Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10055 and DZA65_RS10060 are separated by 4 nucleotides DZA65_RS10060 and DZA65_RS10065 are separated by 161 nucleotides DZA65_RS10065 and DZA65_RS10070 are separated by 259 nucleotides
DZA65_RS10055: DZA65_RS10055 - histidinol dehydrogenase, at 2,128,086 to 2,129,396
_RS10055
DZA65_RS10060: DZA65_RS10060 - ATP phosphoribosyltransferase, at 2,129,401 to 2,130,300
_RS10060
DZA65_RS10065: DZA65_RS10065 - his operon leader peptide, at 2,130,462 to 2,130,509
_RS10065
DZA65_RS10070: DZA65_RS10070 - SDR family oxidoreductase, at 2,130,769 to 2,131,590
_RS10070
Position (kb)
2129
2130
2131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.754 kb on - strand, within DZA65_RS10055 at 2128.754 kb on - strand, within DZA65_RS10055 at 2129.000 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.003 kb on - strand, within DZA65_RS10055 at 2129.003 kb on - strand, within DZA65_RS10055 at 2129.402 kb on + strand at 2129.436 kb on + strand at 2129.436 kb on + strand at 2129.437 kb on - strand at 2129.618 kb on + strand, within DZA65_RS10060 at 2129.618 kb on + strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.736 kb on - strand, within DZA65_RS10060 at 2129.736 kb on - strand, within DZA65_RS10060 at 2129.802 kb on + strand, within DZA65_RS10060 at 2129.803 kb on - strand, within DZA65_RS10060 at 2129.889 kb on - strand, within DZA65_RS10060 at 2129.889 kb on - strand, within DZA65_RS10060 at 2129.928 kb on + strand, within DZA65_RS10060 at 2129.954 kb on + strand, within DZA65_RS10060 at 2129.955 kb on - strand, within DZA65_RS10060 at 2130.024 kb on - strand, within DZA65_RS10060 at 2130.024 kb on - strand, within DZA65_RS10060 at 2130.280 kb on + strand at 2130.280 kb on + strand at 2130.281 kb on - strand at 2130.291 kb on + strand at 2130.292 kb on - strand at 2130.314 kb on + strand at 2130.335 kb on + strand at 2130.449 kb on - strand at 2130.535 kb on - strand at 2130.535 kb on - strand at 2130.607 kb on - strand at 2130.615 kb on + strand at 2130.616 kb on - strand at 2130.616 kb on - strand at 2130.623 kb on - strand at 2130.631 kb on + strand at 2130.652 kb on + strand at 2130.652 kb on + strand at 2130.652 kb on + strand at 2130.652 kb on + strand at 2130.653 kb on - strand at 2130.654 kb on + strand at 2130.655 kb on - strand at 2130.655 kb on - strand at 2130.664 kb on - strand at 2130.664 kb on - strand at 2130.664 kb on - strand at 2130.668 kb on + strand at 2130.668 kb on + strand at 2130.668 kb on + strand at 2130.668 kb on + strand at 2130.669 kb on - strand at 2130.669 kb on - strand at 2130.669 kb on - strand at 2130.670 kb on + strand at 2130.670 kb on + strand at 2130.703 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.706 kb on - strand at 2130.706 kb on - strand at 2130.706 kb on - strand at 2130.706 kb on - strand at 2130.706 kb on - strand at 2130.706 kb on - strand at 2130.709 kb on + strand at 2130.709 kb on + strand at 2130.709 kb on + strand at 2130.709 kb on + strand at 2130.710 kb on - strand at 2130.710 kb on - strand at 2130.710 kb on - strand at 2130.710 kb on - strand at 2130.780 kb on - strand at 2131.030 kb on + strand, within DZA65_RS10070 at 2131.030 kb on + strand, within DZA65_RS10070 at 2131.030 kb on + strand, within DZA65_RS10070 at 2131.030 kb on + strand, within DZA65_RS10070 at 2131.031 kb on - strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.093 kb on + strand, within DZA65_RS10070 at 2131.093 kb on + strand, within DZA65_RS10070 at 2131.093 kb on + strand, within DZA65_RS10070 at 2131.093 kb on + strand, within DZA65_RS10070 at 2131.093 kb on + strand, within DZA65_RS10070 at 2131.094 kb on - strand, within DZA65_RS10070 at 2131.115 kb on - strand, within DZA65_RS10070 at 2131.154 kb on + strand, within DZA65_RS10070 at 2131.233 kb on + strand, within DZA65_RS10070 at 2131.233 kb on + strand, within DZA65_RS10070 at 2131.234 kb on - strand, within DZA65_RS10070
