Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01855 and DZA65_RS22780 are separated by 230 nucleotides DZA65_RS22780 and DZA65_RS01860 are separated by 40 nucleotides
DZA65_RS01855: DZA65_RS01855 - aminoimidazole riboside kinase, at 405,836 to 406,759
_RS01855
DZA65_RS22780: DZA65_RS22780 - hypothetical protein, at 406,990 to 407,253
_RS22780
DZA65_RS01860: DZA65_RS01860 - type I-E CRISPR-associated protein Cse1/CasA, at 407,294 to 408,829
_RS01860
Position (kb) 406
407
408 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 406.332 kb on - strand, within DZA65_RS01855 at 406.418 kb on + strand, within DZA65_RS01855 at 406.418 kb on + strand, within DZA65_RS01855 at 406.418 kb on + strand, within DZA65_RS01855 at 406.418 kb on + strand, within DZA65_RS01855 at 406.543 kb on - strand, within DZA65_RS01855 at 406.543 kb on - strand, within DZA65_RS01855 at 406.543 kb on - strand, within DZA65_RS01855 at 406.543 kb on - strand, within DZA65_RS01855 at 406.543 kb on - strand, within DZA65_RS01855 at 406.739 kb on + strand at 406.745 kb on + strand at 406.745 kb on + strand at 406.746 kb on - strand at 406.746 kb on - strand at 406.750 kb on + strand at 406.751 kb on - strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.760 kb on - strand at 406.760 kb on - strand at 406.760 kb on - strand at 406.763 kb on + strand at 406.763 kb on + strand at 406.763 kb on + strand at 406.763 kb on + strand at 406.763 kb on + strand at 406.763 kb on + strand at 406.764 kb on - strand at 406.764 kb on - strand at 406.764 kb on - strand at 406.764 kb on - strand at 406.764 kb on - strand at 406.764 kb on - strand at 406.787 kb on + strand at 406.796 kb on + strand at 406.797 kb on - strand at 406.800 kb on + strand at 406.800 kb on + strand at 406.800 kb on + strand at 406.800 kb on + strand at 406.800 kb on + strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.926 kb on + strand at 406.933 kb on + strand at 406.933 kb on + strand at 406.934 kb on - strand at 406.934 kb on - strand at 406.934 kb on - strand at 406.934 kb on - strand at 406.934 kb on - strand at 406.959 kb on - strand at 406.974 kb on + strand at 406.974 kb on + strand at 406.987 kb on + strand at 406.987 kb on + strand at 406.987 kb on + strand at 406.987 kb on + strand at 406.987 kb on + strand at 406.987 kb on + strand at 406.988 kb on - strand at 406.988 kb on - strand at 406.988 kb on - strand at 406.988 kb on - strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.992 kb on - strand at 406.992 kb on - strand at 406.992 kb on - strand at 406.992 kb on - strand at 406.992 kb on - strand at 406.994 kb on + strand at 406.994 kb on + strand at 406.995 kb on - strand at 406.995 kb on - strand at 406.995 kb on - strand at 406.995 kb on - strand at 406.997 kb on + strand at 406.997 kb on + strand at 406.997 kb on + strand at 406.997 kb on + strand at 406.997 kb on + strand at 406.998 kb on - strand at 406.998 kb on - strand