Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10020 and DZA65_RS10025 are separated by 280 nucleotides DZA65_RS10025 and DZA65_RS10030 overlap by 7 nucleotides DZA65_RS10030 and DZA65_RS10035 overlap by 19 nucleotides DZA65_RS10035 and DZA65_RS10040 are separated by 6 nucleotides
DZA65_RS10020: DZA65_RS10020 - hypothetical protein, at 2,122,354 to 2,122,956
_RS10020
DZA65_RS10025: DZA65_RS10025 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 2,123,237 to 2,123,851
_RS10025
DZA65_RS10030: DZA65_RS10030 - imidazole glycerol phosphate synthase subunit HisF, at 2,123,845 to 2,124,621
_RS10030
DZA65_RS10035: DZA65_RS10035 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,124,603 to 2,125,340
_RS10035
DZA65_RS10040: DZA65_RS10040 - imidazole glycerol phosphate synthase subunit HisH, at 2,125,347 to 2,125,937
_RS10040
Position (kb)
2123
2124
2125 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2122.845 kb on + strand, within DZA65_RS10020 at 2122.845 kb on + strand, within DZA65_RS10020 at 2122.845 kb on + strand, within DZA65_RS10020 at 2122.845 kb on + strand, within DZA65_RS10020 at 2122.845 kb on + strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.846 kb on - strand, within DZA65_RS10020 at 2122.860 kb on + strand, within DZA65_RS10020 at 2122.951 kb on + strand at 2122.951 kb on + strand at 2122.951 kb on + strand at 2122.952 kb on - strand at 2122.952 kb on - strand at 2122.952 kb on - strand at 2122.967 kb on + strand at 2122.989 kb on + strand at 2123.019 kb on + strand at 2123.101 kb on - strand at 2123.101 kb on - strand at 2123.156 kb on + strand at 2123.157 kb on - strand at 2123.275 kb on + strand at 2123.319 kb on + strand, within DZA65_RS10025 at 2123.319 kb on + strand, within DZA65_RS10025 at 2123.320 kb on - strand, within DZA65_RS10025 at 2123.436 kb on + strand, within DZA65_RS10025 at 2123.437 kb on - strand, within DZA65_RS10025 at 2123.451 kb on + strand, within DZA65_RS10025 at 2123.451 kb on + strand at 2123.477 kb on - strand, within DZA65_RS10025 at 2123.620 kb on + strand at 2123.691 kb on + strand, within DZA65_RS10025 at 2123.727 kb on - strand, within DZA65_RS10025 at 2123.745 kb on + strand, within DZA65_RS10025 at 2123.745 kb on + strand, within DZA65_RS10025 at 2123.783 kb on + strand, within DZA65_RS10025 at 2123.783 kb on + strand, within DZA65_RS10025 at 2123.784 kb on - strand, within DZA65_RS10025 at 2123.784 kb on - strand at 2123.784 kb on - strand, within DZA65_RS10025 at 2123.878 kb on - strand at 2124.159 kb on + strand, within DZA65_RS10030 at 2124.188 kb on + strand, within DZA65_RS10030 at 2124.188 kb on + strand, within DZA65_RS10030 at 2124.256 kb on + strand, within DZA65_RS10030 at 2124.468 kb on + strand, within DZA65_RS10030 at 2124.473 kb on + strand, within DZA65_RS10030 at 2124.571 kb on + strand at 2124.571 kb on + strand at 2124.699 kb on + strand, within DZA65_RS10035 at 2124.794 kb on + strand, within DZA65_RS10035 at 2124.794 kb on + strand, within DZA65_RS10035 at 2124.870 kb on + strand, within DZA65_RS10035 at 2125.293 kb on + strand at 2125.348 kb on + strand at 2125.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,122,845 + DZA65_RS10020 0.81 +0.9 2,122,845 + DZA65_RS10020 0.81 +2.0 2,122,845 + DZA65_RS10020 0.81 +0.7 2,122,845 + DZA65_RS10020 0.81 -1.4 2,122,845 + DZA65_RS10020 0.81 +0.7 2,122,846 - DZA65_RS10020 0.82 +0.1 2,122,846 - DZA65_RS10020 0.82 -0.7 2,122,846 - DZA65_RS10020 0.82 -1.8 2,122,846 - DZA65_RS10020 0.82 +1.1 2,122,846 - DZA65_RS10020 0.82 +0.5 2,122,846 - DZA65_RS10020 0.82 +1.0 2,122,846 - DZA65_RS10020 0.82 -1.4 2,122,846 - DZA65_RS10020 0.82 +0.3 2,122,846 - DZA65_RS10020 0.82 +0.2 2,122,846 - DZA65_RS10020 0.82 +0.2 2,122,860 + DZA65_RS10020 0.84 +0.8 2,122,951 + -1.4 2,122,951 + +0.2 2,122,951 + -0.2 2,122,952 - -0.8 2,122,952 - -0.2 2,122,952 - +3.2 2,122,967 + +0.4 2,122,989 + +0.5 2,123,019 + +0.2 2,123,101 - +0.2 2,123,101 - -1.0 2,123,156 + -1.1 2,123,157 - +1.0 2,123,275 + -1.4 2,123,319 + DZA65_RS10025 0.13 -0.8 2,123,319 + DZA65_RS10025 0.13 +0.5 2,123,320 - DZA65_RS10025 0.13 -2.1 2,123,436 + DZA65_RS10025 0.32 -1.1 2,123,437 - DZA65_RS10025 0.33 -0.2 2,123,451 + DZA65_RS10025 0.35 +0.1 2,123,451 + -0.4 2,123,477 - DZA65_RS10025 0.39 -1.4 2,123,620 + -0.8 2,123,691 + DZA65_RS10025 0.74 +0.7 2,123,727 - DZA65_RS10025 0.80 -0.4 2,123,745 + DZA65_RS10025 0.83 -0.5 2,123,745 + DZA65_RS10025 0.83 -3.4 2,123,783 + DZA65_RS10025 0.89 -3.0 2,123,783 + DZA65_RS10025 0.89 -2.0 2,123,784 - DZA65_RS10025 0.89 -1.6 2,123,784 - -0.4 2,123,784 - DZA65_RS10025 0.89 -2.1 2,123,878 - -0.3 2,124,159 + DZA65_RS10030 0.40 -2.4 2,124,188 + DZA65_RS10030 0.44 +1.9 2,124,188 + DZA65_RS10030 0.44 -1.6 2,124,256 + DZA65_RS10030 0.53 -1.8 2,124,468 + DZA65_RS10030 0.80 -1.6 2,124,473 + DZA65_RS10030 0.81 -1.7 2,124,571 + -1.4 2,124,571 + -1.0 2,124,699 + DZA65_RS10035 0.13 -0.4 2,124,794 + DZA65_RS10035 0.26 -0.1 2,124,794 + DZA65_RS10035 0.26 -0.4 2,124,870 + DZA65_RS10035 0.36 -2.6 2,125,293 + -0.8 2,125,348 + -1.6 2,125,371 + -0.2
Or see this region's nucleotide sequence