901.446 kb on - strand, within DDA3937_RS04040 at 901.446 kb on - strand, within DDA3937_RS04040 at 901.446 kb on - strand, within DDA3937_RS04040 at 901.473 kb on + strand, within DDA3937_RS04040 at 901.473 kb on + strand, within DDA3937_RS04040 at 901.483 kb on + strand, within DDA3937_RS04040 at 901.664 kb on - strand, within DDA3937_RS04040 at 901.929 kb on + strand at 901.966 kb on + strand at 901.967 kb on - strand at 901.967 kb on - strand at 901.967 kb on - strand at 901.967 kb on - strand at 901.967 kb on - strand at 901.967 kb on - strand at 901.967 kb on - strand at 901.967 kb on - strand at 901.967 kb on - strand at 901.990 kb on + strand at 902.026 kb on + strand at 902.079 kb on + strand at 902.080 kb on - strand at 902.134 kb on + strand at 902.134 kb on + strand at 902.135 kb on - strand at 902.135 kb on - strand at 902.135 kb on - strand at 902.137 kb on + strand at 902.138 kb on - strand at 902.162 kb on + strand at 902.162 kb on + strand at 902.181 kb on + strand at 902.181 kb on + strand at 902.221 kb on + strand at 902.221 kb on + strand at 902.227 kb on + strand at 902.235 kb on + strand at 902.235 kb on + strand at 902.236 kb on - strand at 902.239 kb on + strand at 902.239 kb on + strand at 902.239 kb on + strand at 902.240 kb on - strand at 902.240 kb on - strand at 902.240 kb on - strand at 902.240 kb on - strand at 902.242 kb on - strand at 902.245 kb on + strand at 902.245 kb on + strand at 902.249 kb on + strand at 902.261 kb on - strand at 902.296 kb on - strand at 902.296 kb on - strand at 902.298 kb on - strand at 902.298 kb on - strand at 902.298 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 899,147 - +0.6 899,149 - +1.4 899,217 + -1.5 899,218 - -0.8 899,218 - +0.2 899,218 - -0.0 899,218 - +0.7 899,220 + -0.1 899,222 - +1.2 899,235 + -1.7 899,236 - -2.5 899,236 - -1.6 899,327 + DDA3937_RS04030 0.14 -1.2 899,327 + DDA3937_RS04030 0.14 -0.9 899,327 + DDA3937_RS04030 0.14 -1.0 899,344 + DDA3937_RS04030 0.16 +0.4 899,344 + DDA3937_RS04030 0.16 -0.8 899,469 + DDA3937_RS04030 0.30 -0.4 899,469 + DDA3937_RS04030 0.30 -0.9 899,469 + DDA3937_RS04030 0.30 -1.7 899,470 - DDA3937_RS04030 0.30 +0.6 899,470 - DDA3937_RS04030 0.30 +0.3 899,480 + -0.0 899,481 - DDA3937_RS04030 0.31 +1.1 899,481 - DDA3937_RS04030 0.31 +1.9 899,552 + DDA3937_RS04030 0.39 -0.0 899,576 + DDA3937_RS04030 0.42 +0.3 899,577 - DDA3937_RS04030 0.42 -0.8 899,613 - DDA3937_RS04030 0.46 +0.1 899,613 - DDA3937_RS04030 0.46 +1.9 899,679 + DDA3937_RS04030 0.53 -1.4 899,679 + DDA3937_RS04030 0.53 +0.1 899,679 + DDA3937_RS04030 0.53 -2.5 899,679 + DDA3937_RS04030 0.53 +1.6 899,680 - DDA3937_RS04030 0.53 -0.0 899,680 - DDA3937_RS04030 0.53 +4.2 899,680 - DDA3937_RS04030 0.53 +4.4 899,736 + DDA3937_RS04030 0.60 -1.3 899,892 + DDA3937_RS04030 0.77 -0.8 899,892 + DDA3937_RS04030 0.77 +1.1 899,892 + DDA3937_RS04030 0.77 -0.6 899,892 + DDA3937_RS04030 0.77 +2.2 899,893 - DDA3937_RS04030 0.77 +0.3 899,893 - DDA3937_RS04030 0.77 +0.4 899,893 - DDA3937_RS04030 0.77 -1.8 899,893 - DDA3937_RS04030 0.77 -0.7 899,893 - DDA3937_RS04030 0.77 -0.7 899,980 - DDA3937_RS04030 0.87 +0.4 899,997 + DDA3937_RS04030 0.89 -0.5 899,997 + DDA3937_RS04030 0.89 -2.2 899,997 + DDA3937_RS04030 0.89 -0.3 899,998 - DDA3937_RS04030 0.89 +1.0 899,998 - DDA3937_RS04030 0.89 +0.3 899,998 - -1.3 900,000 + DDA3937_RS04030 0.89 -0.2 900,000 + DDA3937_RS04030 0.89 +3.0 900,000 + DDA3937_RS04030 0.89 -0.5 900,000 + DDA3937_RS04030 0.89 -1.3 900,000 + -0.6 900,000 + DDA3937_RS04030 0.89 +0.5 900,000 + DDA3937_RS04030 0.89 -3.8 900,000 + DDA3937_RS04030 0.89 -1.3 900,001 - DDA3937_RS04030 0.89 -0.1 900,001 - DDA3937_RS04030 0.89 +0.2 900,001 - DDA3937_RS04030 0.89 -0.5 900,001 - DDA3937_RS04030 0.89 +1.6 900,001 - DDA3937_RS04030 0.89 -1.7 900,001 - DDA3937_RS04030 0.89 +3.3 900,001 - DDA3937_RS04030 0.89 +0.2 900,001 - DDA3937_RS04030 0.89 -0.4 900,001 - DDA3937_RS04030 0.89 +0.6 900,001 - DDA3937_RS04030 0.89 +0.3 900,072 - +0.4 900,100 + -0.4 900,100 + -1.0 900,101 - +1.6 900,101 - -0.3 900,101 - +1.0 900,101 - -2.2 900,273 + DDA3937_RS04035 0.14 -1.3 900,274 - DDA3937_RS04035 0.14 +0.7 900,274 - DDA3937_RS04035 0.14 -0.3 900,274 - DDA3937_RS04035 0.14 -1.6 900,274 - DDA3937_RS04035 0.14 -0.3 900,296 - DDA3937_RS04035 0.16 +0.3 900,317 + DDA3937_RS04035 0.17 -1.0 900,318 - DDA3937_RS04035 0.18 +0.6 900,318 - DDA3937_RS04035 0.18 +3.1 900,318 - DDA3937_RS04035 0.18 +1.8 900,318 - DDA3937_RS04035 0.18 +0.7 900,318 - DDA3937_RS04035 0.18 -0.6 900,319 + DDA3937_RS04035 0.18 -0.0 900,319 + DDA3937_RS04035 0.18 -0.4 900,320 - DDA3937_RS04035 0.18 -0.9 900,371 + DDA3937_RS04035 0.22 +1.2 900,371 + DDA3937_RS04035 0.22 -0.3 900,403 - DDA3937_RS04035 0.24 +0.6 900,494 + DDA3937_RS04035 0.32 -2.4 900,495 - DDA3937_RS04035 0.32 +0.5 900,632 - DDA3937_RS04035 0.43 +0.3 900,794 - DDA3937_RS04035 0.56 -0.8 900,905 + DDA3937_RS04035 0.65 -0.0 900,905 + DDA3937_RS04035 0.65 +0.1 900,906 - DDA3937_RS04035 0.65 -0.9 900,906 - DDA3937_RS04035 0.65 -1.3 900,924 - DDA3937_RS04035 0.66 -2.0 901,022 + DDA3937_RS04035 0.74 -1.3 901,092 + DDA3937_RS04035 0.80 +1.3 901,092 + DDA3937_RS04035 0.80 +0.6 901,093 - +2.0 901,093 - DDA3937_RS04035 0.80 -1.5 901,147 + DDA3937_RS04035 0.84 +0.3 901,147 + DDA3937_RS04035 0.84 -1.2 901,147 + DDA3937_RS04035 0.84 -1.2 901,150 + DDA3937_RS04035 0.85 -1.3 901,151 - DDA3937_RS04035 0.85 +0.8 901,151 - DDA3937_RS04035 0.85 +2.3 901,151 - DDA3937_RS04035 0.85 +0.3 901,267 + -0.0 901,293 + +0.0 901,293 + +0.3 901,431 - DDA3937_RS04040 0.15 +0.2 901,441 + DDA3937_RS04040 0.18 +2.4 901,442 - DDA3937_RS04040 0.18 -0.2 901,442 - DDA3937_RS04040 0.18 +2.0 901,442 - DDA3937_RS04040 0.18 -0.7 901,442 - DDA3937_RS04040 0.18 +0.2 901,442 - DDA3937_RS04040 0.18 -2.5 901,442 - DDA3937_RS04040 0.18 +0.7 901,442 - DDA3937_RS04040 0.18 -0.4 901,442 - DDA3937_RS04040 0.18 -0.7 901,442 - DDA3937_RS04040 0.18 -0.6 901,445 + DDA3937_RS04040 0.19 -0.6 901,446 - DDA3937_RS04040 0.20 +0.2 901,446 - DDA3937_RS04040 0.20 -0.1 901,446 - DDA3937_RS04040 0.20 -0.6 901,473 + DDA3937_RS04040 0.28 +1.7 901,473 + DDA3937_RS04040 0.28 +0.6 901,483 + DDA3937_RS04040 0.31 +0.1 901,664 - DDA3937_RS04040 0.89 -0.7 901,929 + +2.0 901,966 + +0.4 901,967 - -0.6 901,967 - +1.3 901,967 - -0.4 901,967 - -2.0 901,967 - -1.3 901,967 - -0.5 901,967 - +0.5 901,967 - -1.3 901,967 - +1.2 901,990 + -0.1 902,026 + -0.2 902,079 + -0.1 902,080 - -0.0 902,134 + +0.5 902,134 + -0.8 902,135 - -0.0 902,135 - +0.9 902,135 - -2.3 902,137 + -0.3 902,138 - +0.7 902,162 + -0.8 902,162 + +0.4 902,181 + +0.5 902,181 + -0.2 902,221 + -0.7 902,221 + -2.3 902,227 + -0.3 902,235 + -2.5 902,235 + +0.1 902,236 - +0.8 902,239 + -1.5 902,239 + +1.0 902,239 + -1.5 902,240 - -0.2 902,240 - -1.3 902,240 - -1.5 902,240 - -0.0 902,242 - -1.4 902,245 + +0.6 902,245 + -0.0 902,249 + -0.7 902,261 - +1.0 902,296 - -1.4 902,296 - +0.1 902,298 - +0.6 902,298 - +0.4 902,298 - -0.6
Or see this region's nucleotide sequence