Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01165 and DZA65_RS01170 are separated by 243 nucleotides DZA65_RS01170 and DZA65_RS01175 are separated by 275 nucleotides DZA65_RS01175 and DZA65_RS01180 are separated by 259 nucleotides DZA65_RS01180 and DZA65_RS22770 are separated by 24 nucleotides
DZA65_RS01165: DZA65_RS01165 - DNA-directed RNA polymerase subunit beta', at 259,395 to 263,618
_RS01165
DZA65_RS01170: DZA65_RS01170 - DUF1176 domain-containing protein, at 263,862 to 264,959
_RS01170
DZA65_RS01175: DZA65_RS01175 - DUF1127 domain-containing protein, at 265,235 to 265,414
_RS01175
DZA65_RS01180: DZA65_RS01180 - DUF1127 domain-containing protein, at 265,674 to 265,865
_RS01180
DZA65_RS22770: DZA65_RS22770 - hypothetical protein, at 265,890 to 266,033
_RS22770
Position (kb) 263
264
265 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 263.649 kb on - strand at 263.677 kb on - strand at 263.679 kb on - strand at 263.684 kb on + strand at 263.684 kb on + strand at 263.684 kb on + strand at 263.684 kb on + strand at 263.684 kb on + strand at 263.684 kb on + strand at 263.684 kb on + strand at 263.684 kb on + strand at 263.684 kb on + strand at 263.684 kb on + strand at 263.685 kb on - strand at 263.685 kb on - strand at 263.685 kb on - strand at 263.704 kb on + strand at 263.704 kb on + strand at 263.708 kb on + strand at 263.709 kb on - strand at 263.773 kb on + strand at 263.816 kb on + strand at 263.816 kb on + strand at 263.816 kb on + strand at 263.816 kb on + strand at 263.816 kb on + strand at 263.816 kb on + strand at 263.816 kb on + strand at 263.816 kb on + strand at 263.816 kb on + strand at 263.816 kb on + strand at 263.817 kb on - strand at 263.817 kb on - strand at 263.817 kb on - strand at 263.817 kb on - strand at 263.817 kb on - strand at 263.817 kb on - strand at 263.826 kb on - strand at 263.827 kb on + strand at 263.827 kb on + strand at 263.827 kb on + strand at 263.827 kb on + strand at 263.828 kb on - strand at 263.828 kb on - strand at 263.828 kb on - strand at 263.849 kb on + strand at 263.850 kb on - strand at 263.850 kb on - strand at 263.873 kb on + strand at 263.874 kb on - strand at 263.968 kb on + strand at 263.968 kb on + strand at 263.968 kb on + strand at 263.969 kb on - strand at 263.969 kb on - strand at 263.987 kb on + strand, within DZA65_RS01170 at 263.987 kb on + strand, within DZA65_RS01170 at 263.988 kb on - strand, within DZA65_RS01170 at 263.988 kb on - strand, within DZA65_RS01170 at 263.988 kb on - strand, within DZA65_RS01170 at 263.988 kb on - strand, within DZA65_RS01170 at 263.988 kb on - strand, within DZA65_RS01170 at 263.988 kb on - strand, within DZA65_RS01170 at 263.993 kb on + strand, within DZA65_RS01170 at 263.993 kb on + strand, within DZA65_RS01170 at 263.993 kb on + strand, within DZA65_RS01170 at 263.993 kb on + strand, within DZA65_RS01170 at 263.993 kb on + strand, within DZA65_RS01170 at 263.994 kb on - strand, within DZA65_RS01170 at 263.994 kb on - strand, within DZA65_RS01170 at 263.994 kb on - strand, within DZA65_RS01170 at 264.050 kb on - strand, within DZA65_RS01170 at 264.050 kb on - strand, within DZA65_RS01170 at 264.120 kb on + strand, within DZA65_RS01170 at 264.120 kb on + strand, within DZA65_RS01170 at 264.121 kb on - strand, within DZA65_RS01170 at 264.189 kb on + strand, within DZA65_RS01170 at 264.189 kb on + strand, within DZA65_RS01170 at 264.189 kb on + strand, within DZA65_RS01170 at 264.190 kb on - strand, within DZA65_RS01170 at 264.190 kb on - strand, within DZA65_RS01170 at 264.190 kb on - strand, within DZA65_RS01170 at 264.190 kb on - strand, within DZA65_RS01170 at 264.198 kb on - strand, within DZA65_RS01170 at 264.287 kb on + strand, within DZA65_RS01170 at 264.288 kb on - strand, within DZA65_RS01170 at 264.290 kb on + strand, within DZA65_RS01170 at 264.291 kb on - strand, within DZA65_RS01170 at 264.291 kb on - strand, within DZA65_RS01170 at 264.453 kb on + strand, within DZA65_RS01170 at 264.453 kb on + strand, within DZA65_RS01170 at 264.453 kb on + strand, within DZA65_RS01170 at 264.454 kb on - strand, within DZA65_RS01170 at 264.454 kb on - strand, within DZA65_RS01170 at 264.454 kb on - strand, within DZA65_RS01170 at 264.454 kb on - strand, within DZA65_RS01170 at 264.456 kb on - strand, within DZA65_RS01170 at 264.753 kb on - strand, within DZA65_RS01170 at 264.766 kb on - strand, within DZA65_RS01170 at 264.766 kb on - strand, within DZA65_RS01170 at 264.766 kb on - strand, within DZA65_RS01170 at 264.789 kb on + strand, within DZA65_RS01170 at 264.789 kb on + strand, within DZA65_RS01170 at 264.789 kb on + strand, within DZA65_RS01170 at 264.789 kb on + strand, within DZA65_RS01170 at 264.789 kb on + strand, within DZA65_RS01170 at 264.790 kb on - strand, within DZA65_RS01170 at 264.790 kb on - strand, within DZA65_RS01170 at 264.790 kb on - strand, within DZA65_RS01170 at 264.790 kb on - strand, within DZA65_RS01170 at 264.854 kb on + strand at 264.855 kb on - strand at 264.876 kb on + strand at 264.876 kb on + strand at 264.876 kb on + strand at 264.877 kb on - strand at 264.877 kb on - strand at 264.877 kb on - strand at 264.882 kb on + strand at 264.883 kb on - strand at 264.883 kb on - strand at 264.883 kb on - strand at 264.883 kb on - strand at 264.896 kb on + strand at 264.896 kb on + strand at 264.896 kb on + strand at 264.897 kb on - strand at 264.897 kb on - strand at 265.039 kb on - strand at 265.039 kb on - strand at 265.057 kb on + strand at 265.074 kb on + strand at 265.074 kb on + strand at 265.075 kb on - strand at 265.075 kb on - strand at 265.075 kb on - strand at 265.075 kb on - strand at 265.143 kb on - strand at 265.143 kb on - strand at 265.143 kb on - strand at 265.151 kb on + strand at 265.151 kb on + strand at 265.152 kb on - strand at 265.152 kb on - strand at 265.154 kb on + strand at 265.208 kb on + strand at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.297 kb on + strand, within DZA65_RS01175 at 265.298 kb on - strand, within DZA65_RS01175 at 265.298 kb on - strand, within DZA65_RS01175 at 265.301 kb on + strand, within DZA65_RS01175 at 265.301 kb on + strand, within DZA65_RS01175 at 265.301 kb on + strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.302 kb on - strand, within DZA65_RS01175 at 265.344 kb on + strand, within DZA65_RS01175 at 265.344 kb on + strand, within DZA65_RS01175 at 265.401 kb on + strand at 265.401 kb on + strand at 265.421 kb on - strand at 265.439 kb on + strand at 265.440 kb on - strand at 265.440 kb on - strand at 265.442 kb on + strand at 265.442 kb on + strand at 265.442 kb on + strand at 265.442 kb on + strand at 265.443 kb on - strand at 265.443 kb on - strand at 265.443 kb on - strand at 265.443 kb on - strand at 265.472 kb on + strand at 265.472 kb on + strand at 265.502 kb on + strand at 265.503 kb on - strand at 265.518 kb on + strand at 265.519 kb on - strand at 265.632 kb on - strand at 265.650 kb on + strand at 265.650 kb on + strand at 265.651 kb on - strand at 265.651 kb on - strand at 265.718 kb on + strand, within DZA65_RS01180 at 265.745 kb on + strand, within DZA65_RS01180 at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.854 kb on + strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.855 kb on - strand at 265.857 kb on + strand at 265.857 kb on + strand at 265.857 kb on + strand at 265.857 kb on + strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.858 kb on - strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.893 kb on + strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.894 kb on - strand at 265.916 kb on + strand, within DZA65_RS22770
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 263,649 - -0.4 263,677 - -0.1 263,679 - -2.2 263,684 + +1.9 263,684 + -0.1 263,684 + -0.1 263,684 + +0.2 263,684 + -0.5 263,684 + -0.3 263,684 + -1.3 263,684 + +0.9 263,684 + -0.4 263,684 + +0.1 263,685 - +0.5 263,685 - -0.4 263,685 - +0.6 263,704 + +2.3 263,704 + -0.3 263,708 + -0.2 263,709 - +0.4 263,773 + +1.3 263,816 + -1.2 263,816 + +0.2 263,816 + +1.0 263,816 + -0.3 263,816 + +0.2 263,816 + -1.4 263,816 + -0.8 263,816 + -1.1 263,816 + +0.4 263,816 + +0.3 263,817 - -1.0 263,817 - -2.2 263,817 - -0.0 263,817 - -0.8 263,817 - -1.5 263,817 - -0.1 263,826 - +0.4 263,827 + +0.6 263,827 + +0.7 263,827 + -1.6 263,827 + +2.6 263,828 - -0.1 263,828 - +0.9 263,828 - +1.0 263,849 + -0.3 263,850 - -0.8 263,850 - +1.4 263,873 + +1.1 263,874 - -1.0 263,968 + -1.3 263,968 + +0.3 263,968 + -0.3 263,969 - -0.4 263,969 - -1.5 263,987 + DZA65_RS01170 0.11 +1.0 263,987 + DZA65_RS01170 0.11 +0.8 263,988 - DZA65_RS01170 0.11 -3.2 263,988 - DZA65_RS01170 0.11 +1.1 263,988 - DZA65_RS01170 0.11 -0.8 263,988 - DZA65_RS01170 0.11 -0.5 263,988 - DZA65_RS01170 0.11 +0.9 263,988 - DZA65_RS01170 0.11 +0.6 263,993 + DZA65_RS01170 0.12 -0.1 263,993 + DZA65_RS01170 0.12 +0.6 263,993 + DZA65_RS01170 0.12 +1.5 263,993 + DZA65_RS01170 0.12 +0.2 263,993 + DZA65_RS01170 0.12 -0.9 263,994 - DZA65_RS01170 0.12 +0.1 263,994 - DZA65_RS01170 0.12 +1.1 263,994 - DZA65_RS01170 0.12 -0.4 264,050 - DZA65_RS01170 0.17 +1.3 264,050 - DZA65_RS01170 0.17 +1.0 264,120 + DZA65_RS01170 0.23 +1.3 264,120 + DZA65_RS01170 0.23 -0.1 264,121 - DZA65_RS01170 0.24 +1.7 264,189 + DZA65_RS01170 0.30 -1.4 264,189 + DZA65_RS01170 0.30 -1.4 264,189 + DZA65_RS01170 0.30 +0.1 264,190 - DZA65_RS01170 0.30 +0.8 264,190 - DZA65_RS01170 0.30 +1.2 264,190 - DZA65_RS01170 0.30 +1.2 264,190 - DZA65_RS01170 0.30 +0.1 264,198 - DZA65_RS01170 0.31 +0.1 264,287 + DZA65_RS01170 0.39 +0.8 