Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS03225 and DDA3937_RS03230 are separated by 111 nucleotides DDA3937_RS03230 and DDA3937_RS03235 are separated by 55 nucleotides DDA3937_RS03235 and DDA3937_RS03240 are separated by 326 nucleotides
DDA3937_RS03225: DDA3937_RS03225 - LysR family transcriptional regulator, at 703,631 to 704,524
_RS03225
DDA3937_RS03230: DDA3937_RS03230 - acetolactate decarboxylase, at 704,636 to 705,424
_RS03230
DDA3937_RS03235: DDA3937_RS03235 - acetolactate synthase AlsS, at 705,480 to 707,159
_RS03235
DDA3937_RS03240: DDA3937_RS03240 - lactaldehyde reductase, at 707,486 to 708,637
_RS03240
Position (kb) 705
706
707
708 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 704.516 kb on + strand at 704.516 kb on + strand at 704.516 kb on + strand at 704.516 kb on + strand at 704.516 kb on + strand at 704.517 kb on - strand at 704.517 kb on - strand at 704.524 kb on + strand at 704.525 kb on - strand at 704.527 kb on - strand at 704.557 kb on + strand at 704.653 kb on + strand at 704.653 kb on + strand at 704.654 kb on - strand at 704.728 kb on + strand, within DDA3937_RS03230 at 704.728 kb on + strand, within DDA3937_RS03230 at 704.728 kb on + strand at 704.729 kb on - strand, within DDA3937_RS03230 at 704.761 kb on + strand, within DDA3937_RS03230 at 704.779 kb on + strand, within DDA3937_RS03230 at 704.779 kb on + strand, within DDA3937_RS03230 at 704.779 kb on + strand, within DDA3937_RS03230 at 704.779 kb on + strand, within DDA3937_RS03230 at 705.037 kb on + strand, within DDA3937_RS03230 at 705.037 kb on + strand, within DDA3937_RS03230 at 705.037 kb on + strand, within DDA3937_RS03230 at 705.037 kb on + strand, within DDA3937_RS03230 at 705.038 kb on - strand, within DDA3937_RS03230 at 705.060 kb on + strand, within DDA3937_RS03230 at 705.061 kb on - strand, within DDA3937_RS03230 at 705.061 kb on - strand, within DDA3937_RS03230 at 705.061 kb on - strand, within DDA3937_RS03230 at 705.074 kb on - strand, within DDA3937_RS03230 at 705.097 kb on - strand, within DDA3937_RS03230 at 705.097 kb on - strand, within DDA3937_RS03230 at 705.116 kb on + strand, within DDA3937_RS03230 at 705.116 kb on + strand, within DDA3937_RS03230 at 705.117 kb on - strand, within DDA3937_RS03230 at 705.117 kb on - strand, within DDA3937_RS03230 at 705.118 kb on + strand, within DDA3937_RS03230 at 705.119 kb on - strand, within DDA3937_RS03230 at 705.119 kb on - strand, within DDA3937_RS03230 at 705.119 kb on - strand, within DDA3937_RS03230 at 705.197 kb on - strand, within DDA3937_RS03230 at 705.206 kb on + strand, within DDA3937_RS03230 at 705.206 kb on + strand, within DDA3937_RS03230 at 705.206 kb on + strand, within DDA3937_RS03230 at 705.206 kb on + strand, within DDA3937_RS03230 at 705.206 kb on + strand, within DDA3937_RS03230 at 705.206 kb on + strand, within DDA3937_RS03230 at 705.207 kb on - strand, within DDA3937_RS03230 at 705.207 kb on - strand, within DDA3937_RS03230 at 705.207 kb on - strand, within DDA3937_RS03230 at 705.207 kb on - strand at 705.207 kb on - strand, within DDA3937_RS03230 at 705.234 kb on - strand, within DDA3937_RS03230 at 705.277 kb on + strand, within DDA3937_RS03230 at 705.277 kb on + strand, within DDA3937_RS03230 at 705.277 kb on + strand, within DDA3937_RS03230 at 705.277 kb on + strand, within DDA3937_RS03230 at 705.277 kb on + strand, within DDA3937_RS03230 at 705.277 kb on + strand, within DDA3937_RS03230 at 705.277 kb on + strand, within DDA3937_RS03230 at 705.278 kb on - strand, within DDA3937_RS03230 at 705.278 kb on - strand, within DDA3937_RS03230 at 705.278 kb on - strand, within DDA3937_RS03230 at 705.278 kb on - strand, within DDA3937_RS03230 at 705.278 kb on - strand, within DDA3937_RS03230 at 705.278 kb on - strand, within DDA3937_RS03230 at 705.287 kb on + strand, within DDA3937_RS03230 at 705.306 kb on + strand, within DDA3937_RS03230 at 705.306 kb on + strand, within DDA3937_RS03230 at 705.306 kb on + strand, within DDA3937_RS03230 at 705.307 kb on - strand, within DDA3937_RS03230 at 705.307 kb on - strand, within DDA3937_RS03230 at 705.307 kb on - strand, within DDA3937_RS03230 at 705.307 kb on - strand, within DDA3937_RS03230 at 705.378 kb on + strand at 705.379 kb on - strand at 705.438 kb on + strand at 705.461 kb on + strand at 705.461 kb on + strand at 705.462 kb on - strand at 705.472 kb on + strand at 705.488 kb on + strand at 705.488 kb on + strand at 705.488 kb on + strand at 705.489 kb on - strand at 705.513 kb on - strand at 705.532 kb on + strand at 705.532 kb on + strand at 705.561 kb on + strand at 705.561 kb on + strand at 705.561 kb on + strand at 705.561 kb on + strand at 705.562 kb on - strand at 705.562 kb on - strand at 705.562 kb on - strand at 705.562 kb on - strand at 705.562 kb on - strand at 705.562 kb on - strand at 705.562 kb on - strand at 705.562 kb on - strand at 705.562 kb on - strand at 705.590 kb on - strand at 705.665 kb on + strand, within DDA3937_RS03235 at 705.665 kb on + strand, within DDA3937_RS03235 at 705.880 kb on - strand, within DDA3937_RS03235 at 705.880 kb on - strand, within DDA3937_RS03235 at 705.880 kb on - strand, within DDA3937_RS03235 at 705.989 kb on + strand, within DDA3937_RS03235 at 706.091 kb on - strand, within DDA3937_RS03235 at 706.199 kb on + strand, within DDA3937_RS03235 at 706.199 kb on + strand, within DDA3937_RS03235 at 706.199 kb on + strand, within DDA3937_RS03235 at 706.200 kb on - strand, within DDA3937_RS03235 at 706.203 kb on + strand, within DDA3937_RS03235 at 706.203 kb on + strand, within DDA3937_RS03235 at 706.203 kb on + strand, within DDA3937_RS03235 at 706.204 kb on - strand, within DDA3937_RS03235 at 706.294 kb on + strand, within DDA3937_RS03235 at 706.294 kb on + strand, within