Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS02050 and DZA65_RS02055 are separated by 13 nucleotides DZA65_RS02055 and DZA65_RS02060 are separated by 114 nucleotides DZA65_RS02060 and DZA65_RS02065 are separated by 283 nucleotides
DZA65_RS02050: DZA65_RS02050 - valine--tRNA ligase, at 450,869 to 453,739
_RS02050
DZA65_RS02055: DZA65_RS02055 - DNA polymerase III subunit chi, at 453,753 to 454,238
_RS02055
DZA65_RS02060: DZA65_RS02060 - leucyl aminopeptidase, at 454,353 to 455,861
_RS02060
DZA65_RS02065: DZA65_RS02065 - LPS export ABC transporter permease LptF, at 456,145 to 457,263
_RS02065
Position (kb) 454
455
456 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 454.275 kb on - strand at 454.354 kb on + strand at 454.484 kb on - strand at 454.588 kb on + strand, within DZA65_RS02060 at 454.772 kb on - strand, within DZA65_RS02060 at 454.772 kb on - strand, within DZA65_RS02060 at 454.772 kb on - strand, within DZA65_RS02060 at 454.990 kb on + strand, within DZA65_RS02060 at 455.104 kb on + strand, within DZA65_RS02060 at 455.448 kb on - strand, within DZA65_RS02060 at 455.449 kb on + strand, within DZA65_RS02060 at 455.450 kb on - strand, within DZA65_RS02060 at 455.511 kb on + strand, within DZA65_RS02060 at 455.511 kb on + strand at 455.512 kb on - strand, within DZA65_RS02060 at 455.512 kb on - strand, within DZA65_RS02060 at 455.512 kb on - strand, within DZA65_RS02060 at 455.512 kb on - strand, within DZA65_RS02060 at 455.667 kb on + strand, within DZA65_RS02060 at 455.668 kb on - strand, within DZA65_RS02060 at 455.864 kb on - strand at 455.899 kb on - strand at 455.905 kb on - strand at 455.949 kb on - strand at 455.958 kb on + strand at 455.959 kb on - strand at 455.959 kb on - strand at 455.959 kb on - strand at 455.964 kb on - strand at 455.992 kb on + strand at 456.009 kb on + strand at 456.035 kb on + strand at 456.035 kb on + strand at 456.050 kb on + strand at 456.074 kb on + strand at 456.074 kb on + strand at 456.074 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 454,275 - +0.5 454,354 + -2.9 454,484 - -0.1 454,588 + DZA65_RS02060 0.16 -0.2 454,772 - DZA65_RS02060 0.28 -1.8 454,772 - DZA65_RS02060 0.28 +0.0 454,772 - DZA65_RS02060 0.28 -0.6 454,990 + DZA65_RS02060 0.42 -0.8 455,104 + DZA65_RS02060 0.50 -0.9 455,448 - DZA65_RS02060 0.73 -1.3 455,449 + DZA65_RS02060 0.73 -1.5 455,450 - DZA65_RS02060 0.73 -1.9 455,511 + DZA65_RS02060 0.77 -0.0 455,511 + -1.1 455,512 - DZA65_RS02060 0.77 -1.9 455,512 - DZA65_RS02060 0.77 -0.5 455,512 - DZA65_RS02060 0.77 -1.7 455,512 - DZA65_RS02060 0.77 -0.8 455,667 + DZA65_RS02060 0.87 +0.5 455,668 - DZA65_RS02060 0.87 -1.1 455,864 - -2.4 455,899 - +0.6 455,905 - +1.5 455,949 - -1.2 455,958 + -0.9 455,959 - +0.8 455,959 - +0.9 455,959 - -0.3 455,964 - +0.7 455,992 + +0.2 456,009 + +0.7 456,035 + -0.9 456,035 + -0.7 456,050 + +0.3 456,074 + -0.2 456,074 + +0.4 456,074 + -0.0
Or see this region's nucleotide sequence