Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS08135 and DZA65_RS08140 are separated by 74 nucleotides DZA65_RS08140 and DZA65_RS08145 are separated by 3 nucleotides DZA65_RS08145 and DZA65_RS08150 are separated by 89 nucleotides DZA65_RS08150 and DZA65_RS08155 overlap by 4 nucleotides
DZA65_RS08135: DZA65_RS08135 - RraA family protein, at 1,703,415 to 1,704,032
_RS08135
DZA65_RS08140: DZA65_RS08140 - type II toxin-antitoxin system HicB family antitoxin, at 1,704,107 to 1,704,517
_RS08140
DZA65_RS08145: DZA65_RS08145 - type II toxin-antitoxin system HicA family toxin, at 1,704,521 to 1,704,673
_RS08145
DZA65_RS08150: DZA65_RS08150 - hypothetical protein, at 1,704,763 to 1,705,641
_RS08150
DZA65_RS08155: DZA65_RS08155 - helix-turn-helix transcriptional regulator, at 1,705,638 to 1,706,006
_RS08155
Position (kb)
1704
1705 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1703.521 kb on - strand, within DZA65_RS08135 at 1703.608 kb on + strand, within DZA65_RS08135 at 1703.608 kb on + strand, within DZA65_RS08135 at 1703.927 kb on + strand, within DZA65_RS08135 at 1704.030 kb on + strand at 1704.030 kb on + strand at 1704.030 kb on + strand at 1704.030 kb on + strand at 1704.030 kb on + strand at 1704.030 kb on + strand at 1704.031 kb on - strand at 1704.031 kb on - strand at 1704.101 kb on + strand at 1704.102 kb on - strand at 1704.167 kb on - strand, within DZA65_RS08140 at 1704.232 kb on + strand, within DZA65_RS08140 at 1704.291 kb on + strand, within DZA65_RS08140 at 1704.300 kb on + strand, within DZA65_RS08140 at 1704.347 kb on - strand, within DZA65_RS08140 at 1704.347 kb on - strand, within DZA65_RS08140 at 1704.471 kb on + strand, within DZA65_RS08140 at 1704.472 kb on - strand, within DZA65_RS08140 at 1704.482 kb on - strand at 1704.511 kb on - strand at 1704.517 kb on + strand at 1704.517 kb on + strand at 1704.518 kb on - strand at 1704.518 kb on - strand at 1704.585 kb on - strand, within DZA65_RS08145 at 1704.591 kb on - strand, within DZA65_RS08145 at 1704.602 kb on - strand, within DZA65_RS08145 at 1704.620 kb on + strand, within DZA65_RS08145 at 1704.644 kb on - strand, within DZA65_RS08145 at 1704.644 kb on - strand, within DZA65_RS08145 at 1704.696 kb on + strand at 1704.697 kb on - strand at 1704.793 kb on - strand at 1704.862 kb on + strand, within DZA65_RS08150 at 1704.901 kb on - strand, within DZA65_RS08150 at 1704.920 kb on + strand, within DZA65_RS08150 at 1704.920 kb on + strand, within DZA65_RS08150 at 1704.921 kb on - strand, within DZA65_RS08150 at 1704.940 kb on + strand, within DZA65_RS08150 at 1704.999 kb on + strand, within DZA65_RS08150 at 1705.004 kb on - strand, within DZA65_RS08150 at 1705.017 kb on + strand, within DZA65_RS08150 at 1705.021 kb on - strand, within DZA65_RS08150 at 1705.021 kb on - strand, within DZA65_RS08150 at 1705.034 kb on - strand, within DZA65_RS08150 at 1705.051 kb on - strand, within DZA65_RS08150 at 1705.098 kb on + strand, within DZA65_RS08150 at 1705.099 kb on - strand, within DZA65_RS08150 at 1705.099 kb on - strand, within DZA65_RS08150 at 1705.122 kb on + strand, within DZA65_RS08150 at 1705.123 kb on - strand, within DZA65_RS08150 at 1705.129 kb on + strand, within DZA65_RS08150 at 1705.129 kb on + strand, within DZA65_RS08150 at 1705.135 kb on + strand, within DZA65_RS08150 at 1705.135 kb on + strand, within DZA65_RS08150 at 1705.136 kb on - strand, within DZA65_RS08150 at 1705.178 kb on + strand, within DZA65_RS08150 at 1705.207 kb on + strand, within DZA65_RS08150 at 1705.228 kb on + strand, within DZA65_RS08150 at 1705.330 kb on + strand, within DZA65_RS08150 at 1705.343 kb on + strand, within DZA65_RS08150 at 1705.348 kb on + strand, within DZA65_RS08150 at 1705.348 kb on + strand, within DZA65_RS08150 at 1705.423 kb on - strand, within DZA65_RS08150 at 1705.424 kb on + strand, within DZA65_RS08150 at 1705.424 kb on + strand, within DZA65_RS08150 at 1705.425 kb on - strand, within DZA65_RS08150 at 1705.448 kb on - strand, within DZA65_RS08150 at 1705.448 kb on - strand, within DZA65_RS08150 at 1705.450 kb on - strand, within DZA65_RS08150 at 1705.477 kb on - strand, within DZA65_RS08150 at 1705.485 kb on - strand, within DZA65_RS08150 at 1705.488 kb on + strand, within DZA65_RS08150 at 1705.488 kb on + strand, within DZA65_RS08150 at 1705.527 kb on - strand, within DZA65_RS08150 at 1705.554 kb on - strand at 1705.604 kb on - strand at 1705.604 kb on - strand at 1705.635 kb on - strand at 1705.635 kb on - strand at 1705.635 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,703,521 - DZA65_RS08135 0.17 -0.1 1,703,608 + DZA65_RS08135 0.31 -0.9 1,703,608 + DZA65_RS08135 0.31 +0.1 1,703,927 + DZA65_RS08135 0.83 -0.2 1,704,030 + -0.7 1,704,030 + +0.4 1,704,030 + -0.2 1,704,030 + +1.1 1,704,030 + -0.7 1,704,030 + +0.4 1,704,031 - -0.3 1,704,031 - -1.4 1,704,101 + -0.6 1,704,102 - -1.7 1,704,167 - DZA65_RS08140 0.15 +0.3 1,704,232 + DZA65_RS08140 0.30 -0.7 1,704,291 + DZA65_RS08140 0.45 +0.6 1,704,300 + DZA65_RS08140 0.47 -0.3 1,704,347 - DZA65_RS08140 0.58 -0.7 1,704,347 - DZA65_RS08140 0.58 +1.9 1,704,471 + DZA65_RS08140 0.89 -2.0 1,704,472 - DZA65_RS08140 0.89 -0.1 1,704,482 - +1.1 1,704,511 - +0.6 1,704,517 + +0.0 1,704,517 + +0.3 1,704,518 - +0.3 1,704,518 - -0.7 1,704,585 - DZA65_RS08145 0.42 +0.8 1,704,591 - DZA65_RS08145 0.46 -0.0 1,704,602 - DZA65_RS08145 0.53 -0.2 1,704,620 + DZA65_RS08145 0.65 +0.3 1,704,644 - DZA65_RS08145 0.80 -1.3 1,704,644 - DZA65_RS08145 0.80 +1.4 1,704,696 + +0.8 1,704,697 - -0.4 1,704,793 - +0.8 1,704,862 + DZA65_RS08150 0.11 -0.5 1,704,901 - DZA65_RS08150 0.16 -1.5 1,704,920 + DZA65_RS08150 0.18 -0.4 1,704,920 + DZA65_RS08150 0.18 +0.2 1,704,921 - DZA65_RS08150 0.18 -0.3 1,704,940 + DZA65_RS08150 0.20 +0.2 1,704,999 + DZA65_RS08150 0.27 +0.3 1,705,004 - DZA65_RS08150 0.27 -0.1 1,705,017 + DZA65_RS08150 0.29 -0.0 1,705,021 - DZA65_RS08150 0.29 -1.1 1,705,021 - DZA65_RS08150 0.29 +0.5 1,705,034 - DZA65_RS08150 0.31 -0.3 1,705,051 - DZA65_RS08150 0.33 +0.0 1,705,098 + DZA65_RS08150 0.38 +1.3 1,705,099 - DZA65_RS08150 0.38 -0.5 1,705,099 - DZA65_RS08150 0.38 +0.6 1,705,122 + DZA65_RS08150 0.41 +0.4 1,705,123 - DZA65_RS08150 0.41 +1.1 1,705,129 + DZA65_RS08150 0.42 -0.9 1,705,129 + DZA65_RS08150 0.42 -0.1 1,705,135 + DZA65_RS08150 0.42 +0.3 1,705,135 + DZA65_RS08150 0.42 +0.3 1,705,136 - DZA65_RS08150 0.42 -0.0 1,705,178 + DZA65_RS08150 0.47 +1.5 1,705,207 + DZA65_RS08150 0.51 -1.0 1,705,228 + DZA65_RS08150 0.53 -0.6 1,705,330 + DZA65_RS08150 0.65 +0.4 1,705,343 + DZA65_RS08150 0.66 -0.2 1,705,348 + DZA65_RS08150 0.67 -0.7 1,705,348 + DZA65_RS08150 0.67 -0.1 1,705,423 - DZA65_RS08150 0.75 -1.1 1,705,424 + DZA65_RS08150 0.75 -0.2 1,705,424 + DZA65_RS08150 0.75 +0.3 1,705,425 - DZA65_RS08150 0.75 -1.1 1,705,448 - DZA65_RS08150 0.78 -0.2 1,705,448 - DZA65_RS08150 0.78 -0.3 1,705,450 - DZA65_RS08150 0.78 -0.3 1,705,477 - DZA65_RS08150 0.81 -0.9 1,705,485 - DZA65_RS08150 0.82 +0.5 1,705,488 + DZA65_RS08150 0.82 +1.1 1,705,488 + DZA65_RS08150 0.82 +1.2 1,705,527 - DZA65_RS08150 0.87 -0.7 1,705,554 - -0.3 1,705,604 - +0.3 1,705,604 - +0.3 1,705,635 - -0.8 1,705,635 - +0.3 1,705,635 - +0.3
Or see this region's nucleotide sequence