at 406.998 kb on - strand at 406.998 kb on - strand at 406.998 kb on - strand at 406.998 kb on - strand at 407.010 kb on + strand at 407.010 kb on + strand at 407.011 kb on - strand at 407.011 kb on - strand at 407.021 kb on + strand, within DZA65_RS22780 at 407.022 kb on - strand, within DZA65_RS22780 at 407.068 kb on - strand, within DZA65_RS22780 at 407.077 kb on - strand, within DZA65_RS22780 at 407.080 kb on - strand, within DZA65_RS22780 at 407.109 kb on - strand, within DZA65_RS22780 at 407.124 kb on + strand, within DZA65_RS22780 at 407.124 kb on + strand, within DZA65_RS22780 at 407.125 kb on - strand, within DZA65_RS22780 at 407.136 kb on - strand, within DZA65_RS22780 at 407.136 kb on - strand, within DZA65_RS22780 at 407.136 kb on - strand, within DZA65_RS22780 at 407.136 kb on - strand, within DZA65_RS22780 at 407.276 kb on + strand at 407.277 kb on - strand at 407.287 kb on + strand at 407.287 kb on + strand at 407.287 kb on + strand at 407.287 kb on + strand at 407.288 kb on - strand at 407.288 kb on - strand at 407.288 kb on - strand at 407.288 kb on - strand at 407.356 kb on + strand at 407.357 kb on - strand at 407.357 kb on - strand at 407.357 kb on - strand at 407.465 kb on + strand, within DZA65_RS01860 at 407.466 kb on - strand, within DZA65_RS01860 at 407.678 kb on - strand, within DZA65_RS01860 at 407.678 kb on - strand, within DZA65_RS01860 at 407.744 kb on + strand, within DZA65_RS01860 at 407.744 kb on + strand, within DZA65_RS01860 at 407.745 kb on - strand, within DZA65_RS01860 at 407.812 kb on + strand at 407.812 kb on + strand, within DZA65_RS01860 at 407.812 kb on + strand, within DZA65_RS01860 at 407.813 kb on - strand, within DZA65_RS01860 at 407.813 kb on - strand, within DZA65_RS01860 at 407.834 kb on + strand, within DZA65_RS01860 at 407.834 kb on + strand, within DZA65_RS01860 at 407.834 kb on + strand, within DZA65_RS01860 at 407.835 kb on - strand, within DZA65_RS01860 at 407.835 kb on - strand, within DZA65_RS01860 at 407.884 kb on + strand, within DZA65_RS01860 at 407.885 kb on - strand, within DZA65_RS01860 at 408.043 kb on + strand at 408.102 kb on - strand, within DZA65_RS01860 at 408.102 kb on - strand, within DZA65_RS01860 at 408.116 kb on + strand, within DZA65_RS01860 at 408.128 kb on + strand, within DZA65_RS01860 at 408.158 kb on + strand, within DZA65_RS01860
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 406,332 - DZA65_RS01855 0.54 -0.3 406,418 + DZA65_RS01855 0.63 +1.0 406,418 + DZA65_RS01855 0.63 -0.8 406,418 + DZA65_RS01855 0.63 +0.9 406,418 + DZA65_RS01855 0.63 -1.6 406,543 - DZA65_RS01855 0.77 -2.6 406,543 - DZA65_RS01855 0.77 -0.4 406,543 - DZA65_RS01855 0.77 +0.6 406,543 - DZA65_RS01855 0.77 -0.0 406,543 - DZA65_RS01855 0.77 -0.6 406,739 + +0.9 406,745 + -1.0 406,745 + +0.3 406,746 - -1.2 406,746 - -0.1 406,750 + -0.6 406,751 - -0.3 406,759 + -0.1 406,759 + -1.1 406,759 + +1.1 406,759 + +0.4 406,759 + -0.4 406,759 + -1.3 406,759 + +1.5 406,759 + -0.5 406,759 + -0.3 406,759 + -0.1 406,759 + -0.5 406,759 + -0.1 406,759 + +2.5 406,760 - +0.7 406,760 - +0.0 406,760 - +1.2 406,763 + +0.5 406,763 + +0.5 406,763 + +0.0 406,763 + +0.8 406,763 + +0.0 406,763 + +0.9 406,764 - +0.3 406,764 - -0.9 406,764 - -0.1 406,764 - +0.3 406,764 - +0.5 406,764 - -0.9 406,787 + +0.3 406,796 + -0.8 406,797 - -0.7 406,800 + +0.3 406,800 + -2.0 406,800 + +1.2 406,800 + +0.9 406,800 + +0.2 406,801 - +0.2 406,801 - +0.0 406,801 - +0.2 406,801 - +0.6 406,801 - -0.7 406,801 - +0.1 406,801 - -0.5 406,801 - +0.2 406,801 - -0.2 406,926 + +0.4 406,933 + -0.4 406,933 + -0.2 406,934 - +0.3 406,934 - +0.1 406,934 - -0.1 406,934 - -0.3 406,934 - -2.6 406,959 - +1.0 406,974 + -0.5 406,974 + +1.4 406,987 + +1.0 406,987 + -0.5 406,987 + -0.8 406,987 + +0.3 406,987 + +0.5 406,987 + +0.8 406,988 - +0.8 406,988 - +1.0 406,988 - -0.9 406,988 - -0.3 406,991 + -1.3 406,991 + -1.5 406,991 + +2.4 406,991 + -0.4 406,991 + -1.0 406,991 + +1.7 406,991 + -0.1 406,991 + -2.0 406,991 + +0.5 406,992 - -0.2 406,992 - -0.5 406,992 - -0.5 406,992 - -2.0 406,992 - +0.7 406,994 + -0.5 406,994 + +0.3 406,995 - +0.0 406,995 - -3.0 406,995 - -0.0 406,995 - +0.3 406,997 + +1.1 406,997 + +0.1 406,997 + -1.7 406,997 + +1.1 406,997 + -0.5 406,998 - +2.0 406,998 - -1.3 406,998 - -0.0 406,998 - -0.1 406,998 - +0.5 406,998 - -0.0 407,010 + +0.1 407,010 + +1.5 407,011 - +0.7 407,011 - +0.2 407,021 + DZA65_RS22780 0.12 -0.5 407,022 - DZA65_RS22780 0.12 -0.2 407,068 - DZA65_RS22780 0.30 -0.9 407,077 - DZA65_RS22780 0.33 +0.1 407,080 - DZA65_RS22780 0.34 +1.4 407,109 - DZA65_RS22780 0.45 -0.5 407,124 + DZA65_RS22780 0.51 -0.2 407,124 + DZA65_RS22780 0.51 -1.5 407,125 - DZA65_RS22780 0.51 -1.0 407,136 - DZA65_RS22780 0.55 -0.5 407,136 - DZA65_RS22780 0.55 -1.5 407,136 - DZA65_RS22780 0.55 -2.3 407,136 - DZA65_RS22780 0.55 +0.8 407,276 + +0.5 407,277 - -1.0 407,287 + +1.6 407,287 + +0.2 407,287 + +1.3 407,287 + +0.1 407,288 - -0.5 407,288 - +0.5 407,288 - -1.5 407,288 - +0.6 407,356 + +0.5 407,357 - -0.2 407,357 - -0.5 407,357 - +0.6 407,465 + DZA65_RS01860 0.11 +0.9 407,466 - DZA65_RS01860 0.11 +1.4 407,678 - DZA65_RS01860 0.25 -1.8 407,678 - DZA65_RS01860 0.25 -0.1 407,744 + DZA65_RS01860 0.29 -0.3 407,744 + DZA65_RS01860 0.29 +0.5 407,745 - DZA65_RS01860 0.29 -0.8 407,812 + +1.0 407,812 + DZA65_RS01860 0.34 +0.8 407,812 + DZA65_RS01860 0.34 +0.2 407,813 - DZA65_RS01860 0.34 +0.5 407,813 - DZA65_RS01860 0.34 +0.1 407,834 + DZA65_RS01860 0.35 +0.2 407,834 + DZA65_RS01860 0.35 -0.0 407,834 + DZA65_RS01860 0.35 +0.8 407,835 - DZA65_RS01860 0.35 +0.3 407,835 - DZA65_RS01860 0.35 +0.6 407,884 + DZA65_RS01860 0.38 +0.7 407,885 - DZA65_RS01860 0.38 +0.0 408,043 + +2.4 408,102 - DZA65_RS01860 0.53 +0.1 408,102 - DZA65_RS01860 0.53 -0.3 408,116 + DZA65_RS01860 0.54 -0.9 408,128 + DZA65_RS01860 0.54 +0.1 408,158 + DZA65_RS01860 0.56 +0.4
Or see this region's nucleotide sequence