264,288 - DZA65_RS01170 0.39 -0.1 264,290 + DZA65_RS01170 0.39 -0.5 264,291 - DZA65_RS01170 0.39 +1.2 264,291 - DZA65_RS01170 0.39 +0.2 264,453 + DZA65_RS01170 0.54 +0.1 264,453 + DZA65_RS01170 0.54 -0.3 264,453 + DZA65_RS01170 0.54 -0.4 264,454 - DZA65_RS01170 0.54 +0.5 264,454 - DZA65_RS01170 0.54 -1.4 264,454 - DZA65_RS01170 0.54 -0.2 264,454 - DZA65_RS01170 0.54 +1.4 264,456 - DZA65_RS01170 0.54 -0.4 264,753 - DZA65_RS01170 0.81 -0.9 264,766 - DZA65_RS01170 0.82 +0.5 264,766 - DZA65_RS01170 0.82 -0.6 264,766 - DZA65_RS01170 0.82 +1.6 264,789 + DZA65_RS01170 0.84 +0.6 264,789 + DZA65_RS01170 0.84 +0.3 264,789 + DZA65_RS01170 0.84 -0.6 264,789 + DZA65_RS01170 0.84 -0.4 264,789 + DZA65_RS01170 0.84 +1.1 264,790 - DZA65_RS01170 0.85 -0.8 264,790 - DZA65_RS01170 0.85 +0.4 264,790 - DZA65_RS01170 0.85 +0.6 264,790 - DZA65_RS01170 0.85 +0.9 264,854 + +0.2 264,855 - -0.4 264,876 + +0.0 264,876 + -0.3 264,876 + -0.5 264,877 - -1.0 264,877 - +0.2 264,877 - +0.8 264,882 + -0.8 264,883 - +0.0 264,883 - +0.8 264,883 - -1.9 264,883 - -3.0 264,896 + -2.2 264,896 + -1.5 264,896 + -1.4 264,897 - +0.1 264,897 - +1.2 265,039 - +0.4 265,039 - -1.1 265,057 + +0.2 265,074 + +1.9 265,074 + -0.6 265,075 - -0.5 265,075 - -0.4 265,075 - +1.0 265,075 - +1.1 265,143 - +0.4 265,143 - +1.0 265,143 - +1.4 265,151 + -1.4 265,151 + +0.5 265,152 - -0.4 265,152 - -2.4 265,154 + -1.4 265,208 + -1.4 265,297 + DZA65_RS01175 0.34 +0.6 265,297 + DZA65_RS01175 0.34 -1.2 265,297 + DZA65_RS01175 0.34 +0.9 265,297 + DZA65_RS01175 0.34 -0.6 265,297 + DZA65_RS01175 0.34 +0.1 265,298 - DZA65_RS01175 0.35 -1.1 265,298 - DZA65_RS01175 0.35 -2.6 265,301 + DZA65_RS01175 0.37 -0.3 265,301 + DZA65_RS01175 0.37 -0.3 265,301 + DZA65_RS01175 0.37 +0.1 265,302 - DZA65_RS01175 0.37 +0.2 265,302 - DZA65_RS01175 0.37 -1.4 265,302 - DZA65_RS01175 0.37 -0.2 265,302 - DZA65_RS01175 0.37 +0.3 265,344 + DZA65_RS01175 0.61 -0.5 265,344 + DZA65_RS01175 0.61 +0.7 265,401 + -0.3 265,401 + +0.5 265,421 - +0.0 265,439 + -0.7 265,440 - +0.6 265,440 - +0.2 265,442 + +1.0 265,442 + +0.3 265,442 + -1.7 265,442 + -0.1 265,443 - -0.8 265,443 - -0.4 265,443 - +0.2 265,443 - -1.2 265,472 + +0.6 265,472 + +0.3 265,502 + +0.9 265,503 - -0.1 265,518 + -0.3 265,519 - -1.4 265,632 - -0.6 265,650 + -0.8 265,650 + -0.1 265,651 - +0.0 265,651 - -0.5 265,718 + DZA65_RS01180 0.23 +0.3 265,745 + DZA65_RS01180 0.37 -0.6 265,854 + -0.1 265,854 + +1.0 265,854 + +1.1 265,854 + +0.1 265,854 + -1.9 265,854 + -0.7 265,854 + -0.0 265,854 + -0.5 265,854 + +0.2 265,854 + +0.6 265,854 + +0.7 265,854 + -0.0 265,854 + +0.6 265,855 - +0.4 265,855 - -0.1 265,855 - -0.8 265,855 - -0.4 265,855 - -0.0 265,857 + +1.0 265,857 + +0.9 265,857 + -0.1 265,857 + -0.6 265,858 - +0.1 265,858 - +0.9 265,858 - -0.6 265,858 - +0.3 265,858 - +0.1 265,858 - -0.3 265,858 - +0.0 265,858 - +0.1 265,893 + +0.7 265,893 + -0.6 265,893 + -0.2 265,893 + +1.6 265,893 + +0.0 265,893 + +1.8 265,893 + +0.4 265,894 - -0.2 265,894 - +0.6 265,894 - +0.8 265,894 - -0.5 265,894 - +0.5 265,894 - +1.1 265,894 - -1.4 265,894 - -0.5 265,894 - -0.3 265,894 - +0.0 265,894 - -0.7 265,916 + DZA65_RS22770 0.18 -1.1
Or see this region's nucleotide sequence