Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS01530 and DDA3937_RS01535 are separated by 47 nucleotides DDA3937_RS01535 and DDA3937_RS01540 are separated by 15 nucleotides DDA3937_RS01540 and DDA3937_RS01545 overlap by 23 nucleotides
DDA3937_RS01530: DDA3937_RS01530 - RNA polymerase factor sigma-54, at 340,941 to 342,374
_RS01530
DDA3937_RS01535: DDA3937_RS01535 - LPS export ABC transporter ATP-binding protein, at 342,422 to 343,147
_RS01535
DDA3937_RS01540: DDA3937_RS01540 - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 343,163 to 343,741
_RS01540
DDA3937_RS01545: DDA3937_RS01545 - LPS export ABC transporter periplasmic protein LptC, at 343,719 to 344,288
_RS01545
Position (kb) 342
343
344 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 341.467 kb on + strand, within DDA3937_RS01530 at 341.504 kb on + strand, within DDA3937_RS01530 at 341.507 kb on + strand, within DDA3937_RS01530 at 341.563 kb on + strand, within DDA3937_RS01530 at 341.563 kb on + strand, within DDA3937_RS01530 at 341.564 kb on - strand at 341.652 kb on - strand, within DDA3937_RS01530 at 341.829 kb on + strand, within DDA3937_RS01530 at 341.830 kb on - strand, within DDA3937_RS01530 at 342.007 kb on + strand at 342.007 kb on + strand, within DDA3937_RS01530 at 342.040 kb on + strand, within DDA3937_RS01530 at 342.040 kb on + strand, within DDA3937_RS01530 at 342.143 kb on + strand, within DDA3937_RS01530 at 342.143 kb on + strand, within DDA3937_RS01530 at 342.144 kb on - strand, within DDA3937_RS01530 at 342.318 kb on + strand at 342.319 kb on - strand at 342.374 kb on + strand at 342.409 kb on + strand at 342.409 kb on + strand at 342.409 kb on + strand at 342.410 kb on - strand at 342.420 kb on + strand at 343.879 kb on - strand, within DDA3937_RS01545
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 341,467 + DDA3937_RS01530 0.37 -1.1 341,504 + DDA3937_RS01530 0.39 -1.8 341,507 + DDA3937_RS01530 0.39 -0.8 341,563 + DDA3937_RS01530 0.43 -2.3 341,563 + DDA3937_RS01530 0.43 -1.1 341,564 - -1.1 341,652 - DDA3937_RS01530 0.50 -0.8 341,829 + DDA3937_RS01530 0.62 -1.6 341,830 - DDA3937_RS01530 0.62 +0.9 342,007 + -0.4 342,007 + DDA3937_RS01530 0.74 -2.0 342,040 + DDA3937_RS01530 0.77 -2.8 342,040 + DDA3937_RS01530 0.77 -0.9 342,143 + DDA3937_RS01530 0.84 -1.8 342,143 + DDA3937_RS01530 0.84 -1.8 342,144 - DDA3937_RS01530 0.84 -2.3 342,318 + -2.4 342,319 - -2.0 342,374 + +1.7 342,409 + +0.4 342,409 + +0.5 342,409 + +0.0 342,410 - +1.8 342,420 + +1.3 343,879 - DDA3937_RS01545 0.28 +0.7
Or see this region's nucleotide sequence