DDA3937_RS03235 at 706.294 kb on + strand, within DDA3937_RS03235 at 706.294 kb on + strand, within DDA3937_RS03235 at 706.295 kb on - strand, within DDA3937_RS03235 at 706.295 kb on - strand, within DDA3937_RS03235 at 706.295 kb on - strand, within DDA3937_RS03235 at 706.311 kb on - strand, within DDA3937_RS03235 at 706.341 kb on + strand, within DDA3937_RS03235 at 706.341 kb on + strand, within DDA3937_RS03235 at 706.385 kb on + strand, within DDA3937_RS03235 at 706.385 kb on + strand, within DDA3937_RS03235 at 706.385 kb on + strand, within DDA3937_RS03235 at 706.386 kb on - strand, within DDA3937_RS03235 at 706.634 kb on + strand, within DDA3937_RS03235 at 706.635 kb on - strand, within DDA3937_RS03235 at 706.643 kb on + strand, within DDA3937_RS03235 at 706.644 kb on - strand, within DDA3937_RS03235 at 706.644 kb on - strand, within DDA3937_RS03235 at 706.644 kb on - strand, within DDA3937_RS03235 at 706.695 kb on + strand, within DDA3937_RS03235 at 706.908 kb on + strand, within DDA3937_RS03235 at 706.909 kb on - strand, within DDA3937_RS03235 at 706.982 kb on + strand, within DDA3937_RS03235 at 706.982 kb on + strand, within DDA3937_RS03235 at 707.009 kb on + strand at 707.121 kb on + strand at 707.121 kb on + strand at 707.122 kb on - strand at 707.163 kb on + strand at 707.163 kb on + strand at 707.164 kb on - strand at 707.164 kb on - strand at 707.166 kb on + strand at 707.167 kb on - strand at 707.184 kb on + strand at 707.185 kb on - strand at 707.185 kb on - strand at 707.192 kb on + strand at 707.226 kb on + strand at 707.244 kb on - strand at 707.244 kb on - strand at 707.254 kb on - strand at 707.263 kb on - strand at 707.263 kb on - strand at 707.328 kb on + strand at 707.328 kb on + strand at 707.328 kb on + strand at 707.328 kb on + strand at 707.328 kb on + strand at 707.328 kb on + strand at 707.328 kb on + strand at 707.329 kb on - strand at 707.329 kb on - strand at 707.329 kb on - strand at 707.329 kb on - strand at 707.331 kb on + strand at 707.454 kb on + strand at 707.460 kb on + strand at 707.460 kb on + strand at 707.461 kb on - strand at 707.461 kb on - strand at 707.461 kb on - strand at 707.464 kb on + strand at 707.464 kb on + strand at 707.464 kb on + strand at 707.464 kb on + strand at 707.464 kb on + strand at 707.464 kb on + strand at 707.464 kb on + strand at 707.465 kb on - strand at 707.465 kb on - strand at 707.465 kb on - strand at 707.465 kb on - strand at 707.465 kb on - strand at 707.465 kb on - strand at 707.490 kb on + strand at 707.490 kb on + strand at 707.490 kb on + strand at 707.491 kb on - strand at 707.491 kb on - strand at 707.491 kb on - strand at 707.491 kb on - strand at 707.502 kb on + strand at 707.502 kb on + strand at 707.502 kb on + strand at 707.503 kb on - strand at 707.503 kb on - strand at 707.658 kb on - strand, within DDA3937_RS03240 at 707.714 kb on + strand, within DDA3937_RS03240 at 707.714 kb on + strand at 707.757 kb on + strand, within DDA3937_RS03240 at 707.757 kb on + strand, within DDA3937_RS03240 at 707.821 kb on + strand, within DDA3937_RS03240 at 707.822 kb on - strand, within DDA3937_RS03240 at 707.823 kb on + strand, within DDA3937_RS03240 at 707.823 kb on + strand, within DDA3937_RS03240 at 707.823 kb on + strand, within DDA3937_RS03240 at 707.823 kb on + strand, within DDA3937_RS03240 at 707.823 kb on + strand, within DDA3937_RS03240 at 707.824 kb on - strand, within DDA3937_RS03240 at 707.824 kb on - strand, within DDA3937_RS03240 at 707.824 kb on - strand, within DDA3937_RS03240 at 707.824 kb on - strand, within DDA3937_RS03240 at 707.824 kb on - strand, within DDA3937_RS03240 at 707.824 kb on - strand, within DDA3937_RS03240 at 707.824 kb on - strand, within DDA3937_RS03240 at 707.824 kb on - strand, within DDA3937_RS03240 at 707.896 kb on + strand at 707.896 kb on + strand, within DDA3937_RS03240 at 707.897 kb on - strand, within DDA3937_RS03240 at 707.897 kb on - strand, within DDA3937_RS03240 at 707.898 kb on + strand, within DDA3937_RS03240 at 707.898 kb on + strand, within DDA3937_RS03240 at 707.898 kb on + strand, within DDA3937_RS03240 at 707.898 kb on + strand, within DDA3937_RS03240 at 707.898 kb on + strand, within DDA3937_RS03240 at 707.898 kb on + strand, within DDA3937_RS03240 at 707.898 kb on + strand, within DDA3937_RS03240 at 707.899 kb on - strand, within DDA3937_RS03240 at 707.899 kb on - strand, within DDA3937_RS03240 at 707.899 kb on - strand, within DDA3937_RS03240 at 707.899 kb on - strand, within DDA3937_RS03240 at 707.899 kb on - strand, within DDA3937_RS03240 at 707.899 kb on - strand, within DDA3937_RS03240 at 708.003 kb on - strand, within DDA3937_RS03240 at 708.026 kb on - strand, within DDA3937_RS03240 at 708.131 kb on - strand, within DDA3937_RS03240
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 704,516 + +1.7 704,516 + +0.5 704,516 + +1.7 704,516 + -0.2 704,516 + -1.2 704,517 - +0.3 704,517 - -0.7 704,524 + -0.3 704,525 - +2.1 704,527 - -2.0 704,557 + -1.8 704,653 + -0.1 704,653 + -1.6 704,654 - +0.3 704,728 + DDA3937_RS03230 0.12 +0.3 704,728 + DDA3937_RS03230 0.12 +0.0 704,728 + +2.2 704,729 - DDA3937_RS03230 0.12 +0.2 704,761 + DDA3937_RS03230 0.16 -2.4 704,779 + DDA3937_RS03230 0.18 -3.6 704,779 + DDA3937_RS03230 0.18 -0.2 704,779 + DDA3937_RS03230 0.18 -0.2 704,779 + DDA3937_RS03230 0.18 +0.8 705,037 + DDA3937_RS03230 0.51 -0.2 705,037 + DDA3937_RS03230 0.51 -0.7 705,037 + DDA3937_RS03230 0.51 +0.8 705,037 + DDA3937_RS03230 0.51 -1.1 705,038 - DDA3937_RS03230 0.51 -0.6 705,060 + DDA3937_RS03230 0.54 -1.7 705,061 - DDA3937_RS03230 0.54 +0.1 705,061 - DDA3937_RS03230 0.54 -0.3 705,061 - DDA3937_RS03230 0.54 +1.2 705,074 - DDA3937_RS03230 0.56 -2.2 705,097 - DDA3937_RS03230 0.58 +0.6 705,097 - DDA3937_RS03230 