Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS03175 and DZA65_RS03180 are separated by 264 nucleotides DZA65_RS03180 and DZA65_RS03185 are separated by 123 nucleotides DZA65_RS03185 and DZA65_RS03190 are separated by 157 nucleotides DZA65_RS03190 and DZA65_RS03195 are separated by 47 nucleotides
DZA65_RS03175: DZA65_RS03175 - serine-type D-Ala-D-Ala carboxypeptidase, at 674,044 to 675,477
_RS03175
DZA65_RS03180: DZA65_RS03180 - transcription elongation factor GreA, at 675,742 to 676,218
_RS03180
DZA65_RS03185: DZA65_RS03185 - ribosome assembly RNA-binding protein YhbY, at 676,342 to 676,635
_RS03185
DZA65_RS03190: DZA65_RS03190 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 676,793 to 677,422
_RS03190
DZA65_RS03195: DZA65_RS03195 - ATP-dependent zinc metalloprotease FtsH, at 677,470 to 679,422
_RS03195
Position (kb)
676
677 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 675.392 kb on + strand at 675.393 kb on - strand at 675.401 kb on + strand at 675.401 kb on + strand at 675.402 kb on - strand at 675.402 kb on - strand at 675.402 kb on - strand at 675.478 kb on - strand at 675.478 kb on - strand at 675.492 kb on - strand at 675.492 kb on - strand at 675.512 kb on + strand at 675.512 kb on + strand at 675.513 kb on - strand at 675.513 kb on - strand at 675.513 kb on - strand at 675.513 kb on - strand at 675.515 kb on + strand at 675.516 kb on - strand at 675.537 kb on + strand at 675.538 kb on - strand at 675.626 kb on + strand at 675.626 kb on + strand at 675.627 kb on - strand at 675.636 kb on + strand at 675.684 kb on + strand at 675.684 kb on + strand at 675.684 kb on + strand at 675.684 kb on + strand at 675.684 kb on + strand at 675.684 kb on + strand at 675.685 kb on - strand at 675.685 kb on - strand at 675.685 kb on - strand at 675.685 kb on - strand at 675.685 kb on - strand at 675.727 kb on - strand at 675.732 kb on + strand at 675.732 kb on + strand at 675.732 kb on + strand at 675.732 kb on + strand at 675.732 kb on + strand at 675.742 kb on - strand at 675.783 kb on - strand at 675.828 kb on + strand, within DZA65_RS03180 at 675.828 kb on + strand, within DZA65_RS03180 at 675.883 kb on + strand, within DZA65_RS03180 at 675.883 kb on + strand, within DZA65_RS03180 at 675.883 kb on + strand, within DZA65_RS03180 at 675.884 kb on - strand, within DZA65_RS03180 at 675.884 kb on - strand, within DZA65_RS03180 at 675.964 kb on - strand, within DZA65_RS03180 at 676.012 kb on - strand, within DZA65_RS03180 at 676.054 kb on + strand, within DZA65_RS03180 at 676.054 kb on + strand, within DZA65_RS03180 at 676.055 kb on - strand, within DZA65_RS03180 at 676.055 kb on - strand, within DZA65_RS03180 at 676.059 kb on + strand, within DZA65_RS03180 at 676.098 kb on + strand, within DZA65_RS03180 at 676.098 kb on + strand, within DZA65_RS03180 at 676.098 kb on + strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.099 kb on - strand, within DZA65_RS03180 at 676.133 kb on + strand, within DZA65_RS03180 at 676.184 kb on + strand at 676.184 kb on + strand at 676.185 kb on - strand at 676.185 kb on - strand at 676.210 kb on + strand at 676.211 kb on - strand at 676.211 kb on - strand at 676.251 kb on + strand at 676.251 kb on + strand at 676.279 kb on + strand at 676.383 kb on - strand, within DZA65_RS03185 at 676.535 kb on - strand, within DZA65_RS03185 at 676.535 kb on - strand, within DZA65_RS03185 at 676.535 kb on - strand, within DZA65_RS03185 at 676.623 kb on + strand at 676.676 kb on + strand at 676.693 kb on + strand at 676.693 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.764 kb on + strand at 676.764 kb on + strand at 676.779 kb on + strand at 677.420 kb on + strand at 677.420 kb on + strand at 677.420 kb on + strand at 677.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 675,392 + +2.0 675,393 - +1.8 675,401 + +0.4 675,401 + +1.2 675,402 - +1.1 675,402 - -0.6 675,402 - +0.9 675,478 - -0.7 675,478 - -0.0 675,492 - -0.1 675,492 - -0.5 675,512 + -0.1 675,512 + +1.0 675,513 - +0.0 675,513 - -1.9 675,513 - -2.1 675,513 - +1.5 675,515 + -1.1 675,516 - +2.5 675,537 + +0.3 675,538 - +0.3 675,626 + -1.6 675,626 + -0.9 675,627 - -1.5 675,636 + -1.5 675,684 + +0.0 675,684 + -1.1 675,684 + -1.1 675,684 + -0.5 675,684 + -0.5 675,684 + +0.2 675,685 - -0.3 675,685 - -0.9 675,685 - +0.7 675,685 - +1.9 675,685 - -0.9 675,727 - +0.5 675,732 + -0.2 675,732 + +0.2 675,732 + +0.0 675,732 + -0.2 675,732 + -0.1 675,742 - -0.3 675,783 - -0.4 675,828 + DZA65_RS03180 0.18 -0.3 675,828 + DZA65_RS03180 0.18 -0.1 675,883 + DZA65_RS03180 0.30 -0.5 675,883 + DZA65_RS03180 0.30 -0.5 675,883 + DZA65_RS03180 0.30 -0.1 675,884 - DZA65_RS03180 0.30 -0.4 675,884 - DZA65_RS03180 0.30 -0.9 675,964 - DZA65_RS03180 0.47 -0.6 676,012 - DZA65_RS03180 0.57 -0.8 676,054 + DZA65_RS03180 0.65 -0.3 676,054 + DZA65_RS03180 0.65 -0.8 676,055 - DZA65_RS03180 0.66 +0.5 676,055 - DZA65_RS03180 0.66 +0.5 676,059 + DZA65_RS03180 0.66 +0.5 676,098 + DZA65_RS03180 0.75 -0.2 676,098 + DZA65_RS03180 0.75 +0.2 676,098 + DZA65_RS03180 0.75 +2.0 676,099 - DZA65_RS03180 0.75 +0.0 676,099 - DZA65_RS03180 0.75 -0.8 676,099 - DZA65_RS03180 0.75 +0.5 676,099 - DZA65_RS03180 0.75 +0.9 676,099 - DZA65_RS03180 0.75 +0.2 676,099 - DZA65_RS03180 0.75 +0.8 676,133 + DZA65_RS03180 0.82 -0.4 676,184 + -0.1 676,184 + +0.1 676,185 - -1.2 676,185 - -0.0 676,210 + -0.8 676,211 - +0.5 676,211 - -0.4 676,251 + -2.0 676,251 + -1.1 676,279 + +0.5 676,383 - DZA65_RS03185 0.14 -2.0 676,535 - DZA65_RS03185 0.66 -0.7 676,535 - DZA65_RS03185 0.66 -0.3 676,535 - DZA65_RS03185 0.66 -1.9 676,623 + -1.1 676,676 + -0.2 676,693 + +0.4 676,693 + +0.5 676,754 + +0.9 676,754 + +0.2 676,754 + +0.3 676,754 + -0.9 676,754 + -0.3 676,754 + -0.3 676,758 + -1.1 676,758 + -0.5 676,758 + -0.0 676,758 + -1.9 676,764 + +0.6 676,764 + +0.4 676,779 + +0.5 677,420 + -0.8 677,420 + -0.3 677,420 + -0.6 677,423 + -0.3
Or see this region's nucleotide sequence