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,128,753 + DZA65_RS10055 0.51 -1.5 2,128,753 + +3.2 2,128,753 + DZA65_RS10055 0.51 +0.2 2,128,753 + DZA65_RS10055 0.51 -0.4 2,128,753 + DZA65_RS10055 0.51 -1.5 2,128,754 - DZA65_RS10055 0.51 -1.8 2,128,754 - DZA65_RS10055 0.51 +0.9 2,129,000 + DZA65_RS10055 0.70 +0.6 2,129,002 + DZA65_RS10055 0.70 -3.3 2,129,002 + DZA65_RS10055 0.70 -0.3 2,129,002 + DZA65_RS10055 0.70 -3.7 2,129,003 - DZA65_RS10055 0.70 -0.5 2,129,003 - DZA65_RS10055 0.70 -2.1 2,129,402 + -1.8 2,129,436 + -1.2 2,129,436 + -0.5 2,129,437 - -1.6 2,129,618 + DZA65_RS10060 0.24 +0.2 2,129,618 + DZA65_RS10060 0.24 -1.0 2,129,619 - DZA65_RS10060 0.24 -1.6 2,129,619 - DZA65_RS10060 0.24 -1.5 2,129,619 - DZA65_RS10060 0.24 -1.5 2,129,619 - DZA65_RS10060 0.24 -1.0 2,129,619 - DZA65_RS10060 0.24 -0.8 2,129,736 - DZA65_RS10060 0.37 -2.3 2,129,736 - DZA65_RS10060 0.37 -2.4 2,129,802 + DZA65_RS10060 0.45 -1.9 2,129,803 - DZA65_RS10060 0.45 -2.1 2,129,889 - DZA65_RS10060 0.54 -0.8 2,129,889 - DZA65_RS10060 0.54 -0.3 2,129,928 + DZA65_RS10060 0.59 -0.0 2,129,954 + DZA65_RS10060 0.61 -0.4 2,129,955 - DZA65_RS10060 0.62 -0.1 2,130,024 - DZA65_RS10060 0.69 -0.2 2,130,024 - DZA65_RS10060 0.69 -1.8 2,130,280 + -1.4 2,130,280 + +0.2 2,130,281 - -1.3 2,130,291 + -0.8 2,130,292 - +0.5 2,130,314 + -0.4 2,130,335 + -1.4 2,130,449 - -0.5 2,130,535 - -1.0 2,130,535 - +0.2 2,130,607 - -1.1 2,130,615 + -0.2 2,130,616 - -0.0 2,130,616 - +0.2 2,130,623 - -0.2 2,130,631 + +1.5 2,130,652 + -0.9 2,130,652 + -0.1 2,130,652 + +0.6 2,130,652 + -0.5 2,130,653 - -0.8 2,130,654 + +0.6 2,130,655 - -1.2 2,130,655 - -1.8 2,130,664 - -1.4 2,130,664 - +0.6 2,130,664 - -0.9 2,130,668 + +0.6 2,130,668 + +0.4 2,130,668 + +1.0 2,130,668 + +0.5 2,130,669 - -0.2 2,130,669 - +1.5 2,130,669 - +0.2 2,130,670 + -0.0 2,130,670 + -0.0 2,130,703 + +0.2 2,130,705 + -0.7 2,130,705 + -1.6 2,130,705 + -0.3 2,130,705 + -0.1 2,130,705 + +0.3 2,130,705 + +0.2 2,130,705 + -0.2 2,130,705 + +0.1 2,130,705 + -0.5 2,130,705 + +0.5 2,130,706 - +1.7 2,130,706 - +0.2 2,130,706 - +1.4 2,130,706 - +1.3 2,130,706 - +0.0 2,130,706 - +0.5 2,130,709 + -1.2 2,130,709 + -0.1 2,130,709 + -1.1 2,130,709 + +0.3 2,130,710 - +0.2 2,130,710 - -0.3 2,130,710 - -2.5 2,130,710 - -0.7 2,130,780 - +0.6 2,131,030 + DZA65_RS10070 0.32 -1.1 2,131,030 + DZA65_RS10070 0.32 +0.4 2,131,030 + DZA65_RS10070 0.32 -0.8 2,131,030 + DZA65_RS10070 0.32 -0.0 2,131,031 - DZA65_RS10070 0.32 +0.8 2,131,089 + DZA65_RS10070 0.39 -0.4 2,131,089 + DZA65_RS10070 0.39 +0.3 2,131,089 + DZA65_RS10070 0.39 -0.3 2,131,089 + DZA65_RS10070 0.39 -1.1 2,131,089 + DZA65_RS10070 0.39 -1.4 2,131,089 + DZA65_RS10070 0.39 -1.0 2,131,090 - DZA65_RS10070 0.39 -0.4 2,131,090 - DZA65_RS10070 0.39 +1.4 2,131,090 - DZA65_RS10070 0.39 -1.5 2,131,090 - DZA65_RS10070 0.39 +1.2 2,131,090 - DZA65_RS10070 0.39 -0.2 2,131,090 - DZA65_RS10070 0.39 +0.8 2,131,090 - DZA65_RS10070 0.39 +1.9 2,131,093 + DZA65_RS10070 0.39 -0.8 2,131,093 + DZA65_RS10070 0.39 +0.6 2,131,093 + DZA65_RS10070 0.39 -0.3 2,131,093 + DZA65_RS10070 0.39 -0.2 2,131,093 + DZA65_RS10070 0.39 -0.7 2,131,094 - DZA65_RS10070 0.40 -1.1 2,131,115 - DZA65_RS10070 0.42 -3.3 2,131,154 + DZA65_RS10070 0.47 +0.2 2,131,233 + DZA65_RS10070 0.56 -0.5 2,131,233 + DZA65_RS10070 0.56 +0.2 2,131,234 - DZA65_RS10070 0.57 +0.6
Or see this region's nucleotide sequence