0.58 +0.3 705,116 + DDA3937_RS03230 0.61 -1.0 705,116 + DDA3937_RS03230 0.61 -1.6 705,117 - DDA3937_RS03230 0.61 -0.4 705,117 - DDA3937_RS03230 0.61 -1.1 705,118 + DDA3937_RS03230 0.61 -1.2 705,119 - DDA3937_RS03230 0.61 -0.2 705,119 - DDA3937_RS03230 0.61 +0.0 705,119 - DDA3937_RS03230 0.61 +0.8 705,197 - DDA3937_RS03230 0.71 -1.1 705,206 + DDA3937_RS03230 0.72 +0.8 705,206 + DDA3937_RS03230 0.72 +0.3 705,206 + DDA3937_RS03230 0.72 -0.1 705,206 + DDA3937_RS03230 0.72 -0.3 705,206 + DDA3937_RS03230 0.72 +0.8 705,206 + DDA3937_RS03230 0.72 +0.5 705,207 - DDA3937_RS03230 0.72 -0.2 705,207 - DDA3937_RS03230 0.72 +0.2 705,207 - DDA3937_RS03230 0.72 +0.5 705,207 - +2.3 705,207 - DDA3937_RS03230 0.72 +0.8 705,234 - DDA3937_RS03230 0.76 +2.0 705,277 + DDA3937_RS03230 0.81 +1.2 705,277 + DDA3937_RS03230 0.81 +0.1 705,277 + DDA3937_RS03230 0.81 +0.2 705,277 + DDA3937_RS03230 0.81 -1.1 705,277 + DDA3937_RS03230 0.81 -1.0 705,277 + DDA3937_RS03230 0.81 -1.8 705,277 + DDA3937_RS03230 0.81 -0.1 705,278 - DDA3937_RS03230 0.81 -3.2 705,278 - DDA3937_RS03230 0.81 +0.8 705,278 - DDA3937_RS03230 0.81 -0.2 705,278 - DDA3937_RS03230 0.81 -0.6 705,278 - DDA3937_RS03230 0.81 -1.8 705,278 - DDA3937_RS03230 0.81 +0.3 705,287 + DDA3937_RS03230 0.83 -0.2 705,306 + DDA3937_RS03230 0.85 -0.8 705,306 + DDA3937_RS03230 0.85 +3.8 705,306 + DDA3937_RS03230 0.85 +0.8 705,307 - DDA3937_RS03230 0.85 -0.2 705,307 - DDA3937_RS03230 0.85 -0.1 705,307 - DDA3937_RS03230 0.85 -0.1 705,307 - DDA3937_RS03230 0.85 +0.2 705,378 + -0.5 705,379 - -0.0 705,438 + -0.7 705,461 + +0.3 705,461 + +0.8 705,462 - -0.5 705,472 + -0.2 705,488 + +1.1 705,488 + +0.3 705,488 + -0.6 705,489 - -0.5 705,513 - -2.4 705,532 + -0.3 705,532 + -0.2 705,561 + -1.2 705,561 + -0.4 705,561 + +0.5 705,561 + -0.7 705,562 - -1.1 705,562 - +0.5 705,562 - +2.1 705,562 - +0.6 705,562 - -0.8 705,562 - -1.2 705,562 - +0.0 705,562 - -0.8 705,562 - +0.3 705,590 - +0.6 705,665 + DDA3937_RS03235 0.11 -0.8 705,665 + DDA3937_RS03235 0.11 +1.8 705,880 - DDA3937_RS03235 0.24 -1.4 705,880 - DDA3937_RS03235 0.24 +0.2 705,880 - DDA3937_RS03235 0.24 -1.8 705,989 + DDA3937_RS03235 0.30 -0.2 706,091 - DDA3937_RS03235 0.36 +1.3 706,199 + DDA3937_RS03235 0.43 -0.4 706,199 + DDA3937_RS03235 0.43 +0.1 706,199 + DDA3937_RS03235 0.43 -1.4 706,200 - DDA3937_RS03235 0.43 +0.3 706,203 + DDA3937_RS03235 0.43 -0.3 706,203 + DDA3937_RS03235 0.43 -0.3 706,203 + DDA3937_RS03235 0.43 -0.2 706,204 - DDA3937_RS03235 0.43 +0.4 706,294 + DDA3937_RS03235 0.48 +0.6 706,294 + DDA3937_RS03235 0.48 +0.0 706,294 + DDA3937_RS03235 0.48 +0.0 706,294 + DDA3937_RS03235 0.48 +0.4 706,295 - DDA3937_RS03235 0.49 -0.8 706,295 - DDA3937_RS03235 0.49 -1.4 706,295 - DDA3937_RS03235 0.49 +0.8 706,311 - DDA3937_RS03235 0.49 +0.7 706,341 + DDA3937_RS03235 0.51 +0.3 706,341 + DDA3937_RS03235 0.51 -1.2 706,385 + DDA3937_RS03235 0.54 +0.2 706,385 + DDA3937_RS03235 0.54 +0.3 706,385 + DDA3937_RS03235 0.54 -1.0 706,386 - DDA3937_RS03235 0.54 -0.2 706,634 + DDA3937_RS03235 0.69 +0.6 706,635 - DDA3937_RS03235 0.69 +2.3 706,643 + DDA3937_RS03235 0.69 -3.6 706,644 - DDA3937_RS03235 0.69 +0.4 706,644 - DDA3937_RS03235 0.69 -1.8 706,644 - DDA3937_RS03235 0.69 -0.0 706,695 + DDA3937_RS03235 0.72 +0.3 706,908 + DDA3937_RS03235 0.85 -0.8 706,909 - DDA3937_RS03235 0.85 -0.1 706,982 + DDA3937_RS03235 0.89 +0.0 706,982 + DDA3937_RS03235 0.89 +0.8 707,009 + -2.2 707,121 + +0.0 707,121 + +1.3 707,122 - -2.1 707,163 + -0.2 707,163 + -0.8 707,164 - -1.0 707,164 - -1.4 707,166 + -1.6 707,167 - -0.1 707,184 + -0.5 707,185 - -1.6 707,185 - -0.2 707,192 + +0.7 707,226 + +0.5 707,244 - +1.2 707,244 - -1.7 707,254 - -1.2 707,263 - +0.1 707,263 - +0.2 707,328 + +0.5 707,328 + +0.1 707,328 + -0.2 707,328 + -0.7 707,328 + +0.0 707,328 + -0.2 707,328 + +0.2 707,329 - -0.2 707,329 - -0.8 707,329 - -1.3 707,329 - -2.2 707,331 + -1.8 707,454 + -0.8 707,460 + +0.9 707,460 + -0.1 707,461 - -1.1 707,461 - -1.9 707,461 - -0.1 707,464 + +0.1 707,464 + -1.2 707,464 + +1.1 707,464 + +0.1 707,464 + +2.3 707,464 + -0.8 707,464 + -1.2 707,465 - +0.8 707,465 - +1.1 707,465 - -0.2 707,465 - -0.1 707,465 - +0.4 707,465 - +2.1 707,490 + -1.0 707,490 + -1.6 707,490 + -0.7 707,491 - +0.4 707,491 - -0.4 707,491 - +0.6 707,491 - +1.8 707,502 + -2.8 707,502 + -0.3 707,502 + -2.6 707,503 - +0.5 707,503 - +0.1 707,658 - DDA3937_RS03240 0.15 -0.2 707,714 + DDA3937_RS03240 0.20 +0.0 707,714 + +0.8 707,757 + DDA3937_RS03240 0.24 +0.8 707,757 + DDA3937_RS03240 0.24 -0.1 707,821 + DDA3937_RS03240 0.29 +0.8 707,822 - DDA3937_RS03240 0.29 -0.4 707,823 + DDA3937_RS03240 0.29 +0.5 707,823 + DDA3937_RS03240 0.29 +0.9 707,823 + DDA3937_RS03240 0.29 -1.2 707,823 + DDA3937_RS03240 0.29 -0.7 707,823 + DDA3937_RS03240 0.29 -1.4 707,824 - DDA3937_RS03240 0.29 +0.1 707,824 - DDA3937_RS03240 0.29 -0.3 707,824 - DDA3937_RS03240 0.29 +0.2 707,824 - DDA3937_RS03240 0.29 +0.3 707,824 - DDA3937_RS03240 0.29 -0.5 707,824 - DDA3937_RS03240 0.29 +0.7 707,824 - DDA3937_RS03240 0.29 -2.3 707,824 - DDA3937_RS03240 0.29 -0.4 707,896 + -1.2 707,896 + DDA3937_RS03240 0.36 -1.7 707,897 - DDA3937_RS03240 0.36 +0.8 707,897 - DDA3937_RS03240 0.36 -0.1 707,898 + DDA3937_RS03240 0.36 +0.2 707,898 + DDA3937_RS03240 0.36 -1.1 707,898 + DDA3937_RS03240 0.36 -2.2 707,898 + DDA3937_RS03240 0.36 -0.7 707,898 + DDA3937_RS03240 0.36 +0.3 707,898 + DDA3937_RS03240 0.36 -0.1 707,898 + DDA3937_RS03240 0.36 -2.1 707,899 - DDA3937_RS03240 0.36 -0.1 707,899 - DDA3937_RS03240 0.36 -0.6 707,899 - DDA3937_RS03240 0.36 -0.8 707,899 - DDA3937_RS03240 0.36 +1.1 707,899 - DDA3937_RS03240 0.36 +0.4 707,899 - DDA3937_RS03240 0.36 -1.8 708,003 - DDA3937_RS03240 0.45 -1.2 708,026 - DDA3937_RS03240 0.47 -0.9 708,131 - DDA3937_RS03240 0.56 -1.1
Or see this region's nucleotide sequence